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Circuit Result Overview Modeling Human Practice

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Circuit ResultOverview Modeling Human

Practice

Â�õèıĴĩÂ�õèıĴĩOverview Circuit Result Modeling Human Practice

Three Circuit Types

GFP 1.Original

2.Positive Feedback Faster & Higher IntensityGFP GFP GFP

3.Negative Regulation More StableGFP GFP GFP

Â�õèıĴĩÂ�õèıĴĩOverview Circuit Result Modeling Human Practice

1.Original Circuit

Double Terminator

Constitutive Promoter

GFP

Double TerminatorReporterRBS

QS Receptor

Induced Promoter

Â�õèıĴĩÂ�õèıĴĩOverview Circuit Result Modeling Human Practice

1.Original Circuit

Double Terminator

Constitutive Promoter

GFP

Double TerminatorReporterRBS

QS Receptor

Induced Promoter

Â�õèıĴĩÂ�õèıĴĩOverview Circuit Result Modeling Human Practice

1.Original Circuit

Double Terminator

Constitutive Promoter

GFP

Double TerminatorReporterRBS

QS Receptor

Induced Promoter

Â�õèıĴĩÂ�õèıĴĩOverview Circuit Result Modeling Human Practice

1.Original Circuit

Double Terminator

Constitutive Promoter

GFP

Double TerminatorReporterRBS

QS Receptor

Induced Promoter

Â�õèıĴĩÂ�õèıĴĩOverview Circuit Result Modeling Human Practice

1.Original Circuit

Double Terminator

Constitutive Promoter

GFP

Double TerminatorReporterRBS

QS Receptor

Induced Promoter

Â�õèıĴĩÂ�õèıĴĩOverview Circuit Result Modeling Human Practice

1.Original Circuit

Double Terminator

Constitutive Promoter

GFP

Double TerminatorReporterRBS

QS Receptor

Induced Promoter

Â�õèıĴĩÂ�õèıĴĩOverview Circuit Result Modeling Human Practice

1.Original Circuit

Double Terminator

Constitutive Promoter

GFP

Double TerminatorReporterRBS

QS Receptor

Induced Promoter

Â�õèıĴĩÂ�õèıĴĩOverview Circuit Result Modeling Human Practice

1.Original Circuit

Double Terminator

Constitutive Promoter

GFP

Double Terminator

GFP

ReporterRBSQS

Receptor

Induced Promoter

Â�õèıĴĩÂ�õèıĴĩOverview Circuit Result Modeling Human Practice

ELISA Plate Reader

Â�õèıĴĩÂ�õèıĴĩOverview Circuit Result Modeling Human Practice

ELISA Plate Reader

Â�õèıĴĩÂ�õèıĴĩOverview Circuit Result Modeling Human Practice

Flow Cytometry

Start

Â�õèıĴĩÂ�õèıĴĩOverview Circuit Result Modeling Human Practice

Flow Cytometry

Start

1st Hour

Â�õèıĴĩÂ�õèıĴĩOverview Circuit Result Modeling Human Practice

Flow Cytometry

Start

1st Hour 2nd Hour

Â�õèıĴĩÂ�õèıĴĩOverview Circuit Result Modeling Human Practice

Flow Cytometry

Start

1st Hour 2nd Hour 3rd Hour

Â�õèıĴĩÂ�õèıĴĩOverview Circuit Result Modeling Human Practice

