immunofluorescence atlas hep-2 plus

Immunofluorescence Atlas HEp-2 Plus

© MEDIPAN & GA Generic Assays IFA Atlas HEp-2 Plus E_v004-2018-06-04

International Consensus on Antinuclear Antibody Pattern (ICAP), 2015

http://www.anapatterns.org/



International Consensus on Antinuclear Antibody Pattern (ICAP), 2015

2018 new: http://www.anapatterns.org/






Autoantigen: dsDNA, Histon, Nuclesome

Pattern: Interphase cells: homogenous staining of the nucleus Mitotic cells: chromatin area strongly positive

AC-1


Pattern: Interphase cells: fine speckled staining of the nucleus Mitotic cells: chromatin area negative

Autoantigen: SS-A (Ro60 and/or Ro52)

AC-4


Autoantigens: SS-B (La)

Pattern: Interphase cells: fine speckled staining of the nucleus Mitotic cells: chromatin area negative

AC-4


Autoantigens: RNP, Sm

Pattern: Interphase cells: coarse speckled staining of the nucleus Mitotic cells: chromatin area negative

AC-5


Pattern: Interphase cells: fine granular staining of the nucleus Mitotic cells: chromatin area negative

Autoantigen: Ku

AC-4


Autoantigen: Mi2

Pattern: Interphase cells: very fine dense granular staining of the nucleus Mitotic cells: chromatin area negative

AC-4


Pattern: Interphase cells: fine dense staining of the nucleus and staining of the nucleoli Mitotic cells: chromatin area positive with some dots

Autoantigen: Scl-70 (Topoisomerase I)

AC-29


Pattern: Interphase cells: nucleolar clumpy in the nucleus Mitotic cells: reticular staining of chromatin area

Autoantigen: Fibrillarin

AC-9


Autoantigens: Th/To

Pattern: Interphase cells: homogenous fluorescence of the nucleoli, weak granular staining of the nucleus possible Mitotic cells: chromatin area negative

AC-8


Pattern: Interphase cells: staining of the nucleoli and weak granular staining of the nucleus Mitotic cells: chromatin area negative

Autoantigen: PMScl

AC-8


Autoantigens: gp210 (nuclear pore)

Pattern: Interphase cells: fine granular staining of the nucleus with accentuation of the rim Mitotic cells: chromatin area negative

AC-12


Pattern: Interphase cells: fine linear staining of the nucelar membrane and homogenous staining of the nucleus Mitotic cells: chromatin area negative

Autoantigens: Lamin (A, B1, B2 and C)

AC-11


Pattern: Interphase cells: multiple dots (corresponds to number of chromosomes) in the nucleus Mitotic cells: Dots in chromatin area

Autoantigens: Centromere (CENP-A, -B, -C)

AC-3


Autoantigen: sp100

Pattern: Interphase cells: multiple nuclear dots in the nucleus Mitotic cells: chromatin area negative

AC-6


Pattern: Interphase cells: negative Mitotic cells: Centromere dots in prophase and metaphase, positive midbody in anaphase

Autoantigen: Centromere E

(AC-14)


Pattern: Interphase cells: one or two dots in cytoplasm close to nucleus membrane Mitotic cells one dot each at the poles of mitotic spindle

Autoantigen: Centrioles

AC-24


Autoantigen: mitotic chromosomes

Pattern: Interphase cells: negative Mitotic cells: exclusive positivity of mitotic chromatin from prophase to telophase

AC-28


Autoantigen: MSA 2

Pattern: Interphase cells: granular nuclear fluorescence in G2 phase Mitotic cells: staining of centromeres in prophase and metaphase, staining of midbody in anaphase

AC-27


Autoantigen: NUMA I

Pattern: Interphase cells: very fine dense staining of the nucleus Mitotic cells: chromatin area negative, staining of spindle poles and fibres

AC-26


Autoantigen: NUMA II

Pattern: Interphase cells: negative Mitotic cells: chromatin area negative, staining of the spindle

AC-25


Autoantigen: Ki-67

Pattern: Interphase cells: pleomorph staining of the nucleus Mitotic cells: chromatin area positive

(AC-13)


Autoantigen: PCNA

Pattern: Interphase cells: pleomorph granular pattern of the nucleus, S-phase cells stained Mitotic cells: chromatin area negative

AC-13


Pattern: Interphase cells: polar cytplasmic staining close to one side of the nucleus Mitotic cells: chromatin area negative

Autoantigens: Golgi

AC-22


Autoantigen: AMA, Mitochondria type 2 (M2)

Pattern: Interphase cells: large cytoplasmic dots organized in a network of filaments Mitotic cells: chromatin area negative

AC-21


Pattern: Interphase cells: diffuse staining of cytplasm, overlaying larger dots Mitotic cells: chromatin area negative

Autoantigen: Jo-1

AC-20


Autoantigen: PL7

Pattern: Interphase cells: diffuse staining of cytplasm Mitotic cells: chromatin area negative

AC-19


Autoantigen: PL12


AC-19


Autoantigen: SRP


AC-19


Autoantigens: Lysosomes

Pattern: Interphase cells: multiple small dots in the cytoplasm Mitotic cells: chromatin area negative

AC-18


Autoantigen: GW Bodies

Pattern: Interphase cells: multiple large dots in the cytoplasm Mitotic cells: chromatin area negative

AC-18


Autoantigens: ribosomal phosphoproteins and coilin (mixed pattern)

Pattern: ribP Interphase cells: very fine dense staining of the cytoplasm Mitotic cells: chromatin area negative Coilin Interphase cells: 2-5 dots in the nucleus Mitotic cells: chromatin area negative

AC-19, AC-7


Autoantigens: Vimentine

Pattern: Interphase cells: fine filamentous staining of the cytoplasm Mitotic cells: covered with multiple dots

AC-16


Autoantigens: Actin

Pattern: Interphase cells: rigid filamentous staining of the cytoplasm Mitotic cells: chromatin area negative

AC-15

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