BY LAWS FOR PARA MEDICAL INSTITUTIONS CONDUCTING PARA MEDICAL COURSES IN DMLT/DMRT.

The institution shall be established as per Para Medical Council norms notified in Gazette.

The following Bylaws will govern the institution and their credibility. Schedule and the examination patter conducting body and hospital attachment.

1. (a) Candidate should be bonafide Himachali.(b) Candidate should be 10+2 with at least 50% marks in Physics,

Chemistry and Biology or mathematic combined together. (40% for SC/ST)

OrHave appeared in 10+2 examination can be considered provisionally till the declaration of result as clause 1 (b).

(c ) Should be less than 17 years and more than 25 years on the 1st

July of the year of admission.(d) Selection of the candidate shall be made strictly as per merit of

10+2 aggregate marks in Physics, Chemistry and Biology or mathematic combined together.

(e) Admission / examination conducting body shall be H.P Paramedical Council.

(f) Minimum 2 year duration of the course (DMLT/DMRT) wich includes 6 months apprentenship i.e. they are diploma course while degree course are of three years.

(g) The paramedical institutions in Private Sector who does not own their hospital can be attached to accordingly to the Government instruction / NOC (i) in the State Medical Colleges / District Hospital / Zonal hospital (ii) in the private hospital where hospital with minimum 100 beds attached. Only one institution will be attached with the health institutions at a particular location.

(h) The paramedical institutions should be organized with in the 30 Kms distance from the Hospital.

(i) The institution should have an own building / rental building.

TEACHING FACULTY FOR DMLT (FOR 20 STUDENTS)

Teaching faculty for DMLT (all teaching and technical staff should be registered with the HP Para Medical Council/State Medical Council as applicable) :-

Sr. No.

Designation Number of posts

Qualification (Regularly acquired from Government recognized institution).

DMLT

1. Principal 1 MD in (Biochemistry/Pathology/ Microbiology) and degree recognized by M.C.I

or

M.Sc. in Medical laboratory technology/ Medical biochemistry/ Medical Microbiology/ Medical Pathology with 2 year working experience or teaching experience after doing MSc.

2. Lecturer 1 MD in (Biochemistry/ Pathology/ Microbiology) and Degree recognized by M.C.I

or

M.Sc. in Medical laboratory technology/ Medical biochemistry/ Medical Microbiology/ Medical Pathology with 2 year working experience or teaching experience after doing M.Sc.

or

BSc. in Medical Laboratory Technology with 5 years of teaching experience or working

experience after doing B.Sc.

3. Tutor 2 B.Sc. in Medical Laboratory Technology

4. Technician 2 Diploma in laboratory Technology

(Two year Diploma Course)

5. Computer Teacher

1 PGDCA or One year Diploma in Computer Application

6. Lab Attended 2 Diploma (2 years) or Certificate course (1 year) in Laboratory Technology.

7. Clerk 1 10+2 having knowledge of typing (speed 30 WPM) and computer application.

8. Peon 1 Matriculation

9. Safai Karamchari 1 NIL

NOTE: -

1. 33% of faculty may be visiting amongst lecturer and Tutor combined together.

2. For every additional twenty student or part thereof up to the maximum, of forty students; the faculty and staff should be doubled except Computer operator.

Space : - Minimum requirement1. Principal room 120 Sq. Feet2. Staff Room 100 Sq. Feet3. Store 100 Sq. Feet with shelves for storage4. Seminar cum teaching 300 Sq. Feet and additional 200 Sq

feet for Room every 20 student or part thereof.

5. Laboratories 250 Sq. feet of space must be provided for Microbiology, Pathology and Biochemistry Laboratories separately and space double for every 20 additional students or part thereof.

6. First Aid Room 40 Sq. Feet7. Toilets i) One attached with Principal Room

ii) One for staff.iii) Two for students (one Ladies and one gents)

NOTE: -

1. Entire premises of the institution should be with in 500 meters ( i.e. diameter)

2. Teaching schedule : As prescribed by the HP Para Medical Council.3. Deficiency of upto a maximum of 10% is permissible in the floor area

of individual rooms and individual laboratories of the institution.

TEACHING FACULTY FOR DMRT (FOR 20 STUDENTS)

Teaching faculty for DMLT (all teaching and technical staff should be registered with the HP Para Medical Council/State Medical Council as applicable) :-

Sr. No.

Designation Number of posts

Qualification (Regularly acquired from Government recognized institution).

DMRT

1. Principal 1 MD/DMRD in Radio- diagnosis

or

M.Sc. in Radiology with 2 year experience in working/teaching after M.Sc.

2. Lecturer 1 MD /DMRD in Radio- diagnosis

or

M.Sc. in Radiology with 2 year experience in working/teaching

after M.Sc.

or

B.Sc Radiology Technology with 5 year experience in working/ teaching after B.Sc.

3. Tutor 2 B.Sc. Radiology Technology

4. Radiographer 2 Diploma in Radiology Technology

5. Computer Teacher

1 PGDCA/ One year Diploma in Computer Application

6. Clerk 1 10+2 having knowledge of typing and computer

7. Peon 1 Matriculation

8. Safai Karamchari 1 -

NOTE: -

1. 33% of faculty may be visiting amongst lecturer and Tutor combined together.

2. For every additional twenty students or part there of up to the maximum, of forty strength, the faculty and staff should be doubled except Computer operator.

Work load for twenty seats: - The hospital/ Diagnostic centre should perform minimum investigation as follows: -

10 to 20 X-rays per days5 ultra sound per days

Other special investigation: - Barium studies, IVP and other investigation major 3 per

day combined together.Or

The institution or Hospital/Diagnostic centre which cannot have CT scan, MRI and Mamography must get attachment either to a state run Zonal hospital/district hospital or well equipped private hospital where facilities for these modalities i.e. (CT Scan, MRI, mammography etc) exist.

Radiation and safety measure. The Hospital or the diagnostic centre has to get approval of the BARC of from Radiation safety Officer Appointment for the state by the BARC for radiation protection. The firm batch or TLD batch should be procured for all the staff members and students.

Space; - The Principal room, staff room, office, library, class room, first aid room and toilet facilities will be similar to these mentioned for DMLT courses.

For special investigations: 200 sq feet room

100 Sq feet for ultra sound room with

Waiting area of 100 Sq feet.

X-ray room if separate then 150 Sq Feet

CT Room 400 Sq feet(this will include toilet, main room and console room.

MRI Room As per company provision

Other requirement as prescribed by the Council.

