image-pro premier basic training course part 5 - automated counting
TRANSCRIPT
Image-Pro PremierBasic Training Course
Part 5 - Automated Counting
Automated Counting
Manual measurements take time and are difficult to repeat reliably
Objects in images often share intensity characteristics that are different from background or other object
By identifying groups of pixels based on pixel information we can automatically measure objects
Automated Counting
1.Segment image
2.Set up counting option
3.Select measurement types
4.Count
5.Display and save/export data
Segmentation
Divide pixels from the image into segments corresponding to:
Objects or one or more class
Background
Image-Pro can automatically identify bright or dark objects
Manual segmentation give more flexibility
Automatically Segmentation
Go to Count/Size ribbon
In Segment section select Bright or Dark
Pixels identified according to the the selection are highlighted on the image
Counting Options
Display Options - Affect look of objects
Segmentation and Count Options - Affect how objects are measured
Connectivity
4 Connect 8 Connect
Only pixels sharing a side are touching
Any neighbouring pixel can be touching
Clean Borders
Clean Borders
% Surface Area - Partial objects not important, holes probably need to be ignored - Clean borders > None
Morphology - Only whole objects to be measured, holes likely part of the objects - Clean borders > All
Counting - Need to avoid double counting partial objects, 1/2 objects on borders - Clean borders > 2 Adjacent
Measurement Types
Same as manual measurements
Select measurements relevant for object type
Use measurements for filtering
Displaying Data
When objects are counted, measurement table is automatically shown
Use Measurements Histogram to show spread
Use classification to enhance display on Image
Exporting Data
Quick Save for Publication to save image with overlays
Quick Save for Analysis to save image with measurements for review later
Export function in Measurements table to save data to Excel, a text file or for importing into Premier