Human FibroblastsReplicate the in vivo environment with quality fibroblasts

Research using fibroblasts plays an essential role in the understanding of healing, human disease, and drug discovery. Fibroblasts synthesize the extracellular matrix and collagen, the structural framework (stroma) for tissues. Fibroblasts also play a critical role in wound healing. Research shows that dysfunction of fibroblasts often leads to fibrosis. Fibrosis may in turn lead to other disorders such as anemia (1). Research using fibroblasts can also contribute to a better understanding of genetic diseases such as mucolipidosis II (ML-II) (2). These cells have also been used as a screening tool in the discovery of novel antibiotics in the fight against tuberculosis, an often lethal infectious disease (3).

DV Biologics offers fibroblasts derived from normal donors as well as from donors with chronic or genetic diseases (Table 1). These cells can be an invaluable source for your investigational studies. Our product, skin fibroblasts (PI001-F), can be expanded over 40 population doublings (Fig. 1), without losing its morphological characteristics. Immunocytochemical staining also shows that PI001-F cells synthesize collagen I/III as they express fibroblastic markers (Fig. 2). Functional assays of our fibroblasts demonstrate collagen secretion in the extracellular matrix (Fig. 3).

As research moves away from animal models, it is often challenging for researchers to find cellular models that best represent the human physiological settings. DV Biologics offers an expanding product portfolio of unique cell types and tissue-derived products to help meet that challenge. Our team of dedicated scientists is able to customize products to meet your specific research parameters. Our mission is to provide you with the biological tools needed for the innovation of new technology that will one day be used to treat, or prevent, human degenerative disorders and diseases. All of our products are guaranteed and manufactured under ISO 9001:2008 guidelines.

Figure 1. Growth Characteristics of DVBiologics Skin Fibroblasts (PI001-F).A: Growth Curve: Cells were grown inDV Biologics I-GRO media, dissociated,counted, and subcultured (2×103 cells/cm2) every 7-day period. The results indicate that DV Biologics Skin Fibroblasts (PI001-F) proliferate in culture for 8 or more passages, totaling over 40 population doublings. B: Phase Contrast Photomicrograph of passage 2 cells at 10X. PI001-F cells maintain the typical fibroblast morphology.

Figure 2. Immunocytochemical Characterization of DV Biologics Skin Fibroblasts (PI001-F). Sub-confluent PI001-F culturewas fixed, and labeled with anti-fibroblast (green) and anti-Collagen I/III (red) antibodies. Nuclei were labeled using DAPI(blue). A: 10× magnification; B: 40× magnification. The results indicate that DV PI001-F are positive for Collagen I/III, afunctional characteristic of fibroblasts.

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Figure 3. Collagen Synthesis in Skin Fibroblasts. Pre-natal (PI001-F) and adult (AI001-F) skin fibroblasts and umbilical vein endothelial cells (AC005-F) were cultured in I-GRO medium to near confluence and treated with various TGF-ß-1 concentrations for 7 days. Cells were fixed, stained with Direct Red 80 (Sirius Red F3BA), solubilized, and the amount of Direct Red 80 stain, which is proportional to collagen contents, was determined by absorbance at 540nm. Parallel cultures were stained with Neutral Red, solubilized, and the amount of neutral red, which is proportional to viable cells, was determined by absorbance at 540nm. The collagen content index is the ratio of Direct Red 80 to Neutral Red. Error bars represent 95% confidence intervals.

REFERENCES1. Asada N, et al. 2011. Dysfunction of fibroblasts of extrarenal origin underlies renal fibrosis and renal anemia in mice. J Clin Invest 121(10):3981-3990.2. Otomo T, et al. 2011. Lysosomal Storage Causes Cellular Dysfunction in Mucolipidosis II Skin Fibroblasts. J Biol Chem 286(40):35283–35290.Takii T, et al. 2002.3. Simple Fibroblast-Based Assay for Screening of New Antimicrobial Drugs against Mycobacterium tuberculosis. Antimicrob Agents Chemother 46(8):2533-2539

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