histology. light microscopy (lm) – paraffin embedded, 7 micron sections, specific stains...
TRANSCRIPT
![Page 1: Histology. Light Microscopy (LM) – paraffin embedded, 7 micron sections, specific stains Transmission Electron Microscopy (TEM) – plastic embedded, 70](https://reader037.vdocuments.net/reader037/viewer/2022103122/56649f435503460f94c630bd/html5/thumbnails/1.jpg)
Histology
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• Light Microscopy (LM) – paraffin embedded, 7 micron sections, specific stains
• Transmission Electron Microscopy (TEM) – plastic embedded, 70 millimicron sections, e-dense stains
• Scanning Electron Microscopy (SEM) – critically point dried, gold coated
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Light Microscopy
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TEM
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SEM
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Epithelium
Simple Epithelium
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Simple Squamous
Mesothelium
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Simple Squamous (Mesothelium) by TEM
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Simple Squamous – Endothelium (En)
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Simple Squamous – Endothelium (Ed) by TEM
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Capillary (endothelial cell) by TEM
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Simple Cuboidal Epithelium (longitudinal section)
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Simple Cuboidal (cross section)
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Simple Cuboidal by TEM
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Simple Columnar with Goblet Cells
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Simple Columnar (cross section)
with Goblet Cells (arrow)
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Goblet Cell with PAS stain (red)
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Simple Columnar with Goblet Cells
(Mu) by TEM
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Simple Columnar (AC) with Goblet Cells
(GC) by SEM
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Simple Columnar mucus
secreting
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Simple Columnar mucus
secreting
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Epithelium
Stratified Epithelium
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Pseudostratified Ciliated Epithelium with Goblet Cells
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Pseudostratified Epithelium with Cilia (a) and Goblet Cells (b)
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Stratified Squamous non-Keratinized Epithelium
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Transitional Epithelium
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Transitional Epithelium with rounded binucleated surface cells
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Stratified Squamous Keratinized (thin)
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Stratified Squamous Keratinized (thick)
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Epithelial Cell Junctions
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Tight Junctions (Zonula Occludens) by TEM
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Tight Junctions (Zonula Occludens) by Freeze Fracture
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Gap Junction (Nexus) by TEM
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Gap Junction
Connexin moleculesOpen pores for
ions, molecules, etc
2 nM gap between membranes
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TEM of Zonula Adherens (red
arrows)
TEM of a Desmosome (blue
arrow)
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TEM (colorized) of Adherens Junctions
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TEM of a Desmosome with tonofibrils
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TEM of a Hemi-Desmosome
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Summary of the maintenance of cell shape by junctions and cytoskeletal elements
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Glandular Epithelium
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Serous Gland with ducts (arrows)
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Serous secretory unit (high magnification)
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Mucous Gland with duct (arrow)
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Mixed Gland with Serous Demilunes (SD)