hellolet’s get started done by shnormal glomerulus with …€¦ · wire loops (حلقة غير...
TRANSCRIPT
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HelloLet’s get started
Done by SH
Normal glomerulus with capillary tubule & mesangial cells in between. (H&E stain)
لا يجوز ان يكون اكثر من ٣ خلايا mesangial بجانب بعض بالوضع الطبيعي.
في حالة وجود اكثر من ٣ خلايا mesangial بجانب بعض تصبح اسم الحالةMesangial hypercellularity
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PAS stain (Periodic acid–Schiff) —-> تغمق collagen & Basement membrane
Note —> mason trichrome stain fibrosis in blue colour.
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Electron micrography (Normal pic)
Podocyte foot processes
Fenestrated endothelium
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Effacement of foot processes in MCD (Minimal change disease)
Electron dense RBC
BM
Fenestrated endothelium
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Obliterated capillary, Segmental (part of glomerulus) sclerosis (fibrosis)
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Same as previous slide but different stain (Mason trichrome stain)
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Hypercellular (Proliferative) glomerulus
Small cells are neutrophils with segmented nucleus (inside glomerulus)Lobulated structure (closed lobes)
We think of poststreptococcal/postinfectious GNOr Memranoproliferative GN
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Same as previous slide but on high magnificationNotice neutrophils in here clearly
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Subepithelial humps (btw BM & podocyte)Post streptococcal GN feature
Capillary lumen
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Immunoflorescence (capillary granular)In many diseases such as membranous nephropathy
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H&E stain, thickening in capillary lobes (BM) Diffused
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Silver stain, BM appear in black
Spikes appear here in black (على شكل شعيرات صغيرة) in membranous nephropathy
Note —-> Amyloid appear in Congo Red stain
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Capillary granular in IF
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Capillary granular in IF
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PAS stain (fusci color fibrosis on BM & around tubules)
Crescent proliferation in urinary space (parietal cells)
Wire loops (حلقة غير مكتملة ذات جدار سميك) mainly in active lupus nephritis class III & IV
Tubule
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H&E stain (bad stain)Crescent proliferation may convert to fibrosis (Pauci-immune)
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Capillary Linear in If
Goodpasture syndrome (only appear as linear)
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Mesangial hypercellularity (اكثر من ٣ خلايا بجانب بعض)In IgA nephropathy
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Mesangial granular in IfMesangial always granularتكتلات عشوائية
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Same as previous slide
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Glomerulus with a lot of cells (closed)no neutrophils (so no postinfectious/post streptococcal GN)Maybe membranoproliferative GN
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Silver stain, Tram-truck appearance (double circular line)In membranoproliferative GN