H I G H - T E C H D I S P O S A B L E S

Innovative cell culture plates and inserts from BRAND for manual and automated cell and tissue culture work!

Insert SystemBRANDplates®

n 24-well standard plate and the 6-well special plate for use with up to six strips of 4 inserts

n Side feed ports in the well for changing media with minimal disruption – without moving inserts

n Perfect for use with pipetting robots

n Especially for tissue culture: Inlet Opening System inserts with inlet channels.

n 4 pore sizes for applications such as co-culture, migration/invasion assays, 3D tissue preparation and more

Innovation in every detail, inspired design!

BRANDplates® Insert SystemThe BRANDplates® Insert System, co-developed with the Fraunhofer Society, offers an innovative expansion of the BRAND cell culture product line.

The BRAND Insert System includes two different cell culture plates and two types of cell culture inserts, which can be used in combination. The cell culture plates and the associated inserts are available in both a 24-well standard and a 6-well special plate. These can be used in a wide variety of applications. The standard model is used in such applications as co-culture, secretion studies, and chemotaxis tests, and the special model finds application in the automated in vitro preparation of human tissues (3-D tissue culture). Skin, epidermis, and corneal models, etc., have taken on greater importance due to EU or European legislation restricting animal experimentation to a minimum. These tissues are now employed on a daily basis for tolerance studies, toxicity tests, and irritation tests in the pharmaceutical and cosmetics industries.

6-well special plate Four wells are all connected as one large, elongated well. This well can be fitted with a strip of 4 inserts so that all 4 of the inserts in the strip can be supplied with medium at the same time. Particularly well suited to the use of insert strips with inlet channels.

The well and insert are perfectly coordinated:The guide grooves in the support collars of the wells in the 24-well standard plate hold the guide ridges of the insert in position. This prevents the individual inserts from rotating – ensuring the feed ports on the wells remain open. The guide ridges center also the insert in the well. This allows media changes without cell disruption.

Guide grooves in the standard 24-well plates

Two plates

24-well standard plate

The plate includes 24 individually fillable wells that can be fitted with strips of 4 inserts and/or individual inserts.

Plate specifications

n Manufactured according to the requirements in ANSI/SLAS Standards 1 and 4

n The standard 24-well plate and 6-well special plate both have lids with condensation rings

n pureGradeTM S (untreated) and cellGradeTM plus (hydrophilic cell culture) surfaces

Automated filling of the wells for tissue culture (Photo © Fraunhofer IPA)

For efficient operations!

Feeding portGuide ridge

Automated in vitro preparation of human tissues

Two insert str ips

Insert strips with special inlet channels (the Inlet Opening System*)

For automated in vitro preparation of human tissues. The Inlet Opening System* enables rapid, consistent changing of media, from submersion culture to air-lift culture. The special inlet channels enable adjustment of the medium level without damage to the skin model. *Patent pending

Insert strips, smooth-walled

For standard applications such as secretion studies, co-culture, migration/invasion assays studies, chemotaxis tests, etc.

Insert strip specifications

n Hanging inserts – distance from the well bottom to the insert membrane is 4 mm

n cellGradeTM plus (hydrophilic cell culture) surface

n Individually packaged strips in deep-drawn cups or as a combination with 6 strips each inserted into the 6-well cell culture plate

n Track-etched PC and PET membranes

n Four different pore sizes

Membrane / pore size application examples

Pore size Application range

0.4 µm Co-culture, transport studies, secretion studies, cell polarity studies, etc.

1.0 µm Co-culture, transport studies, secretion studies, etc.

3.0 µm Migration studies, chemotaxis studies, metastasis experiments, etc.

8.0 µmMigration studies, chemotaxis studies, metastasis experiments, etc. See also the construction of full-thickness skin models by the Fraunhofer IGB (www.tissue-factory.com)

Membrane pore size and density

Properties PC PET

Optical properties translucent transparent

Cell visibility under Light Microscopy – +

Membrane thickness

Pore size 0.4 µm 10 µm 12 µm

Pore size 1.0 µm 11 µm 12 µm

Pore size 3.0 µm 9 µm 15 µm

Pore size 8.0 µm 7 µm 23 µm

Pore density (pores per cm²)

Pore size 0.4 µm 1 x 108 2 x 106

Pore size 1.0 µm 2 x 107 2 x 106

Pore size 3.0 µm 2 x 106 6 x 105

Pore size 8.0 µm 1 x 105 6 x 104

Comparison of individual insert vs. individual well in the 24-well plate

Individual insert Individual well

Growth surface 59 mm2 184 mm2

Maximum volume 772 µl 3.1 ml

Four pore sizes

photo © Fraunhofer 1GB

Full thickness skin model

Order ing in format ion

BRANDplates® Microplates24-well standard plates or 6-well plates

PS. pureGrade™ S or cellGrade™ plus surface, sterile, includes lid with condensation rings.

Description Surface Pack of Cat. No.

24-well standard plate pureGrade™ S 10 (individually wrapped, with lid) 782880

6-well plate pureGrade™ S 10 (individually wrapped, with lid) 782881

24-well standard plate cellGrade™ plus 10 (individually wrapped, with lid) 782890

6-well plate cellGrade™ plus 10 (individually wrapped, with lid) 782891

BRANDplates® Inser t St r ipsInsert Strips, smooth-walled or with inlet channels (Inlet Opening System*)

PS. cellGrade™ plus surface, sterile. Strips of 4 inserts (divisible).

Description Pore size µm

Pack of PC membraneCat. No.

PET membraneCat. No.

smooth-walled 0.4 12 (individually wrapped) 782800 782810

1 12 (individually wrapped) 782820 782830

3 12 (individually wrapped) 782840 782850

8 12 (individually wrapped) 782860 782870

with Inlet Opening System* 0.4 12 (individually wrapped) 782801 782811

1 12 (individually wrapped) 782821 782831

3 12 (individually wrapped) 782841 782851

8 12 (individually wrapped) 782861 782871

BRANDplates® Inser t System6-well plates filled with 6 insert strips

PS. cellGrade™ plus surface, sterile. Insert strips, smooth-walled or with inlet channels (the Inlet Opening System*) includes lid with condensation rings.

Description Pore size µm

Pack ofPlates with lid

PC membraneCat. No.

PET membraneCat. No.

smooth-walled 0.4 5 (30 insert strips) 782802 782812

1 5 (30 insert strips) 782822 782832

3 5 (30 insert strips) 782842 782852

8 5 (30 insert strips) 782862 782872

with Inlet Opening System 0.4 5 (30 insert strips) 782803 782813

1 5 (30 insert strips) 782823 782833

3 5 (30 insert strips) 782843 782853

8 5 (30 insert strips) 782863 782873

n Non-cytotoxic according to ISO 10 993-5, endotoxins-free (< 0.01 EU/mL), free from DNA, DNase, and RNase.

n Sterile according to ISO 11 137 and AAMI Guidelines, SAL 10-6

* Patent pending

* Patent pending

BRANDplates® and BRAND® are trademarks of BRAND GMBH + CO KG, Germany.

BrandTech® is a trademark of BrandTech® Scientific, Inc.

Other reproduced brands are the property of the respective owner.

Our technical literature is intended to inform and advise our customers. However, the validity of general empirical values, and of results obtained under test conditions, for specific applications depends on many factors beyond our control. Please appreci-ate, therefore, that no claims can be derived from our advice. The user is responsible for checking the appropriateness of the product for any particular application.

Subject to technical modification without notice. Errors excepted.

Toll Free: 888-522-2726 www.brandtech.com