improved separation of vitamin b complex and folic acid using some new solvent systems and...

This article was downloaded by [University of California Santa Cruz]On 02 November 2014 At 1623Publisher Taylor amp FrancisInforma Ltd Registered in England and Wales Registered Number 1072954 Registered office MortimerHouse 37-41 Mortimer Street London W1T 3JH UK

Journal of Liquid Chromatography amp RelatedTechnologiesPublication details including instructions for authors and subscription informationhttpwwwtandfonlinecomloiljlc20

IMPROVED SEPARATION OF VITAMIN B COMPLEX ANDFOLIC ACID USING SOME NEW SOLVENT SYSTEMS ANDIMPREGNATED TLCRavi Bhushan a amp Vineeta Parshad aa Chemistry Department University of Roorkee Roorkee 247 667 IndiaPublished online 16 Aug 2006

To cite this article Ravi Bhushan amp Vineeta Parshad (1999) IMPROVED SEPARATION OF VITAMIN B COMPLEX AND FOLIC ACIDUSING SOME NEW SOLVENT SYSTEMS AND IMPREGNATED TLC Journal of Liquid Chromatography amp Related Technologies2210 1607-1623 DOI 101081JLC-100101756

To link to this article httpdxdoiorg101081JLC-100101756

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J LIQ CHROM amp REL TECHNOL 22(10) 1607ndash1623 (1999)

1607

Copyright copy 1999 by Marcel Dekker Inc wwwdekkercom

IMPROVED SEPARATION OF VITAMIN BCOMPLEX AND FOLIC ACID USING SOME NEWSOLVENT SYSTEMS AND IMPREGNATED TLC

Ravi Bhushan Vineeta Parshad

Chemistry DepartmentUniversity of RoorkeeRoorkee 247 667 India

ABSTRACT

TLC separation of vitamins of the lsquoBrsquo complex group andfolic acid has been achieved on plates impregnated with differenttransition metal ions The metal ions used were Mn++ Fe++ Co++Ni++ Cu++ Zn++ and Hg++ hRf values for all the vitamins usingsix new solvent systems worked out for the purpose for each ofthe four different concentrations of each metal ion have beenreported The results have been discussed for each metal ioncompared and the best conditions of separation have beenidentified

INTRODUCTION

The water soluble group of vitamins plays a very important role in theaction of certain enzymes acting as coenzymes along with the prevention ofsome diseases Trace analysis of vitamin-B complex becomes indispensable asit is the monitor of the water soluble group1 Also the vitamins of the Bcomplex group are responsible for the healthy functioning of the musclesnerves gastro-intestinal system skin and blood Deficiency of these may leadto diseases such as beri-beri macrocyclic anaemia chellosis and perniciousanaemia There are many reports using HPLC23 GC4 HPCE5 and CZE6 for theseparation of water soluble vitamins

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1608 BHUSHAN AND PARSHAD

TLC separation of mixtures of vitamin-B complex7 acid labile cobalamin8

vitamin B69 vitamin B210 and folic acid11 have also been reported

Impregnating reagents have been reported to improve the separation of a numberof compounds12 TLC resolution of constituents of vitamin-B complex13-15 onimpregnated plates have also been described by some workers Neverthelessless work has been done on separation of vitamins using impregnated TLC

Simultaneous analysis of vitamin B1 B2 B6 B12 and folic acid is veryimportant for quality control of multivitamin preparations So studies on theuse of impregnated plates for the separation of these vitamins were made and sixnew improved solvent systems were worked out the results obtained arepresented in this paper

EXPERIMENTAL

Vitamin samples analyzed were B1 B2 B6 B12 and folic acid Thesesamples were purchased from BDH (India) Glaxo (India) and Cyanamid(India) All the impregnating reagents of AR Grade were from E Merck(Bombay) The silica gel G (E Merck (India) Ltd Bombay) with CaSO4

(13) iron chloride (003 each) and giving pH 7 in an aqueous suspension(10) was used and solvents used were also from E Merck (Bombay)

Vitamin samples were extracted from commercial tablets capsules andinjection vials available for individual components The tablets (containing 200mg of vitamin) were ground to a fine powder and extracted with absoluteethanol (20 mL) thrice The mother liquor was decanted and the crystals werewashed with a little ether The purity of each vitamin was confirmed by mpand by recording UV spectra16 The yield was about 75 The solutions ofvitamins (10-3M) were prepared in 70 ethanol

Thin layer plates (20cm X 20cm X 05 mm) were prepared by spreading aslurry of silica gel G in distilled water in a ratio of 12 with the help of Stahltype applicator The plates were then dried overnight at 50 plusmn 2 oC in an ovenFor impregnated plates the slurry was prepared in aqueous solutions of differentmetal ions the ions used were Mn++ Fe++ Co++ Ni++ Cu++ Cd++ Zn++ or Hg++

and 01 02 03 04 of each of these ions was used

Samples were applied at the 500 ng level with the help of 100microL Hamiltonsyringe The chromatograms were developed during 70 minutes for a 10 cm runin all the solvent systems The plates were air dried after development VitaminB12 remained as a natural bright red spot Spots of vitamin B2 B6 and folic acidappeared yellow However vitamin B1 was located by exposing the plates toiodine vapors

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ORDER REPRINTS

VITAMIN B COMPLEX AND FOLIC ACID 1609

RESULTS AND DISCUSSION

Systematic studies were made to find out effective solvent systems for theseparation of vitamins and the following six systems were found to be successfulfor this purpose

A1 Chloroform-n-butanol-acetic acid-ammonia (4751vv)

A2 Chloroform-n-butanol-water-acetic acid-ammonia (3505505vv)

A3 Benzene-butylacetate-n-propanol-acetic acid-ammonia (14151vv)

A4 Carbon tetrachloride-butylacetate-propionic acid-ammonia (3793vv)

A5 Carbon tetrachloride-butylacetate-methanol-ammonia (1545705vv)

A6 Carbon tetrachloride-butylacetate-propionic acid-methanol-water(231053vv)

In order to improve the separation of vitamins four differentconcentrations viz 01 02 03 and 04 of each metal ion (Mg++ Fe++Co++ Ni++ Cu++ Zn++ Cd++ and Hg++) were tried Some of the best resultsshowing the influence of metal ions on chromatographic behaviour of vitaminsare shown in Tables 1-8 The results shown in each of the tables have beendiscussed in paragraphs and compared with those on plates without anyimpregnating reagents

The hRf values were affected by the concentration of impregnating reagentin all the solvent systems Each reported hRf value is the average of at leastthree or more identical runs The spots were more compact on impregnatedlayers than on plain silica gel layer The resolution possibilities of vitaminswere calculated by dividing the distance between two spot centres by the sum ofthe two spot radii and a value of 150 or more was considered as a measure ofcomplete resolution The variation in hRf values with different transition metalions can be attributed to complex formation and variation in solubilities ofcomplexes in different solvent systems or their different adsorption coefficientsduring the development of the chromatogram The effect of each metal ion atfour different concentrations in the six solvent systems is discussed below

Impregnation with MnSO4

There was a general decrease in Rf values of vitamins on platesimpregnated with MnSO4 in all the nine solvent systems in comparison to plainplates On going from 01 to 02 impregnation hRf values increased mostly

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Tab

le 1

HR

f Val

ues

of V

itam

ins

on P

late

s Im

preg

nate

d w

ith D

iffer

ent C

once

ntra

tions

of M

nSO 4

A1

()

A2

()

A3

()

A4

()

A5

()

A6

()

Sam

ple

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

No

04

04

04

04

04

04

14

02

42

83

03

81

42

82

94

02

33

03

85

03

74

84

71

02

01

51

61

00

70

70

83

22

83

94

81

40

7

26

55

55

45

66

55

36

06

36

85

24

75

06

35

85

95

56

26

06

06

17

05

85

77

05

56

25

35

36

26

8

36

25

15

75

96

24

95

85

96

65

55

65

86

05

45

65

96

96

56

56

43

53

33

13

35

95

85

86

06

53

1

46

86

56

26

46

46

25

45

47

35

86

06

46

56

26

46

27

37

17

57

65

24

94

85

1

64

51

63

64

74

50

56

03

25

55

85

02

45

03

85

4T

32

48T

50

5

14

04

24

21

21

51

01

13

8T

33

41

43

56

36

52

45

19

41

__

__

___

___

___

__

___

S

light

tai

ling

T

T

ailin

g

Sa

mp

le N

o

1

vita

min

B 1 2

vita

min

B2

3

vita

min

B6

4

folic

aci

d

5 v

itam

in B 1

2A

1 c

hlo

rofo

rm-n

-bu

tan

ol-

ace

tic a

cid

-am

mo

nia

(4

75

1

vv)

A

2 c

hlo

rofo

rm-n

-bu

tan

ol-

wa

ter-

ace

tic a

cid

-am

mo

nia

(3

50

55

05

v

v)

A3

be

nze

ne

-B

utyl

acet

ate-

n-pr

opan

ol-a

cetic

aci

d-am

mon

ia (

14

15

1 v

v)

A

4 c

arb

on

te

tra

chlo

ride

-bu

tyla

ceta

te-p

rop

ion

ic a

cid

-am

mo

nia

(3

79

3

vv)

A

5 c

arb

on

tetr

ach

lorid

e-b

uty

lace

tate

-me

tha

no

l-a

mm

on

ia (

15

45

70

5

vv)

A

6 c

arb

on

te

tra

chlo

rid

e-b

uty

lace

tate

-pro

pio

nic

aci

d-m

eth

an

ol-

wa

ter

(23

10

53

v

v)

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by [

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ity o

f C

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t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS


Tab

le 2

HR

f Val

ues

of V

itam

ins

on P

late

s Im

preg

nate

d w

ith D

iffer

ent C

once

ntra

tions

of F

eSO 4

A1

()

A2

()

A3

()

A4

()

A5

()

A6

()

Sam

ple

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

No

04

04

04

04

04

04

14

02

73

23

53

83

02

12

34

01

52

14

15

03

53

43

21

01

51

81

71

01

02

22

63

42

14

24

31

50

8

26

53

86

66

96

54

85

65

76

82

55

05

46

35

55

35

26

24

86

26

37

07

57

86

76

55

55

45

66

36

6

36

24

56

06

56

24

15

35

46

63

15

35

76

05

85

75

66

95

35

05

53

55

56

97

06

35

15

55

95

86

8

46

84

97

07

06

44

56

16

37

33

56

06

36

56

36

16

17

35

77

87

75

27

17

17

36

94

76

16

57

67

1

56

02

34

55

15

03

62

9T3

15

41

94

25

05

14

54

03

91

21

72

02

13

8T

39

48

51

50

30

51

48

19

49

__

__

___

___

___

__

___

T

Tai

ling

S

am

ple

No

1

vi

tam

in B 1

2

vita

min

B2

3 v

itam

in B

6 4

fo

lic a

cid

5

vita

min

B 12

A1

ch

loro

form

-n-b

uta

no

l-a

cetic

aci

d-a

mm

on

ia (

47

51

v

v)

A2

chl

oro

form

-n-b

uta

no

l-w

ate

r-a

cetic

aci

d-a

mm

on

ia (

35

05

50

5

vv)

A

3 b

en

zen

e-

But

ylac

etat

e-n-

prop

anol

-ace

tic a

cid-

amm

onia

(1

41

51

vv

)

A4

ca

rbo

n t

etr

ach

lorid

e-b

uty

lace

tate

-pro

pio

nic

aci

d-a

mm

on

ia (

37

93

v

v)

A5

ca

rbo

nte

tra

chlo

ride

-bu

tyla

ceta

te-m

eth

an

ol-

am

mo

nia

(1

54

57

05

v

v)

A6

ca

rbo

n t

etr

ach

lori

de

-bu

tyla

ceta

te-p

rop

ioni

c a

cid

-met

ha

no

l-w

ate

r (2

31

05

3

vv)

Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS


Tab

le 3

HR

f Val

ues

of V

itam

ins

on P

late

s Im

preg

nate

d w

ith D

iffer

ent C

once

ntra

tions

of C

oSO 4

A1

()

A2

()

A3

()

A4

()

A5

()

A6

()

Sam

ple

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

No

04

04

04

04

04

04

14

03

82

02

43

82

42

52

64

04

62

24

35

04

23

53

31

01

22

01

91

01

81

51

82

22

54

24

21

81

6

26

56

45

55

96

56

06

16

16

87

34

95

16

35

53

93

66

25

96

26

17

07

16

87

05

75

85

05

46

06

8

36

26

15

75

86

25

65

65

86

67

35

15

56

05

95

85

06

96

36

96

03

56

16

56

65

55

35

65

05

96

0

46

86

56

56

76

46

86

96

17

37

67

26

06

56

46

46

37

36

77

17

35

26

77

27

46

54

86

16

47

56

5

56

05

43

44

55

03

54

0T4

15

4T5

64

2T4

95

14

74

04

01

23

02

3T2

43

8T3

93

43

64

34

04

64

92

63

5_

__

___

___

__

___

___

_T

T

ailin

g

Sa

mp

le N

o

1

vita

min

B 1 2

vi

tam

in B

2 3

vita

min

B6

4

folic

aci

d 5

vi

tam

in B 1

2A

1 c

hlo

rofo

rm-n

-bu

tan

ol-

ace

tic a

cid

-am

mo

nia

(4

75

1

vv)

A

2 c

hlo

rofo

rm-n

-bu

tan

ol-

wa

ter-

ace

tic a

cid

-am

mo

nia

(3

50

55

05

v

v)

A3

be

nze

ne

-B

utyl

acet

ate-

n-pr

opan

ol-a

cetic

aci

d-am

mon

ia (

14

15

1 v

v)

A

4 c

arb

on

te

tra

chlo

rid

e-b

uty

lace

tate

-pro

pio

nic

aci

d-a

mm

on

ia (

37

93

v

v)

A5

ca

rbo

n

tetr

ach

lori

de

-bu

tyla

ceta

te-m

eth

an

ol-

am

mo

nia

(1

54

57

05

v

v)

A6

ca

rbo

n t

etr

ach

lori

de

-bu

tyla

ceta

te-p

rop

ioni

c a

cid

-met

ha

no

l-w

ate

r (2

31

05

3

vv)

Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS


Tab

le 4

HR

f Val

ues

of V

itam

ins

on P

late

s Im

preg

nate

d w

ith D

iffer

ent C

once

ntra

tions

of N

iSO 4

A1

()

A2

()

A3

()

A4

()

A5

()

A6

()

Sam

ple

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

No

04

04

04

04

04

04

14

02

22

83

03

80

62

42

54

03

13

83

65

04

03

23

11

01

52

82

41

02

31

51

92

72

44

22

82

61

6

26

54

75

55

86

56

35

55

76

83

46

46

06

35

23

84

66

25

26

06

17

06

96

56

65

65

65

94

26

36

5

36

25

25

25

56

25

25

65

56

61

56

56

06

05

95

04

96

96

56

76

53

56

36

66

85

45

36

34

76

76

0

46

86

55

36

26

45

95

85

97

33

06

46

36

56

45

15

57

37

27

37

35

27

57

37

66

04

76

65

27

46

1_

__

___

___

__

___

___

_T

T

ailin

g

Sa

mp

le N

o

1

vita

min

B 1 2

vi

tam

in B

2 3

vita

min

B6

4

folic

aci

d

5 v

itam

in B 1

2A

1 c

hlo

rofo

rm-n

-bu

tan

ol-

ace

tic a

cid

-am

mo

nia

(4

75

1

vv)

A

2 c

hlo

rofo

rm-n

-bu

tan

ol-

wa

ter-

ace

tic a

cid

-am

mo

nia

(3

50

55

05

v

v)

A3

be

nze

ne

-B

utyl

acet

ate-

n-pr

opan

ol-a

cetic

aci

d-am

mon

ia (

14

15

1 v

v)

A

4 c

arb

on

te

tra

chlo

ride

-bu

tyla

ceta

te-p

rop

ion

ic a

cid

-am

mo

nia

(3

79

3

vv)

A

5 c

arb

on

T

etr

ach

lori

de

-bu

tyla

ceta

te-m

eth

an

ol-

am

mo

nia

(1

54

57

05

v

v)

A6

ca

rbo

n t

etr

ach

lorid

e-b

uty

lace

tate

-pro

pio

nic

aci

d-m

eth

an

ol-

wa

ter

(23

10

53

vv

)

Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS


Tab

le 5

HR

f Val

ues

of V

itam

ins

on P

late

s Im

preg

nate

d w

ith D

iffer

ent C

once

ntra

tions

of C

uSO 4

A1

()

A2

()

A3

()

A4

()

A5

()

A6

()

Sam

ple

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

No

04

04

04

04

04

04

14

03

13

94

13

82

2T2

03

14

03

54

04

45

05

24

23

51

02

02

92

71

02

41

82

13

92

94

13

63

01

9

26

56

46

56

66

56

36

06

16

86

06

66

76

35

95

65

36

26

46

36

47

07

57

07

36

45

95

75

56

47

1

36

26

06

97

56

26

05

95

86

66

16

87

06

06

36

15

06

97

47

57

63

57

26

66

77

15

56

05

96

76

8

46

86

85

76

16

46

46

36

47

37

16

47

16

56

95

24

67

37

88

07

65

27

97

98

26

05

86

36

27

57

7

56

04

24

04

95

03

93

83

55

4T

43

45

47

51

48

36

38

12

24

26

26

38

T3

32

52

74

63

44

64

02

52

5_

__

___

___

__

___

___

_T

T

ailin

g

Sa

mp

le N

o

1

vita

min

B 1 2

vi

tam

in B

2 3

vita

min

B6

4

folic

aci

d

5 v

itam

in B 1

2A

1 c

hlo

rofo

rm-n

-bu

tan

ol-

ace

tic a

cid

-am

mo

nia

(4

75

1

vv)

A

2 c

hlo

rofo

rm-n

-bu

tan

ol-

wa

ter-

ace

tic a

cid

-am

mo

nia

(3

50

55

05

v

v)

A3

be

nze

ne

-B

utyl

acet

ate-

n-pr

opan

ol-a

cetic

aci

d-am

mon

ia (

14

15

1 v

v)

A

4 c

arb

on

te

tra

chlo

ride

-bu

tyla

ceta

te-p

rop

ion

ic a

cid

-am

mo

nia

(3

79

3

vv)

A

5 c

arb

on

T

etr

ach

lori

de

-bu

tyla

ceta

te-m

eth

an

ol-

am

mo

nia

(1

54

57

05

v

v)

A6

ca

rbo

n t

etr

ach

lorid

e-b

uty

lace

tate

-pro

pio

nic

aci

d-m

eth

an

ol-

wa

ter

(23

10

53

vv

)

Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS


Tab

le 6

HR

f Val

ues

of V

itam

ins

on P

late

s Im

preg

nate

d w

ith D

iffer

ent C

once

ntra

tions

of Z

nSO 4

A1

()

A2

()

A3

()

A4

()

A5

()

A6

()

Sam

ple

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

No

04

04

04

04

04

04

14

03

93

84

03

81

8T2

12

54

02

24

24

15

03

03

53

21

01

82

72

61

03

41

21

43

62

33

92

62

71

0

26

56

06

16

36

56

54

95

36

84

06

76

16

34

44

85

16

25

86

16

27

07

85

56

16

25

05

74

46

36

9

36

25

76

67

26

25

55

76

46

64

36

85

86

04

85

54

76

96

47

06

53

55

66

06

76

96

35

93

15

86

4

46

86

65

35

56

46

06

06

17

36

36

56

56

55

45

55

47

36

67

17

35

26

07

17

95

25

86

05

27

05

8

56

04

73

24

35

03

23

32

95

4T

37

48

46

51

34

39

36

12

22

24

25

38

T4

22

12

24

12

74

53

42

31

8_

__

___

___

__

___

___

_T

T

ailin

g

Sa

mp

le N

o

1

vita

min

B 1 2

vi

tam

in B

2 3

vita

min

B6

4

folic

aci

d

5 v

itam

in B 1

2A

1 c

hlo

rofo

rm-n

-bu

tan

ol-

ace

tic a

cid

-am

mo

nia

(4

75

1

vv)

A

2 c

hlo

rofo

rm-n

-bu

tan

ol-

wa

ter-

ace

tic a

cid

-am

mo

nia

(3

50

55

05

v

v)

A3

be

nze

ne

-B

utyl

acet

ate-

n-pr

opan

ol-a

cetic

aci

d-am

mon

ia (

14

15

1 v

v)

A

4 c

arb

on

te

tra

chlo

ride

-bu

tyla

ceta

te-p

rop

ion

ic a

cid

-am

mo

nia

(3

79

3

vv)

A

5 c

arb

on

te

tra

chlo

rid

e-b

uty

lace

tate

-me

tha

no

l-a

mm

on

ia (

15

45

70

5

vv)

A

6 c

arb

on

te

tra

chlo

rid

e-b

uty

lace

tate

-pro

pio

nic

aci

d-m

eth

an

ol-

wa

ter

(23

10

53

v

v)

Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS


Tab

le 7

HR

f Val

ues

of V

itam

ins

on P

late

s Im

preg

nate

d w

ith D

iffer

ent C

once

ntra

tions

of C

dSO 4

A1

()

A2

()

A3

()

A4

()

A5

()

A6

()

Sam

ple

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

No

04

04

04

04

04

04

14

01

72

12

53

81

11

31

54

01

74

24

15

03

63

63

01

02

33

13

01

02

41

11

52

11

43

72

62

91

1

26

54

54

64

96

55

34

84

76

84

86

65

06

35

54

74

96

24

76

56

47

07

05

96

74

74

35

14

76

66

5

36

24

94

95

16

24

34

95

36

65

96

55

16

05

85

34

25

95

27

26

43

56

35

56

15

04

95

34

06

35

4

46

86

74

16

96

45

05

05

77

36

86

96

16

56

54

84

67

36

07

67

65

26

57

07

24

15

55

84

47

46

0

56

02

32

63

35

01

42

62

55

4T

14

46

43

51

31

35

35

12

39

24

24

38

T3

01

71

93

02

44

53

31

91

8_

__

___

___

__

___

___

_T

T

ailin

g

Sa

mp

le N

o

1

vita

min

B 1 2

vi

tam

in B

2 3

vita

min

B6

4

folic

aci

d

5 v

itam

in B 1

2A

1 c

hlo

rofo

rm-n

-bu

tan

ol-

ace

tic a

cid

-am

mo

nia

(4

75

1

vv)

A

2 c

hlo

rofo

rm-n

-bu

tan

ol-

wa

ter-

ace

tic a

cid

-am

mo

nia

(3

50

55

05

v

v)