CURRICULUM FOR TWO YEAR DIPLOMA IN

MEDICAL LABORATORY TECHNOLOGY

AND

MEDICAL RADIOLOGY TECHNOLOGY

(Prescribed by)

HP Paramedical Council

DIPLOMA IN MEDICAL LABORATORY TECHNOLOGY1. Salient Features

1.1 Duration of course: Two Years

1.2 Qualification (Minimum) 10+2 Pass with Physics, Chemistry, Mathematic or Biology from a recognized Board with 50% marks combined (40% for SC/ST).

1.3 Annual intake of students 20 or as approved by the H.P Paramedical Council/SCVT.

1.4 Programme Pattern Theory and practical for 36 Hours/week and Computer classes 2 Hours per week in first year of Training.

1.5 Practical training To develop practical skills in students in second year (last six months) by hands or training in medical collage/hospitals/nursing home having clinical laboratories, in private and Government sector.

2. Teaching and Examination Pattern.

2.1 Teaching pattern

(First Year)

Subject Hours per week

Theory Practical

Anatomy, physiology and basic Histopathology 4 5Clinical Hematology and Cytology 4 5Clinical Microbiology 4 5Clinical Biochemistry 4 5Computer Classes 1 1Total Teaching 17 + 21 =38 HoursSecond Year (first Six Months)

Subject Hours per week

Theory Practical

Histopathology 4 5

Clinical Hematology, Cytology and Blood Banking 4 5

Clinical Microbiology 4 5

Clinical Biochemistry 4 5

Total Teaching 16 + 20 =36 Hours

Second Year (Last Six Months)

Practical Training in Laboratories/Institutions for 36 Hours per week.

2.2 Examination Pattern :

At the end of the First Year :

Theory

Theory includes Written examination, Viva and Internal assesment

(Total marks 100 X4=400)

Written examination:

Paper 1 : Anatomy, physiology and basic 50 marks 3 Hours

Histopathology

Paper 2: Clinical Hematology and Cytology 50 marks 3 Hours

Paper 3: Clinical Microbiology 50 marks 3 Hours

Paper 4: Clinical Biochemistry 50 marks 3 Hours

Internal assessment 25 marks in each subject

Grand Viva 25 marks in each subject.

Total marks for each paper= 50+25+25=100

Practical examination : 100x4=400 marks.

Practical Test = 75 marks in each subject.

Internal assessment = 25 marks in each subject.

Total marks in each subject 100+100=200 marks.

Second Year

Theory includes Written examination, Viva and Internal assesment

(Total marks 100 X4=400)

Written examination:

Paper 1 : Histopathology 50 marks 3 Hours

Paper 2: Clinical Hematology and Cytology

And Blood banking 50 marks 3 Hours

Paper 3: Clinical Microbiology 50 marks 3 Hours

Paper 4: Clinical Biochemistry 50 marks 3 Hours

Internal assessment 25 marks in each subject

Grand Viva 25 marks in each subject.

Total marks for each paper= 50+25+25=100

Practical examination : 100x4=400 marks.

Practical Test = 75 marks in each subject.

Internal assessment = 25 marks in each subject.

Total marks in each subject 100+100=200 marks.

Subject wise Minimum passing percentage, Internal assessment and attendance .

Minimum passing percentage : 50% separately in Theory and Practical

Minimum Internal assessment : 35% separately in Theory and practical.

Minimum attendance: 75% separately in Theory and practical.

Syllabus for Diploma medical lab. Technology Diploma

(1st Year)PATHOLOGY BASIC ABATOMY PHYSIOLOGY

1. Introduction: - Introduction to human body.Cell structure and function.Cell Division.Tissues.

2. Hematopoietic System :-BloodBone marrowLymphnodes and spleen.Lymph and Lymphatic system.

3. Cardiovascular system : -Structure and functions of heart & blood.Circulation- Systemic

- PalmonaryCardiac cycle.Nerve supply of heart.Definition of blood pressure.

Cardiac output ECG.4. Respiratory System : -

Organs of Respiration and their histology.Physiology of Respiration.

5. Excretory System : -

Organs of Excretion (Kidney, Bladder and Ureter)

Structure and functions of kidney.

Physiology of excretion.

Formation of urine and its compositions.

6. Skin : -Structure and functions.

7. Digestive System :-Structure of G.I.T.Liver pancreas, Gall bladder, salivary glands.Physiology of Digestion.Compositions of Gastric or Pancreatic juices, bile, succus entericus.Vitamins and minerals.

8. Endocrine Glands : -

Structure functions.

Hormone secretion and their functions.

9. Reproductive System : -Male and Female genital system.Structure and functions.Histology of Gonads.Ovarian cycle. Ovulation.Fertilization.

10. CNS & PNS : -Structure and functions of CNS.

Brain and Spinal cord.Structure and functions of sense organs (Eye, Ears, Tongue and Nose).

11. Skeletal System : -

Skeletal important bones and their brief description, structure and bone.

Articulation of bones & joints.

12. Muscles & joints : -Structure and function of skeletal muscle.Smooth muscle and cardiac muscle.

Code Ethics For Lab Technician

- Responsibilities and duties of Medical Technicians.- Organization and requirement of Medical Techinicians.- Safety regulations and first aid in laboratory.- Maintenance of lab record.

Introduction to Laboratory equipment & basic Lab. Operations : -

- Microscope - Photocolorimeter- Autoclave and its operation.- Hot air Oven.- Centrifuge machine.- Water bath etc.

Cleansing of glassware : -

- New glass ware.- Dirty glass ware.- Glass pipettes.- Dirty slides.

- Cover slips.- Preparation of grease free cover slips.

Light Microscope use, care and maintenance : -

Specimen collection and Preservation: -

- Blood, fluids, sputum.- Biopsy materials- Smears-scrapings.- Urine, Stool, semen- Swabs.

Disposal of Lab infected material & Clinical specimens : -

- Stool, urine, sputum & others body fluids.- Surgical specimens (Disposal of department issue sent for H/E).- Burial of germs containing materials.- Operation of incubator.

Principal & preparation of reagents & solutions : -

Quality control of lab findings.

1. Histotechnology2. Introduction & Basic technology3. Lab equipment & its care4. Fixation of tissues : -

- Classification of fixatives- Simple fixatives and their properties.- Microanatomical fixatives.

