Ultra-fastPLATINblue2 UHPLC from KNAUER

Fast

PLATINblue UHPLC�ultimate�performance�HPLC�with�an�extended�pressure�range�up�to�1000�bar�(15000�psi)�at�fl�ow�rates�<�1.5�ml/min�

�minimal�dead�volume�of�25�µl�with�SmartMix�gradient�mixer

2

2

Short cycle timefast�gradients

�high-performance�autosampler�for�fast�injection�(15�s�or�<��0�s�with�needle�wash)

�optimized�communication�and�software�operations�for�short�initialization�times

2

2

2

High throughput�process�up�to�7���samples�in�2�x�3�4-well�plates�or�up�to����samples�in�standard�1.��ml�vials

2

Speed and productivity

Fast detection�data�acquisition�rates�up�to�200�Hz�provide�higher�resolution�and�peak�capacity,�as�well�as�a�more�accurate�integration

2

Increase your laboratory’s productivity by taking advantage of the benefi ts of PLATINblue ultra high-performance LC (UHPLC).

With�PLATINblue�UHPLC,�you�can�now�use��conventional�HPLC�columns�as�well�as�columns�packed�with�sub-2�µm�particles�on�one�system�to�achieve:

�faster�separations,�thanks�to�the�use�of�shorter�columns�with�equal�or�higher�separation�performance

�higher�resolution�and�unmatched�separation�effi�ciency�at�equivalent�column�lengths

2

2

2

mA

Um

AU

00

minmin

00

5050

100100

150150

200200

250250

300300

350350

Separation�of�benzoic�acid�esters

Comparison of UHPLC and HPLC

mA

Um

AU

0.960.96

minmin

0.980.98 113838

4040

4242

4444

4646

4848

5050

80 Hz80 Hz50 Hz50 Hz

20 Hz20 Hz

5 Hz5 Hz

Effect of data acquisition rate on the detection of a fast eluting peak

Enlarged�detail,�chromatogram�overlay

3

! TipThe PLATINblue system includes eluent fi lters for protection against particulate matter. When working with columns packed with sub-2 µm particle sizes, samples should also always be fi ltered through a 0.2 µm membrane.

PreciseHigh-quality PLATINblue components were designed to work together perfectly to enable accurate measurement with optimal reproducibility – a prerequisite for analysis results you can rely on.

The sharp and narrow peaks produced by fast chromatography in conjunction with low-noise and low-drift detectors ensure sensitive measurements.

Sensitivity and reproducibility

Ultimate detection sensitivity�electronically�controlled�pump�drive�provides�for�low�pulsation�eluent�fl�ow�and�a�stable�baseline

�high�light�intensity�over�the�full�UV-Vis�range�through�the�combination�of�a�long-life�“high�brightness”�D2�with�a�tungsten-halogen�lamp

�extremely�low�noise�(5�µAU)�and�drift�(<�50�µAU/h)�specifi�cations

�excellent�linearity�of�>�3�AU�for�precise�simultaneous�determination�of�main�substances�and�trace�components

2

2

2

2

4

!TipA very high injection peak can be disadvantageous in fast chromato-graphy since the fi rst sample peaks elute very quickly after the injection. Dissolve the sample in eluent whenever possible to minimize this disturbance.

pea

k ar

ep

eak

areaa

0.010.01

concentration [ng/µlconcentration [ng/µl]]

0.10.1 1110010000

1000010000

100000100000

1010 100100

10000010000000

Linearity of detection over 3 orders of magnitude

anthracene�in�ACN/H2O

ReproducibilityPrecision�can�only�be�as�good�as�the�weakest�link�in�the�chain.�All�critical�components�of�the�PLATINblue�system�were�optimized�for�the�demanding�requirements�of�UHPLC,�resulting�in�a�system�with�excellent�reproducibility.