A3

be

nze

ne

-B

utyl

acet

ate-

n-pr

opan

ol-a

cetic

aci

d-am

mon

ia (

14

15

1 v

v)

A

4 c

arb

on

te

tra

chlo

ride

-bu

tyla

ceta

te-p

rop

ion

ic a

cid

-am

mo

nia

(3

79

3

vv)

A

5 c

arb

on

te

tra

chlo

rid

e-b

uty

lace

tate

-me

tha

no

l-a

mm

on

ia (

15

45

70

5

vv)

A

6 c

arb

on

te

tra

chlo

rid

e-b

uty

lace

tate

-pro

pio

nic

aci

d-m

eth

an

ol-

wa

ter

(23

10

53

v

v)

Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS


Tab

le 8

HR

f Val

ues

of V

itam

ins

on P

late

s Im

preg

nate

d w

ith D

iffer

ent C

once

ntra

tions

of H

gSO 4

A1

()

A2

()

A3

()

A4

()

A5

()

A6

()

Sam

ple

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

No

04

04

04

04

04

04

14

01

62

12

43

81

51

71

94

03

24

03

95

03

63

43

01

01

21

51

71

01

51

01

11

91

73

73

01

91

1

26

52

54

24

66

53

14

04

46

85

25

54

96

35

24

84

86

25

04

56

17

07

15

76

04

44

15

04

35

46

1

36

23

74

75

06

23

54

95

56

65

06

95

26

05

65

54

16

96

06

56

03

54

74

95

05

05

25

13

86

14

7

46

84

54

04

56

44

15

35

27

35

66

65

96

95

95

15

27

36

46

97

15

23

66

66

74

15

05

75

07

34

9

56

02

22

43

15

02

73

6T4

05

4T4

24

9T4

3

51

39

37

34

12

19

10

20

38

T2

01

012

29

38

43

33

21

16

__

__

___

___

___

__

___

S

light

tai

ling

T

T

ailin

g

Sa

mp

le N

o

1

vita

min

B 1 2

vita

min

B2

3

vita

min

B6

4

folic

aci

d

5 v

itam

in B 1

2A

1 c

hlo

rofo

rm-n

-bu

tan

ol-

ace

tic a

cid

-am

mo

nia

(4

75

1

vv)

A

2 c

hlo

rofo

rm-n

-bu

tan

ol-

wa

ter-

ace

tic a

cid

-am

mo

nia

(3

50

55

05

v

v)

A3

be

nze

ne

-B

utyl

acet

ate-

n-pr

opan

ol-a

cetic

aci

d-am

mon

ia (

14

15

1 v

v)

A

4 c

arb

on

te

tra

chlo

ride

-bu

tyla

ceta

te-p

rop

ion

ic a

cid

-am

mo

nia

(3

79

3

vv)

A

5 c

arb

on

te

tra

chlo

rid

e-b

uty

lace

tate

-me

tha

no

l-a

mm

on

ia (

15

45

70

5

vv)

A

6 c

arb

on

te

tra

chlo

rid

e-b

uty

lace

tate

-pro

pio

nic

aci

d-m

eth

an

ol-

wa

ter

(23

10

53

v

v)

Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS


in solvent systems A1 A2 A3 and A4 while these values decreased in solventA6 No definite trend was observed with solvent system A5 Varying theconcentration of MnSO4 from 02 to 03 hRf values in general increased inall the solvent systems except in solvent system A4 where these valuesdecreased Further on increasing the concentration to 04 an increase in hRf

values was observed in solvent systems A3 A4 and A5

It is evident from Table 1 that vitamins which were not resolved on plainplates in different solvent systems are resolved after impregnation with MnSO4These are vitamin B6 and B12 in solvent system A1 (at 01) in solvent systemA6 (at 02 03 and 04) vitamin B2 B6 and B12 in solvent system A2 (at01 03 and 04) vitamin B2 and B6 in solvent system A3 (at 01 to04) vitamin B1 and B12 in solvent system A5 (at 01 to 04) and vitaminB1 B2 B6 folic acid and B12 in solvent system A4 (at 01 to 04) Bestresults were achieved at 03 of MnSO4 with all the solvent systems except A1

Impregnation with FeSO4

The hRf values were generally decreased with FeSO4 impregnation incomparison to plain plates (Table 2) in all the solvent systems except solventsystem A6 where these values generally increased An increase of concentrationof impregnating reagent from 01 to 02 resulted into a general decrease inRf values in solvent system A4 while an increase in these values was observedin solvent systems A1 A2 A3 A5 and A6 On going from 02 to 03 thesevalues generally decreased in solvent system A4 only in all the other solventsystems these values increased subsequently While from 03 to 04 hRf

values decreased in all the solvent systems except in solvent system where thesevalues increased (in contrast 03)

Table 2 shows that impregnation of FeSO4 resulted into separation offollowing vitamins in comparison to plain plates vitamin B6 and B12 in solventsystems A1 and A6 (at 01 to 04) vitamin B2 B6 and folic acid in solventsystem A2 (at 01 to 04) vitamin B2 and B6 in solvent system A3 (at 01to 04) vitamin B1 B2 B6 folic acid and B12 (at 01 to 04) in solventsystem A4 (at 01 to 04) and vitamin B1 and B12 in solvent system (at 03)The best results were obtained at 01 impregnation of FeSO4 in all the solventsystems except solvent system A5

Impregnation with CoSO4

For most of the vitamins hRf values decreased in comparison to plainplates when CoSO4 was used as impregnating reagent in comparison to plainplates (Table 3) except that these were increased with solvent system A6 On

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t 16

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2 N

ovem

ber

2014

ORDER REPRINTS


comparing the effect of different concentrations of CoSO4 it was found that at01 to 02 hRf values increased in solvent systems A2 and A5 decreased insolvent systems A1 A3 A4 and A6 On increasing the concentration to 03hRf values mostly increased in every solvent system except solvent systems A4

and A5 where a decrease in these values was observed Further increasing theconcentration to 04 hRf values increased only in solvent system A4 anddecreased mostly in all the other solvent systems

Successfully resolved vitamins which were not resolved on plain plates(Table 3) are vitamin B6 and B12 in solvent systems in A1 and A6 (at 01 to04) vitamin B2 B6 and folic acid in solvent system A2 (at 01 02 and04) vitamin B2 and B6 in solvent system A3 (at 03 and 04) vitamin B1B2 B6 folic acid B12 in solvent system A4 (at 01 03 04) and vitaminB1 and B12 in solvent system A5 (at 01 to 04) Each vitamin was resolvedat 01 in solvent systems A2 A4 A5 and A6

Impregnation with NiSO4

Decreases in hRf values were observed in general using NiSO4 asimpregnating reagent with all the solvent systems in comparison to plain plates(Table 4) except for solvent systems A5 and A6 where these values generallyincreased in most of the cases On changing the concentration from 01 to02 hRf values generally increased in solvent systems A2 A4 A5 andgenerally decreased in all the other solvent systems ie solvent systems A3 andA6 No regular trend was observed with solvent system A1 Varying theconcentration from 02 to 03 hRf values decreased with solvent systems A3A4 and A5 and increased with rest of the solvent systems Whereas from 03to 04 hRf values increased in solvent systems A3 A5 and decreased withother solvent systems It was found that using solvent system A4 there was ageneral decrease in hRf values from 01 to 04

Table 4 shows the resolved vitamins on impregnated plates with NiSO4

which were not resolved earlier on plain plates These vitamins are vitamin B6

and B12 in solvent systems A1 and A6 (at 01 to 04) vitamin B2 B6 andfolic acid in solvent system A2 (at 01 to 04) vitamin B2 and folic acid insolvent system A3 (at 01 to 04) all the vitamins in solvent system A4 (at01 02 and 03) vitamin B1 and B12 in solvent system A5 (at 01) Bestresults were achieved at 01 with all the solvent systems except A3

Impregnation with CuSO4

Impregnation with CuSO4 resulted in a general decrease of hRf values incomparison to plain plates (Table 5) in all the solvent systems except solvent

Dow

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t 16

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2014

ORDER REPRINTS


systems A5 and A6 On going from 01 to 02 hRf values in generalincreased in solvent systems A1 A3 and A5 These values decreased in all theother solvent systems From 02 to 03 hRf values generally decreased insolvent systems A2 A4 and A5 Increase in these values was found in solventsystems A3 and A6 Further from 03 to 04 hRf values in general decreasedin all the solvent systems except in solvent system A4 where these valuesincreased

Table 5 shows the vitamins which resolved with CuSO4 impregnation andhad not resolved with plain plates in the same solvent system These are vitaminB6 and B12 in solvent systems A1 and A6 (at 01 to 04) vitamin B2 B6 andfolic acid in solvent system A2 (at 03) vitamin B2 and B6 in solvent systemA3 (at 03 and 04) vitamin B2 B6 folic acid and B12 in solvent system A4(at 01 to 04) vitamin B1 and B12 in solvent system A5 (at 01 02 and04) Best results were obtained at 04 in all the solvent systems except A2

Impregnation with ZnSO4

hRf values decreased in most of the cases with ZnSO4 impregnation incomparison to plain plates (Table 6) in all the developed systems except solventsystem A6 where these values increased mostly While studying the effect ofdifferent concentrations of ZnSO4 it was found that on going from 01 to02 hRf values increased mostly in solvent systems A2 A3 A4 and A5 Thesevalues decreased mostly in solvent systems A1 and A6 Varying theconcentration from 02 to 03 a decrease in hRf values was observed mostlyin solvent systems A3 and A4 These values increased in all the other solventsystems Further increasing the concentration to 04 hRf values decreased inall the solvent systems

It is evident from Table 6 that vitamins which were not resolved on plainplates in various solvent systems are resolved after impregnation with ZnSO4These are vitamin B6 and B12 in solvent systems A1 and A6 (01 to 04)vitamin B2 B6 and folic acid in solvent system A2 (01 to 04) vitamin B2and B6 in solvent system A3 (at 01 and 03) vitamin B1 B2 B6 folic acidand B12 in solvent system A4 (at 01 03 and 04) vitamin B1 and B12 insolvent system A5 (at 01 02 and 04) Best results were obtained at 03impregnation in solvent systems A1 A2 A3 A4 and A6

Impregnation with CdSO4

There was a general decrease in hRf values with CdSO4 impregnation in allthe solvent systems in comparison to plain plates (Table 7) On going from01 to 02 hRf values in general decreased in solvent systems A4 and A6

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t 16

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2014

ORDER REPRINTS


while general increase in hRf values was observed in solvent systems A2 A3and A5 On increasing the concentration from 02 to 03 hRf valuesincreased in solvent system A2 and A6 and decreased in solvent systems A3 A4and A5 Further changing the concentration from 03 to 04 hRf valuesdecreased in all the solvent systems except A3 where an increase in hRf valueswas observed

Table 7 shows the vitamins which where not resolved earlier on plainplates These are vitamins B6 and B12 in solvent systems A1 and A6 (at 01 to02) vitamin B2 B6 and folic acid in solvent system A2 (at 01 03 and04) vitamin B2 and B6 in solvent system A3 (at 01 and 02) vitamin B1B2 B6 folic acid and B12 in solvent system A4 (at 01 03 and 04)vitamin B1 and B12 in solvent system A5 (at 01 to 04) It was interesting tofind out that 01 to 04 hRf values decreased in solvent system A4 Bestresults were achieved at 04 in all solvent systems except solvent system A3

Impregnation with HgSO4

It was observed in general that use of HgSO4 as impregnating reagentresulted in decrease in hRf values with all the solvent systems in comparison toplain plates (Table 8) except solvent A6 where hRf values increased in generalIncreasing the concentration from 01 to 02 hRf values increased in solventsystems A3 A4 and A5 On going from 02 to 03 these values decreased insolvent systems A3 and A4 A general increase was found in solvent systems A1A2 A5 and A6 Further varying the concentration to 04 a decrease in hRf

values was observed in solvent system A5 where these values were increased Adecrease in hRf values from 01 to 04 was found in solvent system A4

It is clear from Table 8 that vitamins which were not resolved on plainplates in different solvent systems are resolved after impregnation with HgSO4These are vitamins B6 and B12 in solvent systems A1 and A6 (at 01 to 04)vitamin B2 B6 and folic acid in solvent system A2 (at 01 02 and 03)vitamin B2 and B6 in solvent system A3 (at 01 02 and 03) vitamin B1B2 B6 B12 and folic acid in solvent system A4 (at 01 to 04) vitamin B1and B12 in solvent system A5 (at 01 02 and 03) Best results wereobtained at 01 impregnation with solvent systems A1 A2 A4 and A6

Better resolution with disappearance of tailing in most of the cases withgeneral decrease in hRf values was observed on impregnated plates as comparedto untreated ones in all the developed solvent systems All the concentrations ofCoSO4 in solvent system A1 were unsuccessful as there was incompletepoorresolution of vitamins under study either due to tailing or close hRf values

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2 N

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2014

ORDER REPRINTS


Sometimes diffused spots were also obtained 02 Impregnation of eachmetal ion in all the six solvent systems was proved to be relatively poor inresolving vitamins on thin silica plates

It can be assumed that at these concentrations metal ions are influencingthe chromatographic behavior by complex formation The complex formedchanged the adsorptionpartition characteristics during the development ofchromatograms and better resolution of vitamin-B complex and folic acid wasachieved It can also be assumed that weak electron donation from N O or Satoms or π electron donation from the aromatic ring of the vitamins to the metalion affected the chromatographic behavior Vitamin B12 is a porphyrinderivative and porphyrins are known to form metallic complexes with metalssuch as manganese iron copper and zinc16

On the basis of observed results it was inferred that CuSO4 at 04impregnation in all the employed solvent systems (except solvent system A2)resulted in the simultaneous resolution of constituents of vitamin-B complex andfolic acid with appreciable difference in hRf values Very sharp and compactspots were obtained with CuSO4 providing improved resolution Differentconcentrations of metal ions providing successfulimproved resolution in thedeveloped solvent systems have been shown in Table 5

Constituents of vitamin-B complex and folic acid can be separated andidentified in pharmaceutical and multivitamin preparations with less runningtime as reported earlier15 By using any of the above developed solvent systemsvitamins which were not resolved on the untreated plates were resolved withmost of the impregnating reagents

ACKNOWLEDGMENTS

The authors are thankful to the Council of Scientific and IndustrialResearch (CSIR) of New Delhi India for providing financial assistanceincluding a stipend (to Vineeta Parshad) Financial assistance from theUniversity Grants Commission of India New Delhi is also gratefullyacknowledged

REFERENCES

1 E Stahl Thin Layer Chromatography 2nd Ed Springer Verlag Berlin1969 p 293

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2 D Blanco L A Sanchez M D Gutierrez J Liq Chromatogr 17 1525-1540 (1994)

3 H M Zhao Z P Zhou P Wang Sepn 11 249-251 (1994)

4 E M Patzer D M Hilker J Chromatogr 135 489-92 (1977)

5 U Jegle J Chromatogr 6 495-501 (1993)

6 R Huopalahti J Sunell J Chromatogr 636 133-136 (1993)

7 H Thielemann Pharmazie 36 574 (1980)

8 R B Silverman D Dolphin J Sunell J Chromatogr 94 273-274 (1988)

9 J D Mahuren S P Coburn Anal Biochem 8 246-249 (1977)

10 W Funk P Derr J Sunell J Planar Chromatogr 3 149-152 (1990)

11 Igolnikova N M Akhmedkodzhaeva A N Svechinkova Pharm Zh 392-93 (1992)

12 G Grassini-Strazza V Carunchio A M Girelli J Chromatogr 466 1-35(1989)

13 R Bhushan I Ali Arch Pharm 30 1186-1187 (1987)

14 P Y Yin H N Li C T Yan Sepu 12 35-36 (1994)

15 R Bhushan V Parshad Biomed Chromatogr 8 196-198 (1994)

16 S F Dyke The Chemistry of Vitamins K W Bentley ed IntersciencePublishers London 1965 p 4

Received June 3 1998Accepted June 24 1998Manuscript 4895-TLC

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J LIQ CHROM amp REL TECHNOL 22(10) 1607ndash1623 (1999)

1607

Copyright copy 1999 by Marcel Dekker Inc wwwdekkercom

IMPROVED SEPARATION OF VITAMIN BCOMPLEX AND FOLIC ACID USING SOME NEWSOLVENT SYSTEMS AND IMPREGNATED TLC

Ravi Bhushan Vineeta Parshad

Chemistry DepartmentUniversity of RoorkeeRoorkee 247 667 India

ABSTRACT

TLC separation of vitamins of the lsquoBrsquo complex group andfolic acid has been achieved on plates impregnated with differenttransition metal ions The metal ions used were Mn++ Fe++ Co++Ni++ Cu++ Zn++ and Hg++ hRf values for all the vitamins usingsix new solvent systems worked out for the purpose for each ofthe four different concentrations of each metal ion have beenreported The results have been discussed for each metal ioncompared and the best conditions of separation have beenidentified

INTRODUCTION

The water soluble group of vitamins plays a very important role in theaction of certain enzymes acting as coenzymes along with the prevention ofsome diseases Trace analysis of vitamin-B complex becomes indispensable asit is the monitor of the water soluble group1 Also the vitamins of the Bcomplex group are responsible for the healthy functioning of the musclesnerves gastro-intestinal system skin and blood Deficiency of these may leadto diseases such as beri-beri macrocyclic anaemia chellosis and perniciousanaemia There are many reports using HPLC23 GC4 HPCE5 and CZE6 for theseparation of water soluble vitamins

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EXPERIMENTAL







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ORDER REPRINTS


Tab

le 1

HR

f Val

ues

of V

itam

ins

on P

late

s Im

preg

nate

d w

ith D

iffer

ent C

once

ntra

tions

of M

nSO 4

A1

()

A2

()

A3

()

A4

()

A5

()

A6

()

Sam

ple

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

No

04

04

04

04

04

04

14

02

42

83

03

81

42

82

94

02

33

03

85

03

74

84

71

02

01

51

61

00

70

70

83

22

83

94

81

40

7

26

55

55

45

66

55

36

06

36

85

24

75

06

35

85

95

56

26

06

06

17

05

85

77

05

56

25

35

36

26

8

36

25

15

75

96

24

95

85

96

65

55

65

86

05

45

65

96

96

56

56

43

53

33

13

35

95

85

86

06

53

1

46

86

56

26

46

46

25

45

47

35

86

06

46

56

26

46

27

37

17

57

65

24

94

85

1

64

51

63

64

74

50

56

03

25

55

85

02

45

03

85

4T

32

48T

50

5

14

04

24

21

21

51

01

13

8T

33

41

43

56

36

52

45

19

41

__

__

___

___

___

__

___

S

light

tai

ling

T

T

ailin

g

Sa

mp

le N

o

1

vita

min

B 1 2

vita

min

B2

3

vita

min

B6

4

folic

aci

d

5 v

itam

in B 1

2A

1 c

hlo

rofo

rm-n

-bu

tan

ol-

ace

tic a

cid

-am

mo

nia

(4

75

1

vv)

A

2 c

hlo

rofo

rm-n

-bu

tan

ol-

wa

ter-

ace

tic a

cid

-am

mo

nia

(3

50

55

05

v

v)

A3

be

nze

ne

-B

utyl

acet

ate-

n-pr

opan

ol-a

cetic

aci

d-am

mon

ia (

14

15

1 v

v)

A

4 c

arb

on

te

tra

chlo

ride

-bu

tyla

ceta

te-p

rop

ion

ic a

cid

-am

mo

nia

(3

79

3

vv)

A

5 c

arb

on

tetr

ach

lorid

e-b

uty

lace

tate

-me

tha

no

l-a

mm

on

ia (

15

45

70

5

vv)

A

6 c

arb

on

te

tra

chlo

rid

e-b

uty

lace

tate

-pro

pio

nic

aci

d-m

eth

an

ol-

wa

ter

(23

10

53

v

v)

Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS


Tab

le 2

HR

f Val

ues

of V

itam

ins

on P

late

s Im

preg

nate

d w

ith D

iffer

ent C

once

ntra

tions

of F

eSO 4

A1

()

A2

()

A3

()

A4

()

A5

()

A6

()

Sam

ple

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

No

04

04

04

04

04

04

14

02

73

23

53

83

02

12

34

01

52

14

15

03

53

43

21

01

51

81

71

01

02

22

63

42

14

24

31

50

8

26

53

86

66

96

54

85

65

76

82

55

05

46

35

55

35

26

24

86

26

37

07

57

86

76

55

55

45

66

36

6

36

24

56

06

56

24

15

35

46

63

15

35

76

05

85

75

66

95

35

05

53

55

56

97

06

35

15

55

95

86

8

46

84

97

07

06

44

56

16

37

33

56

06

36

56

36

16

17

35

77

87

75

27

17

17

36

94

76

16

57

67

1

56

02

34

55

15

03

62

9T3

15

41

94

25

05

14

54

03

91

21

72

02

13

8T

39

48

51

50

30

51

48

19

49

__

__

___

___

___

__

___

T

Tai

ling

S

am

ple

No

1

vi

tam

in B 1

2

vita

min

B2

3 v

itam

in B

6 4

fo

lic a

cid

5

vita

min

B 12

A1

ch

loro

form

-n-b

uta

no

l-a

cetic

aci

d-a

mm

on

ia (

47

51

v

v)

A2

chl

oro

form

-n-b

uta

no

l-w

ate

r-a

cetic

aci

d-a

mm

on

ia (

35

05

50

5

vv)

A

3 b

en

zen

e-

But

ylac

etat

e-n-

prop

anol

-ace

tic a

cid-

amm

onia

(1

41

51

vv

)

A4

ca

rbo

n t

etr

ach

lorid

e-b

uty

lace

tate

-pro

pio

nic

aci

d-a

mm

on

ia (

37

93

v

v)

A5

ca

rbo

nte

tra

chlo

ride

-bu

tyla

ceta

te-m

eth

an

ol-

am

mo

nia

(1

54

57

05

v

v)

A6

ca

rbo

n t

etr

ach

lori

de

-bu

tyla

ceta

te-p

rop

ioni

c a

cid

-met

ha

no

l-w

ate

r (2

31

05

3

vv)

Dow

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ded

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Uni

vers

ity o

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2014

ORDER REPRINTS


Tab

le 3

HR

f Val

ues

of V

itam

ins

on P

late

s Im

preg

nate

d w

ith D

iffer

ent C

once

ntra

tions

of C

oSO 4

A1

()

A2

()

A3

()

A4

()

A5

()

A6

()

