HEMATOLOGY AUTOMATION

Principles of Automated Cell Counts

Electronic Impedance

Optical Scatter

Radiofrequency

Flow Cytometry

Hydrodynamic Focusing

Laminar Flow

Electronic Impedance

Changes in electrical resistance between two electrodes as cells pass through a sensing aperture

Voltage changes

Low voltage direct current resistance

Electronic Impedance

Radiofrequency

High voltage electromagnetic current

Measurable changes in RF signal proportional to cell interior density or conductivity

Optical Scatter

Manufacturers

Beckman Coulter

Sysmex

Abbott

Siemens

ABX

Machine Principles

Histograms

Histograms

Histograms

Scattergram

Troubleshooting

Troubleshooting

Quality Assurance

Preanalytic, Analytic and Postanalytic

Quality Control- internal and external

Statistical QC