Optimized:Pump� ✓Eluent�mixing� ✓Injection� ✓Column� ✓Detection� ✓Data�processing� ✓

5

mA

Um

AU

0.00.0

minmin

0.50.5 1.01.000

1010

2020

3030

4040

5050

6060

1.51.5 2.02.0

7070

8080

Six�successive�separations�using�online�mixing�of�a�ternary�eluentEluent:�3�%�CH3COOH/MeOH/ACN�=�7�/20/2Flow�rate:�1�ml/min,�Temp.:�30�°C,�Wavelength:�225�nm

Retention time<�0.3�%�RSD�Peak areas<�1.1�%�RSD�with�partial-loop�injection�(<�0.3�%�RSD�with�full-loop�injection)

Excellent reproducibility of overall system

Easy to useSimple to operate and to service

So that you can stay focused on what is really important, the PLATINblue ultra high-performance LC system is equipped with many useful features that make operation more convenient, relieve you from time-consuming routine checks and assist you in your daily work.

Simple lamp replacement thanks to detachable side panel

�

Comfortable operation�easy�to�read�color�LCD�display

intuitive�touchscreen�control*

�automatic�confi�guration�of�communication�with�PC�and�software�eliminates�time-consuming�manual�setup

�automatic�selection�of�optimal�detector�integration�time

2

2

2

2

TipThe lifespan of the detector lamps can be conserved by having one or both turned off during interruptions in analysis and switched back on automatically when resuming measurements.!

Easy access for quick maintenance

�solid�glass�doors�provide�convenient�access�to�system�components

�removable�side�panel�for�easy�lamp�exchange�and�maintenance�

Reliable and GLP compliant fast�Ethernet�communication

automatic�pump�head�detection�(RFID)

automatic�lamp�detection

automatic�wavelength�verifi�cation

comprehensive�GLP�reporting�functions

Safety and protection�system�leak�sensor,�minimum�pressure�shut-off�and�eluent�level�control�feature�in�ChromGate®�software�provide�protection�against�leaks�and�running�dry

�soft-start�fl�ow�for�protection�of�columns�and�system�components

*�touchscreen�version�optional

2

2

2

2

2

2

2

2

2

7

Intelligent

Smart product features that save time

�automatic�system�configuration�for�the��PLATINblue�system�via�the�instrument�network

navigation�with�tree�structure�for�quick�orientation

Only�ONE�software�for�your�chromatography�laboratory�is�required�to�control�all�KNAUER�instruments�and�a��wide�range�of�chromatography�instrumentation�from�other�manufacturers.�Your�lab�benefits�from�reduced�training�expenses,�uniform�operability�and�harmonized�data�reporting.�

Flexible reporting functions �report�layouts�are�easily�customized�via�multiple�options�in�Custom�Report�feature

�predefined�standard�reports�for�quick�output�of�results

2

2

2

2

User-friendly, scalable and compliant

Automatic configuration of PLATINblue systems makes installation simple

Scalability and compliance

�scalable�from�a�single�instrument��to�a�multi-user�enterprise-wide��solution�thanks�to�Client/Server��functionality

FDA�21�CFR�Part�11�compliance

Easy to use�simple�method�creation�via�software��“wizards”

�“sequence�wizard”�provides�assistance��and�saves�time�when�programming��a�sequence�of�analyses

Convenient diode-array features

clear�presentation�of�3-D�data

�easy�creation�of�spectrum�libraries��and�fast�spectrum�search

2

2

2

2

2

2

ChromGate® PLATINblue Edition is our easy-to-use software solution for laboratories with high demands for functionality, flexibility and scalability, as well as GLP compliance. Based on EZChrom Elite™ software, ChromGate® PLATINblue Edition software adds extended KNAUER-specific functions.