Sam

ple

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

No

04

04

04

04

04

04

14

03

82

02

43

82

42

52

64

04

62

24

35

04

23

53

31

01

22

01

91

01

81

51

82

22

54

24

21

81

6

26

56

45

55

96

56

06

16

16

87

34

95

16

35

53

93

66

25

96

26

17

07

16

87

05

75

85

05

46

06

8

36

26

15

75

86

25

65

65

86

67

35

15

56

05

95

85

06

96

36

96

03

56

16

56

65

55

35

65

05

96

0

46

86

56

56

76

46

86

96

17

37

67

26

06

56

46

46

37

36

77

17

35

26

77

27

46

54

86

16

47

56

5

56

05

43

44

55

03

54

0T4

15

4T5

64

2T4

95

14

74

04

01

23

02

3T2

43

8T3

93

43

64

34

04

64

92

63

5_

__

___

___

__

___

___

_T

T

ailin

g

Sa

mp

le N

o

1

vita

min

B 1 2

vi

tam

in B

2 3

vita

min

B6

4

folic

aci

d 5

vi

tam

in B 1

2A

1 c

hlo

rofo

rm-n

-bu

tan

ol-

ace

tic a

cid

-am

mo

nia

(4

75

1

vv)

A

2 c

hlo

rofo

rm-n

-bu

tan

ol-

wa

ter-

ace

tic a

cid

-am

mo

nia

(3

50

55

05

v

v)

A3

be

nze

ne

-B

utyl

acet

ate-

n-pr

opan

ol-a

cetic

aci

d-am

mon

ia (

14

15

1 v

v)

A

4 c

arb

on

te

tra

chlo

rid

e-b

uty

lace

tate

-pro

pio

nic

aci

d-a

mm

on

ia (

37

93

v

v)

A5

ca

rbo

n

tetr

ach

lori

de

-bu

tyla

ceta

te-m

eth

an

ol-

am

mo

nia

(1

54

57

05

v

v)

A6

ca

rbo

n t

etr

ach

lori

de

-bu

tyla

ceta

te-p

rop

ioni

c a

cid

-met

ha

no

l-w

ate

r (2

31

05

3

vv)

Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS


Tab

le 4

HR

f Val

ues

of V

itam

ins

on P

late

s Im

preg

nate

d w

ith D

iffer

ent C

once

ntra

tions

of N

iSO 4

A1

()

A2

()

A3

()

A4

()

A5

()

A6

()

Sam

ple

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

No

04

04

04

04

04

04

14

02

22

83

03

80

62

42

54

03

13

83

65

04

03

23

11

01

52

82

41

02

31

51

92

72

44

22

82

61

6

26

54

75

55

86

56

35

55

76

83

46

46

06

35

23

84

66

25

26

06

17

06

96

56

65

65

65

94

26

36

5

36

25

25

25

56

25

25

65

56

61

56

56

06

05

95

04

96

96

56

76

53

56

36

66

85

45

36

34

76

76

0

46

86

55

36

26

45

95

85

97

33

06

46

36

56

45

15

57

37

27

37

35

27

57

37

66

04

76

65

27

46

1_

__

___

___

__

___

___

_T

T

ailin

g

Sa

mp

le N

o

1

vita

min

B 1 2

vi

tam

in B

2 3

vita

min

B6

4

folic

aci

d

5 v

itam

in B 1

2A

1 c

hlo

rofo

rm-n

-bu

tan

ol-

ace

tic a

cid

-am

mo

nia

(4

75

1

vv)

A

2 c

hlo

rofo

rm-n

-bu

tan

ol-

wa

ter-

ace

tic a

cid

-am

mo

nia

(3

50

55

05

v

v)

A3

be

nze

ne

-B

utyl

acet

ate-

n-pr

opan

ol-a

cetic

aci

d-am

mon

ia (

14

15

1 v

v)

A

4 c

arb

on

te

tra

chlo

ride

-bu

tyla

ceta

te-p

rop

ion

ic a

cid

-am

mo

nia

(3

79

3

vv)

A

5 c

arb

on

T

etr

ach

lori

de

-bu

tyla

ceta

te-m

eth

an

ol-

am

mo

nia

(1

54

57

05

v

v)

A6

ca

rbo

n t

etr

ach

lorid

e-b

uty

lace

tate

-pro

pio

nic

aci

d-m

eth

an

ol-

wa

ter

(23

10

53

vv

)

Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS


Tab

le 5

HR

f Val

ues

of V

itam

ins

on P

late

s Im

preg

nate

d w

ith D

iffer

ent C

once

ntra

tions

of C

uSO 4

A1

()

A2

()

A3

()

A4

()

A5

()

A6

()

Sam

ple

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

No

04

04

04

04

04

04

14

03

13

94

13

82

2T2

03

14

03

54

04

45

05

24

23

51

02

02

92

71

02

41

82

13

92

94

13

63

01

9

26

56

46

56

66

56

36

06

16

86

06

66

76

35

95

65

36

26

46

36

47

07

57

07

36

45

95

75

56

47

1

36

26

06

97

56

26

05

95

86

66

16

87

06

06

36

15

06

97

47

57

63

57

26

66

77

15

56

05

96

76

8

46

86

85

76

16

46

46

36

47

37

16

47

16

56

95

24

67

37

88

07

65

27

97

98

26

05

86

36

27

57

7

56

04

24

04

95

03

93

83

55

4T

43

45

47

51

48

36

38

12

24

26

26

38

T3

32

52

74

63

44

64

02

52

5_

__

___

___

__

___

___

_T

T

ailin

g

Sa

mp

le N

o

1

vita

min

B 1 2

vi

tam

in B

2 3

vita

min

B6

4

folic

aci

d

5 v

itam

in B 1

2A

1 c

hlo

rofo

rm-n

-bu

tan

ol-

ace

tic a

cid

-am

mo

nia

(4

75

1

vv)

A

2 c

hlo

rofo

rm-n

-bu

tan

ol-

wa

ter-

ace

tic a

cid

-am

mo

nia

(3

50

55

05

v

v)

A3

be

nze

ne

-B

utyl

acet

ate-

n-pr

opan

ol-a

cetic

aci

d-am

mon

ia (

14

15

1 v

v)

A

4 c

arb

on

te

tra

chlo

ride

-bu

tyla

ceta

te-p

rop

ion

ic a

cid

-am

mo

nia

(3

79

3

vv)

A

5 c

arb

on

T

etr

ach

lori

de

-bu

tyla

ceta

te-m

eth

an

ol-

am

mo

nia

(1

54

57

05

v

v)

A6

ca

rbo

n t

etr

ach

lorid

e-b

uty

lace

tate

-pro

pio

nic

aci

d-m

eth

an

ol-

wa

ter

(23

10

53

vv

)

Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS


Tab

le 6

HR

f Val

ues

of V

itam

ins

on P

late

s Im

preg

nate

d w

ith D

iffer

ent C

once

ntra

tions

of Z

nSO 4

A1

()

A2

()

A3

()

A4

()

A5

()

A6

()

Sam

ple

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

No

04

04

04

04

04

04

14

03

93

84

03

81

8T2

12

54

02

24

24

15

03

03

53

21

01

82

72

61

03

41

21

43

62

33

92

62

71

0

26

56

06

16

36

56

54

95

36

84

06

76

16

34

44

85

16

25

86

16

27

07

85

56

16

25

05

74

46

36

9

36

25

76

67

26

25

55

76

46

64

36

85

86

04

85

54

76

96

47

06

53

55

66

06

76

96

35

93

15

86

4

46

86

65

35

56

46

06

06

17

36

36

56

56

55

45

55

47

36

67

17

35

26

07

17

95

25

86

05

27

05

8

56

04

73

24

35

03

23

32

95

4T

37

48

46

51

34

39

36

12

22

24

25

38

T4

22

12

24

12

74

53

42

31

8_

__

___

___

__

___

___

_T

T

ailin

g

Sa

mp

le N

o

1

vita

min

B 1 2

vi

tam

in B

2 3

vita

min

B6

4

folic

aci

d

5 v

itam

in B 1

2A

1 c

hlo

rofo

rm-n

-bu

tan

ol-

ace

tic a

cid

-am

mo

nia

(4

75

1

vv)

A

2 c

hlo

rofo

rm-n

-bu

tan

ol-

wa

ter-

ace

tic a

cid

-am

mo

nia

(3

50

55

05

v

v)

A3

be

nze

ne

-B

utyl

acet

ate-

n-pr

opan

ol-a

cetic

aci

d-am

mon

ia (

14

15

1 v

v)

A

4 c

arb

on

te

tra

chlo

ride

-bu

tyla

ceta

te-p

rop

ion

ic a

cid

-am

mo

nia

(3

79

3

vv)

A

5 c

arb

on

te

tra

chlo

rid

e-b

uty

lace

tate

-me

tha

no

l-a

mm

on

ia (

15

45

70

5

vv)

A

6 c

arb

on

te

tra

chlo

rid

e-b

uty

lace

tate

-pro

pio

nic

aci

d-m

eth

an

ol-

wa

ter

(23

10

53

v

v)

Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS


Tab

le 7

HR

f Val

ues

of V

itam

ins

on P

late

s Im

preg

nate

d w

ith D

iffer

ent C

once

ntra

tions

of C

dSO 4

A1

()

A2

()

A3

()

A4

()

A5

()

A6

()

Sam

ple

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

No

04

04

04

04

04

04

14

01

72

12

53

81

11

31

54

01

74

24

15

03

63

63

01

02

33

13

01

02

41

11

52

11

43

72

62

91

1

26

54

54

64

96

55

34

84

76

84

86

65

06

35

54

74

96

24

76

56

47

07

05

96

74

74

35

14

76

66

5

36

24

94

95

16

24

34

95

36

65

96

55

16

05

85

34

25

95

27

26

43

56

35

56

15

04

95

34

06

35

4

46

86

74

16

96

45

05

05

77

36

86

96

16

56

54

84

67

36

07

67

65

26

57

07

24

15

55

84

47

46

0

56

02

32

63

35

01

42

62

55

4T

14

46

43

51

31

35

35

12

39

24

24

38

T3

01

71

93

02

44

53

31

91

8_

__

___

___

__

___

___

_T

T

ailin

g

Sa

mp

le N

o

1

vita

min

B 1 2

vi

tam

in B

2 3

vita

min

B6

4

folic

aci

d

5 v

itam

in B 1

2A

1 c

hlo

rofo

rm-n

-bu

tan

ol-

ace

tic a

cid

-am

mo

nia

(4

75

1

vv)

A

2 c

hlo

rofo

rm-n

-bu

tan

ol-

wa

ter-

ace

tic a

cid

-am

mo

nia

(3

50

55

05

v

v)

A3

be

nze

ne

-B

utyl

acet

ate-

n-pr

opan

ol-a

cetic

aci

d-am

mon

ia (

14

15

1 v

v)

A

4 c

arb

on

te

tra

chlo

ride

-bu

tyla

ceta

te-p

rop

ion

ic a

cid

-am

mo

nia

(3

79

3

vv)

A

5 c

arb

on

te

tra

chlo

rid

e-b

uty

lace

tate

-me

tha

no

l-a

mm

on

ia (

15

45

70

5

vv)

A

6 c

arb

on

te

tra

chlo

rid

e-b

uty

lace

tate

-pro

pio

nic

aci

d-m

eth

an

ol-

wa

ter

(23

10

53

v

v)

Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS


Tab

le 8

HR

f Val

ues

of V

itam

ins

on P

late

s Im

preg

nate

d w

ith D

iffer

ent C

once

ntra

tions

of H

gSO 4

A1

()

A2

()

A3

()

A4

()

A5

()

A6

()

Sam

ple

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

No

04

04

04

04

04

04

14

01

62

12

43

81

51

71

94

03

24

03

95

03

63

43

01

01

21

51

71

01

51

01

11

91

73

73

01

91

1

26

52

54

24

66

53

14

04

46

85

25

54

96

35

24

84

86

25

04

56

17

07

15

76

04

44

15

04

35

46

1

36

23

74

75

06

23

54

95

56

65

06

95

26

05

65

54

16

96

06

56

03

54

74

95

05

05

25

13

86

14

7

46

84

54

04

56

44

15

35

27

35

66

65

96

95

95

15

27

36

46

97

15

23

66

66

74

15

05

75

07

34

9

56

02

22

43

15

02

73

6T4

05

4T4

24

9T4

3

51

39

37

34

12

19

10

20

38

T2

01

012

29

38

43

33

21

16

__

__

___

___

___

__

___

S

light

tai

ling

T

T

ailin

g

Sa

mp

le N

o

1

vita

min

B 1 2

vita

min

B2

3

vita

min

B6

4

folic

aci

d

5 v

itam

in B 1

2A

1 c

hlo

rofo

rm-n

-bu

tan

ol-

ace

tic a

cid

-am

mo

nia

(4

75

1

vv)

A

2 c

hlo

rofo

rm-n

-bu

tan

ol-

wa

ter-

ace

tic a

cid

-am

mo

nia

(3

50

55

05

v

v)

A3

be

nze

ne

-B

utyl

acet

ate-

n-pr

opan

ol-a

cetic

aci

d-am

mon

ia (

14

15

1 v

v)

A

4 c

arb

on

te

tra

chlo

ride

-bu

tyla

ceta

te-p

rop

ion

ic a

cid

-am

mo

nia

(3

79

3

vv)

A

5 c

arb

on

te

tra

chlo

rid

e-b

uty

lace

tate

-me

tha

no

l-a

mm

on

ia (

15

45

70

5

vv)

A

6 c

arb

on

te

tra

chlo

rid

e-b

uty

lace

tate

-pro

pio

nic

aci

d-m

eth

an

ol-

wa

ter

(23

10

53

v

v)

Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS











Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS












Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS









Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS









Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS






ACKNOWLEDGMENTS


REFERENCES


Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS


















Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

Order now








Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS






EXPERIMENTAL







Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS














Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS


Tab

le 1

HR

f Val

ues

of V

itam

ins

on P

late

s Im

preg

nate

d w

ith D

iffer

ent C

once

ntra

tions

of M

nSO 4

A1

()

A2

()

A3

()

A4

()

A5

()

A6

()

Sam

ple

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

No

04

04

04

04

04

04

14

02

42

83

03

81

42

82

94

02

33

03

85

03

74

84

71

02

01

51

61

00

70

70

83

22

83

94

81

40

7

26

55

55

45

66

55

36

06

36

85

24

75

06

35

85

95

56

26

06

06

17

05

85

77

05

56

25

35

36

26

8

36

25

15

75

96

24

95

85

96

65

55

65

86

05

45

65

96

96

56

56

43

53

33

13

35

95

85

86

06

53

1

46

86

56

26

46

46

25

45

47

35

86

06

46

56

26

46

27

37

17

57

65

24

94

85

1

64

51

63

64

74

50

56

03

25

55

85

02

45

03

85

4T

32

48T

50

5

14

04

24

21

21

51

01

13

8T

33

41

43

56

36

52

45

19

41

__

__

___

___

___

__

___

S

light

tai

ling

T

T

ailin

g

Sa

mp

le N

o

1

vita

min

B 1 2

vita

min

B2

3

vita

min

B6

4

folic

aci

d

5 v

itam

in B 1

2A

1 c

hlo

rofo

rm-n

-bu

tan

ol-

ace

tic a

cid

-am

mo

nia

(4

75

1

vv)

A

2 c

hlo

rofo

rm-n

-bu

tan

ol-

wa

ter-

ace

tic a

cid

-am

mo

nia

(3

50

55

05

v

v)

A3

be

nze

ne

-B

utyl

acet

ate-

n-pr

opan

ol-a

cetic

aci

d-am

mon

ia (

14

15

1 v

v)

A

4 c

arb

on

te

tra

chlo

ride

-bu

tyla

ceta

te-p

rop

ion

ic a

cid

-am

mo

nia

(3

79

3

vv)

A

5 c

arb

on

tetr

ach

lorid

e-b

uty

lace

tate

-me

tha

no

l-a

mm

on

ia (

15

45

70

5

vv)

A

6 c

arb

on

te

tra

chlo

rid

e-b

uty

lace

tate

-pro

pio

nic

aci

d-m

eth

an

ol-

wa

ter

(23

10

53

v

v)

Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS


Tab

le 2

HR

f Val

ues

of V

itam

ins

on P

late

s Im

preg

nate

d w

ith D

iffer

ent C

once

ntra

tions

of F

eSO 4

A1

()

A2

()

A3

()

A4

()

A5

()

A6

()

Sam

ple

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

No

04

04

04

04

04

04

14

02

73

23

53

83

02

12

34

01

52

14

15

03

53

43

21

01

51

81

71

01

02

22

63

42

14

24

31

50

8

26

53

86

66

96

54

85

65

76

82

55

05

46

35

55

35

26

24

86

26

37

07

57

86

76

55

55

45

66

36

6

36

24

56

06

56

24

15

35

46

63

15

35

76

05

85

75

66

95

35

05

53

55

56

97

06

35

15

55

95

86

8

46

84

97

07

06

44

56

16

37

33

56

06

36

56

36

16

17

35

77

87

75

27

17

17

36

94

76

16

57

67

1

56

02

34

55

15

03

62

9T3

15

41

94

25

05

14

54

03

91

21

72

02

13

8T

39

48

51

50

30

51

48

19

49

__

__

___

___

___

__

___

T

Tai

ling

S

am

ple

No

1

vi

tam

in B 1

2

vita

min

B2

3 v

itam

in B

6 4

fo

lic a

cid

5

vita

min

B 12

A1

ch

loro

form

-n-b

uta

no

l-a

cetic

aci

d-a

mm

on

ia (

47

51

v

v)

A2

chl

oro

form

-n-b

uta

no

l-w

ate

r-a

cetic

aci

d-a

mm

on

ia (

35

05

50

5

vv)

A

3 b

en

zen

e-

But

ylac

etat

e-n-

prop

anol

-ace

tic a

cid-

amm

onia

(1

41

51

vv

)

A4

ca

rbo

n t

etr

ach

lorid

e-b

uty

lace

tate

-pro

pio

nic

aci

d-a

mm

on

ia (

37

93

v

v)

A5

ca

rbo

nte

tra

chlo

ride

-bu

tyla

ceta

te-m

eth

an

ol-

am

mo

nia

(1

54

57

05

v

v)

A6

ca

rbo

n t

etr

ach

lori

de

-bu

tyla

ceta

te-p

rop

ioni

c a

cid

-met

ha

no

l-w

ate

r (2

31

05

3

vv)

Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS


Tab

le 3

HR

f Val

ues

of V

itam

ins

on P

late

s Im

preg

nate

d w

ith D

iffer

ent C

once

ntra

tions

of C

oSO 4

A1

()

A2

()

A3

()

A4

()

A5

()

A6

()

Sam

ple

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

No

04

04

04

04

04

04

14

03

82

02

43

82

42

52

64

04

62

24

35

04

23

53

31

01

22

01

91

01

81

51

82

22

54

24

21

81

6

26

56

45

55

96

56

06

16

16

87

34

95

16

35

53

93

66

25

96

26

17

07

16

87

05

75

85

05

46

06

8

36

26

15

75

86

25

65

65

86

67

35

15

56

05

95

85

06

96

36

96

03

56

16

56

65

55

35

65

05

96

0

46

86

56

56

76

46

86

96

17

37

67

26

06

56

46

46

37

36

77

17

35

26

77

27

46

54

86

16

47

56

5

56

05

43

44

55

03

54

0T4

15

4T5

64

2T4

95

14

74

04

01

23

02

3T2

43

8T3

93

43

64

34

04

64

92

63

5_

__

___

___

__

___

___

_T

T

ailin

g

Sa

mp

le N

o

1

vita

min

B 1 2

vi

tam

in B

2 3

vita

min

B6

4

folic

aci

d 5

vi

tam

in B 1

2A

1 c

hlo

rofo

rm-n

-bu

tan

ol-

ace

tic a

cid

-am

mo

nia

(4

75

1

vv)

A

2 c

hlo

rofo

rm-n

-bu

tan

ol-

wa

ter-

ace

tic a

cid

-am

mo

nia

(3

50

55

05

v

v)

A3

be

nze

ne

-B

utyl

acet

ate-

n-pr

opan

ol-a

cetic

aci

d-am

mon

ia (

14

15

1 v

v)

A

4 c

arb

on

te

tra

chlo

rid

e-b

uty

lace

tate

-pro

pio

nic

aci

d-a

mm

on

ia (

37

93

v

v)

A5

ca

rbo

n

tetr

ach

lori

de

-bu

tyla

ceta

te-m

eth

an

ol-

am

mo

nia

(1

54

57

05

v

v)

A6

ca

rbo

n t

etr

ach

lori

de

-bu

tyla

ceta

te-p

rop

ioni

c a

cid

-met

ha

no

l-w

ate

r (2

31

05

3

vv)

Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS


Tab

le 4

HR

f Val

ues

of V

itam

ins

on P

late

s Im

preg

nate

d w

ith D

iffer

ent C

once

ntra

tions

of N

iSO 4

A1

()

A2

()

A3

()

A4

()

A5

()

A6

()

Sam

ple

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

No

04

04

04

04

04

04

14

02

22

83

03

80

62

42

54

03

13

83

65

04

03

23

11

01

52

82

41

02

31

51

92

72

44

22

82

61

6

26

54

75

55

86

56

35

55

76

83

46

46

06

35

23

84

66

25

26

06

17

06

96

56

65

65

65

94

26

36

5

36

25

25

25

56

25

25

65

56

61

56

56

06

05

95

04

96

96

56

76

53

56

36

66

85

45

36

34

76

76

0

46

86

55

36

26

45

95

85

97

33

06

46

36

56

45

15

57

37

27

37

35

27

57

37

66

04

76

65

27

46

1_

__

___

___

__

___

___

_T

T

ailin

g

Sa

mp

le N

o

1

vita

min

B 1 2

vi

tam

in B

2 3

vita

min

B6

4

folic

aci

d

5 v

itam

in B 1

2A

1 c

hlo

rofo

rm-n

-bu

tan

ol-

ace

tic a

cid

-am

mo

nia

(4

75

1

vv)

A

2 c

hlo

rofo

rm-n

-bu

tan

ol-

wa

ter-

ace

tic a

cid

-am

mo

nia

(3

50

55

05

v

v)

A3

be

nze

ne

-B

utyl

acet

ate-

n-pr

opan

ol-a

cetic

aci

d-am

mon

ia (

14

15

1 v

v)

A

4 c

arb

on

te

tra

chlo

ride

-bu

tyla

ceta

te-p

rop

ion

ic a

cid

-am

mo

nia

(3

79

3

vv)

A

5 c

arb

on

T

etr

ach

lori

de

-bu

tyla

ceta

te-m

eth

an

ol-

am

mo

nia

(1

54

57

05

v

v)