5. Tissue Processing : -- Collection, labeling and fixations of specimens.- Processing of bones tissue, fixation and decalcification- Dehydration

- Cleaning - Impregnation- Embedding

6. Section cutting : -- Microtome & microtome knives- Knife sharpening- Section cutting & labeling of slides

7. Staining & Mounting : -- Dyes and their properties- Theory of staining & techniques with Maematoxyline & Eosin.- Mounting

8. Museum techniques : -- Preparation of specimen- Preparation of fixative- Preparation of colour.- Preservation, Presentation & labeling

9. Clinical Pathology : -- Haemogram- Urine examination- Stool examination- Sputum for AFB

HAEMATOLOGYOrganization of Haematology laboratory

Introduction to Haematology

Components of blood and their functions

Hematopoietic system

Erythropoiesis

Gramnulopoiesis

Thrombopoeses

Normal Haematology values in health.Specimen collection & lab preparation

- Venipuncture- Cleaning f glassware in Haematology- Care of syringes & Needles- Cleaning of blood tubes, pipette, slides, cover slips- Anticoagulants

Basic Hematological techniques : -- Methods of Hemoglobin estimation- Acid haematin- Alkali haematin- Cynmethaemoglobin- Hemoglobin estimation- Clinical significance of test- Various counting chamber- RBC Count- Total leucocytes count- Platelet count- Reticulocyle count

PCV Micro methodMacro method

ESR estimation and its clinical significance : -

- Wintrobe’s method- Westergren’s method- Micro methods- Their clinical significance- Preparation of P/s Thick & Thin- Straight loucoyte edge

- Staining techniques- Romanowsky’s stain preparation theory- Field’s stain- Automation in haematology- Determination of RBC indices : - MCV, MCH, MCHC- Leterus/index

IMMUNO HEAMATOLOGY & BLOOD BANKING II1. Planning-Organization of Blood Banks and record keeping.2. Blood donation

- Donor screening - Collection, preservation of blood- Anticoagulants- Storage of blood

3. ABO Blood Group system : -- Sub groups- Inheritance- Type of antibodies- Secretors

4. Rh Blood group system : -- Nomenclature- Inheritance- Type of antibodies- Rh incompatibility

5. Techniques of groups & cross matching : -- Forward & Reverse grouping

6. Preparation of washed RBS suspension7. Gross matching Major

Minor

1. Introduction & basic terminology in cytology2. Lab equipment for cytology

Care of frequently used equipment3. Collection & preparation of cytological specimen

i. FNACii. Exfoliative Cytology

a) Cervicovaginal smearb) Fluids

- Plecural- Pericardial- Peritoneal

c) Brushing & washingd) Urinee) Sputumf) Fixatives in Cytology

4. Techniques preparation, fixation & staining of smear5. Preparation of stains in Cytology

- Romanowsky’s Stain- Papanicolaou Stain- Difference Quick stain

6. Techniques of staining

PRACTICALS BASED ON ANATOMY AND PHYSILOGY- Demonstration of body parts- Demonstration of tissue- Demonstration of parts of digestive system- Demonstration of respiratory system- Demonstration of skin- Demonstration of excretory system- Demonstration of circulatory system- Demonstration of blood films for blood cells- Demonstration of various parts of CNS- Difference between skeletal muscle, smooth muscle and cardiac

muscles- Demonstration of various bones and joints

- Demonstration reproductive system

(Demonstration can be done with the help of models charts and histological slides)

PRACTICAL

A.General - Based on theory- Microscope, use, care & maintenance- Microscope optics- Cleaning of glassware, preparation of dichromate solution

B. HISTOTECHNOLOGY I. Fixation, processing, embedding, section, cutting : -

1. Preparation of fixative2. Preparation god decalcifying solutions3. Preparation of different concentration of alcohols for tissue

processing4. Tissue processing5. Knife sharpening6. Section cutting7. Preparation of adhesives to fix section on slides8. Preparation of stains/reagents for H&E staining.9. Staining techniques10. Mounting and labeling

II. MUSEUM TECHINQUES 1. Mounting of specimens2. Preparation of fixatives for mounting

C. CLINICAL PATHOLOGY - Routine Urine examination including specific gravity estimation

- Routine stool examinationD. HAEMATOLOGY

- Preparation of anticoagulant solution- Preparation of vials for collection of blood- Venipuncture and collection of blood samples - Preparation and fixation of thick and thin blood smears

I. ESTIMATION OF HAEMOGLOBIN - Preparation of N/10 Hel.- WBC & RBC diluting fluids- Fluids for platelet count- RBC count- Total leucocyte count- Estimation of ESR- Winstrobe’s tude

- Westergran’s pipette- Estimation of PCV.

- Staining of P/S with Romanowsky stain

II. IMMUNO HAEMATOLOGY - ABO GROUPING- Slide method- Tube method- Cross matching

Major

Minor

E. CYTOLOGY : -

1. Preparation of Giemsa stains2. Preparation of reagents for PAP stain3. Preparation of FNA Smear4. Processing of fluid specimen including CSF & Urine for cytological

examination.

5. Staining of smears- Romanowsky’s Stain- PAP stain

2 nd Year Syllabus for DMLT

HISTOPATHOLOGY

1. Heading and embedding of small tissues, fragment and bones2. Special stains : -

- Connective tissue- Fat & Lipids- Carbohydrates & Mucoproteins- Bacteria- Fungi- Inclusion bodies

Special reference to : -

- PTAH, Van – Giesson’s, Masson’s trichrome- Reticulin stain- Verhoff’s elastic stain- Fontana – masson’s stains- Oil Red “O” Sudanblack- Best earmine for glyeogen- PAS- Muciearmine, Van Kossa- ZN Stain- Modified Zn stain- Gram’s stain for bacteria- Silver methenamine stain- Prussian Blue stain/reaction- Stain for amyloid

- Basics of immunoperoxides and stain3. Automation in histopathology4. Frozen section technique

Tissue preparationFreezing microtomyMounting of frozen section & staining

5. Basic of Electron MicroscopyClinical pathologySemen AnalysisCSFTheory and abnormalitiesOther body fluid analysisDemonstration of blood parasites.

