igem 2013 ntu-taida presentation part 2 修改後範例
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TRANSCRIPT
Â�õèıĴĩÂ�õèıĴĩOverview Circuit Result Modeling Human Practice
Three Circuit Types
GFP 1.Original
2.Positive Feedback Faster & Higher IntensityGFP GFP GFP
3.Negative Regulation More StableGFP GFP GFP
Â�õèıĴĩÂ�õèıĴĩOverview Circuit Result Modeling Human Practice
1.Original Circuit
Double Terminator
Constitutive Promoter
GFP
Double TerminatorReporterRBS
QS Receptor
Induced Promoter
Â�õèıĴĩÂ�õèıĴĩOverview Circuit Result Modeling Human Practice
1.Original Circuit
Double Terminator
Constitutive Promoter
GFP
Double TerminatorReporterRBS
QS Receptor
Induced Promoter
Â�õèıĴĩÂ�õèıĴĩOverview Circuit Result Modeling Human Practice
1.Original Circuit
Double Terminator
Constitutive Promoter
GFP
Double TerminatorReporterRBS
QS Receptor
Induced Promoter
Â�õèıĴĩÂ�õèıĴĩOverview Circuit Result Modeling Human Practice
1.Original Circuit
Double Terminator
Constitutive Promoter
GFP
Double TerminatorReporterRBS
QS Receptor
Induced Promoter
Â�õèıĴĩÂ�õèıĴĩOverview Circuit Result Modeling Human Practice
1.Original Circuit
Double Terminator
Constitutive Promoter
GFP
Double TerminatorReporterRBS
QS Receptor
Induced Promoter
Â�õèıĴĩÂ�õèıĴĩOverview Circuit Result Modeling Human Practice
1.Original Circuit
Double Terminator
Constitutive Promoter
GFP
Double TerminatorReporterRBS
QS Receptor
Induced Promoter
Â�õèıĴĩÂ�õèıĴĩOverview Circuit Result Modeling Human Practice
1.Original Circuit
Double Terminator
Constitutive Promoter
GFP
Double TerminatorReporterRBS
QS Receptor
Induced Promoter
Â�õèıĴĩÂ�õèıĴĩOverview Circuit Result Modeling Human Practice
1.Original Circuit
Double Terminator
Constitutive Promoter
GFP
Double Terminator
GFP
ReporterRBSQS
Receptor
Induced Promoter
Â�õèıĴĩÂ�õèıĴĩOverview Circuit Result Modeling Human Practice
Flow Cytometry
Start
1st Hour 2nd Hour
Â�õèıĴĩÂ�õèıĴĩOverview Circuit Result Modeling Human Practice
Flow Cytometry
Start
1st Hour 2nd Hour 3rd Hour
Â�õèıĴĩÂ�õèıĴĩOverview Circuit Result Modeling Human Practice
Flow Cytometry
Start
1st Hour 2nd Hour 3rd Hour 4th Hour
Â�õèıĴĩÂ�õèıĴĩOverview Circuit Result Modeling Human Practice
2. Positive Feedback Circuit
pRhIRhIRGFP
Â�õèıĴĩÂ�õèıĴĩOverview Circuit Result Modeling Human Practice
2. Positive Feedback Circuit
pRhIRhIRGFP
Â�õèıĴĩÂ�õèıĴĩOverview Circuit Result Modeling Human Practice
2. Positive Feedback Circuit
pRhIRhIRRhIR GFP
Â�õèıĴĩÂ�õèıĴĩOverview Circuit Result Modeling Human Practice
2. Positive Feedback Circuit
pRhIRhIRRhIR GFP
RhIR
Â�õèıĴĩÂ�õèıĴĩOverview Circuit Result Modeling Human Practice
2. Positive Feedback Circuit
pRhIRhIRRhIR GFP
RhIR
Â�õèıĴĩÂ�õèıĴĩOverview Circuit Result Modeling Human Practice
2. Positive Feedback Circuit
pRhIRhIRRhIR GFP
RhIR
RhIR
Â�õèıĴĩÂ�õèıĴĩOverview Circuit Result Modeling Human Practice
2. Positive Feedback Circuit
pRhIRhIRRhIR GFP
RhIR
RhIR
Â�õèıĴĩÂ�õèıĴĩOverview Circuit Result Modeling Human Practice
2. Positive Feedback Circuit
pRhIRhIRRhIR GFP
RhIR
RhIR
GFP
Â�õèıĴĩÂ�õèıĴĩOverview Circuit Result Modeling Human Practice
2. Positive Feedback Circuit
pRhIRhIRRhIR GFP
RhIR
RhIR
GFP
RhIR
Â�õèıĴĩÂ�õèıĴĩOverview Circuit Result Modeling Human Practice
2. Positive Feedback Circuit
pRhIRhIRRhIR GFP
RhIR
RhIR
GFP
RhIR
Positive Feedback
Circuit
Â�õèıĴĩÂ�õèıĴĩOverview Circuit Result Modeling Human Practice
Flow Cytometry
Start
1st Hour 2nd Hour
