INTERFERON-BASED INTERFERON-BASED VETERINARY PREPARATIONSVETERINARY PREPARATIONS

Belarusian State UniversityBelarusian State UniversityMinsk, BelarusMinsk, Belarus

20122012

We propose a new class of veterinary We propose a new class of veterinary preparationspreparations,, based on recombinant based on recombinant

proteins – interferons (IFNs).proteins – interferons (IFNs).

The key characteristic of our preparations is that active The key characteristic of our preparations is that active ingredients (interferons) are identical to proteins, which ingredients (interferons) are identical to proteins, which are produced in animal’s organism after onset of are produced in animal’s organism after onset of infectious process. There is no need to inject foreign infectious process. There is no need to inject foreign substances in the organism and You can treat, for substances in the organism and You can treat, for example, horse or chick using equine or chicken alpha example, horse or chick using equine or chicken alpha IFN, respectively.IFN, respectively.Recombinant IFNs (chicken, equine, bovine etc.), Recombinant IFNs (chicken, equine, bovine etc.), containing in our preparations, are produced in containing in our preparations, are produced in Escherichia coliEscherichia coli cells using recombinant DNA technology. cells using recombinant DNA technology.

Originality and novelty of our developments are protected Originality and novelty of our developments are protected by patents in Belarus and EurAsEC.by patents in Belarus and EurAsEC.

What is interferonWhat is interferon??Interferons are a class of multifunctional proteins, produced in human or animal organism in response to entry of different viruses or another pathological factors.

Three types of molecules:Type I IFNs: -alpha(α), -beta(β), -delta(δ), -epsilon(ε), -kappa(κ), -omega(ω), -tau(τ), -zeta(ζ), etc.Type II IFNs: -gamma(γ)Type III IFNs: -lambda(λ)

Main activities of IFNs:

IFNs

Antibacterial

Antiviral

Immunomodulatory

Antiproliferative

Antiparasitic

Cells that secrete IFN have pattern-recognition receptors (PRRs) to detect molecules associated with infection. Once secreted, IFN binds to the receptors on neighbouring uninfected cells (as well as on the initial infected cell) and activates an intracellular signalling cascade, leading to upregulation of several hundred IFN-responsive genes, many of which have direct or indirect antiviral action.

Main effects, caused by injection of Main effects, caused by injection of interferon-based preparationsinterferon-based preparations

0

5

10

15

20

25

Bactericidal activity of bloodserum, %

Before injection

After 24 h

0

5

10

15

20

25

30

Lysozyme activity ofblood serum, %

Before injection

After 24 h

Main effects, caused by injection of Main effects, caused by injection of interferon-based preparationsinterferon-based preparations

0

5

10

15

20

25

30

35

T-lymphocytes, %

Before injection

After 24 h

0

5

10

15

20

25

30

35

B-lymphocytes, %

Before injection

After 24 h

Species-specificitySpecies-specificity of interferonsof interferons

Chicken IFN ≠ Bovine IFN

Interferon-based preparationsInterferon-based preparations

Interferon-alpha Interferon-alpha (equine, chicken, (equine, chicken, bovine etc.)bovine etc.)

Viral diseasesViral diseases ImmunomodulatorImmunomodulator Stress conditionsStress conditions

Interferon-alpha + Interferon-alpha + antibiotics antibiotics (gentamycin(gentamycin,,lincolinco--mycinmycin, enrofloxacin, , enrofloxacin, tylosintylosin))

Mixed infectionsMixed infections ImmunomodulatorImmunomodulator

Interferon-alpha + Interferon-alpha + vitamins (A, Dvitamins (A, D33, E, , E,

C)C) HHypovitaminosisypovitaminosis Viral diseasesViral diseases ImmunomodulatorImmunomodulator

Recombinant bovine IFNRecombinant bovine IFNRecombinant equine IFNRecombinant equine IFN

Main effects:Main effects:1 – 1 – antiviral activityantiviral activity;;2 – 2 – inhibition of growth and development of intracellular inhibition of growth and development of intracellular pathogens (pathogens (chlamydiachlamydiae, e, rickettsiarickettsia, , protozoaprotozoa, etc.), etc.);;3 - 3 - increase of lysozyme activity of blood serumincrease of lysozyme activity of blood serum;;4 – 4 – increase of bactericidal activity of blood serumincrease of bactericidal activity of blood serum;;5 – 5 – antitoxic and antistress effectantitoxic and antistress effect;;66 – – macrophages stimulation and increase of phagocytosismacrophages stimulation and increase of phagocytosis;;77 – – increase of antibodies production increase of antibodies production;;88 – – activation of native killers activation of native killers;;

Interferon-alpha + vitaminsInterferon-alpha + vitamins

Vitamin A takes part in important oxidative processesVitamin A takes part in important oxidative processes

Vitamin D3 takes part in regulation of energy and mineral Vitamin D3 takes part in regulation of energy and mineral metabolism metabolism

VitaminVitamin Е Е influences on metabolism of proteins, influences on metabolism of proteins, carbohydrates and mineralscarbohydrates and minerals

Vitamin C increases protective functions of the organismVitamin C increases protective functions of the organism

Recombinant IFN-alpha acts as protective, Recombinant IFN-alpha acts as protective, immunomodulatory and antistress factor; it immunomodulatory and antistress factor; it possesses possesses antiinflammatory activityantiinflammatory activity and increases and increases

resistance of the organism.resistance of the organism.

Residual quantitiesResidual quantities

Milk and meat – no Milk and meat – no limitations!!!limitations!!!

Interferon-alpha + antibiotics (gentamycin,Interferon-alpha + antibiotics (gentamycin, lincomycin, enrofloxacin, tylosin)lincomycin, enrofloxacin, tylosin)

Recombinant interferon-alpha, containing in preparations, provides:Recombinant interferon-alpha, containing in preparations, provides:non-specific prolonging antiviral defensenon-specific prolonging antiviral defense;;inhibition of growth and development of intracellular pathogens inhibition of growth and development of intracellular pathogens ((chlamydiachlamydiae, e, rickettsiarickettsia, , protozoaprotozoa, etc.), etc.);;immunostimulatory and antistress effectimmunostimulatory and antistress effect;;increase of lysozyme and bactericidal activity of blood serumincrease of lysozyme and bactericidal activity of blood serum;;

• manyfold increase of antibacterial activity of antibiotics and manyfold increase of antibacterial activity of antibiotics and simultaneous elimination of their immunodepressive effect on simultaneous elimination of their immunodepressive effect on animal’s organism!animal’s organism!

Antibiotic, containing in preparation, providesAntibiotic, containing in preparation, provides::having increased lysozyme and bactericidal activity of blood serum having increased lysozyme and bactericidal activity of blood serum due to the interferon action, antibiotic in low therapeutic due to the interferon action, antibiotic in low therapeutic concentrations ensures full and rapid elimination of sensitive concentrations ensures full and rapid elimination of sensitive pathogenic bacteriapathogenic bacteria;;developed formulation provides prolonging mode of action of developed formulation provides prolonging mode of action of antibioticsantibiotics;;no immunodepressionno immunodepression;;due to the complex action of interferon and antibiotic there is dramatic due to the complex action of interferon and antibiotic there is dramatic minimization of risk associated with formation of resistant strains.minimization of risk associated with formation of resistant strains.

Thanks for your attention!Thanks for your attention!