HAEMATOLOGY

1. Various indices and their brief description2. Absolute Eosinophil count, methods, clinical significance3. Absolute lymphocyte count method significance4. Lab Diagnosis of Haemolytic anaemia

- Definition and types of anaemia- Reticuloecyte count, preparation & stain- Osmotic fragility test- Heinz body demonstration- Demonstration of sickling (Sickle cell anemia)- Diagnosis of enzyme deficiency-G6PD- DCT/ICT- Haemoglobin electrophoresis- Demonstration estimation of HbF

5. Coagulation studies- Basics of coagulation system & coagulation defects

- BT, CT, Clot retraction- PT, PTTK- FDP- Euglobin lysis test

6. Bones marrow examination - Structure & function of bone marrow- Collection of bone marrow smears- Significance of bones marrow exam- Special stain in bone marrow smears

7. Leukaemia- Theory- Classification

8. Cytochemical stain- Principles of staining & theory- PAS- MPO (Myeloperoxidase)- Sudden Black- Neutrophil alkaline phosphastase (NAP)- Chloroacetate esterase- Non specific esterase

9. Demonstration of malarial Parasite and other blood parasites.10. LE cell test theory, preparation staining & clinical importance11. Benee Jone’s protein12. Theory of electrophoresis13. Automation in heametology

IMMUNOHAEMALTOLOGY & BLOOD BANKING

Theory of blood transfusion reactions.

Investigation in case if Rh incompatibility DCT/ICT

Investigation in case of ABO incompatibility

Preparation & storage of different blood components

- RBC concentrate - Single donor plasma- Cryoprecipitate- Platelet rich plasma, platelet concentrate

Blood Bank audit

Safety, quality, record keeping in Blood Bank

Quality Control

CYTOLOGY

Use of centrifuge & Millipore technique

Demonstration of sex chromatin

Special stain (theory & preparation)

- AFB- Shorr stain- Mucicarmine- PAS- Methylene blue- H&E stain

Characters of benign and malignant cell

Revision of 1st Year syllabus in all subjets

2nd Year Pathology Practicals

HISTOPATHOLOGY : -

1. Handing & processing of small tissue fragments & bone2. Revision of routine H&E stain

3. Preparation of reagents for special stain4. Staining techniques for special staining procedures as per theory

syllabus.5. Use of automatics tissue processor (Histokinette)

CLINICLA PATHOLOGY : -

Urine Examination : -

- Revision of routine examination (R/E)- Test for ketone bodies- Test fir Benee – Jone’s proteins- Test for Bite salts- Haemoglobinuria, myoglobinuria, Haemosiderinuria

M/E of Urine for

- Haematuria- Casts- Pus cells- Abnormal cell – malignant cell

Stool Examination : -

- Revision R/E- Stool for occult blood- Stool for pus cells- Stool for undigested food

Semen Analysis : -

- Specimen collection- Examination

CSF Analysis : -

- Gross examination- M/E- RBC- WBC- Other Cells- Cells count in CSF

Staining for Bacteria

HAEMATOLOGY

- Preparation of reagent/stain for reticuloeyte count- Osmotic fragility- LE cell test- Demonstration of sickle cell- Buffy coat preparation

Special stain

- Preparation of reagents stain- PAS-Sudden Black- MPO-Non specific esterase- NAP- BT- CT

IMMUNOHAEMATOLOGY

Blood screening – for AIDs, Syphilis, Hepatitis & Malaria

- Venipuncture & Blood collection & storage- DCT/ICT

Preparation of Packed cells

- PRP & FFP

SYLLABUS FOR DMLT MICRPBILOGOY (THEORY)

(1st Year)

Topic- 1 health and safety in the laboratory

L-1.1 Biological hazards, level of containment and laboratory design, health of staff. Substance hazards to health, hazard label, safety procedure, Biosafety cabinets, general procedures.

L-1.2 Transports of hazardous substance, container, transport bags, transport within hospital and outside places. Precaution against fire an management of fire. Storage of chemicals.

L-1.3 Segregation of waste and disposal of waste material.

L-1.4 First aid. Maintenance of accident register.

Topic-2 Elementary microscopy.

L-2.1 Principal of the Microscope

L-2.2 Components of Microscope, optical, Filters, micrometry, magnification setting up the microscope.

L-2.3 Different type of microscope

Topic -3 Sample collection and transportation.

L-3.1 Collection of various specimens

L-3.2 Storage and transports to laboratory, receipt of specimen, storage of processed, postal specimen.

L-3.3 Specimen container, Swabs, autopsy and biopsy specimen.

Topic -4 introduction to Microbiology.

L-4.1 Historical survey, classification of micro organism, bacterial micrphology.

L-4.2 Bacterial structure and metabolism, Bacterial variation.

L-4.3 Bacterial associations and pathogenicity.

Topic -5 Quality assurance in microbiology laboratory.

Topic -6 Microscopic examination of Bacteria

L-6.1 Making if Loop and sterilization, making of smears, hanging dro preparation, wet preparations.

L-6.2 Staining of smear grams staining, Ziehl Neelsen staining, Auramin phesol staining.

L-6.3 Simple staings and counter stain, Methylene Blue, malachite green, Loefflers alkaline methyene blue, polychrome methylene blue, Albert stain.

Topic -7 Sterilization

L-7.1 Physical methods, sterility assurance, sterility control, Microbiological testing for sterility.

L-7.2 Control of process of sterilization. Thermocouples, Biological controls.

L-7.3 Chemical method of sterilization.

L-7.4 Sterilization by filteration.

Topic -8 The principal and use of culture media.

L-8.1 Essential requirement of culture media, environmental factors. Gaseous requirement. pH. Temperature, types of media-liquid Solid Enriched, Differential, Selective Enrichment. Auxanographic media, transport media.

L-8.2 Storage of culture media, Shelf life of different media, media identification, Colour coding, beads in liquid media. Coloured cotton plugs.

L-8.3 plate cultured method, Slope cultures, deep cultures and roll tube cultures.

Topic -9 Preparation of culture Media.

L-9.1 Dehydrated culture media, distilled water, deionised water, faults during preparation of dehydrated culture media, adjustment of pH-Colorimetric method, Lovibond comparator, physical balance, electronic balance.

L-9.2 preparation of culture media, notes on commonly used media-nutrient, Broth, Infusion Broth, Digest broth, Blood Broth, meat extract broth, broth, serum broth, Chocolate broth, Field’s Broth.

L-9.3 Glucose Broths, nutrient agar, nutrient gelatin, Carbohydrate, media burharms tube.

L-9.4 Peptone water, peptone water sugars, Hiss serum water sugars, Solids sugar medium, litmus milk Common media used for Biochemical reactions.

L-9.5 media for special purpose-Mac conkey’s agar, Desoxy cholate citrate agar, CLED (cystine-lactose electrolyte deficient medium, Serenity F medium, Phenylalanine agar, Loefflers, Serum Slopes.

L-9.6 Loweistein-jensen medium, Mannitol salt agar, TCBS medium (Thiosuphate, Citrate Bile, salt agar medium) Bordel Gengos medium, Cooked meat mediaum.