�

�

PLATINblue

2 PLATINblue P-1 Ultra High Pressure Pump

Ultra�high�pressure�pump�optimized�for�fast�liquid�chromatography,�capable�of�pressures�up�to�1000�bar�(15000�psi)�at�fl�ow�rates�up�to�1.5�ml/min.�The�electronically�controlled�pump�drive�provides�for�low�pulsation,�a�highly�precise�fl�ow�rate�and�a�stable�baseline.�

2 PLATINblue M-1 Modular Eluent Manager

Combination�module�to�add�quaternary�gradient�functionality�and�an�online�degasser�for�four�eluents�to�the�ultra�high�pressure�pump.�Additionally,�the�M-1�can�be�equipped�with�an�A/D-D/A�interface�for�adding�devices�with�an�analog�input/output.

2 PLATINblue UV-1 Multiple Wavelength Detector

High�sensitivity�detector�for�data�acquisition�rates�up�to�200�Hz�with�dual-lamp�functionality�for�a�wavelength�range�of�1�0–�00�nm.�Allows�simultaneous�measurement�of�up�to���wavelengths.�Best�in�class�for�noise�and�drift�values.�With�an�unsurpassed�linearity�of�3�AU,�trace�components�can�be�determined�accurately�even�when�main�components�are�present.

10

Component overview

2 PLATINblue T-1 Column Temperature Manager*

This�contact�oven�provides�for�accurate�thermostatting�over�the�entire�length�of�up�to�4�columns�from�10�to�150�°C.�The�eluent�is�equilibrated�before�reaching�the�column�and�is�cooled�before�reaching�the�detector�to�minimize�noise�and�drift.��A�stable�column�temperature�not�only�improves�reproducibility�but�considerably�improves�the�resolution�of�many�HPLC�methods�as�well.

*�ask�for�availability

2 PLATINblue AS-1 Autosampler

Fast�and�extremely�accurate�UHPLC�autosampler�can�handle�pressures�up�to�1000�bar�(15000�psi)�for�injection�of�samples�from�well�plates�and�standard�vials.�Supports�full�and�partial-loop�fi�lling�as�well�as�“microliter-pickup”�for�fl�exible�selection�of�injection�volumes.�The�tray�cooling�option�allows�for�analysis�of�temperature-sensitive�samples.�

2 PLATINblue PDA-1 Diode Array Detector*

This�extremely�sensitive�diode�array�detector�with�a�data�acquisition�rate�up�to�100�Hz�with�dual-lamp�confi�guration�has�a�wavelength�range�of�1�0–�00�nm.�Use�spectrum�data�to�determine�peak�purity�or�to�facilitate�the�identifi�ca-tion�of�unknown�substances.

11

TipPLATINblue components are available with either a touchscreen display or with a small status display for operation with software. Both types can be freely combined in one system.

!

VersatileWide range of application areas

PharmaceuticalDetermination�of�paracetamol�and�typical�impurities�produced�during�synthesis�

�1�� 4-aminophenol� 2�� paracetamol�3�� N-(4-hydroxyphenyl)propanamide�4�� N-(2-hydroxyphenyl)acetamide�5�� N-(3-chloro-4-hydroxyphenyl)acetamide���� 1-(4-hydroxyphenyl)ethanoneoxime�7�� N-phenylacetamide���� 4-(acetylamino)phenyl�acetate���� 4-nitrophenol�10�� chloroacetanilide�11�� 1-(2-hydroxyphenyl)ethanone

PLATINblue systems can be flexibly used to increase the throughput and sensitivity of many applications. Benefit from the advantages of high-speed UHPLC applications without having to completely convert your established HPLC methods. The following are only a few examples of the applications possible with PLATINblue.

EnvironmentalSeparation�of�benzene�derivatives

FoodQuick�determination�of�tocopherols

400

200

0

mA

U

0 1 2 3 4 5min

1

2

345 678 91011

Separation columnBlueOrchid�C1��1.��µm100�x�2��mm

Separation conditions Eluent:�� A:�phosphate�� � buffer�� � B:�ACNFlow�rate:���50�µl/minMode:�� gradientPressure:�� ��50�bar�

(13�00�psi)Detection:��254�nm�� � (�0�Hz,�0.005�s)Temp.:� 50�°C

1�� thiourea2�� toluene3�� propylbenzene4�� butylbenzene

0

mA

U

min

12

3

4

50

100

150

0 1 2 3

Separation columnBlueOrchid�C1��1.��µm100�x�2�mm

Separation conditionsEluent:�� ACN/H2O�� � �5:15�(v/v)Flow�rate:��1000�µl/minPressure:�� 720�bar��� � (10400�psi)Detection:��254�nm��� � (�0�Hz,�0.005�s)Temp.:� 35�°C

1� trans-retinol2�� alpha-tocopherol3�� beta-tocopherol4�� gamma-tocopherol5�� delta-tocopherol��� vitamin�D2

mA

U

0

min

1

2

34

0

5

610

20

30

40

1 2 3 4 5

Separation columnBlueOrchid�Si�1.��µm�0�x�2�mm

Separation conditions Eluent:�� � �heptane/

butanol�1000:4�(v/v)

Flow�rate:��1700�µl/minPressure:�� �350�bar��

(50�0�psi)Detection:��2�0�nm��� � (50�Hz,�0.01s)Temp.:� 25�°C

12

Clinical diagnosticsDetermination�of�sedatives,�e.g.�barbiturates

Selectivity testEngelhardt�test�in�less�than���minutes��

1�� Barbital2�� Luminal3�� Prominal4�� Revonal

mA

U

min

12

34

0 1 2 3 4

0

20

40

60

Separation columnBlueOrchid�C1��1.��µm�0�x�2�mm

Separation conditions Eluent:�� �MeOH/H2O�

�0:40�(v/v)Flow�rate:��500�µl/minPressure:�� ��2��bar�

(11��0�psi)Detection:���254�nm�

(�0�Hz,�0.005�s)Temp.:� 40�°C

1�� uracil2�� aniline3�� phenol4�� p-ethylaniline5�� N,N-dimethylaniline��� benzoic�acid�ethyl�ester7�� toluene��� ethylbenzene

Separation columnBlueOrchid�C1��1.��µm100�x�2�mm

Separation conditions Eluent:� �MeOH/H2O�

49:51�(v/v)Flow�rate:��430�µl/minPressure:�� ��0�bar�� � (12�00�psi)Detection:���254�nm�

(�0�Hz,�0.005s)Temp.:� 40�°C

mA

U

0 2 4 6 8 10min

123

4

5 67

8

25

0

50

75

100

13

Specifi cally designed for ultra high-performance liquid chroma-tography applications, KNAUER UHPLC columns packed with 2 µm or sub-2 µm particles make high-resolution separations possible. Their exceptional stability in both acidic and alkalic eluents make them universally applicable.

Bluespher columns�packed�with�2�µm�stationary�phase�share�many�of�the�physical�properties�of�columns�packed�with�our�long-established�Eurospher�columns�and�facilitate�therefore�the�scaling�of�existing�applications.

BlueOrchid columns�are�packed�with�an�ultra-pure�silica�gel�with�1.��µm�particles�for�ultimate�resolution.�These�columns�exhibit�a�particularly�low�backpressure.

Effi cientKNAUER UHPLC columns

FactsP-1 Ultra High Pressure Pump

Flow rate range� �0.01–3.00�ml/min,�� � adjustable�in�0.001�ml�� � incrementsMax. pressure� �1000�bar�(15000�psi)�at��

<�1.5�ml/min,��00�bar��(�700�psi)�at�<�3.0�ml/min

Flow rate accuracy� ±�1%Flow rate precision � <�0.1�%�RSDHigh-pressure gradient � up�to�4�pumpsLow-pressure gradient up�to�4�eluentsUltra-efficient mixing� yes,�SmartMix�included

M-1 Modular Eluent Manager

Low-pressure mixing/control�of�4�eluentsgradient moduleDegasser module� 4�channels,�Teflon®�AF,��� � high�efficiencyA/D-D/A interface 24-bitLAN� �T10/100�Ethernet�router�

included

UV-1 Multiple Wavelength Detector

Wavelength range 1�0–�00�nm�(UV-Vis)Wavelength accuracy*� <�1�nmLamps� “high-brightness”�D2�and� � tungsten-halogenMax. data acquisition rate 200�HzChannels� max.��Noise*� ±5�µAUDrift*� <�50�µAU/hLinearity*� >�3�AU

