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Page 1: Immunoblot Software version 2.0. - testlinecd.com software V... · 1 Immunoblot Software version 2.0. User manual ... Immunoblot Software v. 2.0. – User manual 3 System ... allows

1www.testlinecd.cz

Immunoblot Software version 2.0.User manual

Catalog number: SwIm03

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Immunoblot Software2

CONTENTSImmunoblot Software v. 2.0. – User manual 3 System requirements 3Installation 4Immunoblot Software interface 6 Tool bar and Menu 6 Scanfield 6 Analysisfield 7 Evaluationtable 7 Lowbar 7Protocol processing step by step 8 Modeselection 8 Input 8 ImmunoblotBLOT/BLOT-LINEmode–selectingatypeoftheprotocol,thetestandthelot 9 BlueDivermode–plannerandsettingkits 10 Runningtheanalysis 12 PrintingtheResultsandsavingthemasPDF 13 Savingthescannedprotocol/stripholder 14 ExporttotheLaboratoryinformationsystem 14Analysis details 15 Immunoblotmode–Selectinganalysedstrips 15 Immunoblotmode–Evaluationtable 16 Bandevaluation 16 Stripdetail 17 Startline 18 Conjugatecontrol 18 Controlline 19 BlueDivermode–Evaluationtable 19 SummaryresultsandTest(X)results 20Program settings and update 21 Programsettings 21 Lotupdate 22 Kitupdate 22 Savingandloadingdescriptions 22Common issues 23 Startlinenotfound 23 Controllinenotfound(BLOT-LINEprotocols) 23 Unabletoscan 23 Unabletodownloadupdates 23 NotloadedQRcode 23

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IMMUNOBLOT SOFTWARE – USER MANUAL

Immunoblot Software is a software tool for evaluation of BLOT, BLOT-LINE and BlueBLOT-LINE tests provided by TestLine Clinical Diagnostics,s.r.o.

SYSTEM REQUIREMENTS

Minimum system requirements:

MicrosoftWindowsXPSP3orhigher NETFrameworkversion4orhigher

Anyscannercanbeusedasaninputdevice.Thescannermustbecorrectlyinstalledandmustbedetectablebytheoperationsystem.Anyprintercanbeusedasanoutputdevice.Theprintermustbecorrectlyinstalledandmustbedetectablebytheoperationsystem.

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INSTALLATION

TheImmunoblotSoftwareisdeliveredasanimmunoblot_bundle.exedistributionpacket,whichisconfiguredtobeinstalledautomaticallywithoutauser´sinter-vention.Administratorrightsarerequiredforthe installation.Youmustacceptthetermsoflicenseagreementtoinstallthesoftwarecorrectly.

All prerequisites (suchas .NET framework version4 andprevious) are alreadyincludedintheinstallationpackage.Theinstallationprogramautomaticallyde-tectsthepresenceofprerequisitesonthecomputer,andifitdoesnotfindanyprerequisites,itautomaticallyinstallsthem.

Thenselectatypeoftheinstallation:

Typical:TheImmunoblotSoftwarewithouttheusermanualandkit definitionsisinstalledintotheProgram Filesfolder.

Custom:Selecttheinstallationpathtotheprogram.Kitdefinitionsand theusermanualcanbeinstalledasanoption.

Full:TheImmunoblotSoftware,theusermanualandkitdefinitionsare installedintotheProgram Filesfolder.

TheinstallationprogramalsocreatesaniconontheDesktopandashortcutintheStartmenu.

Attheendyouareinformedthattheinstallationwassuccessful.

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INITIALIZATION

Whenyoufirststarttheprogram,itisnecessarytoinputtheactivationcode.Youcannotoperatetheprogramwithoutit.Iftheprogramisinstalledbyaservicetech-nician,heperformsactivationsimultaneouslywiththeinstallation.Otherwisepro-ceedasfollows:

MakesureyouareloggedintoWindowsasauserwithADMINISTRATORrights –thenenteredactivationcodeisvalidforallusersofthecomputer. StartImmunoblotsoftware. ContactTestLineservice(email,phone)andgivehimtwenty-characterIDcode

ofthecomputer(A). Service technician generates a twenty-character activation code and

sends/dictatesittoyou.Putthiscodeintoactivationcodefield(B). ClickOK(C)andImmunoblotsoftwarestarts.

