identifying research resources should be easy anita bandrowski, ucsd
TRANSCRIPT
Identifying research resources should be easy
Anita Bandrowski, UCSD
A system to identify not just who produced a finding, but what produced it
Faulty Antibodies Continue to Enter US and European Markets, Warns Top Clinical Chemistry Researcher-Genome Web Daily, October 11, 2013
“…of the findings in the literature about neuronal NF-κB are based on data garnered with antibodies that are not selective for the NF-κB …”
--Herkenham et al. 2011
What studies used my monoclonal mouse antibody against actin in humans?
The following antibodies were used for immunoblotting: -actin mAb (1:10,000 dilution, Sigma-Aldrich); -tubulin mAb (1:10,000, Abcam); T46 mAb (specific to tau 404–441, 1:1000, Invitrogen); Tau-5 mAb (human tau 218–225, 1:1000, BD Biosciences) (Porzig et al., 2007); AT8 mAb (phospho-tau Ser199, Ser202, and Thr205, 1:500, Innogenetics); PHF-1 mAb (phospho-tau Ser396 and Ser404, 1:250, gift from P. Davies); 12E8 mAb (phospho-tau Ser262 and Ser356, 1:1000, gift from P. Seubert); NMDA receptors 2A, 2B and 2D goat pAbs (C terminus, 1:1000, Santa Cruz Biotechnology)…
…surely this you have found a terrible paper, this can’t be
the norm
Hypothesis: Resources in the published literature are not uniquely identifiable
Gather journal articles
5 domains:ImmunologyCell biologyNeuroscienceDevelopmental biologyGeneral biology
3 impact factors:HighMediumLow
84 Journals
238 papers
707 antibodies
104 cell lines
258 constructs
210 knockdown reagents
437 model organisms
Vasilevsky et al, PeerJ, 2013
The problem is
general across multiple resource types and disciplines
Vasilevsky et al, PeerJ, 2013
Resource Identification Initiative• Two pre-meetings with editors
and publishers– Society for Neuroscience, 2012– NIH: June, 2013– Society for Neuroscience, 2013
• Designed pilot project– Entities– Procedure– Infrastructure
• Established working group through FORCE11
• Signed up partnersLed by: Matt Brush, Nicole Vasilevsky, Anita Bandrowski and more
https://www.force11.org/Resource_identification_initiative
Pilot Project• Authors to identify 3 types of
research resources:– Software/databases– Antibodies– Model organisms
• Include RRID in methods section
• Voluntary for authors• Journals did not have to modify
their submission system• Journals have flexibility in
implementation. Send request to author at:– Submission– During review– After acceptance
Launched February 2014: 3 month commitment and more…
Resource IDs from NIF aggregated databases
•A single portal for authors
• >10 databases• One search interface• Simple directions• Prominent “Cite This” button
•Uniform format for citation across publishers
•Help desk for authors
RII Portal
http://scicrunch.com/resources
Current Progress• >150 articles have
appeared to date• 23 journals• >650 RRID’s
•3 removed by typesetting
•95% correct•14% false negative rate
• thousands of antibodies added from vendors, >200 added by individuals
• >90 software tools/databases were added to tool registry
Database available at: https://www.force11.org/node/5635
Chemicon – out of business, >8 yr
Millipore – just joined Merck, URL
still works
Millipore / Chemicon not a company
Authors cite ID properly
0.0
0.2
0.4
0.6
0.8
1.0
Post-PilotPre-Pilot
Antibodies Organisms Tools
Increased identifiability of resources after the Resource Identification Initiative Pilot
Update of Vasilevsky et al, PeerJ, 2013
What can we do with an RRID?
• A resolver service has been created
• 3rd party tools are being created to provide linkage between resources and papers– Utopia
prototype– ScienceDirect
http://scicrunch.com/resolver/RRID:nlx_144509
Utopia Tools
What have we learned?
• Authors are willing to adopt new types of citations
• Authors were fairly accurate at performing the task
• RRID’s resolved by search engines without requiring specialized citation services
• Citation drives registration• Clear role for repositories as authorities
What Can YOU Do?
– Authors: Use IDs in the methods section of next paper. scicrun.ch/resources *it is really easy!*
– Reviewers: You are ‘super beings’, use your status to improve the literature!
– Antibody Vendors: Register your tools! Make it easy for authors to cite you in a uniform format. Display the “proper citation” format proudly on the tool home page.
– Editors: easy to join the RII, download a sample letter from Force11, modify and send to authors.
– Central instructions to authors have been updated at Springer and Elsevier, we are working on Wiley. Any help appreciated with others.
Questions or Complaints: [email protected]