A6

ca

rbo

n t

etr

ach

lorid

e-b

uty

lace

tate

-pro

pio

nic

aci

d-m

eth

an

ol-

wa

ter

(23

10

53

vv

)

Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS


Tab

le 5

HR

f Val

ues

of V

itam

ins

on P

late

s Im

preg

nate

d w

ith D

iffer

ent C

once

ntra

tions

of C

uSO 4

A1

()

A2

()

A3

()

A4

()

A5

()

A6

()

Sam

ple

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

No

04

04

04

04

04

04

14

03

13

94

13

82

2T2

03

14

03

54

04

45

05

24

23

51

02

02

92

71

02

41

82

13

92

94

13

63

01

9

26

56

46

56

66

56

36

06

16

86

06

66

76

35

95

65

36

26

46

36

47

07

57

07

36

45

95

75

56

47

1

36

26

06

97

56

26

05

95

86

66

16

87

06

06

36

15

06

97

47

57

63

57

26

66

77

15

56

05

96

76

8

46

86

85

76

16

46

46

36

47

37

16

47

16

56

95

24

67

37

88

07

65

27

97

98

26

05

86

36

27

57

7

56

04

24

04

95

03

93

83

55

4T

43

45

47

51

48

36

38

12

24

26

26

38

T3

32

52

74

63

44

64

02

52

5_

__

___

___

__

___

___

_T

T

ailin

g

Sa

mp

le N

o

1

vita

min

B 1 2

vi

tam

in B

2 3

vita

min

B6

4

folic

aci

d

5 v

itam

in B 1

2A

1 c

hlo

rofo

rm-n

-bu

tan

ol-

ace

tic a

cid

-am

mo

nia

(4

75

1

vv)

A

2 c

hlo

rofo

rm-n

-bu

tan

ol-

wa

ter-

ace

tic a

cid

-am

mo

nia

(3

50

55

05

v

v)

A3

be

nze

ne

-B

utyl

acet

ate-

n-pr

opan

ol-a

cetic

aci

d-am

mon

ia (

14

15

1 v

v)

A

4 c

arb

on

te

tra

chlo

ride

-bu

tyla

ceta

te-p

rop

ion

ic a

cid

-am

mo

nia

(3

79

3

vv)

A

5 c

arb

on

T

etr

ach

lori

de

-bu

tyla

ceta

te-m

eth

an

ol-

am

mo

nia

(1

54

57

05

v

v)

A6

ca

rbo

n t

etr

ach

lorid

e-b

uty

lace

tate

-pro

pio

nic

aci

d-m

eth

an

ol-

wa

ter

(23

10

53

vv

)

Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS


Tab

le 6

HR

f Val

ues

of V

itam

ins

on P

late

s Im

preg

nate

d w

ith D

iffer

ent C

once

ntra

tions

of Z

nSO 4

A1

()

A2

()

A3

()

A4

()

A5

()

A6

()

Sam

ple

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

No

04

04

04

04

04

04

14

03

93

84

03

81

8T2

12

54

02

24

24

15

03

03

53

21

01

82

72

61

03

41

21

43

62

33

92

62

71

0

26

56

06

16

36

56

54

95

36

84

06

76

16

34

44

85

16

25

86

16

27

07

85

56

16

25

05

74

46

36

9

36

25

76

67

26

25

55

76

46

64

36

85

86

04

85

54

76

96

47

06

53

55

66

06

76

96

35

93

15

86

4

46

86

65

35

56

46

06

06

17

36

36

56

56

55

45

55

47

36

67

17

35

26

07

17

95

25

86

05

27

05

8

56

04

73

24

35

03

23

32

95

4T

37

48

46

51

34

39

36

12

22

24

25

38

T4

22

12

24

12

74

53

42

31

8_

__

___

___

__

___

___

_T

T

ailin

g

Sa

mp

le N

o

1

vita

min

B 1 2

vi

tam

in B

2 3

vita

min

B6

4

folic

aci

d

5 v

itam

in B 1

2A

1 c

hlo

rofo

rm-n

-bu

tan

ol-

ace

tic a

cid

-am

mo

nia

(4

75

1

vv)

A

2 c

hlo

rofo

rm-n

-bu

tan

ol-

wa

ter-

ace

tic a

cid

-am

mo

nia

(3

50

55

05

v

v)

A3

be

nze

ne

-B

utyl

acet

ate-

n-pr

opan

ol-a

cetic

aci

d-am

mon

ia (

14

15

1 v

v)

A

4 c

arb

on

te

tra

chlo

ride

-bu

tyla

ceta

te-p

rop

ion

ic a

cid

-am

mo

nia

(3

79

3

vv)

A

5 c

arb

on

te

tra

chlo

rid

e-b

uty

lace

tate

-me

tha

no

l-a

mm

on

ia (

15

45

70

5

vv)

A

6 c

arb

on

te

tra

chlo

rid

e-b

uty

lace

tate

-pro

pio

nic

aci

d-m

eth

an

ol-

wa

ter

(23

10

53

v

v)

Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS


Tab

le 7

HR

f Val

ues

of V

itam

ins

on P

late

s Im

preg

nate

d w

ith D

iffer

ent C

once

ntra

tions

of C

dSO 4

A1

()

A2

()

A3

()

A4

()

A5

()

A6

()

Sam

ple

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

No

04

04

04

04

04

04

14

01

72

12

53

81

11

31

54

01

74

24

15

03

63

63

01

02

33

13

01

02

41

11

52

11

43

72

62

91

1

26

54

54

64

96

55

34

84

76

84

86

65

06

35

54

74

96

24

76

56

47

07

05

96

74

74

35

14

76

66

5

36

24

94

95

16

24

34

95

36

65

96

55

16

05

85

34

25

95

27

26

43

56

35

56

15

04

95

34

06

35

4

46

86

74

16

96

45

05

05

77

36

86

96

16

56

54

84

67

36

07

67

65

26

57

07

24

15

55

84

47

46

0

56

02

32

63

35

01

42

62

55

4T

14

46

43

51

31

35

35

12

39

24

24

38

T3

01

71

93

02

44

53

31

91

8_

__

___

___

__

___

___

_T

T

ailin

g

Sa

mp

le N

o

1

vita

min

B 1 2

vi

tam

in B

2 3

vita

min

B6

4

folic

aci

d

5 v

itam

in B 1

2A

1 c

hlo

rofo

rm-n

-bu

tan

ol-

ace

tic a

cid

-am

mo

nia

(4

75

1

vv)

A

2 c

hlo

rofo

rm-n

-bu

tan

ol-

wa

ter-

ace

tic a

cid

-am

mo

nia

(3

50

55

05

v

v)

A3

be

nze

ne

-B

utyl

acet

ate-

n-pr

opan

ol-a

cetic

aci

d-am

mon

ia (

14

15

1 v

v)

A

4 c

arb

on

te

tra

chlo

ride

-bu

tyla

ceta

te-p

rop

ion

ic a

cid

-am

mo

nia

(3

79

3

vv)

A

5 c

arb

on

te

tra

chlo

rid

e-b

uty

lace

tate

-me

tha

no

l-a

mm

on

ia (

15

45

70

5

vv)

A

6 c

arb

on

te

tra

chlo

rid

e-b

uty

lace

tate

-pro

pio

nic

aci

d-m

eth

an

ol-

wa

ter

(23

10

53

v

v)

Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS


Tab

le 8

HR

f Val

ues

of V

itam

ins

on P

late

s Im

preg

nate

d w

ith D

iffer

ent C

once

ntra

tions

of H

gSO 4

A1

()

A2

()

A3

()

A4

()

A5

()

A6

()

Sam

ple

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

No

04

04

04

04

04

04

14

01

62

12

43

81

51

71

94

03

24

03

95

03

63

43

01

01

21

51

71

01

51

01

11

91

73

73

01

91

1

26

52

54

24

66

53

14

04

46

85

25

54

96

35

24

84

86

25

04

56

17

07

15

76

04

44

15

04

35

46

1

36

23

74

75

06

23

54

95

56

65

06

95

26

05

65

54

16

96

06

56

03

54

74

95

05

05

25

13

86

14

7

46

84

54

04

56

44

15

35

27

35

66

65

96

95

95

15

27

36

46

97

15

23

66

66

74

15

05

75

07

34

9

56

02

22

43

15

02

73

6T4

05

4T4

24

9T4

3

51

39

37

34

12

19

10

20

38

T2

01

012

29

38

43

33

21

16

__

__

___

___

___

__

___

S

light

tai

ling

T

T

ailin

g

Sa

mp

le N

o

1

vita

min

B 1 2

vita

min

B2

3

vita

min

B6

4

folic

aci

d

5 v

itam

in B 1

2A

1 c

hlo

rofo

rm-n

-bu

tan

ol-

ace

tic a

cid

-am

mo

nia

(4

75

1

vv)

A

2 c

hlo

rofo

rm-n

-bu

tan

ol-

wa

ter-

ace

tic a

cid

-am

mo

nia

(3

50

55

05

v

v)

A3

be

nze

ne

-B

utyl

acet

ate-

n-pr

opan

ol-a

cetic

aci

d-am

mon

ia (

14

15

1 v

v)

A

4 c

arb

on

te

tra

chlo

ride

-bu

tyla

ceta

te-p

rop

ion

ic a

cid

-am

mo

nia

(3

79

3

vv)

A

5 c

arb

on

te

tra

chlo

rid

e-b

uty

lace

tate

-me

tha

no

l-a

mm

on

ia (

15

45

70

5

vv)

A

6 c

arb

on

te

tra

chlo

rid

e-b

uty

lace

tate

-pro

pio

nic

aci

d-m

eth

an

ol-

wa

ter

(23

10

53

v

v)

Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS











Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

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ovem

ber

2014

ORDER REPRINTS












Dow

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nta

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ORDER REPRINTS









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ORDER REPRINTS






ACKNOWLEDGMENTS


REFERENCES


Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

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2 N

ovem

ber

2014

ORDER REPRINTS


















Dow

nloa

ded

by [

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vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

Order now








Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

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ovem

ber

2014

ORDER REPRINTS














Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS


Tab

le 1

HR

f Val

ues

of V

itam

ins

on P

late

s Im

preg

nate

d w

ith D

iffer

ent C

once

ntra

tions

of M

nSO 4

A1

()

A2

()

A3

()

A4

()

A5

()

A6

()

Sam

ple

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

No

04

04

04

04

04

04

14

02

42

83

03

81

42

82

94

02

33

03

85

03

74

84

71

02

01

51

61

00

70

70

83

22

83

94

81

40

7

26

55

55

45

66

55

36

06

36

85

24

75

06

35

85

95

56

26

06

06

17

05

85

77

05

56

25

35

36

26

8

36

25

15

75

96

24

95

85

96

65

55

65

86

05

45

65

96

96

56

56

43

53

33

13

35

95

85

86

06

53

1

46

86

56

26

46

46

25

45

47

35

86

06

46

56

26

46

27

37

17

57

65

24

94

85

1

64

51

63

64

74

50

56

03

25

55

85

02

45

03

85

4T

32

48T

50

5

14

04

24

21

21

51

01

13

8T

33

41

43

56

36

52

45

19

41

__

__

___

___

___

__

___

S

light

tai

ling

T

T

ailin

g

Sa

mp

le N

o

1

vita

min

B 1 2

vita

min

B2

3

vita

min

B6

4

folic

aci

d

5 v

itam

in B 1

2A

1 c

hlo

rofo

rm-n

-bu

tan

ol-

ace

tic a

cid

-am

mo

nia

(4

75

1

vv)

A

2 c

hlo

rofo

rm-n

-bu

tan

ol-

wa

ter-

ace

tic a

cid

-am

mo

nia

(3

50

55

05

v

v)

A3

be

nze

ne

-B

utyl

acet

ate-

n-pr

opan

ol-a

cetic

aci

d-am

mon

ia (

14

15

1 v

v)

A

4 c

arb

on

te

tra

chlo

ride

-bu

tyla

ceta

te-p

rop

ion

ic a

cid

-am

mo

nia

(3

79

3

vv)

A

5 c

arb

on

tetr

ach

lorid

e-b

uty

lace

tate

-me

tha

no

l-a

mm

on

ia (

15

45

70

5

vv)

A

6 c

arb

on

te

tra

chlo

rid

e-b

uty

lace

tate

-pro

pio

nic

aci

d-m

eth

an

ol-

wa

ter

(23

10

53

v

v)

Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS


Tab

le 2

HR

f Val

ues

of V

itam

ins

on P

late

s Im

preg

nate

d w

ith D

iffer

ent C

once

ntra

tions

of F

eSO 4

A1

()

A2

()

A3

()

A4

()

A5

()

A6

()

Sam

ple

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

No

04

04

04

04

04

04

14

02

73

23

53

83

02

12

34

01

52

14

15

03

53

43

21

01

51

81

71

01

02

22

63

42

14

24

31

50

8

26

53

86

66

96

54

85

65

76

82

55

05

46

35

55

35

26

24

86

26

37

07

57

86

76

55

55

45

66

36

6

36

24

56

06

56

24

15

35

46

63

15

35

76

05

85

75

66

95

35

05

53

55

56

97

06

35

15

55

95

86

8

46

84

97

07

06

44

56

16

37

33

56

06

36

56

36

16

17

35

77

87

75

27

17

17

36

94

76

16

57

67

1

56

02

34

55

15

03

62

9T3

15

41

94

25

05

14

54

03

91

21

72

02

13

8T

39

48

51

50

30

51

48

19

49

__

__

___

___

___

__

___

T

Tai

ling

S

am

ple

No

1

vi

tam

in B 1

2

vita

min

B2

3 v

itam

in B

6 4

fo

lic a

cid

5

vita

min

B 12

A1

ch

loro

form

-n-b

uta

no

l-a

cetic

aci

d-a

mm

on

ia (

47

51

v

v)

A2

chl

oro

form

-n-b

uta

no

l-w

ate

r-a

cetic

aci

d-a

mm

on

ia (

35

05

50

5

vv)

A

3 b

en

zen

e-

But

ylac

etat

e-n-

prop

anol

-ace

tic a

cid-

amm

onia

(1

41

51

vv

)

A4

ca

rbo

n t

etr

ach

lorid

e-b

uty

lace

tate

-pro

pio

nic

aci

d-a

mm

on

ia (

37

93

v

v)

A5

ca

rbo

nte

tra

chlo

ride

-bu

tyla

ceta

te-m

eth

an

ol-

am

mo

nia

(1

54

57

05

v

v)

A6

ca

rbo

n t

etr

ach

lori

de

-bu

tyla

ceta

te-p

rop

ioni

c a

cid

-met

ha

no

l-w

ate

r (2

31

05

3

vv)

Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS


Tab

le 3

HR

f Val

ues

of V

itam

ins

on P

late

s Im

preg

nate

d w

ith D

iffer

ent C

once

ntra

tions

of C

oSO 4

A1

()

A2

()

A3

()

A4

()

A5

()

A6

()

Sam

ple

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

No

04

04

04

04

04

04

14

03

82

02

43

82

42

52

64

04

62

24

35

04

23

53

31

01

22

01

91

01

81

51

82

22

54

24

21

81

6

26

56

45

55

96

56

06

16

16

87

34

95

16

35

53

93

66

25

96

26

17

07

16

87

05

75

85

05

46

06

8

36

26

15

75

86

25

65

65

86

67

35

15

56

05

95

85

06

96

36

96

03

56

16

56

65

55

35

65

05

96

0

46

86

56

56

76

46

86

96

17

37

67

26

06

56

46

46

37

36

77

17

35

26

77

27

46

54

86

16

47

56

5

56

05

43

44

55

03

54

0T4

15

4T5

64

2T4

95

14

74

04

01

23

02

3T2

43

8T3

93

43

64

34

04

64

92

63

5_

__

___

___

__

___

___

_T

T

ailin

g

Sa

mp

le N

o

1

vita

min

B 1 2

vi

tam

in B

2 3

vita

min

B6

4

folic

aci

d 5

vi

tam

in B 1

2A

1 c

hlo

rofo

rm-n

-bu

tan

ol-

ace

tic a

cid

-am

mo

nia

(4

75

1

vv)

A

2 c

hlo

rofo

rm-n

-bu

tan

ol-

wa

ter-

ace

tic a

cid

-am

mo

nia

(3

50

55

05

v

v)

A3

be

nze

ne

-B

utyl

acet

ate-

n-pr

opan

ol-a

cetic

aci

d-am

mon

ia (

14

15

1 v

v)

A

4 c

arb

on

te

tra

chlo

rid

e-b

uty

lace

tate

-pro

pio

nic

aci

d-a

mm

on

ia (

37

93

v

v)

A5

ca

rbo

n

tetr

ach

lori

de

-bu

tyla

ceta

te-m

eth

an

ol-

am

mo

nia

(1

54

57

05

v

v)

A6

ca

rbo

n t

etr

ach

lori

de

-bu

tyla

ceta

te-p

rop

ioni

c a

cid

-met

ha

no

l-w

ate

r (2

31

05

3

vv)

Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS


Tab

le 4

HR

f Val

ues

of V

itam

ins

on P

late

s Im

preg

nate

d w

ith D

iffer

ent C

once

ntra

tions

of N

iSO 4

A1

()

A2

()

A3

()

A4

()

A5

()

A6

()

Sam

ple

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

No

04

04

04

04

04

04

14

02

22

83

03

80

62

42

54

03

13

83

65

04

03

23

11

01

52

82

41

02

31

51

92

72

44

22

82

61

6

26

54

75

55

86

56

35

55

76

83

46

46

06

35

23

84

66

25

26

06

17

06

96

56

65

65

65

94

26

36

5

36

25

25

25

56

25

25

65

56

61

56

56

06

05

95

04

96

96

56

76

53

56

36

66

85

45

36

34

76

76

0

46

86

55

36

26

45

95

85

97

33

06

46

36

56

45

15

57

37

27

37

35

27

57

37

66

04

76

65

27

46

1_

__

___

___

__

___

___

_T

T

ailin

g

Sa

mp

le N

o

1

vita

min

B 1 2

vi

tam

in B

2 3

vita

min

B6

4

folic

aci

d

5 v

itam

in B 1

2A

1 c

hlo

rofo

rm-n

-bu

tan

ol-

ace

tic a

cid

-am

mo

nia

(4

75

1

vv)

A

2 c

hlo

rofo

rm-n

-bu

tan

ol-

wa

ter-

ace

tic a

cid

-am

mo

nia

(3

50

55

05

v

v)

A3

be

nze

ne

-B

utyl

acet

ate-

n-pr

opan

ol-a

cetic

aci

d-am

mon

ia (

14

15

1 v

v)

A

4 c

arb

on

te

tra

chlo

ride

-bu

tyla

ceta

te-p

rop

ion

ic a

cid

-am

mo

nia

(3

79

3

vv)

A

5 c

arb

on

T

etr

ach

lori

de

-bu

tyla

ceta

te-m

eth

an

ol-

am

mo

nia

(1

54

57

05

v

v)

A6

ca

rbo

n t

etr

ach

lorid

e-b

uty

lace

tate

-pro

pio

nic

aci

d-m

eth

an

ol-

wa

ter

(23

10

53

vv

)

Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS


Tab

le 5

HR

f Val

ues

of V

itam

ins

on P

late

s Im

preg

nate

d w

ith D

iffer

ent C

once

ntra

tions

of C

uSO 4

A1

()

A2

()

A3

()

A4

()

A5

()

A6

()

Sam

ple

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

No

04

04

04

04

04

04

14

03

13

94

13

82

2T2

03

14

03

54

04

45

05

24

23

51

02

02

92

71

02

41

82

13

92

94

13

63

01

9

26

56

46

56

66

56

36

06

16

86

06

66

76

35

95

65

36

26

46

36

47

07

57

07

36

45

95

75

56

47

1

36

26

06

97

56

26

05

95

86

66

16

87

06

06

36

15

06

97

47

57

63

57

26

66

77

15

56

05

96

76

8

46

86

85

76

16

46

46

36

47

37

16

47

16

56

95

24

67

37

88

07

65

27

97

98

26

05

86

36

27

57

7

56

04

24

04

95

03

93

83

55

4T

43

45

47

51

48

36

38

12

24

26

26

38

T3

32

52

74

63

44

64

02

52

5_

__

___

___

__

___

___

_T

T

ailin

g

Sa

mp

le N

o

1

vita

min

B 1 2

vi

tam

in B

2 3

vita

min

B6

4

folic

aci

d

5 v

itam

in B 1

2A

1 c

hlo

rofo

rm-n

-bu

tan

ol-

ace

tic a

cid

-am

mo

nia

(4

75

1

vv)

A

2 c

hlo

rofo

rm-n

-bu

tan

ol-

wa

ter-

ace

tic a

cid

-am

mo

nia

(3

50

55

05

v

v)

A3

be

nze

ne

-B

utyl

acet

ate-

n-pr

opan

ol-a

cetic

aci

d-am

mon

ia (

14

15

1 v

v)

A

4 c

arb

on

te

tra

chlo

ride

-bu

tyla

ceta

te-p

rop

ion

ic a

cid

-am

mo

nia

(3

79

3

vv)

A

5 c

arb

on

T

etr

ach

lori

de

-bu

tyla

ceta

te-m

eth

an

ol-

am

mo

nia

(1

54

57

05

v

v)

A6

ca

rbo

n t

etr

ach

lorid

e-b

uty

lace

tate

-pro

pio

nic

aci

d-m

eth

an

ol-

wa

ter

(23

10

53

vv

)

Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS


Tab

le 6

HR

f Val

ues

of V

itam

ins

on P

late

s Im

preg

nate

d w

ith D

iffer

ent C

once

ntra

tions

of Z

nSO 4

A1

()

A2

()

A3

()

A4

()

A5

()

A6

()

Sam

ple

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

No

04

04

04

04

04

04

14

03

93

84

03

81

8T2

12

54

02

24

24

15

03

03

53

21

01

82

72

61

03

41

21

43

62

33

92

62

71

0

26

56

06

16

36

56

54

95

36

84

06

76

16

34

44

85

16

25

86

16

27

07

85

56

16

25

05

74

46

36

9

36

25

76

67

26

25

55

76

46

64

36

85

86

04

85

54

76

96

47

06

53

55

66

06

76

96

35

93

15

86

4

46

86

65

35

56

46

06

06

17

36

36

56

56

55

45

55

47

36

67

17

35

26

07

17

95

25

86

05

27

05

8

56

04

73

24

35

03

23

32

95

4T

37

48

46

51

34

39

36

12

22

24

25

38

T4

22

12

24

12

74

53

42

31

8_

__

___

___

__

___

___

_T

T

ailin

g

Sa

mp

le N

o

1

vita

min

B 1 2

vi

tam

in B

2 3

vita

min

B6

4

folic

aci

d

5 v

itam

in B 1

2A

1 c

hlo

rofo

rm-n

-bu

tan

ol-

ace

tic a

cid

-am

mo

nia

(4

75

1

vv)