SCANNER CALIBRATION

Toassigncorrect intensityvalues toeach line,youmustcalibrate thescanner,preferablyimmediatelyaftertheinstallationofthesoftware.Ifascanneryouaretryingtousehasnotbeencalibrated,youwillbe informedabout it(A). Ifyou confirmitbyclickingonYes,theprogramwillexecutethescanningandevaluation, buttheresultsoftheanalysismaynotbecorrect.ScannercalibrationdoesnotapplytoBlueBLOT-LINEkitswherethecalibrationscaleisalreadypartofthesuppliedscanner.The calibration is done using a special calibration protocol supplied with the program.Thecalibrationproceduregoesasfollows:

1) Insertthecalibrationprotocol(B)intothescanner.2) OpentheScanner (C)intheSettings.3) Ifyourscannerispresentinthelistofpre-calibratedscanners(D),selectits nameandclickonApply.Otherwiseselectother,enterthecalibrationpro- tocolandclickonCalibrate scanner.4) Checkwhetherthecalibrationseriesismarkedthesameintheprogram(E) asinthecalibrationprotocol(F).Ifthemarksdiffer,contactyoursupplierto receiveanewcalibrationprotocol.5) Make sure only the scanner you want to calibrate is connected to the computerandclickontheCalibrate scannerbutton(G).6) Thecalibrationcantakeseveralminutesdependingonatypeofthescanner (H). After finishing the calibration the program informs you about the result (I). If the calibra- tion fails, the scanner you used is not fit for evaluating BLOT proto- cols.Insuchcase,contact your supplier to recom- mend/provide another scanner.

A

A

B

C

B

E

C

DF

G

IH

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IMMUNOBLOT SOFTWARE INTERFACETheImmunoblotSoftwareinterfaceconsistsofasinglewindowdividedintofivefields:

A) Tool bar and MenuB) ScanfieldC) AnalysisfieldD) EvaluationtableE) Low bar

Eachfieldintheimageisassignedadescriptionlabel:

TOOL BAR AND MENU

Tool bar and Menuareusedtomanagefollowingstepsoftheevaluationprocess:

1. Openingtheimage/Scanningtheprotocol/theclamp2. Savingthescan3. Selectingatypeoftheprotocol,thetestandthelot4. Runningtheanalysis5. PrintingtheResultsdocumentandsavingitasPDF

SCAN FIELD

Itshowsanopenedorscannedprotocol.Youcanuseitforvisualcheck-upoftheprotocolaswellasforselectingparticularstripsaftercompletionoftheanalysis.Thefieldalsoallowsyoutoadjustthezoomlevel.

A

B

C

D

E

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ANALYSIS FIELD

Itshowsdetailsofindividualstripsandacolourintensitycurveofspecificantigenicbands.ItallowsyoutohandletheStart lineofthestrip,ortomanuallyexcludeantigenicbandsfromtheevaluation(seeBand evaluation).YoucanadjustthezoomlevelinthisfieldinthesamewayasintheScan field.

EVALUATION TABLE

Itshowspreviewsofanalysedstripsandtheirdescription,theintensity(%)andtheevaluationofeachantigenicband,andalsoage-neraldiagnosticclassificationoftestedsamples.Theappearanceandfunctionoftheevaluationtablevariesineachsoftwaremode.IncaseoftheBlueDivermode,italsoincludesthemethodPlanner (F).

LOW BAR

Itshowsapathtothefilewiththeanalysedscan.Iftheimagehasnotbeensavedyet,thefollowingcautionisdisplayed:„Scan has not been saved“.

F

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PROTOCOL PROCESSING STEP BY STEPThechapterdescribesindividualstepsofprocessingthescannedprotocolwithstrips,startingtheanalysis,savingandprintingtheResultsdocument.

MODE SELECTION

Thefirststepinprocessingaprotocolisthemodeselection.Thefollowingmodesareavailable:

BLOT-LINE – used to process strips type BLOT-LINE glued to the relevant TestLine protocolsandscanned.ImmunoblotBLOT-LINEmodeallowsyoutoselectandevalua- teonemethodonaprotocol.

