hptlc and hptlc/ms studies on some bioactive natural products · design & engineering computer...
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HPTLC and HPTLC/MS studies on some
Bioactive Natural ProductsInternational Symposium for Thin-Layer Chromatography
06-08 July 2011BASEL, Switzerland
Dr. J. Madhusudana RaoDirector Grade Scientist & Head, Organic Chemistry Division-1
Indian Institute of Chemical Technology Hyderabad-500607, India
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LAB SCALE – PILOT SCALE – DESIGNS FOR COMMERCIAL PLANT
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SCIENTISTS250
RESEARCHSCHOLARS
750
RA/QRSFELLOWS
25TECHNICAL
145
TECHNICIANS169
AUXTECHNICIANS
259
ADMN STAFF133
CONTRACTWORKERS
444
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MANAGEMENTCOUNCIL DIRECTOR RESEARCH
COUNCIL
ADMINISTRATION
FINANCE
STORES AND PURCHASE
INORGANIC &PHYSICAL
CHEMISTRY
BUSINESSHRDRESEARCHMANAGEMENT
LIPID SCIENCE &TECHNOLOGY
CHEMICAL ENGINEERING
SCIENCES
BIOLOGYORGANIC COATINGS & POLYMERS
MECHANICALDESIGN &
ENGINEERING
COMPUTER CENTRE
GENERAL ENGINEERING
COAL,GAS ANDENERGY
CHEMICAL &INSTRUMENTAL
ANALYSIS
ORGANIZATION
PHARMACOLOGY
FLUOROORGANICS
PILOT PLANTS
INSTRUMENTATIONLIBRARY &
DOCUMENTATION(IMA)
BIO ENGINEERING& ENVIRONMENTAL
CENTRE
QUALITY MANAGEMENT CHEMICAL
BIOLOGY
NATURAL PRODUCTS
AGROCHEMICALS
DRUGS &INTERMEDIATES
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Natural Products Division (Organic Division-1)
1. Natural Products2. Synthetic Chemistry3. Molecular Modeling4. Pheromones 5. Chemical Biology
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NATURAL PRODUCTS
1. NEW BIOACTIVE MOLECULES FOR DRUGSAND PESTICIDAL APPLICATIONS
2. LEAD OPTIMIZATION USING COMBINATORIALNATURAL PRODUCTS AND ANALOGUE BASED APPROACH
3. HERBAL DRUGS AND THEIR STANDARDIZATION
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Global Herbal drug sales: $100.0 Billion
Developed Countries Share: 50%
Indian Share: Low
HERBAL INDUSTRY
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Traditional Systems of Medicine
• Ayurveda
• Sidha
• Unani
• Folklore Medicine.
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The Three Major Issues with Formulations are
1. Quality
2. Safety
3. Efficacy
The Issues Safety and efficacy are directly dependent on Quality
Efficacy
Quality
Safety
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Herbal Drugs & Standardization
Projects completed:
1. Hamdard’s Herbal Formulation-HRF2. Preparation and Standardization by HPLC and HPTLC of
Amla extract-M/S Arjuna Natural Extracts Ltd., Kerala3. Comparison of Chemical fingerprints of Solanum melongena
and BT Solanum melongena-M/S Mahyco-Mosanto Ltd., Mumbai4. Process and Product Standardization of Classical Ayurvedic
Drugs with Special reference to RheumatismDST/IICT/AVS Project
5. Quality Assurance and Validation of some Ayurvedic Formulations for Life Style related and Gyaenicologicaldisorders :DST/IICT/IMPCL,Mohan
6. Isolation of Licoricidine and Licorisoflavone-A from Lico Rice Extract-M/S Colgate Palmolive
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The Various Methods Known for Standardization include
1.Marker compound based standardization
2. Chemical Fingerprinting.
3. Based on Active ingredients.
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Chemical Standardisation
Most Common Ayurvedic Formulations
1.Choornam
2.Kwatham
3.Ghritam
4. Electuary
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ChoornamPowder form of medicinal plants
Hexane Chloroform* Methanol*
MethanolSoluble*
MethanolInsoluble(% Weight)
*Major compound (Marker)QuantificationFinger printing by HPTLC
& % Weight
Chemical Standardisation
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Marker Compound Based Standardisation:
To standardize the herb based on a particular herb with respect to a Marker compound irrespective of whether the compound is
responsible for the said physiological effect.
Eg., Standardisation of Rheum emodi with respect to Chrysophanol,the major Compound.
O
O
OHOH
Chrysophanol
Ref: J Planar Chromatography-Modern TLC, 2002, 15, 128.
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Fig:1: HPTLC chromatogram of Chrysophanol
Fig:2: HPTLC chromatogram of methanolic extract of R. emodi
O
O
OHOH
O
O
OHOH
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Piper longum is standardised with respect to Dihydropiperlonguminine and pellitorine
O
OO
NH
O
NH
Dihydropiperlonguminine
Pellitorine
Ref; J Planar Chromatography-Modern TLC 2006, 19, 238.
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Pellitorine
Dihydropiperlonguminine
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Hedychium spicatum is standardized with respect to hedychenone
O
O
Hedychenone
J Planar Chromatography-Modern TLC, 20(2007), 73-74.
