Information about Sources of Protein Expression SystemProteins are large biological molecules. It consists of amino acid residues and polypeptide. These are essential parts of an organism and participate in every process within cells. Its blueprints are stored in DNA and decoded to produce messenger mRNA. This message is then translated into a protein. Translation process includes initiation, elongation and termination steps. Protein expression refers to the method during which proteins are synthesized, changed and controlled in human bodies. There are special technological tools to undertake hard-to-express and hard-to-dissolve proteins. With the correct method, the expression level for the proteins can be greatly improved. There are manufacturing company consist of biotechnologists to produce proteins products and antibiotics. The researchers apply different methods to study protein structure and its function. Some of these methods are immune-histochemistry, X-ray crystallography, site-directed mutagenesis, mass spectrometry and nuclear magnetic resonance.The sources of recombinant proteins are humans, mouse, E. coli, insect, yeast etc. and the amount of protein yield in these hosts differs significantly in different cell strains. Human cells are generally capable of producing larger amounts of secreted proteins. A further division of protein expression is:a. Prokaryotic expression systems includes Escherichia coli, Lactococcus lactis or other bacteria; b. Yeast includes Pichia pastoris, Pichia methanolica etc.c. Insect cells consist of baculovirus, Invitrogen, Drosophila. d. Mammalian cellse. Others: Transgenic mice, transgenic plants etc. One of the common technologies used for protein yield is BacTECTM system. This expression is produced in a different volume of a culture. The quantity is measured in micrograms, milligrams or grams. All the structural units determine the expression level of the recombinant protein. With technological advancement, it has become easier to protein expression and purification, provided the company have all the necessary tool arrangement and well equipped laboratory. The various techniques include- electrophoresis, ultracentrifugation, chromatography and precipitation.Contact Details:Company: Creative BioMartPhone: 1-631-559-9269 Fax: 1-631-938-8127Address: 45-16 Ramsey Road, Shirley, NY 11967, USAEmail: info@creative-biomart.comWebsite: http://www.creativebiomart.net/