BLOT–usedtoprocessstripstypeWesternblotgluedtotherelevantTestLinepro- tocolsandscanned.TheImmunoblotBLOTmodeallowsyoutoselectandevaluate onemethodonaprotocol.

BlueDiver–usedtoprocessstripstypeBlueBLOT-LINEwhichdonotrequiregluing andaredirectlyinsertedintothescanneralongwithaclamp.IntheBlueDivermode, multiplekitscanbeselectedandsimultaneouslyevaluated.

ModeselectionisperformedfromtheTool bar (A).Thisoptionaffectsthebehavioroftheprogramandthereforemustbedoneattheverybeginningoftheevaluationprocess.

INPUT

Theinputfortheanalysis isan imageofascannedproto-colora clamp.Both canbe loaded fromafileordirectlyscanned.

You can open the image from a file using one of the fo-llowingoptions:

A) Select File – OpenintheMenuB) ClickontheFolder icon (open image)intheTool bar

Adialogboxallowsyoutospecifyapathtothefilewiththescannedprotocol/clamp:

A

A B

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Youcanuseoneofthefollowingoptionstodirectscan:

C) Select File – ScanintheMenuD) ClickontheScan icon (scan image)intheTool bar

Iftheprotocol/clamphasbeenscannedsuccessfully,theimageisdisplayedintheScan field (E)alongwiththetext“Scan has not been saved”intheLow bar.Whenopeningtheimagefromafile,apathtothefileisdisplayedintheLow bar (F).

IMMUNOBLOT BLOT/BLOT-LINE MODE – SELECTING A TYPE OF THE PROTOCOL, THE TEST AND THE LOT

Theprograminthe Immunoblot modeallowsyoutoprocesstwotypesofpro-tocols.First,youneedtospecifywhetheryouevaluateaprotocoltypeBLOTorBLOT-LINEwhichallowsdrop-downmenuProtocol type (A) intheToolbar.

Afterselectingaprotocoltypeyoucanselectakittype.Thiscanbedoneinthedrop-down menu Protocol name (B) in theTool bar.Youcannotselect thekittypewithoutfirstselectingtheprotocoltype.

Sinceversion1.3oftheImmunoblotsoftware,therearenowavailableQRvari-antsofprotocols(C)inadditiontoclassicBLOT-LINEprotocolsknownfromprevi-ousversions.DifferencesbetweenclassicalandQRvariantareasfollows:

C D

F

E

B

A

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1) The performance of the Conjugate control is defined for QR variantsofprotocols(seechapterDetailsoftheanalysis–Conju- gatecontrol).2) QRvariantofprotocolcontainsQRcode(D)intheupperpartof aprotocol,whichallowsautomatickitdetection.

Inthecaseofscanning/openingaprotocolwithaprintedQRcode,theprogramautomaticallychoosestheProtocol type (A) andtheKit type (B).Ifautomaticloadingforsomereason(damagedQRcode)fails,continuewiththeselectionoftheprotocoltypeandthekittypeasinpreviousversionsoftheprogram(B).

ATTENTION:Inthecaseofamanualchoice,afterloadingprotocolwithQRcode(D) selectalwaysQRvariantoftheprotocol (C)!!!

Strip lotselectiondependsonthetypeofaprocessedprotocol(BLOT,BLOT-LINE).

InthecaseoftheprotocoltypeBLOT,theTool bar displays drop-down menu Strip lot (E)withaselectionofavailablelotsinthesys-tem.Ifastriplotisnotavailableinthesystem,itcanbedownloadedfromthewebsiteofthecompanyTestLineorimportedfromCD.

AsfortheprotocoltypeBLOT-LINE,theTool bar displays a text box (F),inwhichitispossibletowritethelotnumber.LotidentificationisnotmandatoryforBLOT-LINEprotocols.

BlueDiver MODE – PLANNER AND SETTING KITS

SincetheBlueDiver modeallowstheevaluationofseveraltypesofkitsatonetime,theupperbarwiththechoiceofthetypeofkitsisnotactiveinthismode.InsteadofitthereisthePlannerintheEvaluation field.Thiscomponentallowsyoutoassigntypesofkitstodifferentgroupsofstrips.