L-9.7 transport media-Stuart transport medium, amie’s transport medium, Quality control of media, testing of culture media, Selection of control stain, Sterility testing.

SECOND YEAR

Topic -10 methods of anaerobic cultivation of Bactria.

L-10.1 Anaerobic bacteriology methods of excluding oxygen from liquid or semi solid media, anaerobic jar and cabinet, control of anaerobic jars and cabinets.

L-10.2 Indicators-Alkaline methylene blue-Glucose solution, Semi solid indicators, use of anaerobic jars for Co2 dependent cultures, handling anaerobic micro organism.

Topic -11 Antigen-Antibody reactions.

L-11.1 Antigens and Antibodies, H and O antigens, Agglutination tests, Slide and tube agglutination, carrier particle, agglutination, latex agglutination. Haem agglutination, co agglutination.

L-11.2 Precipition-Lancefield grouping, Gel Precipitation method, complement fixation, labeled antibody tests, Dilutions.

Topic -12 Routine Bacteriological examination of specimens.

L-12.1 General procedures, handing of high risk specimens and general precautions, disposal of pathological specimens.

L-12.2 Examination of specimens, microscopic examination, culture and examination, Microscope appearance of bacterial, blood culture, Automated blood culture.

L-12.3 CSF feaces, Fluids-pleaural, peritoneal & others, Puc, Sputum examination of for micro organism other than M. tuberculosis Swabs-throat swabs, post nasal and pernasal swabs, Genitourinary swabs, eye swabs. Laryngeal swabs.

L-12.4 Giatric lavage, urine-complete processing for micro organisms other than M. tuberculosis.

L-12.5 Commonly isolated, micro organisms from clinical samples- brief introduction only.

L-12.6 Tests for identification of organisms, aeseulin hydrolysis, catabase, coagulate activity, test for indote production, optoenine sensitivity, oxidase test, oxidation fermentation reaction, phenylalanine Deaminase test.

L-12.7 Antimicrobial susceptibility testing-MIC, agar diffusion methods, stokes method, Kirby-technique, antibiotic assay in fluids.

Topic -13 Medical Mycology.

L-13.1 General introduction

L-13.2 brief introduction of fungi causing disease in humans.

L-13.3. Brief introduction to fungi causing opportunistic infections.

L-13.4 Collection and transport of specimen-skin, nails, hairs, sputum, exudates, body fluids and blood microscope methods and slide culture, Unease test. In vitro hair penetration test, pigment production, India ink preparation to demonstrate capsule of yeasts, production of germ tube.

Topic-14 Virology

L-14.1 General introduction and classification.

L-14.2 Collection and transport of specimens.

L-14.3 briefly about various culture methods.

L-14.4 Brief introduction of viruses transmitted through blood and air borne route.

L-14.5 Safety precaution, Serological assays used in virology PCR.

Topic-15 Parasitology

L-15.1 General introduction to parasitology, Historical prospective. Epidemiology, parasite host relationship, parasite life cycle, disease process and symptoms, prevention and control.

L-15.2 Parasite classification-Protozoans and helminthes, Arthropods and their general characters.

L-15.3 Briefly about common protozoan’s

L-15.4 Briefly about Helminthes 1

L-15.5 Briefly about Helminthes 2

L-15.6 Briefly about Arthropods

L-15.7 Sample collection, storage transport and processing

L-15.8 Direct examination for parasites

Macroscopic

Microscopic

L-15.9 Culture of specimens for parasites

L-15.10 Serological methods for parasites.

MICROBILOGY (PRACTICAL)(FIRST YEAR)

P.1 Orientation of microbiology Laboratory and its various sections.

P.2 Common Laboratory Glassware. Preparation, Sterilization and uses.

P.3 Autoclave, functioning, loading and sterilization.

P.4 hot air oven, functioning, loading and sterilization.

P.5 Incubator, setting temperature, standardization of thermometers.

P.6 Sitting up of water bath inspissator.

P.7 Refrigerator-leveling, loading, temperature maintenance and care.

P.8 Deep freezer leveling, loading, temperature maintenance.

P.9 Physical balance : Operation, handling and care.

P.10 Accident register, maintenance in laboratory

P.11 Fire and its management, First aid technique.

P.12 Filling up of requisition forms, collection of samples, labeling and storage.

P.13 VDRL shaker, setting up, checking for RPM.

P.14 Microscope parts maintenance and user.

P.15 Segregation of waste, Disinfection, Discard jars, Disposal.

P.16 Quality control and assurance methods.

P.17 making wire loops of different internal diameters.

Sterilization, hanging loop preparation, wet mounts.

(SECOND YEAR)

P.18 Grams staining –preparation and staining of smears

P.19 Ziehl-Neelsen staining –preparation and staining of AFB smears

P.20 Methylene Blue. Malchite green,Albert and Giemsa staining

P.21 Staining of bacterial spores

P.22 Capsule demonstration technique

P.23 preparation of commonly used bacteriological media like infusion broth, Nutrient broth, Digest broth, meat extract broth, blood broth, Serum broth Flides broth, peptone broth, Alkaline peptone water, selenite F broth.

P.24 preparation of glucose broth, nutrient agar, Blood agar, Chocolate agar, Serum agar, nutrient gelatin, Carbohydrate fermentation media.

P.25 Preparation of MacConkey agar, Desoxycholate agar, Cystine lactose electrolytes deficient medium, Wilson’s and Balairs medium, Phenyl alkaline agar, loefflers serum slope.

P.26 Preparation of LJ medium, m,annitol salt agar, TCBS cooked meat media.

P.27 Preparation of transport media-stuarts, amines.

P.28 Study of common laboratory isolates

P.29 Preparation of various dyes, indicators etc.

P.30 preparation of commonly used media for biochemical reactions.

P.31 Demonstration of precipitation and agglutination reaction

P.32 Centrifuge and its uses in relation of various investigations.

P.33 urine microscopy, Normal and abnormal

P.34 Stool examination, Normal and for process of various ova, cysts and parasites

P.35 Demonstration of various test like catalase test, oxidase test, optochin sensitivity, Phenylalanine deaminase test and indole production.

P.36 Antimicrobial susceptibility by stokes and Kirby Bauer methods, making of antibiotic discs, storage etc.

P.37 Fungus culture, Different commonly used media, inoculation, wet mount, preparation, India ink preparation, germs tube test.

