introduction to training agenda product overview what’s new from prostar 325 hardware overview...
TRANSCRIPT
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Introduction to training
Agenda• Product overview
• What’s new from ProStar 325
• Hardware overview
• Initialization routine
• Software CD overview
• Detector installation & PolyVerfiy
• PolyDiagnostics
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Product Overview
Based on the ProStar 325 with many shared parts
Two models available:
• Single 1024 array • Dual 1024 array
Control by both Star with PolyView 2000 and Galaxie(PolyView is now included with Star driver)
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Product Overview - workstation control
Galaxie• Four 335 DADs per acquisition server
• Driver included in Varian LC control drivers
Star• two 335 DADs per Star Workstation
• Driver included in LC control option
• Installation of driver automatically installs PolyView 2000
Ethernet communications best with a switch (not a hub)
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Product Overview
• Same cabinet• Same UV & visible lamps• Same flow cells• Same rear panel, connectors, interface boards & cables• Same communications protocol• Same power supply• Same Main pwb (difference in firmware)
ProStar 335 and 325 similarities
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What’s New
Same look as ProStar 325 except no PPC capabilities
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What’s New
Dual Array• 1024 photodiode sample and reference array
• Extended range spectra
• Used for Analytical to Prep Applications
Single Array• 1024 photodiode sample array
• 2 photodiode references (250 and 500 nm)
• No extended range
• Uses 9x0 and 4x0 flow cells only
Different optics module
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Hardware Overview – optics module
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Product Overview – ray trace diagram
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Hardware Overview - Polychromator
Available slit widths
1nm2nm4nm8nm
16nm
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Hardware Overview – dual path flowcell
Illustration is of a 9x1 flowcell
sample
reference
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Hardware Overview – optics module
Wavelength calibration data must be entered into the EEPROM using Diagnostics software.
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Electronics – wavelength calibration factors
Use the floppy disk to update the EEPROM
automatically
This label will be attached to the top of each optics module
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Hardware Overview – Electronics
Only difference between 335 & 325 is firmware
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Hardware Overview - Electronics
Add new MAC address label when swapping pwb’s
New MAC address label comes with each new PWB
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Hardware Overview – initialization routine
• Peak search for 656.1nm deuterium emission line
• Determines dark current table (0%T)
• Gain (Lamp) calibration is now user driven. Stored in non-volatile memory.
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Initialization
Status LED’sinitialized
not initialized
Hardware fault
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Software CD overview
Software CD ships with each ProStar 335 detector.
Part number: 85 102114 00
Auto-loading CD which contains:• Galaxie drivers and manuals• Star driver and manuals• PolyVerfiy software• PolyDiagnostics
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Software CD overview
Customer software CD 85-1xxxxx-00 will contain
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Software CD overview – installation PolyVerify
Use PolyVerify to check the performance of the ProStar 335
After installing, a desktop icon will be created called
ProStar
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Software CD overview - installation PolyVerify
Only two test are required at installation;
Noise/Drift Wavelength
accuracy
IQ/OQ is also availablepart number: 03-914756-00;4
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Software CD overview – installation PolyVerify
Print customer certificate
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The ProStar 335 must be initialized and have both lamps on for at least one hour prior to running any tests.
Noise test : pump water for at least 10 minutes at a pump head pressure of at least 100 atm using a back pressure restrictor of 40psi.
Wavelength accuracy: same conditions as the Noise test.
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Software CD overview - PolyDiagnostics
Browse the CD using Windows Explorer.
Diagnostics is not self loading and not intended for
customer use.
Installing PolyDiagnostics
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Software CD overview - PolyDiagnostics
To run PolyDiagnostics you will need to copy these three files to the ProStar directory where PolyVerify is installed. From there double click the “PolyDiagnostics.exe” file using Windows Explorer.
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Software CD overview - PolyDiagnostics
Similar appearance to
325 Diagnostics
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Software CD - PolyDiagnostics
Service folder also contains the firmware updater program
Use the Diagnostics program to update the EEPROM and perform various calibration routines i.e. wavelength & lamp calibrations
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Software CD - PolyDiagnostics
The EEPROM contents will need updating whenever;
• the Main PWB is replaced• the optics module is replaced• to update installation & maintenance dates
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Software CD - PolyDiagnostics
EEPROM contents;
• Detector serial number• MAC address• Wavelength calibration factors, slope and offset• IP address• Gateway address• Subnet mask• Lamp timer (on/off date and times)• Detector installation date• Last maintenance date• Error log• Hg wavelength calibration date
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Software CD - PolyDiagnostics
When to calibrate
Performing a Lamp Calibration will help reduce noise and improve linearity.
• Deuteriunm lamp is changed• Visible lamp is changed• Every 1000 lamp source hours• Increasing signal noise• When changing flowcell to one of a different pathlength