A

D

BC

D

C

E F

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ThePlannerisdividedintoseveralfields:

Strip table (A) –eachrowcorrespondstoonepositionintheclamp.Inthetable,itispossibletoselectoneormoreactiverows toworkwith.

Kit selection (B) –displaysalistofavailablekits. Type of sample selection (C) –allowsyoutochoosewhetherthestripattheappropriatepositioncorrespondstothesampleof

apatient,positiveornegativecontrol. Test selection (D) –ifthekitallowstoperformmoretestsonastrip(differentialdiagnosis),theusercanspecifyinthispartofthe

Planner,whichtestsaretobeanalysedandwhicharenot.

Processofplanning:

TheBlueBLOT-LINEstripscontaintwo-dimensional barcode (A) (DataMatrix)whichallows theprogram to automatically assign a kit toeach strip.After scanning theclamp,thePlannerwillbeprefilled.However,theusercanchangeprefilledlayoutofstripsandmethods.Thismaybenecessary,forexample,whentheidentificationcodeonthestrip is illegible.Whenmanuallyassigningakit toastrip,proceedasfollows:

1. Inthestriptable,markastripbyclickingorbydraggingmarkofagroup (A).2. Inthelistofavailablekits,choosearequiredkit (B).3. Chooseatypeofasample (C);eventuallyusecheckboxes (D)toactivate/deactivateindividualtestswithinthechosenmethod.4. ClickonbuttonSet (E).5. Selection/settingscanbecancelledbyclickingontheDeletebutton(F).

A

A BC

D

E

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TheimagedemonstratesasituationwherekitTAwaschosenforthefirsttwostripswhilekitTBwaschosenfortheremainingstrips.Incaseakitisnotspecifiedforastrip,thestripwillbeignored.Inadditiontoautomaticassignmentofthekittypefromthecodesonstrips,theprogramalsorecognizesabatchandautomaticallyfillsitinthePlanner (G).

RUNNING THE ANALYSIS

AfterselectingallthecriteriayoucanstarttheprotocolanalysisbyclickingontheStart icon (A)inthe Tool bar.StartingtheprocessofanalysisiscommonfortheIm-munoblotmodeandfortheBlueDiver mode.

ThenyoumustfillinthetextfieldPerformed by (B) intheTool bar.Thenameyouprovidedwillalsoappearintheprintedandsaveddocuments.

ThePerformed byfieldmustbefilledin.Theprogramdemandsyoutoenteryourname (C)otherwisetheanalysiswillnotstart.

G

F

A

B

C

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Theprotocolanalysiswilltakeabout2-15secondsde-pendingonthecapacityofthecomputer.Theanalysisconsistsofseveralstepswhichcanbefollowedthrou-ghout the process. Firstly, a location in the protocolwherethestripsarefixedisset.Thelocationisshowninsharpcontoursinthedisplayedprotocol(D).Thenin-dividualstripsarelocatedandframedinyellowcolour(E).Finally,theanalysisisperformedandtheresultsaredisplayedintheAnalysis field (F) aswellasintheEva-luation table (G).

PRINTING THE RESULTS AND SAVING THEM AS PDF

Whentheanalysisisfinished,youcanviewtheresultsindetail(seeDetails of the Analysis).

Youcanchoosebetweentwotypesofevaluationdocuments.TheSummary resultsprotocolconsistsofevaluationofallantigenbandsofallprocessedstripsbutitdoesnotincludesomedetailedinformationwhichcanbefoundintheTest (X) resultsforeachparticularstrip.Bothtypesofdocumentscanbedirectlyprinted,ortheycanbesavedasPDF.

YoucansavetheResultsasPDFusingoneofthefollowingoptions:

A) Select File – Save Summary results as PDF / Save Test (X) results as PDFintheMenu B) ClickontheiconSave Results as PDFintheTool bar

Asimilarprocedureisusedfordirectprinting.