A

2 c

hlo

rofo

rm-n

-bu

tan

ol-

wa

ter-

ace

tic a

cid

-am

mo

nia

(3

50

55

05

v

v)

A3

be

nze

ne

-B

utyl

acet

ate-

n-pr

opan

ol-a

cetic

aci

d-am

mon

ia (

14

15

1 v

v)

A

4 c

arb

on

te

tra

chlo

ride

-bu

tyla

ceta

te-p

rop

ion

ic a

cid

-am

mo

nia

(3

79

3

vv)

A

5 c

arb

on

te

tra

chlo

rid

e-b

uty

lace

tate

-me

tha

no

l-a

mm

on

ia (

15

45

70

5

vv)

A

6 c

arb

on

te

tra

chlo

rid

e-b

uty

lace

tate

-pro

pio

nic

aci

d-m

eth

an

ol-

wa

ter

(23

10

53

v

v)

Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS


Tab

le 7

HR

f Val

ues

of V

itam

ins

on P

late

s Im

preg

nate

d w

ith D

iffer

ent C

once

ntra

tions

of C

dSO 4

A1

()

A2

()

A3

()

A4

()

A5

()

A6

()

Sam

ple

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

No

04

04

04

04

04

04

14

01

72

12

53

81

11

31

54

01

74

24

15

03

63

63

01

02

33

13

01

02

41

11

52

11

43

72

62

91

1

26

54

54

64

96

55

34

84

76

84

86

65

06

35

54

74

96

24

76

56

47

07

05

96

74

74

35

14

76

66

5

36

24

94

95

16

24

34

95

36

65

96

55

16

05

85

34

25

95

27

26

43

56

35

56

15

04

95

34

06

35

4

46

86

74

16

96

45

05

05

77

36

86

96

16

56

54

84

67

36

07

67

65

26

57

07

24

15

55

84

47

46

0

56

02

32

63

35

01

42

62

55

4T

14

46

43

51

31

35

35

12

39

24

24

38

T3

01

71

93

02

44

53

31

91

8_

__

___

___

__

___

___

_T

T

ailin

g

Sa

mp

le N

o

1

vita

min

B 1 2

vi

tam

in B

2 3

vita

min

B6

4

folic

aci

d

5 v

itam

in B 1

2A

1 c

hlo

rofo

rm-n

-bu

tan

ol-

ace

tic a

cid

-am

mo

nia

(4

75

1

vv)

A

2 c

hlo

rofo

rm-n

-bu

tan

ol-

wa

ter-

ace

tic a

cid

-am

mo

nia

(3

50

55

05

v

v)

A3

be

nze

ne

-B

utyl

acet

ate-

n-pr

opan

ol-a

cetic

aci

d-am

mon

ia (

14

15

1 v

v)

A

4 c

arb

on

te

tra

chlo

ride

-bu

tyla

ceta

te-p

rop

ion

ic a

cid

-am

mo

nia

(3

79

3

vv)

A

5 c

arb

on

te

tra

chlo

rid

e-b

uty

lace

tate

-me

tha

no

l-a

mm

on

ia (

15

45

70

5

vv)

A

6 c

arb

on

te

tra

chlo

rid

e-b

uty

lace

tate

-pro

pio

nic

aci

d-m

eth

an

ol-

wa

ter

(23

10

53

v

v)

Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS


Tab

le 8

HR

f Val

ues

of V

itam

ins

on P

late

s Im

preg

nate

d w

ith D

iffer

ent C

once

ntra

tions

of H

gSO 4

A1

()

A2

()

A3

()

A4

()

A5

()

A6

()

Sam

ple

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

No

04

04

04

04

04

04

14

01

62

12

43

81

51

71

94

03

24

03

95

03

63

43

01

01

21

51

71

01

51

01

11

91

73

73

01

91

1

26

52

54

24

66

53

14

04

46

85

25

54

96

35

24

84

86

25

04

56

17

07

15

76

04

44

15

04

35

46

1

36

23

74

75

06

23

54

95

56

65

06

95

26

05

65

54

16

96

06

56

03

54

74

95

05

05

25

13

86

14

7

46

84

54

04

56

44

15

35

27

35

66

65

96

95

95

15

27

36

46

97

15

23

66

66

74

15

05

75

07

34

9

56

02

22

43

15

02

73

6T4

05

4T4

24

9T4

3

51

39

37

34

12

19

10

20

38

T2

01

012

29

38

43

33

21

16

__

__

___

___

___

__

___

S

light

tai

ling

T

T

ailin

g

Sa

mp

le N

o

1

vita

min

B 1 2

vita

min

B2

3

vita

min

B6

4

folic

aci

d

5 v

itam

in B 1

2A

1 c

hlo

rofo

rm-n

-bu

tan

ol-

ace

tic a

cid

-am

mo

nia

(4

75

1

vv)

A

2 c

hlo

rofo

rm-n

-bu

tan

ol-

wa

ter-

ace

tic a

cid

-am

mo

nia

(3

50

55

05

v

v)

A3

be

nze

ne

-B

utyl

acet

ate-

n-pr

opan

ol-a

cetic

aci

d-am

mon

ia (

14

15

1 v

v)

A

4 c

arb

on

te

tra

chlo

ride

-bu

tyla

ceta

te-p

rop

ion

ic a

cid

-am

mo

nia

(3

79

3

vv)

A

5 c

arb

on

te

tra

chlo

rid

e-b

uty

lace

tate

-me

tha

no

l-a

mm

on

ia (

15

45

70

5

vv)

A

6 c

arb

on

te

tra

chlo

rid

e-b

uty

lace

tate

-pro

pio

nic

aci

d-m

eth

an

ol-

wa

ter

(23

10

53

v

v)

Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS











Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS












Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS









Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS









Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS






ACKNOWLEDGMENTS


REFERENCES


Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS


















Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

Order now








Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS


Tab

le 1

HR

f Val

ues

of V

itam

ins

on P

late

s Im

preg

nate

d w

ith D

iffer

ent C

once

ntra

tions

of M

nSO 4

A1

()

A2

()

A3

()

A4

()

A5

()

A6

()

Sam

ple

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

No

04

04

04

04

04

04

14

02

42

83

03

81

42

82

94

02

33

03

85

03

74

84

71

02

01

51

61

00

70

70

83

22

83

94

81

40

7

26

55

55

45

66

55

36

06

36

85

24

75

06

35

85

95

56

26

06

06

17

05

85

77

05

56

25

35

36

26

8

36

25

15

75

96

24

95

85

96

65

55

65

86

05

45

65

96

96

56

56

43

53

33

13

35

95

85

86

06

53

1

46

86

56

26

46

46

25

45

47

35

86

06

46

56

26

46

27

37

17

57

65

24

94

85

1

64

51

63

64

74

50

56

03

25

55

85

02

45

03

85

4T

32

48T

50

5

14

04

24

21

21

51

01

13

8T

33

41

43

56

36

52

45

19

41

__

__

___

___

___

__

___

S

light

tai

ling

T

T

ailin

g

Sa

mp

le N

o

1

vita

min

B 1 2

vita

min

B2

3

vita

min

B6

4

folic

aci

d

5 v

itam

in B 1

2A

1 c

hlo

rofo

rm-n

-bu

tan

ol-

ace

tic a

cid

-am

mo

nia

(4

75

1

vv)

A

2 c

hlo

rofo

rm-n

-bu

tan

ol-

wa

ter-

ace

tic a

cid

-am

mo

nia

(3

50

55

05

v

v)

A3

be

nze

ne

-B

utyl

acet

ate-

n-pr

opan

ol-a

cetic

aci

d-am

mon

ia (

14

15

1 v

v)

A

4 c

arb

on

te

tra

chlo

ride

-bu

tyla

ceta

te-p

rop

ion

ic a

cid

-am

mo

nia

(3

79

3

vv)

A

5 c

arb

on

tetr

ach

lorid

e-b

uty

lace

tate

-me

tha

no

l-a

mm

on

ia (

15

45

70

5

vv)

A

6 c

arb

on

te

tra

chlo

rid

e-b

uty

lace

tate

-pro

pio

nic

aci

d-m

eth

an

ol-

wa

ter

(23

10

53

v

v)

Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS


Tab

le 2

HR

f Val

ues

of V

itam

ins

on P

late

s Im

preg

nate

d w

ith D

iffer

ent C

once

ntra

tions

of F

eSO 4

A1

()

A2

()

A3

()

A4

()

A5

()

A6

()

Sam

ple

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

No

04

04

04

04

04

04

14

02

73

23

53

83

02

12

34

01

52

14

15

03

53

43

21

01

51

81

71

01

02

22

63

42

14

24

31

50

8

26

53

86

66

96

54

85

65

76

82

55

05

46

35

55

35

26

24

86

26

37

07

57

86

76

55

55

45

66

36

6

36

24

56

06

56

24

15

35

46

63

15

35

76

05

85

75

66

95

35

05

53

55

56

97

06

35

15

55

95

86

8

46

84

97

07

06

44

56

16

37

33

56

06

36

56

36

16

17

35

77

87

75

27

17

17

36

94

76

16

57

67

1

56

02

34

55

15

03

62

9T3

15

41

94

25

05

14

54

03

91

21

72

02

13

8T

39

48

51

50

30

51

48

19

49

__

__

___

___

___

__

___

T

Tai

ling

S

am

ple

No

1

vi

tam

in B 1

2

vita

min

B2

3 v

itam

in B

6 4

fo

lic a

cid

5

vita

min

B 12

A1

ch

loro

form

-n-b

uta

no

l-a

cetic

aci

d-a

mm

on

ia (

47

51

v

v)

A2

chl

oro

form

-n-b

uta

no

l-w

ate

r-a

cetic

aci

d-a

mm

on

ia (

35

05

50

5

vv)

A

3 b

en

zen

e-

But

ylac

etat

e-n-

prop

anol

-ace

tic a

cid-

amm

onia

(1

41

51

vv

)

A4

ca

rbo

n t

etr

ach

lorid

e-b

uty

lace

tate

-pro

pio

nic

aci

d-a

mm

on

ia (

37

93

v

v)

A5

ca

rbo

nte

tra

chlo

ride

-bu

tyla

ceta

te-m

eth

an

ol-

am

mo

nia

(1

54

57

05

v

v)

A6

ca

rbo

n t

etr

ach

lori

de

-bu

tyla

ceta

te-p

rop

ioni

c a

cid

-met

ha

no

l-w

ate

r (2

31

05

3

vv)

Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS


Tab

le 3

HR

f Val

ues

of V

itam

ins

on P

late

s Im

preg

nate

d w

ith D

iffer

ent C

once

ntra

tions

of C

oSO 4

A1

()

A2

()

A3

()

A4

()

A5

()

A6

()

Sam

ple

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

No

04

04

04

04

04

04

14

03

82

02

43

82

42

52

64

04

62

24

35

04

23

53

31

01

22

01

91

01

81

51

82

22

54

24

21

81

6

26

56

45

55

96

56

06

16

16

87

34

95

16

35

53

93

66

25

96

26

17

07

16

87

05

75

85

05

46

06

8

36

26

15

75

86

25

65

65

86

67

35

15

56

05

95

85

06

96

36

96

03

56

16

56

65

55

35

65

05

96

0

46

86

56

56

76

46

86

96

17

37

67

26

06

56

46

46

37

36

77

17

35

26

77

27

46

54

86

16

47

56

5

56

05

43

44

55

03

54

0T4

15

4T5

64

2T4

95

14

74

04

01

23

02

3T2

43

8T3

93

43

64

34

04

64

92

63

5_

__

___

___

__

___

___

_T

T

ailin

g

Sa

mp

le N

o

1

vita

min

B 1 2

vi

tam

in B

2 3

vita

min

B6

4

folic

aci

d 5

vi

tam

in B 1

2A

1 c

hlo

rofo

rm-n

-bu

tan

ol-

ace

tic a

cid

-am

mo

nia

(4

75

1

vv)

A

2 c

hlo

rofo

rm-n

-bu

tan

ol-

wa

ter-

ace

tic a

cid

-am

mo

nia

(3

50

55

05

v

v)

A3

be

nze

ne

-B

utyl

acet

ate-

n-pr

opan

ol-a

cetic

aci

d-am

mon

ia (

14

15

1 v

v)

A

4 c

arb

on

te

tra

chlo

rid

e-b

uty

lace

tate

-pro

pio

nic

aci

d-a

mm

on

ia (

37

93

v

v)

A5

ca

rbo

n

tetr

ach

lori

de

-bu

tyla

ceta

te-m

eth

an

ol-

am

mo

nia

(1

54

57

05

v

v)

A6

ca

rbo

n t

etr

ach

lori

de

-bu

tyla

ceta

te-p

rop

ioni

c a

cid

-met

ha

no

l-w

ate

r (2

31

05

3

vv)

Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS


Tab

le 4

HR

f Val

ues

of V

itam

ins

on P

late

s Im

preg

nate

d w

ith D

iffer

ent C

once

ntra

tions

of N

iSO 4

A1

()

A2

()

A3

()

A4

()

A5

()

A6

()

Sam

ple

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

No

04

04

04

04

04

04

14

02

22

83

03

80

62

42

54

03

13

83

65

04

03

23

11

01

52

82

41

02

31

51

92

72

44

22

82

61

6

26

54

75

55

86

56

35

55

76

83

46

46

06

35

23

84

66

25

26

06

17

06

96

56

65

65

65

94

26

36

5

36

25

25

25

56

25

25

65

56

61

56

56

06

05

95

04

96

96

56

76

53

56

36

66

85

45

36

34

76

76

0

46

86

55

36

26

45

95

85

97

33

06

46

36

56

45

15

57

37

27

37

35

27

57

37

66

04

76

65

27

46

1_

__

___

___

__

___

___

_T

T

ailin

g

Sa

mp

le N

o

1

vita

min

B 1 2

vi

tam

in B

2 3

vita

min

B6

4

folic

aci

d

5 v

itam

in B 1

2A

1 c

hlo

rofo

rm-n

-bu

tan

ol-

ace

tic a

cid

-am

mo

nia

(4

75

1

vv)

A

2 c

hlo

rofo

rm-n

-bu

tan

ol-

wa

ter-

ace

tic a

cid

-am

mo

nia

(3

50

55

05

v

v)

A3

be

nze

ne

-B

utyl

acet

ate-

n-pr

opan

ol-a

cetic

aci

d-am

mon

ia (

14

15

1 v

v)

A

4 c

arb

on

te

tra

chlo

ride

-bu

tyla

ceta

te-p

rop

ion

ic a

cid

-am

mo

nia

(3

79

3

vv)

A

5 c

arb

on

T

etr

ach

lori

de

-bu

tyla

ceta

te-m

eth

an

ol-

am

mo

nia

(1

54

57

05

v

v)

A6

ca

rbo

n t

etr

ach

lorid

e-b

uty

lace

tate

-pro

pio

nic

aci

d-m

eth

an

ol-

wa

ter

(23

10

53

vv

)

Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS


Tab

le 5

HR

f Val

ues

of V

itam

ins

on P

late

s Im

preg

nate

d w

ith D

iffer

ent C

once

ntra

tions

of C

uSO 4

A1

()

A2

()

A3

()

A4

()

A5

()

A6

()

Sam

ple

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

No

04

04

04

04

04

04

14

03

13

94

13

82

2T2

03

14

03

54

04

45

05

24

23

51

02

02

92

71

02

41

82

13

92

94

13

63

01

9

26

56

46

56

66

56

36

06

16

86

06

66

76

35

95

65

36

26

46

36

47

07

57

07

36

45

95

75

56

47

1

36

26

06

97

56

26

05

95

86

66

16

87

06

06

36

15

06

97

47

57

63

57

26

66

77

15

56

05

96

76

8

46

86

85

76

16

46

46

36

47

37

16

47

16

56

95

24

67

37

88

07

65

27

97

98

26

05

86

36

27

57

7

56

04

24

04

95

03

93

83

55

4T

43

45

47

51

48

36

38

12

24

26

26

38

T3

32

52

74

63

44

64

02

52

5_

__

___

___

__

___

___

_T

T

ailin

g

Sa

mp

le N

o

1

vita

min

B 1 2

vi

tam

in B

2 3

vita

min

B6

4

folic

aci

d

5 v

itam

in B 1

2A

1 c

hlo

rofo

rm-n

-bu

tan

ol-

ace

tic a

cid

-am

mo

nia

(4

75

1

vv)

A

2 c

hlo

rofo

rm-n

-bu

tan

ol-

wa

ter-

ace

tic a

cid

-am

mo

nia

(3

50

55

05

v

v)

A3

be

nze

ne

-B

utyl

acet

ate-

n-pr

opan

ol-a

cetic

aci

d-am

mon

ia (

14

15

1 v

v)

A

4 c

arb

on

te

tra

chlo

ride

-bu

tyla

ceta

te-p

rop

ion

ic a

cid

-am

mo

nia

(3

79

3

vv)

A

5 c

arb

on

T

etr

ach

lori

de

-bu

tyla

ceta

te-m

eth

an

ol-

am

mo

nia

(1

54

57

05

v

v)

A6

ca

rbo

n t

etr

ach

lorid

e-b

uty

lace

tate

-pro

pio

nic

aci

d-m

eth

an

ol-

wa

ter

(23

10

53

vv

)

Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS


Tab

le 6

HR

f Val

ues

of V

itam

ins

on P

late

s Im

preg

nate

d w

ith D

iffer

ent C

once

ntra

tions

of Z

nSO 4

A1

()

A2

()

A3

()

A4

()

A5

()

A6

()

Sam

ple

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

No

04

04

04

04

04

04

14

03

93

84

03

81

8T2

12

54

02

24

24

15

03

03

53

21

01

82

72

61

03

41

21

43

62

33

92

62

71

0

26

56

06

16

36

56

54

95

36

84

06

76

16

34

44

85

16

25

86

16

27

07

85

56

16

25

05

74

46

36

9

36

25

76

67

26

25

55

76

46

64

36

85

86

04

85

54

76

96

47

06

53

55

66

06

76

96

35

93

15

86

4

46

86

65

35

56

46

06

06

17

36

36

56

56

55

45

55

47

36

67

17

35

26

07

17

95

25

86

05

27

05

8

56

04

73

24

35

03

23

32

95

4T

37

48

46

51

34

39

36

12

22

24

25

38

T4

22

12

24

12

74

53

42

31

8_

__

___

___

__

___

___

_T

T

ailin

g

Sa

mp

le N

o

1

vita

min

B 1 2

vi

tam

in B

2 3

vita

min

B6

4

folic

aci

d

5 v

itam

in B 1

2A

1 c

hlo

rofo

rm-n

-bu

tan

ol-

ace

tic a

cid

-am

mo

nia

(4

75

1

vv)

A

2 c

hlo

rofo

rm-n

-bu

tan

ol-

wa

ter-

ace

tic a

cid

-am

mo

nia

(3

50

55

05

v

v)

A3

be

nze

ne

-B

utyl

acet

ate-

n-pr

opan

ol-a

cetic

aci

d-am

mon

ia (

14

15

1 v

v)

A

4 c

arb

on

te

tra

chlo

ride

-bu

tyla

ceta

te-p

rop

ion

ic a

cid

-am

mo

nia

(3

79

3

vv)

A

5 c

arb

on

te

tra

chlo

rid

e-b

uty

lace

tate

-me

tha

no

l-a

mm

on

ia (

15

45

70

5

vv)

A

6 c

arb

on

te

tra

chlo

rid

e-b

uty

lace

tate

-pro

pio

nic

aci

d-m

eth

an

ol-

wa

ter

(23

10

53

v

v)

Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS


Tab

le 7

HR

f Val

ues

of V

itam

ins

on P

late

s Im

preg

nate

d w

ith D

iffer

ent C

once

ntra

tions

of C

dSO 4

A1

()

A2

()

A3

()

A4

()

A5

()

A6

()

Sam

ple

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

No

04

04

04

04

04

04

14

01

72

12

53

81

11

31

54

01

74

24

15

03

63

63

01

02

33

13

01

02

41

11

52

11

43

72

62

91

1

26

54

54

64

96

55

34

84

76

84

86

65

06

35

54

74

96

24

76

56

47

07

05

96

74

74

35

14

76

66

5

36

24

94

95

16

24

34

95

36

65

96

55

16

05

85

34

25

95

27

26

43

56

35

56

15

04

95

34

06

35

4

46

86

74

16

96

45

05

05

77

36

86

96

16

56

54

84

67

36

07

67

65

26

57

07

24

15

55

84

47

46

0

56

02

32

63

35

01

42

62

55

4T

14

46

43

51

31

35

35

12

39

24

24

38

T3

01

71

93

02

44

53

31

91

8_

__

___

___

__

___

___

_T

T

ailin

g

Sa

mp

le N

o

1

vita

min

B 1 2

vi

tam

in B

2 3

vita

min

B6

4

folic

aci

d

5 v

itam

in B 1

2A

1 c

hlo

rofo

rm-n

-bu

tan

ol-

ace

tic a

cid

-am

mo

nia

(4

75

1

vv)

A

2 c

hlo

rofo

rm-n

-bu

tan

ol-

wa

ter-

ace

tic a

cid

-am

mo

nia

(3

50

55

05

v

v)

A3

be

nze

ne

-B

utyl

acet

ate-

n-pr

opan

ol-a

cetic

aci

d-am

mon

ia (

14

15

1 v

v)

A

4 c

arb

on

te

tra

chlo

ride

-bu

tyla

ceta

te-p

rop

ion

ic a

cid

-am

mo

nia

(3

79

3

vv)

A

5 c

arb

on

te

tra

chlo

rid

e-b

uty

lace

tate

-me

tha

no

l-a

mm

on

ia (

15

45

70

5

vv)

A

6 c

arb

on

te

tra

chlo

rid

e-b

uty

lace

tate

-pro

pio

nic

aci

d-m

eth

an

ol-

wa

ter

(23

10

53

v

v)

Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS


Tab

le 8

HR

f Val

ues

of V

itam

ins

on P

late

s Im

preg

nate

d w

ith D

iffer

ent C

once

ntra

tions

of H

gSO 4

A1

()

A2

()

A3

()

A4

()

A5

()

A6

()