P.38 ELISA test, DOT tests, VDRL test, CFT

P.39 Collection and preservation of stool samples

P.40 processing stool sample for demonstration of parasites, ova & cysts

P.41 Collection of blood to demonstrate Haemoflagellates

P.42 Visit o medical Collage laboratory in nearby area.

SYLLABUS FOR DMLT COURSE

BIOCHEMISTRYTHEORY(1st YEAR)

INSTRUCTION TO MEDICAL LABORATORY TECHNOLOGY

Ethics

Responsibility

Safety measures and first aid

Clean mg and care of general laboratory glassware

Different cleaning agents

Methods of cleaning

Preparation of reagents and standards solutions, buffers

Storage of chemicals

PH and measurement of pH

UNITS OF MEASUREMENTS: -

SI units, measurement of volumes, volumetric apparatus and their calibration

IMPROTANT INSTRUMENTS, PRINCIPLE WORKING AND CARE OF : -

Balance (Analytical, electrical/electronic)

Centrifugation and types of centrifuges

Colorimeter

Spectrophtotometer

Normal and reference range

Basic concept in computers

Collection preservation and recording of biological specimens

Composition of blood and its functions

Use of various antieoagulants

Separation of serum and plasma

Different protein precipitation agents

Preparation of protein free filtrates

Classification, Nomenclature and function of various biomolecules

Amina Acids, protein lipid Carbohydrates, Nuleic Acid and Enzymes

Role of ATP

Normal metabolism of the body

Carbohydrate metabolism

Lipid Metabolism

Protein metabolism

Vitamins and Hormones

PRACTICAL(1st YEAR)

Cleaning of glassware

Sterilization of glassware

Standardization of glass wares

Handling and maintenance of each instrument

Preparation of various anticoagulant and specimen collection bottles

Collection of blood

Separation of serum and plasma

Preparation of different proteins precipitating agents

Protein free filtrate preparation

Qualitative analysis of Carbohydrates, proteins, lipid.

THEORY(2ND YEAR)

Blood Sugar Estimation and GT

Renal threshold

Clinical importance of blood sugar and GTT

Serum Urea

Formation and excretion of Urea

Normal and abnormal levels

Plasma and serum proteins

PRINCIPLE PROCEDURE AND CLINICAL SIGNIGICANCE OF THE ESTIMATION OF : -

Blood Sugar

Serum urea

Plasma and serum proteins

Serum Cholesterol

Serum Electrolytes and trace elements

Na+, K+, CL-, Ca+2, Li+

Bilirubin

Phophorus (Inorganic)

S Urea Acid

S Creatinine

S Calcium

CLINICAL ENZYMOLOGY

SGOT and SGPT

S Alkaline phosphatase

S Acid Phosphatase

Serum Amylase

Normal/Abnormal Constituents of urine

Biochemical composition of CSF and

Clinical significance of each component

Method of determination of sugar, chloride and protein in CSF

Principle and procedure of determination of blood gasses

Principle and procedure of electrophoresis and chromatography

Principle of ELISA

Use of semiautomatic Analyzer and ISE based electrolyte analyzes

Quality assurance

Quality Control

PRACTICALS(2ND YEAR)

PRACTICAL ESTIMATION OF LOWING BYMANNUAL AS WELL AS ENZYMAIC PROCEDURE : -

Blood Sugar

S Urea

Plasma and serum proteins

Serum Cholesterol

Electrolytes and trace elements

Bilirubin

Inorganic phosphorus

S Urie Acid

S Creatinine

S Calcium

SGOT and SGPT

S Alkaline phosphatase

S Acid Phosphatase

Serum Amylase

S Aluminum

S Protein (Total)

GGT

LDH

CPK

SPECIAL INVESTIGATIONS

LIPID PROFILE

Hormone assay

Acid base Gas Analysis

CHEMICAL & REAGENTS

All the chemical, reagents, glassware and other miscellaneous items required for conducting the practical mentioned in the syllabus.

FURNITURE/OTHER ITEM LIST FOR INSTIUTION ALL LABS (COMMON)

Executive table 1

Deluxe Chairs (Cushioned) 15

Steel Almirah (large) 4

Steel Almirah (Small) 4

Sofaset OPTIONAL 1

Center table OPTIONAL 1

Office table 3

Executive Chairs (Revolving) 4

Revolving Stool (STEEL) 75

Name Plates As required

Foot operated dustbins 5

Staff position Alphabetical Board 1

Steel rack (3 Shelf) 3

Selves for reagent bottles As required

Examination table 1

Footrest 1

Heat Pillar/Room heater (1500 watts each) 10

Library bookrack 1

Seminar Chairs 25

Black Boards 4

Display Board 1

Laboratory table with granite top, sinks and 5 in each lab.(Water connection (four students on each table) orWorking bench 3 feet width along the wall of labs With sink as water connection tube light each, Electrical point two students/seat i.e 10 seats.)

Lockers for microscope 20

Table for keeping Lab equipments 3 feetx10 Feet 1

Projector 1

Computer with printer 3

One head LCD projector with screen 1

Pointer 4

Notice Board 1

GLASSWARE (COMMON LIST FOR ALL LABS)

Test tubes 12x100mm

15x150mm

12x75mm

Petridis 89mm

Flask flat 50ml

1000ml

2000ml

Reagent 250ml(Transplant and Amber coloured)

Bottles 500ml

1000ml

Dropping 50ml

Bottles 500ml

1000ml

Funnel 75mm

125mm

Slides 26x76mmx1mm

Slides with

Concavities

Cover slip 22x40mm

Pipette graduated class A 0.01ml

1ml

2ml

Pipette graduated class B 1ml

2ml

5ml

10ml

Pipette(one mark Class B) 25ml

Volumetric Beaker 100ml

250ml

500ml

Measuring cylinder 10ml

50ml

100ml

250ml

500ml

1000ml

Dryers tubes

Washermans tubes

Durhams tube

Aluminum rack for 12mm tube

Aluminum rack for 25mm tubes

Pasteur pipette

Screw cap bottle 100ml

Petridish 100x17mm

80x17mm

Tube Culture media 25x57mm 18x45mm

25x95mm

Rubber teats medium

Small

Wash Bottles

Specimens jar 200x125x125ml

Vials with screw caps

Reagent troughs U shape

Coplin jars

Staining jars of troughs

Slide draining racks

Slide carries

EQUIPMENT

PATHOLOGY LABORATORY (SEPARATE)

