In the name of GOD

Molecular imprinted polymer(MIP)

Supervisor

Dr. Parchehbaf

Presenter

Meysam abdi

1391/09/152012/12/05

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Content

Introduction

History

Principles

Advantage

Application

Conclusion

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Introduction

Base of the method, is recording of target molecule structure

In Polymer memory at polymerization process.

so that the identification, synthesized polymer only detects the

target molecule.

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History

1894, Fischer lock & key theory.

1930, Polyakov, molecular imprinting in silica’s matrix

1949, Linus pauling theory, change of antibody structures

1977, Gunter wulff, molecular imprinting by using covalence banding

1981, Klaus mosbach, molecular imprinting by using non-covalence banding

1995, Witcombe, molecular imprinting by using semi-covalence (hybrid) banding

www.wikipedia.com www.sciencedirect.com

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Research procedure

C. Alexander, L. Davidson, and W. Hayes, Tetrahedron 59, 2025-2057 (2003).

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Principles

www.biotage.com

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Fischer Lock & Key theory

Monomer & cross linker

Template molecule

bonding

Remove template molecule

PowerPoint of J.jafari thesis

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Variety methods of MIP production

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Advantage

high selectivity

high pre-Concentration factor

the sorbent is reusable for many times

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Application of MIP

MIP

Sensor

Pre-concentratio

n

Pharmacy

Catalyses

Separation

Selective sorption

Purification

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Sensor

Development of a voltammetric sensor based on a molecularly

imprinted polymer(MIP) for caffeine measurement

Step1: A caffeine-selective molecularly imprinted polymer (MIP) and a non-imprinted polymer (NIP) synthesized.

Step2: The MIP, embedded in the carbon paste electrode.

Step3:The prepared electrode was used for caffeine measurement via: 1.analyte extraction in the electrode 2.electrode washing 3.electrochemical measurement of caffeine.

Step4: DPV were used for determination.

A linear range of calibration curve: 6×10-8 to 2.5×10-5 mol/L

The detection limit: 1.5×10-8 mol/L.

T. Alizadeh et al. / Electrochimica Acta 55 (2010) 1568–1574

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Caffeine (1,3,7-trimethylxanthine) is an alkaloid

Caffeine Fountain

Tea

kola nuts

Cacao beans

Coffee

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MIP-CP & NIP-CP responses

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MIP-CP electrode selectivity

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Real sample analysis

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A novel high selective and sensitive para-nitrophenol volta-

-mmetric sensor, based on a MIP-CP electrode

Step1: Para-nitrophenol selective MIP and a non-imprinted polymer (NIP) synthesized

Step2: used for carbon paste (CP) electrode preparation.

Step3: A dynamic linear range of 8×10-9 to 5×10-6 mol /L was obtained. The detection limit of the sensor was calculated as 3×10-9 mol/L.

T. Alizadeh et al. / Talanta 79 (2009) 1197–1203

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Electrode responses & washing effect

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Selection of elecrochemical methods

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Analytical characterization

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Application of an Hg2+ selective imprinted polymer for the preparation of a

novel highly selective and sensitive electrochemical sensor for the

determination of ultra trace Hg2+

Step1: Mercury ion selective cavities were created in the vinyl pyridine based cross-linked polymer.

Step2: The interference of different ionic species with the response of the electrode was also studied.

Step3: This sensor showed a linear response range of 2.5×10-9 to 5.0×10-7 M and lower detection limit of 5.2×10−10 M (S/N).

T. Alizadeh et al. / Analytica Chimica Acta 689 (2011) 52–59

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Hg2+

Properties

Hg2+ can in turn cause symptoms

paranoia

Sleeplessness fever memory

lossweakness

tremors

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Comparison of the IIP-CP & NIP-CP electrodes

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Evaluation the selectivity of sensor

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Determination of Hg2+ in real samples

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Preparation of nano-sized Pb2+ imprinted polymer and its application as the

chemical interface of an electrochemical sensor for toxic lead determination

indifferent real samples

Step1: a new nano-structured ion imprinted polymer (IIP) was synthesized by copolymerization

Step2: A carbon paste electrode modified with IIP-nanoparticles was used for fabrication of a Pb2+ sensitive electrode.

Step3: Differential pulse stripping voltammetry method was applied as the determination technique,

Step4: The introduced sensor showed a linear range of 1.0×10-9 to 8.1×10-7 M and detection limit of 6.0×10-10 (S/N = 3).

T. Alizadeh, S. Amjadi / Journal of Hazardous Materials 190 (2011) 451–459

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Pb2+

importance

toxicological

Effects

interferes with

calciumPoisoner

environmental

pollutant

enzyme inhibitor

Found in Soil

Air

Water

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Comparison of the prepared electrodes

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Evaluation of the selectivity of IIP-CP electrode

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Evaluation of the effect of extraction conditions

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Determination of Pb2+ in real samples by proposed sensor

T. Alizadeh et al. / Talanta 79 (2009) 1197–1203

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Prepared electrode

T. Alizadeh et al. / Talanta 79 (2009) 1197–1203

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a new electrochemical sensor for determination of compounds

at trace levels was introduced. Application of polymer as a

novel modifying agent in the carbon paste electrode made it

very selective for compounds determination in the presence of

common potential interfering agents.

The polymer, used in the carbon paste composition, acted

as the selective chemical interface of the sensor as well as a

pre-concentration agent.

Conclusion

Thanks for your attention

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