Introduction to Biotechnology

Instructor: Jennifer Keelen-Lazare

Email: jlazare@austinis.org Phone 512-841-1572

Website:

Grade Website: grades will be online in gradespeed

Course Descrition: An introduction to biotechnology including career exploration, history and applications of DNA/RNA technology molecular biology, bioethics, and laboratory safe practices. The course is supplemented with laboratory exercises, demonstrations and field trips that illustrate the basic techniques of biotechnology including laboratory topics and finally the course concludes with a consideration of bioethical issues relating to this powerful new technology.

Supplies: Textbook-Introduction to Biotechnology Lab Notebook Internet Access outside of class 1 role of paper towels 1 ream of paper

Grades: Access online @ www.mygradebook.com Test-50% Labs, homework, everything else-50%

Enrollment: Everyone will receive Science Credit-Honors You may also enroll in this course for DUAL CREDIT (college and high school

credit)o I will help you enroll in Early College Start in the Fallo I will help you enroll in BITC 1311 in the Spring

Projects for this course (all are test grades unless indicated): Exploravision Comepetition (Fall Final Exam) Stockmarket Game Case Studies DNA Essay Contest Bio-Dreaming Poster Entry Thinkquest Competiion CSI Blogging

Field Trip Reports

COURSE OUTLINE LABS

MODULE 1 - BASIC SKILLS

View Detailed Module Outline (pdf) A. What is Biotechnology

1. History of biotechnology2. Overview of modern biotechnology

B. Math Skills1. exponents2. scientific notation3. decimal computation4. decimal conversions5. dimensional analysis

C. Lab InstrumentationD. Making solutions

1. molarity

2. dilutions

Student Labs 1. Safety (pdf) 2. Documentation (pdf) 3. Math Skills (pdf) 4. Measuring with a

Micropipet (pdf) 5. Calibrating Instruments

(pdf) 6. Solutions (pdf) 7. Solutions Extension (pdf)

1.

MODULE 2 - BASIC DNA

View Detailed Module Outline (pdf) A. Job of DNAB. DNA moleculeC. ReplicationD. TranscriptionE. TranslationF. Gene Expression

G. RNA

Student Labs 1. DNA Extraction (pdf) 2. DNA From Mouse Tail

Extension (pdf) 3. Extraction Comparison (pdf) 4. Extraction Overhead (pdf)

MODULE 3 - DNA TECHNOLOGY

View Detailed Module Outline (pdf) A. Storage and Handling of DNAB. Restriction enzymesC. Recombinant DNA D. DNA librariesE. Gel electrophoresis

F. DNA sequencing

Student Labs 1. Restriction Analysis - New

Lab (pdf) 2. pGlo Transformation Kit

(pdf) 3. GFP Purification (pdf) 4. Bioinformatics Activity

(pdf)

5. Electrophoresis Lab (pdf)

MODULE 4 - DNA ANALYSIS

View Detailed Module Outline (pdf) A. Methods of Analysis

1. RFLP2. DNA probes

Student Labs 1. PCR Alu (pdf) 2. PCR Review (pdf) 3. PCR Review Key (pdf)

3. PCR4. DNA ArraysB. Diagnosing Infectious Disease

1. HIV, Tuberculosis, etc.C. Identifying genetic disease

1. Cystic fibrosis, Huntington’s disease, etc.

2. Ethics of genetic testingD. Forensics

E. Human Genome Project

4. DNALC PCR Info Lab (pdf)

MODULE 5 - PROTEINS

View Detailed Module Outline (pdf) A. Structure and Function

1. Amino acids2. Structure (1°, 2°, 3°, 4°) Denaturing

B. Enzymes C. Separation and Analysis

1. Gel electrophoresis2. Column Chromatography

D. Storage and Handling of Proteins

Student Labs 1. Column Chromotography

(pdf) 2. Column Chromotography

Extension (pdf) 3. Ultra Bad Hair Day Intro

(pdf) 4. Ultra Bad Hair Day SOP

(pdf) 5. Ultra Bad Hair Day

Questions (pdf) 6. Beano Lab (pdf)

MODULE 6 - MICROBES AND THE ENVIRONMENT

View Detailed Module Outline (pdf) A. Environmental biotechnology

1. Bioremediation2. Biodegradation

B. Gaia conceptC. Biogeochemical cyclesD. Soil microbiologyE. The waste stream

1. Breakdown of wastes2. Waste disposal3. Hazardous wastes

F. Pollution cleanupG. Monitoring environmental healthH. H. Microorganisms and mining

I. I. Ethics of releasing microorganisms into the environment

Student Labs 1. Microbes in the

Environment (pdf) 2. Biodegradation (pdf) 3. Enrichment for Pollution

Lab (pdf) 4. Selecting Soil Organisms

(pdf)

MODULE 7 - MEDICAL BIOTECHNOLOGY

View Detailed Module Outline (pdf) Student Labs

A. Pharmaceutical products of biotechnology1. Human replacement proteins2. Immune Therapy

B. Gene therapy

C. Human Genome project outcomes

1. Immunoassay (pdf) 2. ELISA Test (pdf) 3. ELISA Quickguide (pdf)

MODULE 8 - AGRICULTURAL BIOTECHNOLOGY

View Detailed Module Outline (pdf) A. DNA Insertion methodsB. Enhancing Resistance in plantsC. Bioengineered foodD. Bioengineered animalsE. Transgenic animalsF. Clones

G. Ethics

Supplements 1. Cloned Animals Article

(pdf)

2. Grains of Hope (pdf)

MODULE 9 - THE BUSINESS OF BIOTECH

View Detailed Module Outline (pdf) A. Careers in biotechnology B. From discovery to marketing

C. Company structure

Student Labs 1. Career Entire Module (pdf)