histology lab slides
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Histology Lab SlidesTRANSCRIPT
![Page 1: Histology Lab Slides](https://reader033.vdocuments.net/reader033/viewer/2022050719/54992208b479591c488b46db/html5/thumbnails/1.jpg)
Epithelial Tissue
• Characteristics:
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Simple Cuboidal
• Description: Single layer of cube-shaped cells.
• Location: small ducts of glands, kidney tubules, surface of ovary.
• Function: secretion & absorption
Cell is as wide as it is high. Onecentral nucleus
(= single layer)
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Simple Cuboidal
• Cells closely packed together and touching one another
• Has a basal surface and apical surface
• Avascular
• Sits on a basement membrane (difficult to see on slide)
Lumen:Inside space of Tubular structure
Nucleus ApicalSurface
BasalSurface
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Non- Ciliated Simple Columnar• Description: Single
layer of non-ciliated rectangular cells; contain goblet cells and sometimes microvilli.
• Location: GI tract, ducts, gallbladder
• Function: secretion and absorption
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Goblet Cell
Nucleus
Goblet Cell
• Nuclei lie near base of cell• Goblets secrete mucous at
apical surface to lubricate lining of GI, respiratory, reproductive and urinary systems.
• Microvilli increase surface area for absorption.
Simple Columnar Cells
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Pseudostratified Ciliated Columnar Epithelium
• Appears stratified because nuclei are at varying levels.
• Ciliated lines airways of upper respiratory tract.
• Mucus from Goblets traps foreign particles and are swept away by cilia.
Goblet Cells
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Cilia
Nuclei at varying levels
Goblet Cells secreting mucus
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Epithelial and Connective
• 1 – pseudostratified columnar epithelial
• 2- simple cuboidal epithelial
• 3- hyaline cartilage connective tissue
1
2
3
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Fibroblast CELLS
MATRIXFIBER
Elastic Fiber
Collagen Fiber (thicker pink ones)
GROUND SUBSTANCELoose Areolar
Connective Tissue
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Teased Dense Regular Connective Tissue
• Forms tendons and ligaments and aponeuroses.
• Matrix looks white and shiny.
• Mostly collagen fibers with fibroblasts between rows.
• Strong attachment!
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Teased Dense Regular CT (magnified)
Fibers are mostlyCollagen.
Fibroblasts
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Reticular Connective Tissue
• Network of interlacing reticular fibers & reticular cells
• Found in stroma (framework) of liver, spleen, lymph nodes, red bone marrow
• Acts as a filter in spleen and lymph nodes.
Blue stainingReticular fibers
Reticular Cells
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Reticular Connective Tissue
• Remember that connective tissue is vascular. (Avascular epithelial tissue depends on diffusion of nutrients and waste through blood vessels in CT.
Blood vessel:Single layer of Epithelial cells forms vessel tube.
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ReticularCT
• 1- Reticular cells
• 2- Blood vessel
• 3- Reticular fibers
3
1
2
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Hyaline Cartilage
• Often called gristle• Bluish white smokey
looking ground substance • Numerous chondrocytes• Location: ends of long
bones, ribs, nose, trachea, larynx, bronchi
• For flexibility and support• Most abundant type of
cartilage in body
chondrocytes
Ground Substance
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Elastic Cartilage
• Chondrocytes are located within threadlike network of elastic fibers
• Location: Epiglottis of larynx, external ear, Eustachian tubes
• For support and shape
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chondrocyte
Lacuna(hollow spaceholding chondrocyte)
Elastic vs Hyaline Cartilage
Elastic fibers throughoutGround substance
SmootherAppearanceOf groundsubstance
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AdiposeTissue
• Adipocytes, “signet ring” cells with peripheral nuclei.
• For storage of triglycerides, to reduce heat loss, and serve as an energy reserve.
• Location: under skin, around heart & kidneys, yellow marrow, behind eyeball
“signet ring” adipocyte
nucleus
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Osseous Tissue (Bone)• Compact bone consists of
osteons (haversion systems) that contain:– Lamellae– Lacuna– Osteocytes– Canaliculi– Central (haversion)canals
• Spongy bone consists of thin plates called trabeculae filled with red marrow
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HaversionSystem
Lamellae
Central(Haversion)Canal
Lacunae
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Lacunae (where osteocytes reside)
Canaliculi: small Tunnels where osteocyteprocesses run for diffusionof nutrients and wastes
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Skeletal Muscle Tissue
• Striated– Proteins
• Actin• Myosin
• Multinucleated• Voluntary• Attach to bones• For movement,
communication, posture, heat production
Striations
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Skeletal muscle fiber with multiple nuclei
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Smooth (Visceral) Muscle Tissue• No striations• Spindle-shaped• Single centrally located
nucleus• Nucleus is long and cigar
shaped• Involuntary• Viscera• Walls of hollow structures
– Blood vessels– Airways to lungs– Stomach– Intestines– Gallbladder– Urinary bladder
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Cigar shaped nuclei
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Smooth Muscle
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Cardiac Muscle Tissue
• Striated, mono-nucleated, branching
• Self excitatory• Intercalated discs
attach fibers together and contain gap junctions (help with conduction of electrical signals)
Intercalated disc
nucleus
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Name the tissues and structures you see.
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Simple Squamous Epithelial
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Stratified Squamous Epithelial
These keratinized epithelial cells willBe sloughed off.
Basal Layer
Apical Layer