Sam

ple

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

No

04

04

04

04

04

04

14

01

62

12

43

81

51

71

94

03

24

03

95

03

63

43

01

01

21

51

71

01

51

01

11

91

73

73

01

91

1

26

52

54

24

66

53

14

04

46

85

25

54

96

35

24

84

86

25

04

56

17

07

15

76

04

44

15

04

35

46

1

36

23

74

75

06

23

54

95

56

65

06

95

26

05

65

54

16

96

06

56

03

54

74

95

05

05

25

13

86

14

7

46

84

54

04

56

44

15

35

27

35

66

65

96

95

95

15

27

36

46

97

15

23

66

66

74

15

05

75

07

34

9

56

02

22

43

15

02

73

6T4

05

4T4

24

9T4

3

51

39

37

34

12

19

10

20

38

T2

01

012

29

38

43

33

21

16

__

__

___

___

___

__

___

S

light

tai

ling

T

T

ailin

g

Sa

mp

le N

o

1

vita

min

B 1 2

vita

min

B2

3

vita

min

B6

4

folic

aci

d

5 v

itam

in B 1

2A

1 c

hlo

rofo

rm-n

-bu

tan

ol-

ace

tic a

cid

-am

mo

nia

(4

75

1

vv)

A

2 c

hlo

rofo

rm-n

-bu

tan

ol-

wa

ter-

ace

tic a

cid

-am

mo

nia

(3

50

55

05

v

v)

A3

be

nze

ne

-B

utyl

acet

ate-

n-pr

opan

ol-a

cetic

aci

d-am

mon

ia (

14

15

1 v

v)

A

4 c

arb

on

te

tra

chlo

ride

-bu

tyla

ceta

te-p

rop

ion

ic a

cid

-am

mo

nia

(3

79

3

vv)

A

5 c

arb

on

te

tra

chlo

rid

e-b

uty

lace

tate

-me

tha

no

l-a

mm

on

ia (

15

45

70

5

vv)

A

6 c

arb

on

te

tra

chlo

rid

e-b

uty

lace

tate

-pro

pio

nic

aci

d-m

eth

an

ol-

wa

ter

(23

10

53

v

v)

Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS











Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS












Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS









Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS









Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS






ACKNOWLEDGMENTS


REFERENCES


Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS


















Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

Order now








Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS


Tab

le 2

HR

f Val

ues

of V

itam

ins

on P

late

s Im

preg

nate

d w

ith D

iffer

ent C

once

ntra

tions

of F

eSO 4

A1

()

A2

()

A3

()

A4

()

A5

()

A6

()

Sam

ple

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

No

04

04

04

04

04

04

14

02

73

23

53

83

02

12

34

01

52

14

15

03

53

43

21

01

51

81

71

01

02

22

63

42

14

24

31

50

8

26

53

86

66

96

54

85

65

76

82

55

05

46

35

55

35

26

24

86

26

37

07

57

86

76

55

55

45

66

36

6

36

24

56

06

56

24

15

35

46

63

15

35

76

05

85

75

66

95

35

05

53

55

56

97

06

35

15

55

95

86

8

46

84

97

07

06

44

56

16

37

33

56

06

36

56

36

16

17

35

77

87

75

27

17

17

36

94

76

16

57

67

1

56

02

34

55

15

03

62

9T3

15

41

94

25

05

14

54

03

91

21

72

02

13

8T

39

48

51

50

30

51

48

19

49

__

__

___

___

___

__

___

T

Tai

ling

S

am

ple

No

1

vi

tam

in B 1

2

vita

min

B2

3 v

itam

in B

6 4

fo

lic a

cid

5

vita

min

B 12

A1

ch

loro

form

-n-b

uta

no

l-a

cetic

aci

d-a

mm

on

ia (

47

51

v

v)

A2

chl

oro

form

-n-b

uta

no

l-w

ate

r-a

cetic

aci

d-a

mm

on

ia (

35

05

50

5

vv)

A

3 b

en

zen

e-

But

ylac

etat

e-n-

prop

anol

-ace

tic a

cid-

amm

onia

(1

41

51

vv

)

A4

ca

rbo

n t

etr

ach

lorid

e-b

uty

lace

tate

-pro

pio

nic

aci

d-a

mm

on

ia (

37

93

v

v)

A5

ca

rbo

nte

tra

chlo

ride

-bu

tyla

ceta

te-m

eth

an

ol-

am

mo

nia

(1

54

57

05

v

v)

A6

ca

rbo

n t

etr

ach

lori

de

-bu

tyla

ceta

te-p

rop

ioni

c a

cid

-met

ha

no

l-w

ate

r (2

31

05

3

vv)

Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS


Tab

le 3

HR

f Val

ues

of V

itam

ins

on P

late

s Im

preg

nate

d w

ith D

iffer

ent C

once

ntra

tions

of C

oSO 4

A1

()

A2

()

A3

()

A4

()

A5

()

A6

()

Sam

ple

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

No

04

04

04

04

04

04

14

03

82

02

43

82

42

52

64

04

62

24

35

04

23

53

31

01

22

01

91

01

81

51

82

22

54

24

21

81

6

26

56

45

55

96

56

06

16

16

87

34

95

16

35

53

93

66

25

96

26

17

07

16

87

05

75

85

05

46

06

8

36

26

15

75

86

25

65

65

86

67

35

15

56

05

95

85

06

96

36

96

03

56

16

56

65

55

35

65

05

96

0

46

86

56

56

76

46

86

96

17

37

67

26

06

56

46

46

37

36

77

17

35

26

77

27

46

54

86

16

47

56

5

56

05

43

44

55

03

54

0T4

15

4T5

64

2T4

95

14

74

04

01

23

02

3T2

43

8T3

93

43

64

34

04

64

92

63

5_

__

___

___

__

___

___

_T

T

ailin

g

Sa

mp

le N

o

1

vita

min

B 1 2

vi

tam

in B

2 3

vita

min

B6

4

folic

aci

d 5

vi

tam

in B 1

2A

1 c

hlo

rofo

rm-n

-bu

tan

ol-

ace

tic a

cid

-am

mo

nia

(4

75

1

vv)

A

2 c

hlo

rofo

rm-n

-bu

tan

ol-

wa

ter-

ace

tic a

cid

-am

mo

nia

(3

50

55

05

v

v)

A3

be

nze

ne

-B

utyl

acet

ate-

n-pr

opan

ol-a

cetic

aci

d-am

mon

ia (

14

15

1 v

v)

A

4 c

arb

on

te

tra

chlo

rid

e-b

uty

lace

tate

-pro

pio

nic

aci

d-a

mm

on

ia (

37

93

v

v)

A5

ca

rbo

n

tetr

ach

lori

de

-bu

tyla

ceta

te-m

eth

an

ol-

am

mo

nia

(1

54

57

05

v

v)

A6

ca

rbo

n t

etr

ach

lori

de

-bu

tyla

ceta

te-p

rop

ioni

c a

cid

-met

ha

no

l-w

ate

r (2

31

05

3

vv)

Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS


Tab

le 4

HR

f Val

ues

of V

itam

ins

on P

late

s Im

preg

nate

d w

ith D

iffer

ent C

once

ntra

tions

of N

iSO 4

A1

()

A2

()

A3

()

A4

()

A5

()

A6

()

Sam

ple

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

No

04

04

04

04

04

04

14

02

22

83

03

80

62

42

54

03

13

83

65

04

03

23

11

01

52

82

41

02

31

51

92

72

44

22

82

61

6

26

54

75

55

86

56

35

55

76

83

46

46

06

35

23

84

66

25

26

06

17

06

96

56

65

65

65

94

26

36

5

36

25

25

25

56

25

25

65

56

61

56

56

06

05

95

04

96

96

56

76

53

56

36

66

85

45

36

34

76

76

0

46

86

55

36

26

45

95

85

97

33

06

46

36

56

45

15

57

37

27

37

35

27

57

37

66

04

76

65

27

46

1_

__

___

___

__

___

___

_T

T

ailin

g

Sa

mp

le N

o

1

vita

min

B 1 2

vi

tam

in B

2 3

vita

min

B6

4

folic

aci

d

5 v

itam

in B 1

2A

1 c

hlo

rofo

rm-n

-bu

tan

ol-

ace

tic a

cid

-am

mo

nia

(4

75

1

vv)

A

2 c

hlo

rofo

rm-n

-bu

tan

ol-

wa

ter-

ace

tic a

cid

-am

mo

nia

(3

50

55

05

v

v)

A3

be

nze

ne

-B

utyl

acet

ate-

n-pr

opan

ol-a

cetic

aci

d-am

mon

ia (

14

15

1 v

v)

A

4 c

arb

on

te

tra

chlo

ride

-bu

tyla

ceta

te-p

rop

ion

ic a

cid

-am

mo

nia

(3

79

3

vv)

A

5 c

arb

on

T

etr

ach

lori

de

-bu

tyla

ceta

te-m

eth

an

ol-

am

mo

nia

(1

54

57

05

v

v)

A6

ca

rbo

n t

etr

ach

lorid

e-b

uty

lace

tate

-pro

pio

nic

aci

d-m

eth

an

ol-

wa

ter

(23

10

53

vv

)

Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS


Tab

le 5

HR

f Val

ues

of V

itam

ins

on P

late

s Im

preg

nate

d w

ith D

iffer

ent C

once

ntra

tions

of C

uSO 4

A1

()

A2

()

A3

()

A4

()

A5

()

A6

()

Sam

ple

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

No

04

04

04

04

04

04

14

03

13

94

13

82

2T2

03

14

03

54

04

45

05

24

23

51

02

02

92

71

02

41

82

13

92

94

13

63

01

9

26

56

46

56

66

56

36

06

16

86

06

66

76

35

95

65

36

26

46

36

47

07

57

07

36

45

95

75

56

47

1

36

26

06

97

56

26

05

95

86

66

16

87

06

06

36

15

06

97

47

57

63

57

26

66

77

15

56

05

96

76

8

46

86

85

76

16

46

46

36

47

37

16

47

16

56

95

24

67

37

88

07

65

27

97

98

26

05

86

36

27

57

7

56

04

24

04

95

03

93

83

55

4T

43

45

47

51

48

36

38

12

24

26

26

38

T3

32

52

74

63

44

64

02

52

5_

__

___

___

__

___

___

_T

T

ailin

g

Sa

mp

le N

o

1

vita

min

B 1 2

vi

tam

in B

2 3

vita

min

B6

4

folic

aci

d

5 v

itam

in B 1

2A

1 c

hlo

rofo

rm-n

-bu

tan

ol-

ace

tic a

cid

-am

mo

nia

(4

75

1

vv)

A

2 c

hlo

rofo

rm-n

-bu

tan

ol-

wa

ter-

ace

tic a

cid

-am

mo

nia

(3

50

55

05

v

v)

A3

be

nze

ne

-B

utyl

acet

ate-

n-pr

opan

ol-a

cetic

aci

d-am

mon

ia (

14

15

1 v

v)

A

4 c

arb

on

te

tra

chlo

ride

-bu

tyla

ceta

te-p

rop

ion

ic a

cid

-am

mo

nia

(3

79

3

vv)

A

5 c

arb

on

T

etr

ach

lori

de

-bu

tyla

ceta

te-m

eth

an

ol-

am

mo

nia

(1

54

57

05

v

v)

A6

ca

rbo

n t

etr

ach

lorid

e-b

uty

lace

tate

-pro

pio

nic

aci

d-m

eth

an

ol-

wa

ter

(23

10

53

vv

)

Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS


Tab

le 6

HR

f Val

ues

of V

itam

ins

on P

late

s Im

preg

nate

d w

ith D

iffer

ent C

once

ntra

tions

of Z

nSO 4

A1

()

A2

()

A3

()

A4

()

A5

()

A6

()

Sam

ple

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

No

04

04

04

04

04

04

14

03

93

84

03

81

8T2

12

54

02

24

24

15

03

03

53

21

01

82

72

61

03

41

21

43

62

33

92

62

71

0

26

56

06

16

36

56

54

95

36

84

06

76

16

34

44

85

16

25

86

16

27

07

85

56

16

25

05

74

46

36

9

36

25

76

67

26

25

55

76

46

64

36

85

86

04

85

54

76

96

47

06

53

55

66

06

76

96

35

93

15

86

4

46

86

65

35

56

46

06

06

17

36

36

56

56

55

45

55

47

36

67

17

35

26

07

17

95

25

86

05

27

05

8

56

04

73

24

35

03

23

32

95

4T

37

48

46

51

34

39

36

12

22

24

25

38

T4

22

12

24

12

74

53

42

31

8_

__

___

___

__

___

___

_T

T

ailin

g

Sa

mp

le N

o

1

vita

min

B 1 2

vi

tam

in B

2 3

vita

min

B6

4

folic

aci

d

5 v

itam

in B 1

2A

1 c

hlo

rofo

rm-n

-bu

tan

ol-

ace

tic a

cid

-am

mo

nia

(4

75

1

vv)

A

2 c

hlo

rofo

rm-n

-bu

tan

ol-

wa

ter-

ace

tic a

cid

-am

mo

nia

(3

50

55

05

v

v)

A3

be

nze

ne

-B

utyl

acet

ate-

n-pr

opan

ol-a

cetic

aci

d-am

mon

ia (

14

15

1 v

v)

A

4 c

arb

on

te

tra

chlo

ride

-bu

tyla

ceta

te-p

rop

ion

ic a

cid

-am

mo

nia

(3

79

3

vv)

A

5 c

arb

on

te

tra

chlo

rid

e-b

uty

lace

tate

-me

tha

no

l-a

mm

on

ia (

15

45

70

5

vv)

A

6 c

arb

on

te

tra

chlo

rid

e-b

uty

lace

tate

-pro

pio

nic

aci

d-m

eth

an

ol-

wa

ter

(23

10

53

v

v)

Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS


Tab

le 7

HR

f Val

ues

of V

itam

ins

on P

late

s Im

preg

nate

d w

ith D

iffer

ent C

once

ntra

tions

of C

dSO 4

A1

()

A2

()

A3

()

A4

()

A5

()

A6

()

Sam

ple

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

No

04

04

04

04

04

04

14

01

72

12

53

81

11

31

54

01

74

24

15

03

63

63

01

02

33

13

01

02

41

11

52

11

43

72

62

91

1

26

54

54

64

96

55

34

84

76

84

86

65

06

35

54

74

96

24

76

56

47

07

05

96

74

74

35

14

76

66

5

36

24

94

95

16

24

34

95

36

65

96

55

16

05

85

34

25

95

27

26

43

56

35

56

15

04

95

34

06

35

4

46

86

74

16

96

45

05

05

77

36

86

96

16

56

54

84

67

36

07

67

65

26

57

07

24

15

55

84

47

46

0

56

02

32

63

35

01

42

62

55

4T

14

46

43

51

31

35

35

12

39

24

24

38

T3

01

71

93

02

44

53

31

91

8_

__

___

___

__

___

___

_T

T

ailin

g

Sa

mp

le N

o

1

vita

min

B 1 2

vi

tam

in B

2 3

vita

min

B6

4

folic

aci

d

5 v

itam

in B 1

2A

1 c

hlo

rofo

rm-n

-bu

tan

ol-

ace

tic a

cid

-am

mo

nia

(4

75

1

vv)

A

2 c

hlo

rofo

rm-n

-bu

tan

ol-

wa

ter-

ace

tic a

cid

-am

mo

nia

(3

50

55

05

v

v)

A3

be

nze

ne

-B

utyl

acet

ate-

n-pr

opan

ol-a

cetic

aci

d-am

mon

ia (

14

15

1 v

v)

A

4 c

arb

on

te

tra

chlo

ride

-bu

tyla

ceta

te-p

rop

ion

ic a

cid

-am

mo

nia

(3

79

3

vv)

A

5 c

arb

on

te

tra

chlo

rid

e-b

uty

lace

tate

-me

tha

no

l-a

mm

on

ia (

15

45

70

5

vv)

A

6 c

arb

on

te

tra

chlo

rid

e-b

uty

lace

tate

-pro

pio

nic

aci

d-m

eth

an

ol-

wa

ter

(23

10

53

v

v)

Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS


Tab

le 8

HR

f Val

ues

of V

itam

ins

on P

late

s Im

preg

nate

d w

ith D

iffer

ent C

once

ntra

tions

of H

gSO 4

A1

()

A2

()

A3

()

A4

()

A5

()

A6

()

Sam

ple

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

No

04

04

04

04

04

04

14

01

62

12

43

81

51

71

94

03

24

03

95

03

63

43

01

01

21

51

71

01

51

01

11

91

73

73

01

91

1

26

52

54

24

66

53

14

04

46

85

25

54

96

35

24

84

86

25

04

56

17

07

15

76

04

44

15

04

35

46

1

36

23

74

75

06

23

54

95

56

65

06

95

26

05

65

54

16

96

06

56

03

54

74

95

05

05

25

13

86

14

7

46

84

54

04

56

44

15

35

27

35

66

65

96

95

95

15

27

36

46

97

15

23

66

66

74

15

05

75

07

34

9

56

02

22

43

15

02

73

6T4

05

4T4

24

9T4

3

51

39

37

34

12

19

10

20

38

T2

01

012

29

38

43

33

21

16

__

__

___

___

___

__

___

S

light

tai

ling

T

T

ailin

g

Sa

mp

le N

o

1

vita

min

B 1 2

vita

min

B2

3

vita

min

B6

4

folic

aci

d

5 v

itam

in B 1

2A

1 c

hlo

rofo

rm-n

-bu

tan

ol-

ace

tic a

cid

-am

mo

nia

(4

75

1

vv)

A

2 c

hlo

rofo

rm-n

-bu

tan

ol-

wa

ter-

ace

tic a

cid

-am

mo

nia

(3

50

55

05

v

v)

A3

be

nze

ne

-B

utyl

acet

ate-

n-pr

opan

ol-a

cetic

aci

d-am

mon

ia (

14

15

1 v

v)

A

4 c

arb

on

te

tra

chlo

ride

-bu

tyla

ceta

te-p

rop

ion

ic a

cid

-am

mo

nia

(3

79

3

vv)

A

5 c

arb

on

te

tra

chlo

rid

e-b

uty

lace

tate

-me

tha

no

l-a

mm

on

ia (

15

45

70

5

vv)

A

6 c

arb

on

te

tra

chlo

rid

e-b

uty

lace

tate

-pro

pio

nic

aci

d-m

eth

an

ol-

wa

ter

(23

10

53

v

v)

Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS











Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS












Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS









Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS









Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS






ACKNOWLEDGMENTS


REFERENCES


Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS


















Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

Order now








Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS


Tab

le 3

HR

f Val

ues

of V

itam

ins

on P

late

s Im

preg

nate

d w

ith D

iffer

ent C

once

ntra

tions

of C

oSO 4

A1

()

A2

()

A3

()

A4

()

A5

()

A6

()

Sam

ple

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

No

04

04

04

04

04

04

14

03

82

02

43

82

42

52

64

04

62

24

35

04

23

53

31

01

22

01

91

01

81

51

82

22

54

24

21

81

6

26

56

45

55

96

56

06

16

16

87

34

95

16

35

53

93

66

25

96

26

17

07

16

87

05

75

85

05

46

06

8

36

26

15

75

86

25

65

65

86

67

35

15

56

05

95

85

06

96

36

96

03

56

16

56

65

55

35

65

05

96

0

46

86

56

56

76

46

86

96

17

37

67

26

06

56

46

46

37

36

77

17

35

26

77

27

46

54

86

16

47

56

5

56

05

43

44

55

03

54

0T4

15

4T5

64

2T4

95

14

74

04

01

23

02

3T2

43

8T3

93

43

64

34

04

64

92

63

5_

__

___

___

__

___

___

_T

T

ailin

g

Sa

mp

le N

o

1

vita

min

B 1 2

vi

tam

in B

2 3

vita

min

B6

4

folic

aci

d 5

vi

tam

in B 1

2A

1 c

hlo

rofo

rm-n

-bu

tan

ol-

ace

tic a

cid

-am

mo

nia

(4

75

1

vv)

A

2 c

hlo

rofo

rm-n

-bu

tan

ol-

wa

ter-

ace

tic a

cid

-am

mo

nia

(3

50

55

05

v

v)

A3

be

nze

ne

-B

utyl

acet

ate-

n-pr

opan

ol-a

cetic

aci

d-am

mon

ia (

14

15

1 v

v)

A

4 c

arb

on

te

tra

chlo

rid

e-b

uty

lace

tate

-pro

pio

nic

aci

d-a

mm

on

ia (

37

93

v

v)

A5

ca

rbo

n

tetr

ach

lori

de

-bu

tyla

ceta

te-m

eth

an

ol-

am

mo

nia

(1

54

57

05

v

v)

A6

ca

rbo

n t

etr

ach

lori

de

-bu

tyla

ceta

te-p

rop

ioni

c a

cid

-met

ha

no

l-w

ate

r (2

31

05

3

vv)

Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS


Tab

le 4

HR

f Val

ues

of V

itam

ins

on P

late

s Im

preg

nate

d w

ith D

iffer

ent C

once

ntra

tions

of N

iSO 4

A1

()

A2

()

A3

()

A4

()

A5

()

A6

()

Sam

ple

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

No

04

04

04

04

04

04

14

02

22

83

03

80

62

42

54

03

13

83

65

04

03

23

11

01

52

82

41

02

31

51

92

72

44

22

82

61

6

26

54

75

55

86

56

35

55

76

83

46

46

06

35

23

84

66

25

26

06

17

06

96

56

65

65

65

94

26

36

5

36

25

25

25

56

25

25

65

56

61

56

56

06

05

95

04

96

96

56

76

53

56

36

66

85

45

36

34

76

76

0

46

86

55

36

26

45

95

85

97

33

06

46

36

56

45

15

57

37

27

37

35

27

57

37

66

04

76

65

27

46

1_

__

___

___

__

___

___

_T

T

ailin

g

Sa

mp

le N

o

1

vita

min

B 1 2

vi

tam

in B

2 3

vita

min

B6

4

folic

aci

d

5 v

itam

in B 1

2A

1 c

hlo

rofo

rm-n

-bu

tan

ol-

ace

tic a

cid

-am

mo

nia

(4

75

1

vv)

A

2 c

hlo

rofo

rm-n

-bu

tan

ol-

wa

ter-

ace

tic a

cid

-am

mo

nia

(3

50

55

05

v

v)

A3

be

nze

ne

-B

utyl

acet

ate-

n-pr

opan

ol-a

cetic

aci

d-am

mon

ia (

14

15

1 v

v)

A

4 c

arb

on

te

tra

chlo

ride

-bu

tyla

ceta

te-p

rop

ion

ic a

cid

-am

mo

nia

(3

79

3

vv)

A

5 c

arb

on

T

etr

ach

lori

de

-bu

tyla

ceta

te-m

eth

an

ol-

am

mo

nia

(1

54

57

05

v

v)

A6

ca

rbo

n t

etr

ach

lorid

e-b

uty

lace

tate

-pro

pio

nic

aci

d-m

eth

an

ol-

wa

ter

(23

10

53

vv

)

Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS


Tab

le 5

HR

f Val

ues

of V

itam

ins

on P

late

s Im

preg

nate

d w

ith D

iffer

ent C

once

ntra

tions

of C

uSO 4

A1

()

A2

()

A3

()

A4

()

A5

()

A6

()

Sam

ple

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

No

04

04

04

04

04

04

14

03

13

94

13

82

2T2

03

14

03

54

04

45

05

24

23

51

02

02

92

71

02

41

82

13

92

94

13

63

01

9

26

56

46

56

66

56

36

06

16

86

06

66

76

35

95

65

36

26

46

36

47

07

57

07

36

45

95

75

56

47

1

36

26

06

97

56

26

05

95

86

66

16

87

06

06

36

15

06

97

47

57

63

57

26

66

77

15

56

05

96

76

8

46

86

85

76

16

46

46

36

47

37

16

47

16

56

95

24

67

37

88

07

65

27

97

98

26

05

86

36

27

57

7

56

04

24

04

95

03

93

83

55

4T

43

45

47

51

48

36

38

12

24

26

26

38

T3

32

52

74

63

44

64

02

52

5_

__

___

___

__

___

___

_T

T

ailin

g

Sa

mp

le N

o

1

vita

min

B 1 2

vi

tam

in B

2 3

vita

min

B6

4

folic

aci

d

5 v

itam

in B 1

2A

1 c

hlo

rofo

rm-n

-bu

tan

ol-

ace

tic a

cid

-am

mo

nia

(4

75

1

vv)

A

2 c

hlo

rofo

rm-n

-bu

tan

ol-

wa

ter-

ace

tic a

cid

-am

mo

nia

(3

50

55

05

v

v)

A3

be

nze

ne

-B

utyl

acet

ate-

n-pr

opan

ol-a

cetic

aci

d-am

mon

ia (

14

15

1 v

v)

A

4 c

arb

on

te

tra

chlo

ride

-bu

tyla

ceta

te-p

rop

ion

ic a

cid

-am

mo

nia

(3

79

3

vv)

A

5 c

arb

on

T

etr

ach

lori

de

-bu

tyla

ceta

te-m

eth

an

ol-

am

mo

nia

(1

54

57

05

v

v)

A6

ca

rbo

n t

etr

ach

lorid

e-b

uty

lace

tate

-pro

pio

nic

aci

d-m

eth

an

ol-

wa

ter

(23

10

53

vv

)

Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS


Tab

le 6

HR

f Val

ues

of V

itam

ins

on P

late

s Im

preg

nate

d w

ith D

iffer

ent C

once

ntra

tions

of Z

nSO 4

A1

()

A2

()

A3

()

A4

()

A5

()

A6

()

Sam

ple

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

No

04

04

04

04

04

04

14

03

93

84

03

81

8T2

12

54

02

24

24

15

03

03

53

21

01

82

72

61

03

41

21

43

62

33

92

62

71

0

26

56

06

16

36

56

54

95

36

84

06

76

16

34

44

85

16

25

86

16

27

07

85

56

16

25

05

74

46

36

9

36

25

76

67

26

25

55

76

46

64

36

85

86

04

85

54

76

96

47

06

53

55

66

06

76

96

35

93

15

86

4

46

86

65

35

56

46

06

06

17

36

36

56

56

55

45

55

47

36

67

17

35

26

07

17

95

25

86

05

27

05

8

56

04

73

24

35

03

23

32

95

4T

37

48

46

51

34

39

36

12

22

24

25

38

T4

22

12

24

12

74

53

42

31

8_

__

___

___

__

___

___

_T

T

ailin

g

Sa

mp

le N

o

1

vita

min

B 1 2

vi

tam

in B

2 3

vita

min

B6

4

folic

aci

d

5 v

itam

in B 1

2A

1 c

hlo

rofo

rm-n

-bu

tan

ol-

ace

tic a

cid

-am

mo

nia

(4

75

1

vv)

A

2 c

hlo

rofo

rm-n

-bu

tan

ol-

wa

ter-

ace

tic a

cid

-am

mo

nia

(3

50

55

05

v

v)

A3

be

nze

ne

-B

utyl

acet

ate-

n-pr

opan

ol-a

cetic

aci

d-am

mon

ia (

14

15

1 v

v)

A

4 c

arb

on

te

tra

chlo

ride

-bu

tyla

ceta

te-p

rop

ion

ic a

cid

-am

mo

nia

(3

79

3

vv)

A

5 c

arb

on

te

tra

chlo

rid

e-b

uty

lace

tate

-me

tha

no

l-a

mm

on

ia (

15

45

70

5

vv)

A

6 c

arb

on

te

tra

chlo

rid

e-b

uty

lace

tate

-pro

pio

nic

aci

d-m

eth

an

ol-

wa

ter

(23

10

53

v

v)

Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS


Tab

le 7

HR

f Val

ues

of V

itam

ins

on P

late

s Im

preg

nate

d w

ith D

iffer

ent C

once

ntra

tions

of C

dSO 4

A1

()

A2

()

A3

()

A4

()

A5

()

A6

()

Sam

ple

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

No

04

04

04

04

04

04

14

01

72

12

53

81

11

31

54

01

74

24

15

03

63

63

01

02

33

13

01

02

41

11

52

11

43

72

62

91

1

26

54

54

64

96

55

34

84

76

84

86

65

06

35

54

74

96

24

76

56

47

07

05

96

74

74

35

14

76

66

5

36

24

94

95

16

24

34

95

36

65

96

55

16

05

85

34

25

95

27

26

43

56

35

56

15

04

95

34

06

35

4

46

86

74

16

96

45

05

05

77

36

86

96

16

56

54

84

67

36

07

67

65

26

57

07

24

15

55

84

47

46

0

56

02

32

63

35

01

42

62

55

4T

14

46

43

51

31

35

35

12

39

24

24

38

T3

01

71

93

02

44

53

31

91

8_

__

___

___

__

___

___

_T

T

ailin

g

Sa

mp

le N

o

1

vita

min

B 1 2

vi

tam

in B

2 3

vita

min

B6

4

folic

aci

d

5 v

itam

in B 1

2A

1 c

hlo

rofo

rm-n

-bu

tan

ol-

ace

tic a

cid

-am

mo

nia

(4

75

1

vv)

A

2 c

hlo

rofo

rm-n

-bu

tan

ol-

wa

ter-

ace

tic a

cid

-am

mo

nia

(3

50

55

05

v

v)

A3

be

nze

ne

-B

utyl

acet

ate-

n-pr

opan

ol-a

cetic

aci

d-am

mon

ia (

14

15

1 v

v)

A

4 c

arb

on

te

tra

chlo

ride

-bu

tyla

ceta

te-p

rop

ion

ic a

cid

-am

mo

nia

(3

79

3

vv)

A

5 c

arb

on

te

tra

chlo

rid

e-b

uty

lace

tate

-me

tha

no

l-a

mm

on

ia (

15

45

70

5

vv)

A

6 c

arb

on

te

tra

chlo

rid

e-b

uty

lace

tate

-pro

pio

nic

aci

d-m

eth

an

ol-

wa

ter

(23

10

53

v

v)

Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS


Tab

le 8

HR

f Val

ues

of V

itam

ins

on P

late

s Im

preg

nate

d w

ith D

iffer

ent C

once

ntra

tions

of H

gSO 4

A1

()

A2

()

A3

()

A4

()

A5

()

A6

()

Sam

ple

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

No

04

04

04

04

04

04

14

01

62

12

43

81

51

71

94

03

24

03

95

03

63

43

01

01

21

51

71

01

51

01

11

91

73

73

01

91

1

26

52

54

24

66

53

14

04

46

85

25

54

96

35

24

84

86

25

04

56

17

07

15

76

04

44

15

04

35

46

1

36

23

74

75

06

23

54

95

56

65

06

95

26

05

65

54

16

96

06

56

03

54

74

95

05

05

25

13

86

14

7

46

84

54

04

56

44

15

35

27

35

66

65

96

95

95

15

27

36

46

97

15

23

66

66

74

15

05

75

07

34

9

56

02

22

43

15

02

73

6T4

05

4T4

24

9T4

3

51

39

37

34

12

19

10

20

38

T2

01

012

29

38

43

33

21

16

__

__

___

___

___

__

___

S

light

tai

ling

T

T

ailin

g

Sa

mp

le N

o

1

vita

min

B 1 2

vita

min

B2

3

vita

min

B6

4

folic

aci

d

5 v

itam

in B 1

2A

1 c

hlo

rofo

rm-n

-bu

tan

ol-

ace

tic a

cid

-am

mo

nia

(4

75

1

vv)

A

2 c

hlo

rofo

rm-n

-bu

tan

ol-

wa

ter-

ace

tic a

cid

-am

mo

nia

(3

50

55

05

v

v)

A3

be

nze

ne

-B

utyl

acet

ate-

n-pr

opan

ol-a

cetic

aci

d-am

mon

ia (

14

15

1 v

v)

A

4 c

arb

on

te

tra

chlo

ride

-bu

tyla

ceta

te-p

rop

ion

ic a

cid

-am

mo

nia

(3

79

3

vv)

A

5 c

arb

on

te

tra

chlo

rid

e-b

uty

lace

tate

-me

tha

no

l-a

mm

on

ia (

15

45

70

5

vv)

A

6 c

arb

on

te

tra

chlo

rid

e-b

uty

lace

tate

-pro

pio

nic

aci

d-m

eth

an

ol-

wa

ter

(23

10

53

v

v)

Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS











Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS












Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS









Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS









Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS






ACKNOWLEDGMENTS


REFERENCES


Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS


















Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

Order now








Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS


Tab

le 4

HR

f Val

ues

of V

itam

ins

on P

late

s Im

preg

nate

d w

ith D

iffer

ent C

once

ntra

tions

of N

iSO 4

A1

()

A2

()

A3

()

A4

()

A5

()

A6

()

Sam

ple

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

No

04

04

04

04

04

04

14

02

22

83

03

80

62

42

54

03

13

83

65

04

03

23

11

01

52

82

41

02

31

51

92

72

44

22

82

61

6

26

54

75

55

86

56

35

55

76

83

46

46

06

35

23

84

66

25

26

06

17

06

96

56

65

65

65

94

26

36

5

36

25

25

25

56

25

25

65

56

61

56

56

06

05

95

04

96

96

56

76

53

56

36

66

85

45

36

34

76

76

0

46

86

55

36

26

45

95

85

97

33

06

46

36

56

45

15

57

37

27

37

35

27

57

37

66

04

76

65

27

46

1_

__

___

___

__

___

___

_T

T

ailin

g

Sa

mp

le N

o

1

vita

min

B 1 2

vi

tam

in B

2 3

vita

min

B6

4

folic

aci

d

5 v

itam

in B 1

2A

1 c

hlo

rofo

rm-n

-bu

tan

ol-

ace

tic a

cid

-am

mo

nia

(4

75

1

vv)

A

2 c

hlo

rofo

rm-n

-bu

tan

ol-

wa

ter-

ace

tic a

cid

-am

mo

nia

(3

50

55

05

v

v)

A3

be

nze

ne

-B

utyl

acet

ate-

n-pr

opan

ol-a

cetic

aci

d-am

mon

ia (

14

15

1 v

v)

A

4 c

arb

on

te

tra

chlo

ride

-bu

tyla

ceta

te-p

rop

ion

ic a

cid

-am

mo

nia

(3

79

3

vv)

A

5 c

arb

on

T

etr

ach

lori

de

-bu

tyla

ceta

te-m

eth

an

ol-

am

mo

nia

(1

54

57

05

v

v)

A6

ca

rbo

n t

etr

ach

lorid

e-b

uty

lace

tate

-pro

pio

nic

aci

d-m

eth

an

ol-

wa

ter

(23

10

53

vv

)

Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS


Tab

le 5

HR

f Val

ues

of V

itam

ins

on P

late

s Im

preg

nate

d w

ith D

iffer

ent C

once

ntra

tions

of C

uSO 4

A1

()

A2

()

A3

()

A4

()

A5

()

A6

()

Sam

ple

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

No

04

04

04

04

04

04

14

03

13

94

13

82

2T2

03

14

03

54

04

45

05

24

23

51

02

02

92

71

02

41

82

13

92

94

13

63

01

9

26

56

46

56

66

56

36

06

16

86

06

66

76

35

95

65

36

26

46

36

47

07

57

07

36

45

95

75

56

47

1

36

26

06

97

56

26

05

95

86

66

16

87

06

06

36

15

06

97

47

57

63

57

26

66

77

15

56

05

96

76

8

46

86

85

76

16

46

46

36

47

37

16

47

16

56

95

24

67

37

88

07

65

27

97

98

26

05

86

36

27

57

7

56

04

24

04

95

03

93

83

55

4T

43

45

47

51

48

36

38

12

24

26

26

38

T3

32

52

74

63

44

64

02

52

5_

__

___

___

__

___

___

_T

T

ailin

g

Sa

mp

le N

o

1

vita

min

B 1 2

vi

tam

in B

2 3

vita

min

B6

4

folic

aci

d

5 v

itam

in B 1

2A

1 c

hlo

rofo

rm-n

-bu

tan

ol-

ace

tic a

cid

-am

mo

nia

(4

75

1

vv)

A

2 c

hlo

rofo

rm-n

-bu

tan

ol-

wa

ter-

ace

tic a

cid

-am

mo

nia

(3

50

55

05

v

v)

A3

be

nze

ne

-B

utyl

acet

ate-

n-pr

opan

ol-a

cetic

aci

d-am

mon

ia (

14

15

1 v

v)

A

4 c

arb

on

te

tra

chlo

ride

-bu

tyla

ceta

te-p

rop

ion

ic a

cid

-am

mo

nia

(3

79

3

vv)

A

5 c

arb

on

T

etr

ach

lori

de

-bu

tyla

ceta

te-m

eth

an

ol-

am

mo

nia

(1

54

57

05

v

v)

A6

ca

rbo

n t

etr

ach

lorid

e-b

uty

lace

tate

-pro

pio

nic

aci

d-m

eth

an

ol-

wa

ter

(23

10

53

vv

)

Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS


Tab

le 6

HR

f Val

ues

of V

itam

ins

on P

late

s Im

preg

nate

d w

ith D

iffer

ent C

once

ntra

tions

of Z

nSO 4

A1

()

A2

()

A3

()

A4

()

A5

()

A6

()

Sam

ple

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

No

04

04

04

04

04

04

14

03

93

84

03

81

8T2

12

54

02

24

24

15

03

03

53

21

01

82

72

61

03

41

21

43

62

33

92

62

71

0

26

56

06

16

36

56

54

95

36

84

06

76

16

34

44

85

16

25

86

16

27

07

85

56

16

25

05

74

46

36

9

36

25

76

67

26

25

55

76

46

64

36

85

86

04

85

54

76

96

47

06

53

55

66

06

76

96

35

93

15

86

4

46

86

65

35

56

46

06

06

17

36

36

56

56

55

45

55

47

36

67

17

35

26

07

17

95

25

86

05

27

05

8

56

04

73

24

35

03

23

32

95

4T

37

48

46

51

34

39

36

12

22

24

25

38

T4

22

12

24

12

74

53

42

31

8_

__

___

___

__

___

___

_T

T

ailin

g

Sa

mp

le N

o

1

vita

min

B 1 2

vi

tam

in B

2 3

vita

min

B6

4

folic

aci

d

5 v

itam

in B 1

2A

1 c

hlo

rofo

rm-n

-bu

tan

ol-

ace

tic a

cid

-am

mo

nia

(4

75

1

vv)

A

2 c

hlo

rofo

rm-n

-bu

tan

ol-

wa

ter-

ace

tic a

cid

-am

mo

nia

(3

50

55

05

v

v)

A3

be

nze

ne

-B

utyl

acet

ate-

n-pr

opan

ol-a

cetic

aci

d-am

mon

ia (

14

15

1 v

v)

A

4 c

arb

on

te

tra

chlo

ride

-bu

tyla

ceta

te-p

rop

ion

ic a

cid

-am

mo

nia

(3

79

3

vv)

A

5 c

arb

on

te

tra

chlo

rid

e-b

uty

lace

tate

-me

tha

no

l-a

mm

on

ia (

15

45

70

5

vv)

A

6 c

arb

on

te

tra

chlo

rid

e-b

uty

lace

tate

-pro

pio

nic

aci

d-m

eth

an

ol-

wa

ter

(23

10

53

v

v)

Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS


Tab

le 7

HR

f Val

ues

of V

itam

ins

on P

late

s Im

preg

nate

d w

ith D

iffer

ent C

once

ntra

tions

of C

dSO 4

A1

()

A2

()

A3

()

A4

()

A5

()

A6

()

Sam

ple

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

No

04

04

04

04

04

04

14

01

72

12

53

81

11

31

54

01

74

24

15

03

63

63

01

02

33

13

01

02

41

11

52

11

43

72

62

91

1

26

54

54

64

96

55

34

84

76

84

86

65

06

35

54

74

96

24

76

56

47

07

05

96

74

74

35

14

76

66

5

36

24

94

95

16

24

34

95

36

65

96

55

16

05

85

34

25

95

27

26

43

56

35

56

15

04

95

34

06

35

4

46

86

74

16

96

45

05

05

77

36

86

96

16

56

54

84

67

36

07

67

65

26

57

07

24

15

55

84

47

46

0

56

02

32

63

35

01

42

62

55

4T

14

46

43

51

31

35

35

12

39

24

24

38

T3

01

71

93

02

44

53

31

91

8_

__

___

___

__

___

___

_T

T

ailin

g

Sa

mp

le N

o

1

vita

min

B 1 2

vi

tam

in B

2 3

vita

min

B6

4

folic

aci

d

5 v

itam

in B 1

2A

1 c

hlo

rofo

rm-n

-bu

tan

ol-

ace

tic a

cid

-am

mo

nia

(4

75

1

vv)

A

2 c

hlo

rofo

rm-n

-bu

tan

ol-

wa

ter-

ace

tic a

cid

-am

mo

nia

(3

50

55

05

v

v)

A3

be

nze

ne

-B

utyl

acet

ate-

n-pr

opan

ol-a

cetic

aci

d-am

mon

ia (

14

15

1 v

v)

A

4 c

arb

on

te

tra

chlo

ride

-bu

tyla

ceta

te-p

rop

ion

ic a

cid

-am

mo

nia

(3

79

3

vv)

A

5 c

arb

on

te

tra

chlo

rid

e-b

uty

lace

tate

-me

tha

no

l-a

mm

on

ia (

15

45

70

5

vv)

A

6 c

arb

on

te

tra

chlo

rid

e-b

uty

lace

tate

-pro

pio

nic

aci

d-m

eth

an

ol-

wa

ter

(23

10

53

v

v)

Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS


Tab

le 8

HR

f Val

ues

of V

itam

ins

on P

late

s Im

preg

nate

d w

ith D

iffer

ent C

once

ntra

tions

of H

gSO 4

A1

()

A2

()

A3

()

A4

()

A5

()

A6

()

Sam

ple

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

No

04

04

04

04

04

04

14

01

62

12

43

81

51

71

94

03

24

03

95

03

63

43

01

01

21

51

71

01

51

01

11

91

73

73

01

91

1

26

52

54

24

66

53

14

04

46

85

25

54

96

35

24

84

86

25

04

56

17

07

15

76

04

44

15

04

35

46

1

36

23

74

75

06

23

54

95

56

65

06

95

26

05

65

54

16

96

06

56

03

54

74

95

05

05

25

13

86

14

7

46

84

54

04

56

44

15

35

27

35

66

65

96

95

95

15

27

36

46

97

15

23

66

66

74

15

05

75

07

34

9

56

02

22

43

15

02

73

6T4

05

4T4

24

9T4

3

51

39

37

34

12

19

10

20

38

T2

01

012

29

38

43

33

21

16

__

__

___

___

___

__

___

S

light

tai

ling

T

T

ailin

g

Sa

mp

le N

o

1

vita

min

B 1 2

vita

min

B2

3

vita

min

B6

4

folic

aci

d

5 v

itam

in B 1

2A

1 c

hlo

rofo

rm-n

-bu

tan

ol-

ace

tic a

cid

-am

mo

nia

(4

75

1

vv)

A

2 c

hlo

rofo

rm-n

-bu

tan

ol-

wa

ter-

ace

tic a

cid

-am

mo

nia

(3

50

55

05

v

v)

A3

be

nze

ne

-B

utyl

acet

ate-

n-pr

opan

ol-a

cetic

aci

d-am

mon

ia (

14

15

1 v

v)

A

4 c

arb

on

te

tra

chlo

ride

-bu

tyla

ceta

te-p

rop

ion

ic a

cid

-am

mo

nia

(3

79

3

vv)

A

5 c

arb

on

te

tra

chlo

rid

e-b

uty

lace

tate

-me

tha

no

l-a

mm

on

ia (

15

45

70

5

vv)

A

6 c

arb

on

te

tra

chlo

rid

e-b

uty

lace

tate

-pro

pio

nic

aci

d-m

eth

an

ol-

wa

ter

(23

10

53

v

v)

Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS











Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS












Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS









Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS









Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS






ACKNOWLEDGMENTS


REFERENCES


Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS


















Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

Order now








Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS


Tab

le 5

HR

f Val

ues

of V

itam

ins

on P

late

s Im

preg

nate

d w

ith D

iffer

ent C

once

ntra

tions

of C

uSO 4

A1

()

A2

()

A3

()

A4

()

A5

()

A6

()

Sam

ple

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

No

04

04

04

04

04

04

14

03

13

94

13

82

2T2

03

14

03

54

04

45

05

24

23

51

02

02

92

71

02

41

82

13

92

94

13

63

01

9

26

56

46

56

66

56

36

06

16

86

06

66

76

35

95

65

36

26

46

36

47

07

57

07

36

45

95

75

56

47

1

36

26

06

97

56

26

05

95

86

66

16

87

06

06

36

15

06

97

47

57

63

57

26

66

77

15

56

05

96

76

8

46

86

85

76

16

46

46

36

47

37

16

47

16

56

95

24

67

37

88

07

65

27

97

98

26

05

86

36

27

57

7

56

04

24

04

95

03

93

83

55

4T

43

45

47

51

48

36

38

12

24

26

26

38

T3

32

52

74

63

44

64

02

52

5_

__

___

___

__

___

___

_T

T

ailin

g

Sa

mp

le N

o

1

vita

min

B 1 2

vi

tam

in B

2 3

vita

min

B6

4

folic

aci

d

5 v

itam

in B 1

2A

1 c

hlo

rofo

rm-n

-bu

tan

ol-

ace

tic a

cid

-am

mo

nia

(4

75

1

vv)

