introduction rhizobium, has the ability to fix atmospheric nitrogen in symbiotic association with...
TRANSCRIPT
INTRODUCTION
Rhizobium, has the ability to fix atmospheric nitrogen in symbiotic association with host legumes.
Rhizobia invade the roots of legume plants through root hairs, form effective pink colored nodules in the tap or lateral roots and lives symbiotically inside the nodules to fix nitrogen to plant accessible forms
It was estimated that a total of 175 million metric tones of N is fixed per year globally through biological nitrogen fixation involving Legume Rhizobium symbiosis.
Rhizobium as component of INM Rhizobium as component of INM
Rhizobium as component of INM Rhizobium as component of INM
Rhizobium, a root nodule
bacterium, has the ability to fix
atmospheric nitrogen in symbiotic
association with host legumes.It
was estimated that a total of 175
million metric tones of N is fixed per
year globally through biological
nitrogen fixation involving
Legume Rhizobium symbiosis.
• Curling of root hair• Invagination point• Infection thread develop• Bacteria released • Nodular tissue develop
HOW RHIZOBIA INFECT LEGUME ??
HOW RHIZOBIA INFECT LEGUME ??
Rhizobium as component of INM Rhizobium as component of INM
Selection of legume cropsSelection of legume crops Collection of nodulesCollection of nodules
ISOLATION AND MAINTENANCE OF RHIZOBIUM
Rhizobium isolation in mediaRhizobium isolation in media Preservation in slantPreservation in slant
Purified rhizobial cellsPurified rhizobial cells Nodulation testsNodulation tests
Selection of legume cropsSelection of legume crops Collection of nodulesCollection of nodules
Preservation in slantPreservation in slant
Selection of legume cropsSelection of legume crops Collection of nodulesCollection of nodules
Rhizobium isolation in mediaRhizobium isolation in media Preservation in slantPreservation in slant
Selection of legume cropsSelection of legume crops Collection of nodulesCollection of nodules
Purified rhizobial cellsPurified rhizobial cells
Rhizobium isolation in mediaRhizobium isolation in media Preservation in slantPreservation in slant
Selection of legume cropsSelection of legume crops Collection of nodulesCollection of nodules
Nodulation testsNodulation testsPurified rhizobial cellsPurified rhizobial cells
Rhizobium isolation in mediaRhizobium isolation in media Preservation in slantPreservation in slant
Selection of legume cropsSelection of legume crops Collection of nodulesCollection of nodules
CHARACTERISTICS OF RHIZOBIUM
Rhizobia invade legume roots through root hairs
Form effective pink colored nodules in the roots
Lives symbiotically inside the nodules and fix nitrogen
Converts atmospheric nitrogen to plant accessible forms
Most rhizobia are host specific in nature
Rhizobia invade legume roots through root hairs
Form effective pink colored nodules in the roots
Lives symbiotically inside the nodules and fix nitrogen
Converts atmospheric nitrogen to plant accessible forms
Most rhizobia are host specific in nature
Rhizobium as component of INM Rhizobium as component of INM
50 – 200 KG NITROGEN PER HECTARE PER CROP
50 – 200 KG NITROGEN PER HECTARE PER CROP
Rhizobium as component of INM Rhizobium as component of INM
Rhizobium Inoculation and Crop Response
Inoculation Method
Nitrogen
Fixation
Rhizobium culture
Crop Response
Contribution of Rhizobium in biological N fixation
CropCrop
RhizobiumRhizobium species species Quantum of N fixed Quantum of N fixed (kg N/ha/yr)(kg N/ha/yr)
SoybeanSoybean R. japonicumR. japonicum 60-8060-80
AlfalfaAlfalfa R. melilotiR. meliloti 100-200100-200
CloverClover R. trifoliiR. trifolii 100-200100-200
GroundnutGroundnut R. sp.R. sp. 50-6050-60
PeaPea R. leguminosarumR. leguminosarum 52-7752-77
CowpeaCowpea R. sp. R. sp. 80-8580-85
Green/ Black gramGreen/ Black gram R. leguminosarumR. leguminosarum 50-5550-55
Bengal gramBengal gram Bradyrhizobium sp.Bradyrhizobium sp. 85-11085-110
Rhizobium as component of INM Rhizobium as component of INM
LET US SUM UP
Rhizobium fix atmospheric nitrogen in symbiotic
association with host legumes.
It was estimated that a total of 175 million metric
tonnes of N is fixed per year through legume-Rhizobium
symbiosis.
Rhizobium lives symbiotically inside the nodules and fix
nitrogen which is converted to plant accessible forms.
Contd…..
Rhizobium as component of INM Rhizobium as component of INM
Rhizobia invade the roots of legume plants through root
hairs and form effective pink colored nodules in the tap
or lateral roots.
Most Rhizobia are host specific and nodulate specific
legumes.
Rhizobium as component of INM Rhizobium as component of INM
LET US SUM UP