how to manage a biotech lab ellyn daugherty sm biotech career pathway
TRANSCRIPT
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How to Manage a Biotech Lab
Ellyn DaughertySM Biotech Career Pathway
www.SMBiotech.com
www.BiotechEd.comwww.emcp.com/biotech
www.sargentwelch.com/biotech
650-400-9424
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Things to Consider:
• Lay-out/Workflow/Storage• Lab Stations• Common Work Areas• Chemicals/Chemical Storage• Refrigerator/Freezer Storage• Student Sample Storage• Waste Disposal• Other Safety Issues• Inventory/Ordering• Other Issues
Setting up and Managing a Biotech Lab
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• Student movement, bottle necks, & time constraints
• Set up at individual lab stations vs. common work areas
• Replicate work areas - avoid long waits and “long distance”
• Strategically placed tables, carts, deionized water, sinks
• Gas/Bunsen burner placement?
• Storage areas - immediate, store-term, and long-term
Facility Lay-out/Workflow/Storage
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• Students work at lab station in pairs (lab partners)
• 2 pairs at a lab station (lab table)
• A minimum on the lab table
> 1 hot plate stirrer/lab station
> 1 spectrophotometer/lab station
> a trash bucket/lab station
> 1 (serological) pipet rack/table
> 1 micropipet stand/table
> a box of gloves/table
• Strategic use of drawers and cabinets
> Small instrument drawer (pipet pumps, micropipet tips, pen, tape, scissors,
goggles, etc.)
> Student storage drawer
> Small equipment & reagent cabinet(gel boxes, power supplies, large volumes of buffer, etc.)
Lab Stations
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Common Work Areas• In strategic locations – common work stations
> electronic & analytical weighing stations
> pH meter/pH adjustment station
> centrifuge station, UV spec station
> refrigerators and freezers (mini-?)
> gel staining and visualization station
> incubation ovens, water baths,
heat blocks, microwaves
> deionized water (dH20)
> autoclaves and drying ovens
> supply tables/supply carts
• Hoods
> Laminar flow hoods vs. bio-safety cabinets(for sterile work, protect user and samples)
> Chemical fume hoods(for dispensing organics, caustics, flammables, noxious)
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Chemicals & Chemical Storage
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Refrigerator/Freezer Storage
• Most samples are labeled with storage temp• Virtually all protein, DNA, and cell samples must
be stored at 4°C (refrig) or at -20°C (lab freezer)
> Store lyophilized (powdered) protein or DNA samples at -20°C, unless otherwise labeled
> Store protein samples alphabetically
> Record the date on reagents upon arrival
> Certain cell lines must be stored at -70°C or below or lose competence or viability
• Do not use frost-free refrigerator/freezers• Repeated freezing/thawing compromises most
samples (aliquot samples on receipt into more usable volumes)
• If possible, have designated refrigerators and freezers for different courses
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Student Sample Storage
• Room Temp Storage
> chemicals, many buffers
• Refrigerator/Cooler (4°C) Storage
> most protein or DNA student samples
• Freezer (-20°C) Storage
> some protein or DNA student samples
• The more you can separate student samples the more time is saved
> Consider separate lab refrigerators and freezers (4.0 cu ft models) or different shelves in large volume coolers or freezers
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• Most districts have specific policies for waste disposal (find out about yours)
• Most hazardous waste must be collected and disposed of by professionals• Need biohazard bags for biological hazards = plates (no sharp items)• Autoclave bio-contaminated items 15-20 mins @15-20 psi before trash• Bio-contaminated loops and tubes can be soaked in 10% bleach for 30
min before regular trash
• Many chemicals may not go down drain (see district rules), ie. CuSO4, silver nitrate, EtBr?, etc
• Label waste with type/concentration/date
Waste Disposal
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• Gloves and goggles at every lab station and workstation, for all chem work • No gloves for work with Bunsen burner or microwave (burn hazard)
• Use hot hand protectors for hot bottles/beakers• Disinfectants at each hood (1x Lysol® or Amphyl®), where bacteria is used• Use of 10% bleach or 70% EtOH, where plant tissues are used• Use of lid-locks for 1.7 mL tubes and hot heat blocks• Safety shower, eyewash• Broken glass cartons, fire extinguisher at several places
Other Safety Issues
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• Keep a record (Excel® spreadsheet with vendor, manufacturer, description, package size, part
number, etc.) of all materials used and received.• Use inventory sheets to help
keep areas clean and organized
• Assign student groups the responsibility to maintain/inventory a particular area> Chem Stockroom A-G > Weigh Stations
> “Darwin” Refrigerator > “Watson”, “Crick” Freezers
• Have inventory sheets at each lab station and require inventory sign-off at the beginning and end of class (Biotech Live Ch 3)
• Have a place that students can record when a “last bottle” is ½ full (use to amend orders)
• Build time into the schedule for inventory and lab station/workplace maintenance
Inventory/Ordering
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• Computers – keep them away from chemicals and water
• Not enough space – portable items?• Alphabetized small items drawers or
containers• Cable-lock down balances, computers• Designated hand-washing sinks?• Lots of paper towels used• Crushed ice needed/preferred• Post emergency numbers• Biotech teacher “in charge” of facility• Keep getting professional development• Network with other biotech educators
and ask them questions (especially about new methods, techniques, equipment)
Other Issues
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Get even more help!
Ellyn DaughertySM Biotech Career Pathway
www.SMBiotech.com
www.BiotechEd.com
www.emcschool.com/biotechnology
www.sargentwelch.com/biotech
Amy NaumVWR Ed/Sargent Welch
www.sargentwelch.com/[email protected] 585-321-9422
Holly AhernVWR Ed/Sargent Welch
www.sargentwelch.com/[email protected] 512-789-3155