Â�õèıĴĩÂ�õèıĴĩOverview Circuit Result Modeling Human Practice
Flow Cytometry
Start
1st Hour 2nd Hour 3rd Hour
Â�õèıĴĩÂ�õèıĴĩOverview Circuit Result Modeling Human Practice
Flow Cytometry
Start
1st Hour 2nd Hour 3rd Hour 4th Hour
Â�õèıĴĩÂ�õèıĴĩOverview Circuit Result Modeling Human Practice
3. Negative Regulation
Divided into 3 separated steps:
Â�õèıĴĩÂ�õèıĴĩOverview Circuit Result Modeling Human Practice
3. Negative Regulation
Positive Feedback
Divided into 3 separated steps:
Â�õèıĴĩÂ�õèıĴĩOverview Circuit Result Modeling Human Practice
3. Negative Regulation
Positive Feedback Repressor
Divided into 3 separated steps:
Â�õèıĴĩÂ�õèıĴĩOverview Circuit Result Modeling Human Practice
3. Negative Regulation
Positive Feedback Repressor Reporter
Divided into 3 separated steps:
Â�õèıĴĩÂ�õèıĴĩOverview Circuit Result Modeling Human Practice
3. Negative Regulation
Positive Feedback
Repressor
Reporter
Â�õèıĴĩÂ�õèıĴĩOverview Circuit Result Modeling Human Practice
3. Negative Regulation
Positive Feedback
Repressor
Reporter
pConstLasR
Double Terminator
Â�õèıĴĩÂ�õèıĴĩOverview Circuit Result Modeling Human Practice
3. Negative Regulation
LasR
Positive Feedback
Repressor
Reporter
pConstLasR
Double Terminator
Â�õèıĴĩÂ�õèıĴĩOverview Circuit Result Modeling Human Practice
3. Negative Regulation
LasR AHL
Positive Feedback
Repressor
Reporter
pConstLasR
Double Terminator
Â�õèıĴĩÂ�õèıĴĩOverview Circuit Result Modeling Human Practice
3. Negative Regulation
LasR AHL
Positive Feedback
Repressor
Reporter
pConstLasR
Double Terminator
pLasLasR CI
Â�õèıĴĩÂ�õèıĴĩOverview Circuit Result Modeling Human Practice
3. Negative Regulation
LasR AHL
Positive Feedback
Repressor
Reporter
pConstLasR
Double Terminator
pLasLasR CI
Â�õèıĴĩÂ�õèıĴĩOverview Circuit Result Modeling Human Practice
3. Negative Regulation
LasR AHL
CI
Positive Feedback
Repressor
Reporter
pConstLasR
Double Terminator
pLasLasR CI
Â�õèıĴĩÂ�õèıĴĩOverview Circuit Result Modeling Human Practice
3. Negative Regulation
LasR AHL
CI
GFP
Positive Feedback
Repressor
ReporterpCI Luciferase/XFP
pConstLasR
Double Terminator
pLasLasR CI
Â�õèıĴĩÂ�õèıĴĩOverview Circuit Result Modeling Human Practice
3. Negative Regulation
LasR AHL
CI
GFP
Positive Feedback
Repressor
ReporterpCI Luciferase/XFP
pConstLasR
Double Terminator
pLasLasR CI
Â�õèıĴĩÂ�õèıĴĩOverview Circuit Result Modeling Human Practice
3. Negative Regulation
LasR AHL
CI
GFP
Positive Feedback
Repressor
ReporterpCI Luciferase/XFP
pConstLasR
Double Terminator
pLasLasR CI
Â�õèıĴĩÂ�õèıĴĩOverview Circuit Result Modeling Human Practice
3. Negative Regulation
LasR AHL
CI
GFP
Positive Feedback
Repressor
ReporterpCI Luciferase/XFP
pConstLasR
Double Terminator
pLasLasR CI
Â�õèıĴĩÂ�õèıĴĩOverview Circuit Result Modeling Human Practice
Flow Cytometry
Start
1st Hour 2nd Hour
Â�õèıĴĩÂ�õèıĴĩOverview Circuit Result Modeling Human Practice
Flow Cytometry
Start
1st Hour 2nd Hour 3rd Hour
Â�õèıĴĩÂ�õèıĴĩOverview Circuit Result Modeling Human Practice
Flow Cytometry
Start
1st Hour 2nd Hour 3rd Hour 4th Hour
Â�õèıĴĩÂ�õèıĴĩOverview Circuit Result Modeling Human Practice
Comparison Of Three Circuits
GFP 1.Original
2.Positive Feedback Faster & Higher IntensityGFP GFP GFP
3.Negative Regulation More StableGFP GFP GFP
Â�õèıĴĩÂ�õèıĴĩOverview Circuit Result Modeling Human Practice
Comparison Of Three Circuits
GFP 1.Original
2.Positive Feedback Faster & Higher IntensityGFP GFP GFP
3.Negative Regulation More StableGFP GFP GFP
Normal State