YoucanprinttheResultsbyselectingoneofthefollowingoptions:

C) Select File – Print Summary results / Print Test (X) ResultsintheMenuD) ClickonthePrintericonintheTool bar

D

GE F

A

B

C

D

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SAVING THE SCANNED PROTOCOL/CLAMP

The scanned protocol/clamp can be saved for further archiving.Youcandoitintwoways:

A) Select File/SaveimageintheMenu B) ClickontheSave image iconintheTool bar

EXPORT TO THE LABORATORY INFORMATION SYSTEM

The Immunoblot softwareallowsyou toexportresults to the laboratory information system(LIS).Aftercarryingouttheanalysisyoucancrea-teanexportXMLfilewhichcanbeimportedtotheLIS.ToexporttoaXMLformat,youmustsetanexportdirectory intheprogram(theLISwillthenadopttheanalysisresultsfromtheexportdirectory,whichmustbesetcorrectlyintheLISaswell).YoucansettheexportdirectoryintheImmunoblot software settingsusing theExport to LIS (A). First youmust enable theexport byticking theoption (B) and thenyoucanspecifytheexportdirectory(C).

Whentheexportdirectoryisset,youcancreateanexportXMLfileintheFile – Export to LIS menu (D)afterfinishingtheanalysis.

B

A

A B

C

D

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ANALYSIS DETAILS

IMMUNOBLOT MODE – SELECTING ANALYSED STRIPS

Whenanalyzingonlysomeofthestrips,youcanselectthemintheScan fieldbyclickingontheyellowborderofthestrip(A,C).Ifthestrip is:

1. highlightedbyafullyellowframe(A)–thestripismarkedandtheresultofitsanalysisisdisplayedintheEvaluation table (B).2. justoutlinedbyayellow frame (C) – the strip isnotmarkedand the resultof itsanalysis isnotdisplayed in theEvaluation table (B).

Theunmarkedstripswillnotbeevaluated.

YoucanviewadetailofanymarkedstripintheAnalysis field (E)byclickingonthecorrespondingbandinthetable (D).Intensitycurvesofindividualantigenicbandsandtheirdescriptionsandpositionsaredisplayedforaselectedstrip.AstripwithacurrentlydisplayeddetailishighlightedinyellowcolourintheEvaluation table (D)anditsframeisshowninboldintheScan field (F).YoucanalsochangethecurrentlydisplayeddetailofthestripmanuallybyusingtheUP(↑)andDOWN(↓)cursorkeys.

F

C

AE

DB

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IMMUNOBLOT MODE – EVALUATION TABLE

Theevaluationofantigenbandsisshownvianumbers/symbolsintheEvaluation tableorgraphicallyintheScan field.Appearanceofthe Evaluation tableintheImmunoblotmodeandtheBlueDivermodeisdifferent.TheBlueDivermodewillbedescribedattheendofthechapter.

Explanationoflabels,numbersandsymbolsintheEvaluation table:

A) Number–stripsarearrangedintheprotocolfromlefttoright.B) Description–ashorttextwhichcanbeusedtomarkthestrip.ThetextwillalsoappearintheResults.Descriptionsofstripscan bepreparedinadvance,theycanbesavedandloaded(seeSaving and loading decriptions).C) Strip–aviewofthestripforbetterorientationintheresults.Theviewofthestripcanbeswitchedoff(seeProgram settings).D) Analysis–canindicatepossibleproblemsinthecourseoftheanalysis.E) Test name–theinterpretationofthetest/testsresults.F) Validity–indicateswhethertheControl linehasbeenlocatedcorrectlyinBLOT-LINEprotocols.G) Band evaluation–percentagevalueofeachantigenicbandintensity. Insomecases,this informationisreplacedbyasymbol (see Band evaluation).

BAND EVALUATION

A) Negative bands are displayed in green colour (percentage valueof theband intensity).B) Border bands are displayed in orange colour (percentage value of the band intensity).C) Positive bands are displayed in red colour (percentage value of the band intensity).D) Bandthatdoesnotmatchcriteriaofevaluationismarkedwithagreen X [X].It isusuallyabandthatcannotbedistinguishedfrombackgroundofthestrip.E) Bands manually excludedfromthetest(e.g.dirtonthestrip,scanneretc.)are assignedadash [-].F) Bands considered suspicious by the program are assigned an exclamation mark [!]. The program recommends you to check whether they have been correctly evaluated. If theyhavenot been correctly evaluated, exclude them manuallyfromthetest.G) Ifthestripismechanicallydamaged(itistornortooshort),allthebandsare assigneda[ø] symbolandthisstripcannotbeevaluated.