Manocilar Microscopes/Binocular 20

Haemoglobinomet Sahil’s 20

Haemoglobinometer tubes (hellige square) 20

Haemoglobin pipettes 20

Stirrer 20

Improved Neubar’s counting chamber 20

RBC pipette 20

WBC pipette 20

Wintrobe’s tubes 20

Westergran’s pipettes with stand 20

Slide draining racks 10

Slide carries (folder) 20

Automatic tissue processor 1

Histokinette 5

Jars for manual processing glass 2Ltrs 10

Paraffin wax bath hot plate 1

Incubator jar 2 Ltrs 2

Rotary microtome with blades/knife 2

Tissue flotation bath 2

Hones (Belgium or plate glass) 1

Strop Horse (Leather) 1

Automatic knife sharpener (optional) 1

Bone Cutter (electric) 1

Cassettes for tissue processing 1

Metal racks-tube rack 1

Staining jars or troughs for 10 slides 20

Slide cabinet 5

Storage container for blocks 10

Seissors pointed and blunt tipped 6” 1

Instruments for grossing 1 set

Blunt forceps 3

Arterv forceps 4

Slide warmer 60’C 3

4

1

2 Sets

4

Leuknart’s (L) Blocks 10

Water bath 1

Centrifuge 4000Spm. 12tube 1

Refrigerator 165Ltr 1

Thermometer laboratory 2

Slide Boxes wooden 100 slides capacity 12

Diamond pencils 5

Glass distillation plant 1

Cytocentrifuge (Optional) 10

Disposal blade for microtome 3Low profile (stainless steel)

Weight machine (digital) range 10mg to500gm 2

Perforated bucked for manual processing 6Saw with hexa blade 2

Weight Box and practical weight 1 set

E1 tray 1. 17x20cm 1

2. 25x30cm 1

3. 30x35cm 1

4. 30x45cm 1

Heat convertor (Blowers) 2

Coplin jar for 10 slides 10

Frozen’s syringe holder 2

Spirit lamps 20

Nine unit laboratory cell counter 2

Automated heamatological cell counter 13 parts (i.e.R.B.C., W.B.C. & plated profile)

CHARTSCharts : -

Steps of commonly used stains

Steps of special staining method

Haematopoiesis

Normal haematogical values

Immune System

Steps of PAP staining

Difference between benign and malignant cells

CSF in health and disease

CONSUMABLE ITEMSREQUIREMENT OF HISTOPATHOLOGY/HAEMATHOLOGY (AS

REQUIRED)

1. 37-40% Formaladehyde

2. Acetone

3. Benzene

4. Chloroform

5. Absolute alcohol

6. Xylene

7. Paraffin wax

8. Liquid paraffin

9. Singer Oil

10. Abrasive power (coarse, medium, fine)

11. D.P.X.

12. Haematoxyline powder

13. Ferric Chloride

14. Hydrochloric acid

15. Picric acid

16. Acid fuchsin

17. Potassium iodide

18. Iodine crystals

19. Phosphotungstic acid

20. Potassium permagnate

21. Oxalic acid

22. Potassium ferrocynide

23. Silver nitrate

24. Potassium aluminum sulphate

25. Mercuric oxide

26. Ammonia water 28 percent Liquid ammonia

27. Lithium Carbonate

28. Glycerin

29. Sodium iodate

30. Periodic acid

31. Basic fuchsin

32. Potassium metabisulphite

33. Charcoalactive

34. Light green SF yellowsisn

35. Sulphuric Acid A.R.

36. Ferric ammonium sulphate

37. Sodium hydroxide

38. Uranium nitrate

39. Gold Chloride

40. Sodium thiosulphate

41. Methyl violet

42. Crystal violet

43. Carbolic acid

44. Methylen blue

45. Formic acid

46. Sodium citrate

47. Nitric acid

48. Bismark brown

49. Orange-G 6

50. Ether (element)

51. Malachite green

52. Sudan black, II,II, IV

53. Isoproply alcohol

54. Phloxine

55. Acetic Acid, Glacial

56. Borax

57. Azure B

58. Methyl alcohol

59. Absolute propylene glycol

60. Egg albumin

61. Starch adhesive

62. Calcium carloride

63. Phosphomolybic acid

64. Merecurie chloride

65. Ammonium aluminum sulphate

66. Phosphomolybic acid

67. Aluminum chloride-anhydrous

68. Potassium chloride

69. Potassium carbonate

70. Sodium acetate

71. Chromium tetraoxide

72. Para aldehyde

73. Sodium borate

74. Methanamine

75. Sodium bisulphate

76. Sodium bicarbonate

77. Ferrie Oxide AR

78. Aluminum Oxide AR

79. Alcian blue W/S

80. Oil Red O

81. Scarlet B

82. Congo red

83. Carmine

84. Bicbrish scarlet red

85. Metanit yellow

86. Citric aid

87. Gelatin

88. Copper sulphate anhydrous

89. Tannic acid

90. Chloral hydras

91. Thymol

92. Cotton

93. Gauge piece

94. Whatman filter paper one and two

95. Towels

96. Methylene blue

97. Toludine blue

98. Gentian violet

99. ETDA

100. Double oxalate

101. Giemsa Stain

102. Sodium Stain

103. Pot dichromate

ANY OTHER REAGENS AS OER PRESCRIBED SYLLABUS : -

104. Rubber

105. Vim

106. Duster

107. Soap

108. Phenyl

109. Dettol

110. Tissue paper

111. Sodium hypochioride

112. Brushes for cleaning glassware : -

1. Small 10

2. large 10

3. Tilted brush10

113. EA 50 & 36

114. Aluminum hydroxide Anhydraw

115. fire extinguishers (every lab)