A

2 c

hlo

rofo

rm-n

-bu

tan

ol-

wa

ter-

ace

tic a

cid

-am

mo

nia

(3

50

55

05

v

v)

A3

be

nze

ne

-B

utyl

acet

ate-

n-pr

opan

ol-a

cetic

aci

d-am

mon

ia (

14

15

1 v

v)

A

4 c

arb

on

te

tra

chlo

ride

-bu

tyla

ceta

te-p

rop

ion

ic a

cid

-am

mo

nia

(3

79

3

vv)

A

5 c

arb

on

T

etr

ach

lori

de

-bu

tyla

ceta

te-m

eth

an

ol-

am

mo

nia

(1

54

57

05

v

v)

A6

ca

rbo

n t

etr

ach

lorid

e-b

uty

lace

tate

-pro

pio

nic

aci

d-m

eth

an

ol-

wa

ter

(23

10

53

vv

)

Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS


Tab

le 6

HR

f Val

ues

of V

itam

ins

on P

late

s Im

preg

nate

d w

ith D

iffer

ent C

once

ntra

tions

of Z

nSO 4

A1

()

A2

()

A3

()

A4

()

A5

()

A6

()

Sam

ple

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

No

04

04

04

04

04

04

14

03

93

84

03

81

8T2

12

54

02

24

24

15

03

03

53

21

01

82

72

61

03

41

21

43

62

33

92

62

71

0

26

56

06

16

36

56

54

95

36

84

06

76

16

34

44

85

16

25

86

16

27

07

85

56

16

25

05

74

46

36

9

36

25

76

67

26

25

55

76

46

64

36

85

86

04

85

54

76

96

47

06

53

55

66

06

76

96

35

93

15

86

4

46

86

65

35

56

46

06

06

17

36

36

56

56

55

45

55

47

36

67

17

35

26

07

17

95

25

86

05

27

05

8

56

04

73

24

35

03

23

32

95

4T

37

48

46

51

34

39

36

12

22

24

25

38

T4

22

12

24

12

74

53

42

31

8_

__

___

___

__

___

___

_T

T

ailin

g

Sa

mp

le N

o

1

vita

min

B 1 2

vi

tam

in B

2 3

vita

min

B6

4

folic

aci

d

5 v

itam

in B 1

2A

1 c

hlo

rofo

rm-n

-bu

tan

ol-

ace

tic a

cid

-am

mo

nia

(4

75

1

vv)

A

2 c

hlo

rofo

rm-n

-bu

tan

ol-

wa

ter-

ace

tic a

cid

-am

mo

nia

(3

50

55

05

v

v)

A3

be

nze

ne

-B

utyl

acet

ate-

n-pr

opan

ol-a

cetic

aci

d-am

mon

ia (

14

15

1 v

v)

A

4 c

arb

on

te

tra

chlo

ride

-bu

tyla

ceta

te-p

rop

ion

ic a

cid

-am

mo

nia

(3

79

3

vv)

A

5 c

arb

on

te

tra

chlo

rid

e-b

uty

lace

tate

-me

tha

no

l-a

mm

on

ia (

15

45

70

5

vv)

A

6 c

arb

on

te

tra

chlo

rid

e-b

uty

lace

tate

-pro

pio

nic

aci

d-m

eth

an

ol-

wa

ter

(23

10

53

v

v)

Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS


Tab

le 7

HR

f Val

ues

of V

itam

ins

on P

late

s Im

preg

nate

d w

ith D

iffer

ent C

once

ntra

tions

of C

dSO 4

A1

()

A2

()

A3

()

A4

()

A5

()

A6

()

Sam

ple

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

No

04

04

04

04

04

04

14

01

72

12

53

81

11

31

54

01

74

24

15

03

63

63

01

02

33

13

01

02

41

11

52

11

43

72

62

91

1

26

54

54

64

96

55

34

84

76

84

86

65

06

35

54

74

96

24

76

56

47

07

05

96

74

74

35

14

76

66

5

36

24

94

95

16

24

34

95

36

65

96

55

16

05

85

34

25

95

27

26

43

56

35

56

15

04

95

34

06

35

4

46

86

74

16

96

45

05

05

77

36

86

96

16

56

54

84

67

36

07

67

65

26

57

07

24

15

55

84

47

46

0

56

02

32

63

35

01

42

62

55

4T

14

46

43

51

31

35

35

12

39

24

24

38

T3

01

71

93

02

44

53

31

91

8_

__

___

___

__

___

___

_T

T

ailin

g

Sa

mp

le N

o

1

vita

min

B 1 2

vi

tam

in B

2 3

vita

min

B6

4

folic

aci

d

5 v

itam

in B 1

2A

1 c

hlo

rofo

rm-n

-bu

tan

ol-

ace

tic a

cid

-am

mo

nia

(4

75

1

vv)

A

2 c

hlo

rofo

rm-n

-bu

tan

ol-

wa

ter-

ace

tic a

cid

-am

mo

nia

(3

50

55

05

v

v)

A3

be

nze

ne

-B

utyl

acet

ate-

n-pr

opan

ol-a

cetic

aci

d-am

mon

ia (

14

15

1 v

v)

A

4 c

arb

on

te

tra

chlo

ride

-bu

tyla

ceta

te-p

rop

ion

ic a

cid

-am

mo

nia

(3

79

3

vv)

A

5 c

arb

on

te

tra

chlo

rid

e-b

uty

lace

tate

-me

tha

no

l-a

mm

on

ia (

15

45

70

5

vv)

A

6 c

arb

on

te

tra

chlo

rid

e-b

uty

lace

tate

-pro

pio

nic

aci

d-m

eth

an

ol-

wa

ter

(23

10

53

v

v)

Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS


Tab

le 8

HR

f Val

ues

of V

itam

ins

on P

late

s Im

preg

nate

d w

ith D

iffer

ent C

once

ntra

tions

of H

gSO 4

A1

()

A2

()

A3

()

A4

()

A5

()

A6

()

Sam

ple

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

No

04

04

04

04

04

04

14

01

62

12

43

81

51

71

94

03

24

03

95

03

63

43

01

01

21

51

71

01

51

01

11

91

73

73

01

91

1

26

52

54

24

66

53

14

04

46

85

25

54

96

35

24

84

86

25

04

56

17

07

15

76

04

44

15

04

35

46

1

36

23

74

75

06

23

54

95

56

65

06

95

26

05

65

54

16

96

06

56

03

54

74

95

05

05

25

13

86

14

7

46

84

54

04

56

44

15

35

27

35

66

65

96

95

95

15

27

36

46

97

15

23

66

66

74

15

05

75

07

34

9

56

02

22

43

15

02

73

6T4

05

4T4

24

9T4

3

51

39

37

34

12

19

10

20

38

T2

01

012

29

38

43

33

21

16

__

__

___

___

___

__

___

S

light

tai

ling

T

T

ailin

g

Sa

mp

le N

o

1

vita

min

B 1 2

vita

min

B2

3

vita

min

B6

4

folic

aci

d

5 v

itam

in B 1

2A

1 c

hlo

rofo

rm-n

-bu

tan

ol-

ace

tic a

cid

-am

mo

nia

(4

75

1

vv)

A

2 c

hlo

rofo

rm-n

-bu

tan

ol-

wa

ter-

ace

tic a

cid

-am

mo

nia

(3

50

55

05

v

v)

A3

be

nze

ne

-B

utyl

acet

ate-

n-pr

opan

ol-a

cetic

aci

d-am

mon

ia (

14

15

1 v

v)

A

4 c

arb

on

te

tra

chlo

ride

-bu

tyla

ceta

te-p

rop

ion

ic a

cid

-am

mo

nia

(3

79

3

vv)

A

5 c

arb

on

te

tra

chlo

rid

e-b

uty

lace

tate

-me

tha

no

l-a

mm

on

ia (

15

45

70

5

vv)

A

6 c

arb

on

te

tra

chlo

rid

e-b

uty

lace

tate

-pro

pio

nic

aci

d-m

eth

an

ol-

wa

ter

(23

10

53

v

v)

Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS











Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS












Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS









Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS









Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS






ACKNOWLEDGMENTS


REFERENCES


Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS


















Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

Order now








Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS


Tab

le 6

HR

f Val

ues

of V

itam

ins

on P

late

s Im

preg

nate

d w

ith D

iffer

ent C

once

ntra

tions

of Z

nSO 4

A1

()

A2

()

A3

()

A4

()

A5

()

A6

()

Sam

ple

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

No

04

04

04

04

04

04

14

03

93

84

03

81

8T2

12

54

02

24

24

15

03

03

53

21

01

82

72

61

03

41

21

43

62

33

92

62

71

0

26

56

06

16

36

56

54

95

36

84

06

76

16

34

44

85

16

25

86

16

27

07

85

56

16

25

05

74

46

36

9

36

25

76

67

26

25

55

76

46

64

36

85

86

04

85

54

76

96

47

06

53

55

66

06

76

96

35

93

15

86

4

46

86

65

35

56

46

06

06

17

36

36

56

56

55

45

55

47

36

67

17

35

26

07

17

95

25

86

05

27

05

8

56

04

73

24

35

03

23

32

95

4T

37

48

46

51

34

39

36

12

22

24

25

38

T4

22

12

24

12

74

53

42

31

8_

__

___

___

__

___

___

_T

T

ailin

g

Sa

mp

le N

o

1

vita

min

B 1 2

vi

tam

in B

2 3

vita

min

B6

4

folic

aci

d

5 v

itam

in B 1

2A

1 c

hlo

rofo

rm-n

-bu

tan

ol-

ace

tic a

cid

-am

mo

nia

(4

75

1

vv)

A

2 c

hlo

rofo

rm-n

-bu

tan

ol-

wa

ter-

ace

tic a

cid

-am

mo

nia

(3

50

55

05

v

v)

A3

be

nze

ne

-B

utyl

acet

ate-

n-pr

opan

ol-a

cetic

aci

d-am

mon

ia (

14

15

1 v

v)

A

4 c

arb

on

te

tra

chlo

ride

-bu

tyla

ceta

te-p

rop

ion

ic a

cid

-am

mo

nia

(3

79

3

vv)

A

5 c

arb

on

te

tra

chlo

rid

e-b

uty

lace

tate

-me

tha

no

l-a

mm

on

ia (

15

45

70

5

vv)

A

6 c

arb

on

te

tra

chlo

rid

e-b

uty

lace

tate

-pro

pio

nic

aci

d-m

eth

an

ol-

wa

ter

(23

10

53

v

v)

Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS


Tab

le 7

HR

f Val

ues

of V

itam

ins

on P

late

s Im

preg

nate

d w

ith D

iffer

ent C

once

ntra

tions

of C

dSO 4

A1

()

A2

()

A3

()

A4

()

A5

()

A6

()

Sam

ple

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

No

04

04

04

04

04

04

14

01

72

12

53

81

11

31

54

01

74

24

15

03

63

63

01

02

33

13

01

02

41

11

52

11

43

72

62

91

1

26

54

54

64

96

55

34

84

76

84

86

65

06

35

54

74

96

24

76

56

47

07

05

96

74

74

35

14

76

66

5

36

24

94

95

16

24

34

95

36

65

96

55

16

05

85

34

25

95

27

26

43

56

35

56

15

04

95

34

06

35

4

46

86

74

16

96

45

05

05

77

36

86

96

16

56

54

84

67

36

07

67

65

26

57

07

24

15

55

84

47

46

0

56

02

32

63

35

01

42

62

55

4T

14

46

43

51

31

35

35

12

39

24

24

38

T3

01

71

93

02

44

53

31

91

8_

__

___

___

__

___

___

_T

T

ailin

g

Sa

mp

le N

o

1

vita

min

B 1 2

vi

tam

in B

2 3

vita

min

B6

4

folic

aci

d

5 v

itam

in B 1

2A

1 c

hlo

rofo

rm-n

-bu

tan

ol-

ace

tic a

cid

-am

mo

nia

(4

75

1

vv)

A

2 c

hlo

rofo

rm-n

-bu

tan

ol-

wa

ter-

ace

tic a

cid

-am

mo

nia

(3

50

55

05

v

v)

A3

be

nze

ne

-B

utyl

acet

ate-

n-pr

opan

ol-a

cetic

aci

d-am

mon

ia (

14

15

1 v

v)

A

4 c

arb

on

te

tra

chlo

ride

-bu

tyla

ceta

te-p

rop

ion

ic a

cid

-am

mo

nia

(3

79

3

vv)

A

5 c

arb

on

te

tra

chlo

rid

e-b

uty

lace

tate

-me

tha

no

l-a

mm

on

ia (

15

45

70

5

vv)

A

6 c

arb

on

te

tra

chlo

rid

e-b

uty

lace

tate

-pro

pio

nic

aci

d-m

eth

an

ol-

wa

ter

(23

10

53

v

v)

Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS


Tab

le 8

HR

f Val

ues

of V

itam

ins

on P

late

s Im

preg

nate

d w

ith D

iffer

ent C

once

ntra

tions

of H

gSO 4

A1

()

A2

()

A3

()

A4

()

A5

()

A6

()

Sam

ple

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

No

04

04

04

04

04

04

14

01

62

12

43

81

51

71

94

03

24

03

95

03

63

43

01

01

21

51

71

01

51

01

11

91

73

73

01

91

1

26

52

54

24

66

53

14

04

46

85

25

54

96

35

24

84

86

25

04

56

17

07

15

76

04

44

15

04

35

46

1

36

23

74

75

06

23

54

95

56

65

06

95

26

05

65

54

16

96

06

56

03

54

74

95

05

05

25

13

86

14

7

46

84

54

04

56

44

15

35

27

35

66

65

96

95

95

15

27

36

46

97

15

23

66

66

74

15

05

75

07

34

9

56

02

22

43

15

02

73

6T4

05

4T4

24

9T4

3

51

39

37

34

12

19

10

20

38

T2

01

012

29

38

43

33

21

16

__

__

___

___

___

__

___

S

light

tai

ling

T

T

ailin

g

Sa

mp

le N

o

1

vita

min

B 1 2

vita

min

B2

3

vita

min

B6

4

folic

aci

d

5 v

itam

in B 1

2A

1 c

hlo

rofo

rm-n

-bu

tan

ol-

ace

tic a

cid

-am

mo

nia

(4

75

1

vv)

A

2 c

hlo

rofo

rm-n

-bu

tan

ol-

wa

ter-

ace

tic a

cid

-am

mo

nia

(3

50

55

05

v

v)

A3

be

nze

ne

-B

utyl

acet

ate-

n-pr

opan

ol-a

cetic

aci

d-am

mon

ia (

14

15

1 v

v)

A

4 c

arb

on

te

tra

chlo

ride

-bu

tyla

ceta

te-p

rop

ion

ic a

cid

-am

mo

nia

(3

79

3

vv)

A

5 c

arb

on

te

tra

chlo

rid

e-b

uty

lace

tate

-me

tha

no

l-a

mm

on

ia (

15

45

70

5

vv)

A

6 c

arb

on

te

tra

chlo

rid

e-b

uty

lace

tate

-pro

pio

nic

aci

d-m

eth

an

ol-

wa

ter

(23

10

53

v

v)

Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS











Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS












Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS









Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS









Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS






ACKNOWLEDGMENTS


REFERENCES


Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS


















Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

Order now








Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS


Tab

le 7

HR

f Val

ues

of V

itam

ins

on P

late

s Im

preg

nate

d w

ith D

iffer

ent C

once

ntra

tions

of C

dSO 4

A1

()

A2

()

A3

()

A4

()

A5

()

A6

()

Sam

ple

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

No

04

04

04

04

04

04

14

01

72

12

53

81

11

31

54

01

74

24

15

03

63

63

01

02

33

13

01

02

41

11

52

11

43

72

62

91

1

26

54

54

64

96

55

34

84

76

84

86

65

06

35

54

74

96

24

76

56

47

07

05

96

74

74

35

14

76

66

5

36

24

94

95

16

24

34

95

36

65

96

55

16

05

85

34

25

95

27

26

43

56

35

56

15

04

95

34

06

35

4

46

86

74

16

96

45

05

05

77

36

86

96

16

56

54

84

67

36

07

67

65

26

57

07

24

15

55

84

47

46

0

56

02

32

63

35

01

42

62

55

4T

14

46

43

51

31

35

35

12

39

24

24

38

T3

01

71

93

02

44

53

31

91

8_

__

___

___

__

___

___

_T

T

ailin

g

Sa

mp

le N

o

1

vita

min

B 1 2

vi

tam

in B

2 3

vita

min

B6

4

folic

aci

d

5 v

itam

in B 1

2A

1 c

hlo

rofo

rm-n

-bu

tan

ol-

ace

tic a

cid

-am

mo

nia

(4

75

1

vv)

A

2 c

hlo

rofo

rm-n

-bu

tan

ol-

wa

ter-

ace

tic a

cid

-am

mo

nia

(3

50

55

05

v

v)

A3

be

nze

ne

-B

utyl

acet

ate-

n-pr

opan

ol-a

cetic

aci

d-am

mon

ia (

14

15

1 v

v)

A

4 c

arb

on

te

tra

chlo

ride

-bu

tyla

ceta

te-p

rop

ion

ic a

cid

-am

mo

nia

(3

79

3

vv)

A

5 c

arb

on

te

tra

chlo

rid

e-b

uty

lace

tate

-me

tha

no

l-a

mm

on

ia (

15

45

70

5

vv)

A

6 c

arb

on

te

tra

chlo

rid

e-b

uty

lace

tate

-pro

pio

nic

aci

d-m

eth

an

ol-

wa

ter

(23

10

53

v

v)

Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS


Tab

le 8

HR

f Val

ues

of V

itam

ins

on P

late

s Im

preg

nate

d w

ith D

iffer

ent C

once

ntra

tions

of H

gSO 4

A1

()

A2

()

A3

()

A4

()

A5

()

A6

()

Sam

ple

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

No

04

04

04

04

04

04

14

01

62

12

43

81

51

71

94

03

24

03

95

03

63

43

01

01

21

51

71

01

51

01

11

91

73

73

01

91

1

26

52

54

24

66

53

14

04

46

85

25

54

96

35

24

84

86

25

04

56

17

07

15

76

04

44

15

04

35

46

1

36

23

74

75

06

23

54

95

56

65

06

95

26

05

65

54

16

96

06

56

03

54

74

95

05

05

25

13

86

14

7

46

84

54

04

56

44

15

35

27

35

66

65

96

95

95

15

27

36

46

97

15

23

66

66

74

15

05

75

07

34

9

56

02

22

43

15

02

73

6T4

05

4T4

24

9T4

3

51

39

37

34

12

19

10

20

38

T2

01

012

29

38

43

33

21

16

__

__

___

___

___

__

___

S

light

tai

ling

T

T

ailin

g

Sa

mp

le N

o

1

vita

min

B 1 2

vita

min

B2

3

vita

min

B6

4

folic

aci

d

5 v

itam

in B 1

2A

1 c

hlo

rofo

rm-n

-bu

tan

ol-

ace

tic a

cid

-am

mo

nia

(4

75

1

vv)

A

2 c

hlo

rofo

rm-n

-bu

tan

ol-

wa

ter-

ace

tic a

cid

-am

mo

nia

(3

50

55

05

v

v)

A3

be

nze

ne

-B

utyl

acet

ate-

n-pr

opan

ol-a

cetic

aci

d-am

mon

ia (

14

15

1 v

v)

A

4 c

arb

on

te

tra

chlo

ride

-bu

tyla

ceta

te-p

rop

ion

ic a

cid

-am

mo

nia

(3

79

3

vv)

A

5 c

arb

on

te

tra

chlo

rid

e-b

uty

lace

tate

-me

tha

no

l-a

mm

on

ia (

15

45

70

5

vv)

A

6 c

arb

on

te

tra

chlo

rid

e-b

uty

lace

tate

-pro

pio

nic

aci

d-m

eth

an

ol-

wa

ter

(23

10

53

v

v)

Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS











Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS












Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS









Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS









Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS






ACKNOWLEDGMENTS


REFERENCES


Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS


















Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

Order now








Dow

nloa

ded

by [

Uni

vers

ity o

f C

alif

orni

a Sa

nta

Cru

z] a

t 16

23 0

2 N

ovem

ber

2014

ORDER REPRINTS


Tab

le 8

HR

f Val

ues

of V

itam

ins

on P

late

s Im

preg

nate

d w

ith D

iffer

ent C

once

ntra

tions

of H

gSO 4

A1

()

A2

()

A3

()

A4

()

A5

()

A6

()

Sam

ple

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

Pla

in0

10

20

3P

lain

01

02

03

No

04

04

04

04

04

04

14

01

62

12

43

81

51

71

94

03

24

03

95

03

63

43

01

01

21

51

71

01

51

01

11

91

73

73

01

91

1

26

52

54

24

66

53

14

04

46

85

25

54

96

35

24

84

86

25

04

56

17

07

15

76

04

44

15

04

35

46

1

36

23

74

75

06

23

54

95

56

65

06

95

26

05

65

54

16

96

06

56

03

54

74

95

05

05

25

13

86

14

7

46

84

54

04

56

44

15

35

27

35

66

65

96

95

95

15

27

36

46

97

15

23

66

66

74

15

05

75

07

34

9

56

02

22

43

15

02

73

6T4

05

4T4

24

9T4

3

51

39

37

34

12

19

10

20

38

T2

01

012

29

38

43

33

21

16

__

__

___

___

___

__

___

S

light

tai

ling

T

T

ailin

g

Sa

mp

le N

o

1

vita

min

B 1 2

vita

min

B2

3

vita

min

B6

4

folic

aci

d

5 v

itam

in B 1

2A

1 c

hlo

rofo

rm-n

-bu

tan

ol-

ace

tic a

cid

-am

mo

nia

(4

75

1

vv)

A

2 c

hlo

rofo

rm-n

-bu

tan

ol-

wa

ter-

ace

tic a

cid

-am

mo

nia

(3

50

55

05

v

v)

A3

be

nze

ne

-B

utyl

acet

ate-

n-pr

opan

ol-a

cetic

aci

d-am

mon

ia (

14

15

1 v

v)

A

4 c

arb

on

te

tra

chlo

ride

-bu

tyla

ceta

te-p

rop

ion

ic a

cid

-am

mo

nia

(3

79

3

vv)

A

5 c

arb

on

te

tra

chlo

rid

e-b

uty

lace

tate

-me

tha

no

l-a

mm

on

ia (

15

45

70

5

vv)

A

6 c

arb

on

te

tra

chlo

rid

e-b

uty

lace

tate

-pro

pio

nic

aci

d-m

eth

an

ol-

wa

ter

(23

10

53

v

v)

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improved separation of vitamin b complex and folic acid using some new solvent systems and...

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