Institut of plant biochemistry

L‘interaction non-hôte Persil-Phytophthora sojae, un modèle pour étudier la réponse immunitaire innée chez les plantes

TSGPTS

Open reading frame

RACE clones

cDNA clone

Elicitor activity

+-+--+---

polypeptidesexpressed fromsubclones

Elicitor-activeoligopeptide

TS

C X

X

P H

H N

C C

VWNQPVRGFKVYE

Pep-13, a minimal elicitor motif within GP42

Institute of Plant Biochemistry

Proteine ProteineGln C S + NH 2R

Cys

OGln C NH

R

O

Enzym e

Enzym e

SH

Cys

Protein Protein Gln C S

Cys

OGln C

NH 2

O SH

Cys

Enzym eEnzym e

NH 3

+

Catalytic m ode of action of transglutam inases

R = Am ines, Lysine

Institut of plant biochemistry

GP42 TGase homologs are highly conserved among the genus Phytophthora

Institute of Plant Biochemistry

IPBInstitute of Plant Biochemistry

Pep-13-containing GP42 TGase homologs are highly conserved among Phytophthora spp.

Hydropathy plot of Phytophthora sojae transglutaminasePep-13 is part of a surface-exposed loop structure

IPBInstitute of Plant Biochemistry

TGase and elicitor activities of P. sojae wild-type and mutant proteinsPep-13 is functionally indispensable

IPBInstitute of Plant Biochemistry

Pep-13 elicits defense reactions in potato with the same signal specificity as in parsley

P234A W231A

Institute of Plant Biochemistry