information management the old fashioned way! gary r. skuse, ph.d. director of bioinformatics...
TRANSCRIPT
Information Management
The old fashioned way!
Gary R. Skuse, Ph.D.Director of Bioinformatics
Essential Cell Biology : An Introduction to the
Molecular Biology of the Cell
by Bruce Alberts, Dennis Bray, Alexander Johnson, Julian Lewis, Peter Walter, Keith Roberts, Martin Raff
Garland Pub; ISBN: 0815320450
All Illustrations taken from:
Molecular Biology is about Information
Alice
Information Flow within Cells
Spatial Flow of Information
DNA is Organized into Chromosomes
Painted Chromosomes
Electron Micrographs of Chromosomes
Chromosomes are Comprised of DNA
Packaged with Proteins
Chromosomes Contain Large Quantities of DNA
DNA can Replicate Itself
DNA Replication is Uncommonly Accurate
Information in DNA is Transcribed into RNA
There are Different Types of RNA
Gene Organization in Prokaryotes
Translation of Prokaryotic and Eukaryotic RNA
DNA Serves as a Template for Transcription
Eukaryotic Genes are Not Contiguous
RNA Splicing is Sequence Directed
Alternative Splicing Leads to Multiple Proteins from One Gene
RNA Processing, Like All Things Biological, is
Complex
Gene Expression is Regulated Differently for Different Genes
RNA is Translated into Protein
The Code Must be Parsed Accurately
Linear Strings of Amino Acids Interact
Proteins Fold to Form 3-D Structures
We can Depict Protein Structures in
Many Ways
We can Depict Protein Structures in
Many Ways
We can Depict Protein Structures in
Many Ways
We can Depict Protein Structures in
Many Ways
Protein Shape Leads to Function
Protein Shape Leads to Function
Proteins may Change Shape as they Function
Genes are Somewhat Modular
Protein Structure Reflects that Modularity
Preparation of DNA for Cloning
Cloning of DNA
The PCR