Simultaneous transplantation of co-cultured mesenchymal stem cells and skeletal myoblasts improves ventricular function in Chagas disease

Luiz Csar Guarita-Souza MD PhD

PUCPR - Experimental Laboratory of Cell Culture Institute of Biological and Health Sciences Curitiba-Pr-Brazil

IntroductionSkeletal Myoblasts cells diferentiationMyocardial ScarSkeletal FibersGuarita-Souza LC, Int J Cardiol. 2006 Aug 28;111(3):423-9

NeoangiogenesisIntroductionMesenchymal Stem cells diferentiationGuarita-Souza LC, Transplant Proc. 2006Jul-Aug;38(6):1953-4

SkeletalMyoblastMesenchymalStem CellsAngio-muscularRegeneration+ Introduction Myocardial Cell Therapy

Arq. Bras. Cardiol.2004, OctOct;83(4):294-9 The Association of Myoblast and Mesenchymal Stem Cells Transplantation, on Post Infarct Ventricular Dysfunction

Guarita-Souza LC; Carvalho KAT, Rebelatto C; Senegaglia A, Myiague N; Furuta M; Francisco J, Olandowski M, Hansen P. Introduction Myocardial Cell Therapy

Cell Therapy in Myocardial Infarction SegmentarMyocardial Scar and Ischemic

Both Cell Therapy in a Cardiomyopathy non-ischemic ? Introduction Cell Therapy

Evaluated the histological and functional effects of simultaneous autologous transplantation of co-cultured mesenchymal and skeletal myoblasts cells in an experimental model of dilated cardiomyopathy due to Chagas disease.Objective

TransmissionTriatoma Infestans: 5 to 14 days:sains and symptomsTransfusional: 30 to 40 days symptomsChronic form: after 10 years

Immunologic Activity:Humoral and Cellular Immunity Introduction Chagas Disease FisiopathologyTriatoma InfestansBarbeiro

Forms of DiseaseAcuteIndeterminated60% Cardiac30% after 10-20yDigestiveBothCongenital

Trypanossoma Cruzi Myocardial InfectionIntroduction Chagas Disease Fisiopathology

Chronic myocarditeChronic inflamatory processdiffuse fibrosisImpairment of microcirculationIschemic myocardium HE, 200xIntroduction Chagas Disease PathologyChronic inflamatory processMyocardial scar

MethodsProtocol of ResearchEco 1MAnimalsinoculationTX Histology

EcoBaseline

7 days14 of dayscultive8 M MuscularBiopsyBone MarrowAspiartion1 MonthT Cruzi 150.000 forms

Cell Therapy in Myocardium Protocol of Cell Culture

Material and Method 80 animals were inoculated - 200 gramas 21.25% - 17 animals included - FE < 37%

Co-culture group: 9 animals mesenchymal stem cellsskeletal myoblast cells0.30 ml - 5,4x106 8,0x106 cells

Control group : 8 animalssaline - 0.30 ml

Material and MethodEchocardiographic studyBi-Dimensional Echocardiographic study HP/SONOS 5500- Linear Transduction of 15MHzAfter eigth months of inoculationOne month after transplantation

Parameters AnalysisLVEDV (ml)LVESV (ml)LVEF (%)

Results Echocardiographic study

ResultsLVEF(%)(*) Paired Students test p

Results LVESV (ml)(*) Paired Students test p

Results LVEDV (ml)(*) Paired Students test p

Results Pathology

HE, 200X Control groupTreat groupResultsPhatology

ResultsPhatology Gomery Trichrome Treat group 200X 400X 400X

Results Phatology / Immunostained Fast Miosin, 40xControlTreat group

The co-transplant of mesenchymal stem cell and skeletal myoblasts cell is functionally effective in the Chagas disease ventricular dysfunction.Conclusion