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Maturation and Spawning of Hyperoglyphe japonica in the southwestern Japan Sea off Yamaguchi Prefecture

Mitsuhisa KAWANO and Yuji SHIGENAGA

Maturation process and spawning of Hyperoglyphe japonica were investigated by histological observation of the gonads, frequency distributions of the oocyte diameters and gonadosomatic index (GSI). We found that the fish spawned at this area in Japan Sea and assumed the fish to be multiple spawners, because the fish had both maturing eggs and postovulatory follicles. The spawning period was estimated to be during October-March based on the GSI. The minimum sizes of mature fish were 4 73 mm FL in male and 573 mm FL in female.

Key words : Hyperoglyphejaponica; Maturation; Spawning; Southwestern Japan Sea

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Fig. I Map showing the sampling location (shaded area) of Hyperoglyphe japonica. Samples were caught by angling or gill nets.

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Fig.2 Micrographs of ovaries in Hyperoglyphe japonica. A: immature phase, B: developing phase, C: maturation phase, D: spent phase. P: oocyte at peripheral nucleolus stage, YV: oocyte at yolk vesicle stage, YG: oocyte at yolk globule stage, M: egg at mature stage. F: postovulatory follicle . Bar=250 p,m.

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Table I Details of mature or spent females of Hyperoglyphe japonica used in the study.

Date FL(mm) GSI Maturity Qhase Jan. 20, 2009 628 5.2 Mature Feb. 5,2009 604 5.9 Mature Feb. 5,2009 605 2.2 Mature (spawning) Oct. 12, 2009 635 1.9 Mature Dec. 2,2009 573 1.5 Mature Jan.20,2009 644 0.8 Spent Jan. 20, 2009 625 0.6 Spent Feb. 26, 2009 632 0.7 Spent Mar. 17, 2010 688 1.2 Spent Mar. 17, 2010 713 1.2 Spent Mar. 6, 2011 696 1.6 Spent Mar. 23, 2011 740 1.4 SQent

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Table 2 Frequency of females by fork length.

Fork length (mm) 450- 499 500- 549 No. of femlales 8 20 No. of males 6 31 Frequency of females (%) 57.1 39.2 *p<0.05 (Binominal test)

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