a. Paper/wood fire

b. Chemical/gas fire

c. Electrical fire

LIST OF REQUIREMENT FOR DMLT COURSE IN PARAMEDICAL INSTITUION IN HP MICROBILOGY LABORATORY: -

ITEM Quantity Required

1. Hot air Oven 24”x24”x24” 1

2. Incubator 18”x18”x18” 1

3. Test tude holder 20

4. Slide Boxes, Capacity 100 10

5. Aluminum slide trays 10

6. Forceps toothed 20

7. Loop (changeable wire) 20

8. Loop 9Fixed nichrome wire) 20

9. Loop holder with straight wire 20

10. Diamond pencil 3

11. Bunsen burner 10

12. High pressure tubing for gas 8mm 40 meters

13. High pressure tubing for gas 10mm 40 meters

14. Animal balance (5 KG) 1

15. Tissue grinder 2

16. Mc intosh and Flides anacrobic jar 2

17. Binocular microscope 3

18. Trolly with 4 wheels for transporting material 1

19. Colour coded container for waste segregation set of

4 in each lab

20. Discard jars with lids 10

21. Safety hood inoculation chamber 2

22. pH meter 2

23. Autoclave vertical 450mmx250mm 1

24. Shaking water bath 1

25. Monocular microscopes 10

26. Centrifuge 5000RPM 2

27. Angular head (15mlx8) 2

28. Angular head (50mlx4) 2

29. Wooden cabinets for microscopes 10

30. Autoclave vertical 550x350mm 1

31. Spirit lamp brass 10

32. Electrical needle destroyer 1

33. Manual needle destroyer 1

34. Serological water bath 14”x12”x7” 2

35. Double distillation plant (all glass) 1

36. Vortex mixers (with head for plate and tube) 1

37. Electronic timer 1

38. Magnetic stirrer with hot plate 1

39. Type write godrej 47CMS 1

40. Phate ELISA reader and washer (any make) 1

41. Overhead projector 1

42. Serum inspissator 1

43. Hot plate 8” (round Shape) 1

44. Hot plate rectangular 1

45. Magnifying lens 10X 2 Dozens

46. Ultra violet lamp/Tube 1

47. Eye piece 10X 6Nos

48. Refrigerator 165 Ltrs 1

49. Refrigerator 300 Ltrs 1

50. Gas cylinder and regulator and pipe LPG 2 sets

51. Vortex mixers 1

52. Electronic timer 1

53. Magnetic stirrer with Hot plate 1

54. Serum inspissator 1

55. Trolly with 4 wheels for transporting material 100 mtrs 1

56. Discard jar with lids 6

CONSUMBLE ITEMS (MICROBIOLOGY LABORATORY) AS REQUIRED :-

NOTE : - Should be made available to students regular for practical sessions and replenished as and when required. Additional items may be procured if felt necessary by the teaching faculty, to complete the practical exercise.

1. ANTIBIOTIC DISCS Vialsa) Streptomycinb) Chloramphrenicolc) Tetracyclined) Erythromycine) Furadantinf) Penicilling) Kanamycinh) Gentamycin

2. Widal test kit3. Acids

a) Lacticb) Acetic acid (Glacial)c) Cone. Nitric Acid ARd) Cone . Hydrochloride Acid ARe) Ascorbic acidf) Citric acidg) Tartaric acid

4. Amino acid kit5. VDRI kit

STAINS AND INDICATORS : -

a) Gentian violetb) Saffraninc) Basic fuchsind) Acid fuchsine) Malachite greenf) Mathylene blueg) Methyl redh) Neutral redi) Bromothymol bluej) Bromoeresol purplek) Phenolphathelinel) Brilliant greenm)Bromophenol bluen) Crystal violeto) Methyl violetp) Indian ink

ORGANIC SOLVENTS

a) Ethyl alcoholb) Acetonec) Chloroformd) Ether e) Phenol (crystals)f) Thymolg) Cresol with soap solutionh) Glycerini) Methyl alcohol (methanol)

ORGNIC REAGENTS

a) Glycerol mono acetate

b) P-Dimethylamin benzaldehyde

c) Tetra methyl para pheneylene

d) Diamine dihydhochloride –Naphthlo

e) Tween 80(polyoxyethylene Sorbitiiol monooleate)

f) Starch

g) Creatine

h) Urea

i) Optochin

INORGANIC SALTS

1) Sodium chloride

2) Sodium hydroxide

3) Sodium sulphate

4) Na-citrate

5) Na-pyruvate

6) Na-deoxycholate

7) Na-OHPO4

8) Na-HPO4

9) Na acid selenite

10) Na-taurocholate

11) Na2CO3

12) NaHCO3

13) Na-anacalate

14) Na-phiosulphate

15) Na2SO4

16) NaNO3

17) Resaturin Sodium

18) Na-glycerolphophate

19) Potassium dichromate

20) NK Tantenate

21) K-1

22) K2HPO4

23) KQN

24) K-tellurite

25) Bismuth Sulphite

26) Iodine

27) Ferrie ammonium citrate

28) FeCI

29) FeSO4

30) CaCI2

31) CaCo3

32) MgSO4

33) Mg-Citrate

34) CuSO4

35) MnSO4

36) NH4OH(conc.)

37) (NH4)2HPO

38) (NH4)2H2PO4

39) (NH4)2SO4

40) (NH4)

41) Bismath ammonium citrate

42) Glucose

43) Glucose 6-Phophate

44) Galactose

45) Mannose

46) Maltose

47) Manritol

48) Arabinose

49) Xylose

50) Fructose

51) Inulin

52) Lactose

53) Sucrose

54) Agar agar

55) Liver extract

56) Egg

57) Meat (according the requirement)

58) Serum (commercially prepared)

59) Pancreatic extract

60) Yeas

61) H2O2

62) ZnSO2

63) KOH

64) Sheep or arrangement to obtain sheep blood from slaughter

house

CONSUMABLE ITEMS (MICROBILOGY)

1) Wire gauze2) Plasticin3) Sticking plaster (leucoplast)4) Gum paper5) Cotton wood absorbent6) Cotton wool non absorbent7) Filter paper8) Whatman filter peper

9) Coarse filter paper10) pH paper (unviersal range)11) Rubber gloves (heavy Duty)12) Surf13) Vim14) Duster15) Lifebuoy soap16) Dettol17) Towels18) Thread19) Glass making pencil’20) VDRL (syringe)

MISCELLANEOU (AS REQUIRED)

1) Pipette stands2) Paraffin ring maker3) Pipette holder4) Spatula 5) Dessicator6) Perforated aluminum basket (medium)7) Filter stand8) Enamel tray without lid9) Persplex covers for microscope

BIOCHEMISTRY LABORATORY (SEPARATE)

1) Elisa reader with washer 12) Semiautoanalyser 23) Analytic balance 14) Physical balance 15) Coagulometer 16) Urine strip As required7) Albuminometer 18) Reflux condenser 19) Burrettes 2010) Folin Wu tubes 2011) Spacaulas 1212) Centrifuge (5000RPM) brushless 213) Water baths 37”C 1

14) Water bath boiling 115) Hot air oven 116) Spectrophotometer 117) pH meter 118) All glass distillation apparatus 119) Micro pipetters variable Vol.5-50ul 520) Auto pipettes fixed Vol. 521) Micro pipette variable Vol.200-1000ul 122) Inculator 123) Beaker 24) Cylenders(50ml,100ml,250ml,500ml & 1000ml) 2Each25) Test tubes26) Flasks 5