*�according�to�ASTM

T-1 Column Temperature Manager

Temperature range� 10–150�°CTemperature stability� +/–�0.1�°CEluent preheating� equilibration�to�temperature� � of�column�compartmentPostcolumn cooling� yesMaximum heating rate ��°C/min

Specifications – PLATINblue

Pump P-1 High-quality materials – stainless steel and glass

Easy access of detector flow cell

14

PDA-1 Photo Diode Array Detector

Wavelength range� 1�0–�00�nm�(UV-Vis)Lamps� “high-brightness”�D2�and��� � tungsten-halogenMax. data acquisition rate 100�HzChannels� �Diodes� 512Noise*� ±5�µAUDrift*� 500�µAU/h

*�according�to�ASTM

15

AS-1 Autosampler

Ultra high pressure up�to�1000�bar�(15000�psi)injectionSample capacity� �max.�7���positions�with��

2�x�3�4-well�plates�or�����standard�1.��ml�vials

Injection cycle time� �15�s,�� � <��0�s�with�needle�wash��Injection modes� �full-loop,�partial-loop�and�

“microliter-pickup”Precision� <�0.3�%�RSD�full-loop�� � injectionCarryover� ��<�0.05�%�with�needle�washSample tray cooling � 4–22�°C

Tech

nica

l�dat

a�ar

e�su

bjec

t�to

�cha

nge�

with

out�

notic

e.

Pump P-1 with SmartMix mixer and pump head

Rear viewTubing guide

Support

www.knauer.net

HPLC · SMB · Osmometry

Wissenschaftliche�GerätebauDr.�Ing.�Herbert�Knauer�GmbHHegauer�Weg�3�141�3�Berlin,�Germany

Phone:�� +4�-(0)30-�0�727-0Telefax:� +4�-(0)30-�015010E-Mail:� info@knauer.netInternet:�www.knauer.net

Fast and reliable

Excellent support starts at the product development stage. That’s why we developed PLATINblue instruments with ultimate reliability in mind. Each component was designed for maximum dependabi-lity and durability, using only the best materials available.

To keep all of the components in your PLATINblue system running with the highest performance, we offer a comprehensive support and maintenance program to ensure your system’s reliable operation.

All support requests will be answered within�two�hours.�Guaranteed!�If�a�problem�arises,�your�personal�PLATINblue�contact�person�will�contact�you�within�24�hours�with�a�detailed�plan�for�resolution�of�the�problem.�KNAUER�management�is�also�at�your�disposal�and�can�be�contacted�by�telephone�when�necessary.�If�so�required,�we�will�solve�the�problem�at�your�site�–�quickly�and�without�complication.�

Our warranty for�PLATINblue�products�has�been�extended�to�three�years.�In�conjunction�with�a�service�agreement,�you�are�entitled�to�an�all-inclusive�full�service�plan.�The�reliable�and�continuous�operation�of�your�PLATINblue�system�is�our�highest�priority.

So you can get off running right from the start,�we�combine�the�PLATINblue�system�installation�with�training�for�you�and�your�team.�A�follow-up�training�session�within�the�following�six�months�is�included�in�the�service�package�at�no�extra�cost.

We will accompany you when�working�out�your�methods,�step�by�step.�Our�application�experts�are�available�Monday�through�Friday�to�assist�you�with�any�questions�you�might�have.

3 Year

24 hService

Con

sult

ing

Installation & Training

Warranty3 Year

24 hService

Con

sult

ing

Installation & Training

Warranty

3 Year

24 hService

Con

sult

ing

Installation & Training

Warranty

3 Year

24 hService

Con

sult

ing

Installation & Training

Warranty

©�KNAUER�April�200