In all cases listed above, you can view additional infor-mation concerning theevaluationbyplacing themouse cursoraboveagivensymbol.Asmallquestionmark (H) appears beside the cursor and the program displays asmalltextfieldnearthecursor.

AB

CF

GD E

AB

C

D

EG

H

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STRIP DETAIL

AfterfinishingtheanalysisyoucanviewadetailofaspecificanalysedstripintheAnalysis field.Thedetailedviewofthestripcanlooklikethis:

A) Strip image–positiononthestripcorrespondstoadisplayofindividualbandsintheintensitycurve.B) Interpretation of the test/tests results–colouredcircle/circlesindicatediagnosticclassificationofthestripaccordingtoindividual tests.Red = positive result,orange = borderline result,green = negative result.C) Band view and evaluation – green = negative or unsatisfactory band,orange = borderline band,red = positive band,black = manually excluded band.D) Start line–alinewhereananalyticareaofthestripbegins.Positionsofallantigenicbandsarerelatedtothisspecificline.E) Scale–showsapositionofeachbandinrelationtotheStart line.

Thedetailedviewofthestripalsoallowsyoutomanuallyincludeorexcludeeachbandfromthetest.Toexcludeabandfromthetestyoumustplacethemousecursorabovethegivenbandinthedetailedviewofthestrip.Thecursorchangesintoahandsymbol(F) and afterclickingonthebandadialogboxwillappearwhichallowsyoutoexcludethebandfromthetest (G).Theexcludedbandwillbedisplayedinblackcolour (H)andadash [-] willbeassignedtoitintheEvaluation table.Excludingabandfromthestripmeansanewevaluationofthewholestripwillbeperformedandtheinterpretationofthetestresultsmaychange.

The excluded band can be included in the test again using the same procedure.

BCD

E

A

F

G

H

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START LINE

The Start lineanditscorrectlocationarevitalforcorrectevaluationofthestrip.TheStart lineisdisplayedinbluecolourinthedetailedviewofthestrip.Ifthebandisnotcorrectlylocated,youcancorrectitspositionmanually.IftheStart lineisnotfound,theprograminformsyouabouttheproblem(seeStart line not found).

ManualshiftispossibleafterpositioningthecursorontheStart line.Thecursorchangesintohorizontal arrows (A)andashortinstruc-tiontomovetheband(B)appears.Youcanmovethebandtoanewpositionbyclickingonitanddraggingit.AftermovingtheStart lineanewstripevaluationisperformed.

Bymoving the Start line you accept responsibility for apotentialchangeofthetestevaluation.Thechangeisin-dicatedintheEvaluation table (C)andintheResults do-cuments.

CONJUGATE CONTROL

Theanalysisof theConjugate control is available in ver-sion1.3andabove,namelyforQRvariantsofBLOT-LINEprotocols(seechapterBasicprotocolprocessing–Proto-col, test and lot selection). If the user selects/loads thistypeofprotocol,anewfieldwillbeaddedintotheEvalua-tion table – Conjugate control (A).

Stripswith theConjugate control contain,besides the Control line,alsobandscorrespondingtorelevantconjugates(IgG,IgA,IgM).Thesebandsareindicatedintheanalysisinthesamemannerasthediagnosticbands(D),butjustastheCon-trol line,arenotrecordedintheEvaluation table.TheoutcomeoftheConjugate control isshownasvalid (B) or invalid (C).

A B

C

A

BC

E

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CONTROL LINE

TheControl line playsanimportantpart intheBLOT-LINEprotocols. Its intensity iscrucial forcorrectdeterminationoftheintensityofallanalysedantigenicbands.TheCont-rol linemustbecorrectly located.Anincorrect locationisindicated by a cross (B) intheValiditycolumnoftheEva-luation table. Correctly located Control line is assigned aticksymbol (A).

BlueDiver MODE – EVALUATION TABLES

IntheBlueDiver mode,theprogramallowsevaluationofseveralkitssimul-taneously.Sincethekitsdifferinthenumberandtypeoflines,itisnolongerpossibletosummarizealltheresultsintoonetable.Theprogramsolvesthissituationbyusingmoretabs.