Flow Cytometry

Start

1st Hour 2nd Hour 3rd Hour 4th Hour

Â�õèıĴĩÂ�õèıĴĩOverview Circuit Result Modeling Human Practice

2. Positive Feedback Circuit

pRhIRhIRGFP

Â�õèıĴĩÂ�õèıĴĩOverview Circuit Result Modeling Human Practice

2. Positive Feedback Circuit

pRhIRhIRGFP

Â�õèıĴĩÂ�õèıĴĩOverview Circuit Result Modeling Human Practice

2. Positive Feedback Circuit

pRhIRhIRRhIR GFP

Â�õèıĴĩÂ�õèıĴĩOverview Circuit Result Modeling Human Practice

2. Positive Feedback Circuit

pRhIRhIRRhIR GFP

RhIR

Â�õèıĴĩÂ�õèıĴĩOverview Circuit Result Modeling Human Practice

2. Positive Feedback Circuit

pRhIRhIRRhIR GFP

RhIR

Â�õèıĴĩÂ�õèıĴĩOverview Circuit Result Modeling Human Practice

2. Positive Feedback Circuit

pRhIRhIRRhIR GFP

RhIR

RhIR

Â�õèıĴĩÂ�õèıĴĩOverview Circuit Result Modeling Human Practice

2. Positive Feedback Circuit

pRhIRhIRRhIR GFP

RhIR

RhIR

Â�õèıĴĩÂ�õèıĴĩOverview Circuit Result Modeling Human Practice

2. Positive Feedback Circuit

pRhIRhIRRhIR GFP

RhIR

RhIR

GFP

Â�õèıĴĩÂ�õèıĴĩOverview Circuit Result Modeling Human Practice

2. Positive Feedback Circuit

pRhIRhIRRhIR GFP

RhIR

RhIR

GFP

RhIR

Â�õèıĴĩÂ�õèıĴĩOverview Circuit Result Modeling Human Practice

2. Positive Feedback Circuit

pRhIRhIRRhIR GFP

RhIR

RhIR

GFP

RhIR

Positive Feedback

Circuit

Â�õèıĴĩÂ�õèıĴĩOverview Circuit Result Modeling Human Practice

ELISA Plate Reader

Â�õèıĴĩÂ�õèıĴĩOverview Circuit Result Modeling Human Practice

ELISA Plate Reader

Â�õèıĴĩÂ�õèıĴĩOverview Circuit Result Modeling Human Practice

Flow Cytometry

Start

Â�õèıĴĩÂ�õèıĴĩOverview Circuit Result Modeling Human Practice

Flow Cytometry

Start

1st Hour

Â�õèıĴĩÂ�õèıĴĩOverview Circuit Result Modeling Human Practice

Flow Cytometry

Start

1st Hour 2nd Hour

Â�õèıĴĩÂ�õèıĴĩOverview Circuit Result Modeling Human Practice

Flow Cytometry

Start

1st Hour 2nd Hour 3rd Hour

Â�õèıĴĩÂ�õèıĴĩOverview Circuit Result Modeling Human Practice

Flow Cytometry

Start

1st Hour 2nd Hour 3rd Hour 4th Hour

Â�õèıĴĩÂ�õèıĴĩOverview Circuit Result Modeling Human Practice

3. Negative Regulation

Divided into 3 separated steps:

Â�õèıĴĩÂ�õèıĴĩOverview Circuit Result Modeling Human Practice

3. Negative Regulation

Positive Feedback

Divided into 3 separated steps:

Â�õèıĴĩÂ�õèıĴĩOverview Circuit Result Modeling Human Practice

3. Negative Regulation

Positive Feedback Repressor

Divided into 3 separated steps:

Â�õèıĴĩÂ�õèıĴĩOverview Circuit Result Modeling Human Practice

3. Negative Regulation

Positive Feedback Repressor Reporter

Divided into 3 separated steps:

Â�õèıĴĩÂ�õèıĴĩOverview Circuit Result Modeling Human Practice

3. Negative Regulation

Positive Feedback

Repressor

Reporter

Â�õèıĴĩÂ�õèıĴĩOverview Circuit Result Modeling Human Practice

3. Negative Regulation

Positive Feedback

Repressor

Reporter

pConstLasR

Double Terminator

Â�õèıĴĩÂ�õèıĴĩOverview Circuit Result Modeling Human Practice

3. Negative Regulation

LasR

Positive Feedback

Repressor

Reporter

pConstLasR

Double Terminator

Â�õèıĴĩÂ�õèıĴĩOverview Circuit Result Modeling Human Practice

3. Negative Regulation

LasR AHL

Positive Feedback

Repressor

Reporter

pConstLasR

Double Terminator

Â�õèıĴĩÂ�õèıĴĩOverview Circuit Result Modeling Human Practice

3. Negative Regulation

LasR AHL

Positive Feedback

Repressor

Reporter

pConstLasR

Double Terminator

pLasLasR CI

Â�õèıĴĩÂ�õèıĴĩOverview Circuit Result Modeling Human Practice

3. Negative Regulation

LasR AHL

Positive Feedback

Repressor

Reporter

pConstLasR

Double Terminator

pLasLasR CI

Â�õèıĴĩÂ�õèıĴĩOverview Circuit Result Modeling Human Practice

3. Negative Regulation

LasR AHL

CI

Positive Feedback

Repressor

Reporter

pConstLasR

Double Terminator

pLasLasR CI

Â�õèıĴĩÂ�õèıĴĩOverview Circuit Result Modeling Human Practice

3. Negative Regulation

LasR AHL

CI

GFP

Positive Feedback

Repressor

ReporterpCI Luciferase/XFP

pConstLasR

Double Terminator

pLasLasR CI

Â�õèıĴĩÂ�õèıĴĩOverview Circuit Result Modeling Human Practice

3. Negative Regulation

LasR AHL

CI

GFP

Positive Feedback

Repressor

ReporterpCI Luciferase/XFP

pConstLasR

Double Terminator

pLasLasR CI

Â�õèıĴĩÂ�õèıĴĩOverview Circuit Result Modeling Human Practice

3. Negative Regulation

LasR AHL

CI

GFP

Positive Feedback

Repressor

ReporterpCI Luciferase/XFP

pConstLasR

Double Terminator

pLasLasR CI

Â�õèıĴĩÂ�õèıĴĩOverview Circuit Result Modeling Human Practice

3. Negative Regulation

LasR AHL

CI

GFP

Positive Feedback

Repressor

ReporterpCI Luciferase/XFP

pConstLasR

Double Terminator

pLasLasR CI

Â�õèıĴĩÂ�õèıĴĩOverview Circuit Result Modeling Human Practice

ELISA Plate Reader

Â�õèıĴĩÂ�õèıĴĩOverview Circuit Result Modeling Human Practice

ELISA Plate Reader

Â�õèıĴĩÂ�õèıĴĩOverview Circuit Result Modeling Human Practice

Flow Cytometry

Start

Â�õèıĴĩÂ�õèıĴĩOverview Circuit Result Modeling Human Practice

Flow Cytometry

Start

1st Hour

Â�õèıĴĩÂ�õèıĴĩOverview Circuit Result Modeling Human Practice

Flow Cytometry

Start

1st Hour 2nd Hour

Â�õèıĴĩÂ�õèıĴĩOverview Circuit Result Modeling Human Practice

Flow Cytometry

Start

1st Hour 2nd Hour 3rd Hour

Â�õèıĴĩÂ�õèıĴĩOverview Circuit Result Modeling Human Practice

Flow Cytometry

Start

1st Hour 2nd Hour 3rd Hour 4th Hour

Â�õèıĴĩÂ�õèıĴĩOverview Circuit Result Modeling Human Practice

Comparison Of Three Circuits

GFP 1.Original

2.Positive Feedback Faster & Higher IntensityGFP GFP GFP

3.Negative Regulation More StableGFP GFP GFP

Â�õèıĴĩÂ�õèıĴĩOverview Circuit Result Modeling Human Practice

Comparison Of Three Circuits

GFP 1.Original

2.Positive Feedback Faster & Higher IntensityGFP GFP GFP

3.Negative Regulation More StableGFP GFP GFP

Normal State

Â�õèıĴĩÂ�õèıĴĩOverview Circuit Result Modeling Human Practice

Comparison Of Three Circuits

GFP 1.Original

2.Positive Feedback Faster & Higher IntensityGFP GFP GFP

3.Negative Regulation More StableGFP GFP GFP

QS Signal