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•To isolate all the possible constituents and to generatefingerprinting protocols for the same.
•Phytochemical investigation of P. longum led to theisolation of 11 constituents.
•Pipataline, Pellitorine, Sesamine, Brachystamide-B,Guineensine, 5-Hydroxy-7,3’,4’-trimethoxyflavone, 5-Hydroxy-7,4’-dimethoxyflavone,5,4’-Dihydroxy-7-methoxyflavone, Diaeudesmin, Piperine,Dihydropiperlonguminine.
Chemical Fingerprinting
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O
ONH
O
O
O
O
OO
O
O
O
O
NH
O
O
O
NH
MeO
MeO
O
O
OMe
OMeO
ON
OO
ONH
O
Pipataline Pellitorine Sesamin
Brachystamide - B Guineensine
Diaeudesmin Piperine dihydropiperlonguminine
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O
OMe
O
MeO
OH
O
OMe
O
MeO
OH
OMe
O
OH
O
MeO
OH
5-hydroxy-7,4’-dimethoxyflavone 5-hydroxy-7,3’,4’-trimethoxyflavone
5,4’-dihydroxy-7-methoxyflavone
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20μl ~ 20μg
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HPLC assisted chemobiological standardisation of α-glucosidase-IEnzyme inhibitory constituents from Piper longum Linn-An IndianMedicinal plantP.V. Srinivas, A.K. Tiwari, V.U.M. Sarma, V. Anuradha, T. Hari Babu,D. Krishna Rao, I.A. Khan and J. Madhusudana Rao*
Journal of Ethnopharmacology, 108(2006) 445-449
Limits of Detection : 0.005-0.001 μg/ml 1-5 ng/ml
Limits of quantification: 0.01-0.05 μg/ml 10-50 ng/ml
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Plants & Marker Compounds Selected
• Piper longum-• Piper nigrum• Piper chaba
DihydropiperlongumininePiperineGuineensinePellitorineTrichostachine
4. Chamundy’s Herbal Formulation for Migrane
1. Piper nigrum - Piperine2. Nicotina tobacum- Nicotine3. Moringa olefera - Tryptophan
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100ng
100ng
500ng
500ng 500ng
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28Hptlc profile of methanolic extracts of piper species
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Detection Limits in HPTLC
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HPTLC/MS study of 4.5-Dihydropiperlonguminine [ 50-1000ng/track].
O
ONH
O
M+ 275, 200ng
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O
O
O
NH
HPTLC/MS study of Guineensine[ 50-1000ng/track].
M+ 383, 50ng
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O
ON
O
HPTLC/MS Study of Piperine[ 50-1000ng/track].
M+ 285, 50ng
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NH
O
HPTLC/MS Study of Pellitorine[ 50-1000ng/track].
M+ 223, 50ng
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N
O
O
O
HPTLC/MS Study of trichostachine [ 50-1000ng/track].
M+ 271, 50ng
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Overlaid chromatograms of individual standards ( track 1-6) with that of methanolic extracts of Piper longum,(track 8-10) Piper chaba(track 11-13)
and Piper nigrum(track 14-16).
Track-6 4, 5-Dihydropiperlongamunine Track-7 Standard mix.
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Overlaid chromatograms of individual standards ( track 1-6) with that of methanolic extracts of Piper longum,(track 8-10) Piper chaba(track 11-13)
and Piper nigrum(track 14-16).
Track-1 Trichostachine. Track-2 Piperine. Track-7 Standard mix.
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Overlaid chromatograms of individual standards ( track 1-6) with that of methanolic extracts of Piper longum,(track 8-10) Piper chaba(track 11-13)
and Piper nigrum(track 14-16).
Track-3 Pellitorine. Track- 4 Guineesine. Track-7 Standard mix.
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Chamundy’s Herbal Formulation for MigraneDetection limits of Marker Compounds
O
ON
O
N
NCH3
H
N
OH
NH2
O
1. Piper nigrumPiperine - 50ng
2.Nicotina tobacumNicotine – 500ng
3. Moringa oleiferaTryptophan – 500ng
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12 3 4
1. Piperine - 50ng2. Piper nigrum extract - Piperine detected3. Chamundy’s Formulation-1 – Piperine not detected4. Chamundy’s Formulation-2 - Piperine not detected
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1 2 34
1. Nicotine - 500ng2. Nicotina tobacum extract – Nicotine detected3. Chamundy’s Formulation-1-Nicotine detected4. Chamundy’s Formulation-2-Nicotine detected
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12
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1. Tryptophan – 500ng2. Moringa oleifera extract – Tryptophan detected3. Chamundy’s Formulation – Tryptophan detected4. Chamundy’s Formulation - Tryptophan detected
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Conclusion
1. Piperine, Pellitorine, Dihydropiperlonguminine could be detectedin Piper Extracts with the HPTLC detection limits
2. Tryptophan and Nicotine could be detected both in individualextracts and Chamundy’s formulations.Piperine could be detected in the individual extract but not inChamundy’s formulations
Future work:
Efforts are under way for determining the Quantification Limits and improve the sample preparation method
for detection Limits using HPTLC/MS
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STANDARDIZATION
1. HPTLC, AMD & DOCUMENTATION SYSTEM
2. HPTLC/MS
3. TLC-FID
THE FACILITIES
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