AftertheanalysisseveraltabsappearnexttothePlannerintheEvaluation table.Thenumberofthetabscorrespondstothenumberofusedkits.Thetabsareidentifiedbykitabbreviations.Atableineachtabdisplaysallthestripswhichhavebeenanalysedbythekit.

EssentialsofalltablesaresameasintheImmunoblot mode(allmarks,coloursandsymbolshavethesamemeaning).IftherehasnotbeenakitassignedtosomestripsinthePlanner,theprogramignoresthosestripsandtheywillnotbefoundinanyofthetables.Theprogramwillnothighlightthosestripsina Scan field (A).

A

A

B

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SUMMARY RESULTS AND TEST (X) RESULTS

YoucansaveandprinttwotypesofResultsdocuments–summaryorindividual.TheSummary resultsprotocolincludesallanalysedstripsandtheirevaluation.TheTest (X) resultsprotocolincludesdetailedinformationofanindividualstrip.

The Summary results protocol includes:

A) HeadingwithlabidentificationB) ProtocoltypeandkittypeC) Lot,dateandtimeoftheprocess,anduseridentificationD) TableofstripimagesE) TableofbandevaluationandgeneralresultsofindividualtestsF) Analysis notes G) Footerwiththepathtothesavedprotocolscan

TheTest (X) results protocol includes:

A) HeadingwithlabidentificationB) ProtocoltypeandkittypeC) Lot,dateandtimeoftheprocess,anduseridentificationD) Detailed imageof the strip showing the intensity of each band and the intensitycurveofthebands(likeintheAnalysis field) E) TableofdetailedevaluationofeachbandandgeneralresultsofthestripF) Identificationsofdetectedantibodies(ifdefinedforagivenkit)G) Diseaseassociation(ifdefinedforagivenkit)H) Analysis notes I) FooterwiththepathtothesavedprotocolscanJ) SerialnumberofthestripK) Sample label

E

D

F

G

C

A

B

B

JK

CD

EF

GH I

A

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YoucanselectstripsforgeneratingindividualTest (X) results.UsePrint test (X) results or Save individual test results as PDFtoopenadialogbox (L)whereyoucanselectindividualstrips.

PROGRAM SETTINGS AND UPDATEFunctionsofcertainpartsoftheprogramcanbecustomizedaccordingtoyourneeds.

PROGRAM SETTINGS

TheprogramsettingscanbefoundintheMenu Edit – Settings (A).

Selectanitemtodisplayawindowwithsettings.YoucanchoosebetweenGeneral (B) and Network (J):

C) Language–youcanchangethelanguageoftheapplication.D) View strip–youcanswitchofftheviewintheEvaluation tabletomakethisarea moreclear.E) Panel size–youcanchangethesizeoftheProtocol field,theAnalysis field and theEvaluation table.F) Lab info –youcanidentifythelabwhichperformstheexamination.Thisinforma- tionwillbedisplayedintheheadingoffinalResultsdocuments.G) Automatic opening of PDF–savedPDFfileswillappearinasetbrowser.H) Export to LIS–allowsyoutoexportresultstothelaboratoryinformationsystem.I) Scanner calibration–allowsyoutocalibratethescanner.

TheNetwork settingsalsoallowyoutosetawebsitefordownloadingprogramupdates.

L

B J

EF

C

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G

HI

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Immunoblot Software22

LOT UPDATE

IfthelotoftheanalysedstripsisnotavailableinthesystemwhileprocessingBLOTprotocols,youcandownloaditfromtheweborimportitfromtheCD.Thelotcanbeimportedinthefollowingway:

A) Select Edit – Import LotintheMenu B) Select Import...intheLot drop-down menu

ThedialogboxImport lot allows you to enter a source and a number (C) ofthelottobeimported.ThesourcecanbetheCD (D) orthewebsite(E).YoucanchangethewebsiteusedforimportingthelotsintheNetwork settings.

Theprogramwillinformyouwhethertheoperationwassuccessful (F),orfailed (G):

Ifthelotwasimportedsuccessfully,itcanbeimmediatelyselectedandusedintheanalysis.

KIT UPDATE

YoucanupdatekittypebyselectingEdit – Update Blots (A)intheMenu.Adialogboxappearswhereyoucanselecttheupdatesource.

