Calcium Chloride / Gentamicin / Glucose / Magnesium Sulfate /Potassium Chloride / Potassium Phosphate / Sodium Bicarbonate /Sodium Chloride / Sodium Lactate / Sodium Pyruvate

m-HTF : HEPES

HTF medium /m-HTF medium

HTF medium○

○ No animal derivative is contained.

Kitazato HTF medium is suitable to use in multi-gas incubator.For washing sperm, insemination and culture of earlyembryos.

○ m-HTF mediumKitazato m-HTF medium is suitable to use in ambient atmosphere. For handling and manipulation of oocytes/embryos.

HTF medium / m-HTF medium

COMPONENTS

with Human Serum AlbuminHTFS-100

HTFMS-100100mL

Code Details

93441

93443

Order Number Contents

Sterile Filtration / Sterility Test / pH 7.2-7.6 / Osmolality 270-295mOsm/kg / Endotoxin <0.25EU/mL /Mouse Embryo Assay ≥80% / Mouse Oocyte Survival Test ≥80%

Storage: 2-8℃ / Shelf life: HTF= 90 days, m-HTF=180days

QUALITY CONTROL

Cell Culture

Enlargement

CONTACT : Kitazato Corporation International Sales Division1-1-8 Shibadaimon, Minato-ku, Tokyo 105-0012 JAPAN

　　　　

+81-3-3434-1653

+81-3-3434-1652

Copyright© Kitazato Corporation All Rights Reserved. | March 11, 2019 Ver. 1

www.kitazato.co.jpURL Mail trading@kitazato.co.jp TEL

FAXSpecification may change without pre-noticefor purpose of product improvement.

Kitazato Logo is trademarked.HEAD OFFICE : 81 Nakajima, Fuji, Shizuoka 416-0907 Japan

TEST RESULTSFo

rmat

ion

rate

(%)

KITAZATO COMPANY A KITAZATO COMPANY AHTF m-HTF

100

50

Form

atio

n ra

te (%

)

50

94.8%

0

91.2%91.6% 90.5%

293320293320

287317287317

147155

142155

147155

100

0

NS NS

142155

Stable pH value in medium is a very important factor for oocytes and embryos. Kitazato Corporation Co., Ltd has improved the composition of HTF medium. In the result, the pH value rise was minimized when the medium was exposed in ambient atmosphere. On the other hand, the value quickly returned to pH7.4 when the medium was trans-ferred back into a multi-gas incubator.

●Maintain stable pH value

Blastocyst formation rate was 94.8%, when 2-Cell mouse embryos (ICR) were cultured in KITAZATO HTF medium for 72 hours. Slightly better result was obtained with KITAZATO HTF medium compared with another brand.

●Mouse embryo assay

HTF

Blastocyst formation rate was 91.2%, when 2-Cell mouse embryos were processed in the m-HTF medium for 30 minutes and then cultured with KSOM medium for 72 hours. Slightly better result was obtained with KITAZATO HTF medium compared to another brand.

m-HTF

(MEA)

Comparison of pH rise underthe ambient atmosphere. (30min.)

pH

time

KITAZATO

(min.)time(min.)

8.00

7.90

7.80

7.70

7.60

7.50

7.40

7.305 10 15 20 25 30

Comparison of pH changeover the time in gas incubation

pH

8.00

7.90

7.80

7.70

7.60

7.50

7.40

7.3030 60 90 120 150 180 210 240 720

KITAZATO-HTFCOMPANY_A-HTFCOMPANY_B-HTF

KITAZATO-HTFCOMPANY_A-HTFCOMPANY_B-HTF

KITAZATO