Clickeither from File (CD) (B)or fromWeb (C) anda listofavailablekitswill appear (D).After selectingakit (E) andclickingon theOKbutton (F) theprogramwillimportthekit.

Theprogramwill informyouwhethertheoperationwassuccessfulor fai-led.Ifthekitswereimportedsuccessfullytheyareimmediatelyavailablein theTool barandcanbeusedintheanalysis.

SAVING AND LOADING DESCRIPTIONS

Youcandescribeindividualstrips,andsaveandopenthedescriptions.Suchprocedurecanbehelpful,forexample,insituationswhenmorethanoneexaminationsareassignedtoapatient.Stripdescriptionsarefilledininadvanceandsavedinafile.Thenyoucanjustopenthemfromthefileforfollowinganalyses.

A) Strip descriptions,filledininadvancebyauser.B) TosavedescriptionsselectFile – SavedescriptionsintheMenu and specify a path to thefilewhere thedescriptionswill be saved.C) ToloaddescriptionsselectFile – LoaddescriptionsintheMenu and select a source file. The descriptionswill be loaded and displayedintheEvaluation table.

In theBluediver mode, the program allows to fill in descriptions (patientID)inthePlanner.Loadingandsavingofthesedescriptionsfrom/tothefileworksthesamewayasinthe Immunoblot mode.

AB

C

DE

F G

A

B C

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E F

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23www.testlinecd.cz

COMMON ISSUES

START LINE NOT FOUND (BLOT/BLOT-LINE PROTOCOL)

IftheStart lineofastripisnotfound,antigenicbandscan-notbeproperlylocatedandthetestwillnotbecorrectlyevaluated.Suchaproblemis indicatedintwoways–by atextinformationintheAnalysis field (A) and by an excla-mationmark(B) intheAnalysis columnintheEvaluation table.Thestripisautomaticallymarkedasnon-assessableandallitsbandsaredisplayedinblackcolour.Insuchcaseyoumust place theStart linemanually into the correctposition. The Start line of BLOT-LINE protocolsmust bepositionedontheredstripeneartheuppermarginofthestrip.

CONTROL LINE NOT FOUND (BLOT-LINE PROTOCOLS)

Usually theControl lineofBLOT-LINEstrips isnot foundwhentheStart lineisnotfoundorisincorrectlylocated.SuchproblemisindicatedbyacrossintheValidity column inthe Evaluation table (A).Youcanusuallysolvethepro-blembymovingtheStart line toacorrectposition.However,iftheStart lineislocatedcorrectly,youcanusuallysolvetheproblembyresettingslightlyitspositionbyapprox.0.5mm.

UNABLE TO SCAN

When scanningof theprotocol fails, theprogramwill report the failure. In such case,youmust contact a system integrator. The scanner has not been correctly installed in theoperatingsystemandtheprogramcannotdetectit.

UNABLE TO DOWNLOAD UPDATES

When theKit update or the Lot update from theweb fails repeatedly, theremay be a problem with Network settings. Check settings of the TestLine web address (http://testlinecd.cz/data/immunoblot)usedforupdateimporting(A).Ifthewebaddressisenteredcorrectlybuttheproblemsremain,contactasystemintegratortochecktheproxyserversettings(B)intheNetwork settings.

NOT LOADED QR CODE

In the case of scanning/opening a protocol/clampwith printedQR code, the programautomaticallychoosestheprotocoltypeandthekittype.Ifautomaticloadingforsomereason(damagedQRcode)fails,continuewiththeselectionoftheprotocoltypeandthekittypeasinpreviousversionsoftheprogram.ATTENTION:Inthecaseofamanualchoice,selectalwaystheQRvariantoftheprotocolafterloadingprotocolwithQRcode!!!

A

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AB

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TestLine Clinical Diagnostics s.r.o. Krizikova 68 612 00 Brno Czech Republic

Sales Department: Tel.: +420 549 121 205 (237, 238)E-mail: [email protected]

Orders:Tel./FAX: +420 541 243 390E-mail: [email protected]

www.testlinecd.com

Company is certified to the quality management system standards ISO 9001 and ISO 13485 for in vitro diagnostics.