introduction/microscope intro/cell basics/cell division · magnification demo (pin head) x30,000....
TRANSCRIPT
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Introduction/Microscope
Intro/Cell Basics/Cell Division
A215 Laboratory
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Lab Organization
• Cadaver Days
– Focus on the gross structures of the body
• Scope Days
– Focus on the histology component of lab
• PDFs of intros can be downloaded from
http://www.indiana.edu/~anat215/lab
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What is Histology?
• Histology=Microscopic Anatomy
– Learn what makes up the different tissues
in the body
– Learn how different tissues relate to
structure and function (or malfunction)
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Types of Microscopy
• Electron Microscopy
– Powerful magnifying technique used to
visualize intracellular structures
– Up to 105x
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Magnification Demo
(Pin Head)
x33
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Magnification Demo
(Pin Head)
x100
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Magnification Demo
(Pin Head)
x200
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Magnification Demo
(Pin Head)
x250
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Magnification Demo
(Pin Head)
x500
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Magnification Demo
(Pin Head)
x1000
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Magnification Demo
(Pin Head)
x5000
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Magnification Demo
(Pin Head)
x15,000
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Magnification Demo
(Pin Head)
x30,000
![Page 14: Introduction/Microscope Intro/Cell Basics/Cell Division · Magnification Demo (Pin Head) x30,000. Types of Microscopy • Electron Microscopy – Powerful magnifying technique used](https://reader030.vdocuments.net/reader030/viewer/2022041112/5f172e70a4dc707aaf5b4109/html5/thumbnails/14.jpg)
Types of Microscopy
• Electron Microscopy
– Powerful magnifying technique used to visualize
intracellular structures
– Up to 105x
• Light Microscopy
– Thin section of preserved tissue is cut and placed
on a slide and stained
– 4x-1000x
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Types of sectioning
Cross Section Longitudinal Oblique
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Microscope Introduction
• Pp. 4-5 in Lab manual
• Turn light intensity between 1/2 and
• Make sure you can see pointer in RIGHTeyepiece
• Turn objectives using rubber disc, NOTbarrels
• DO NOT use the 100x objective!
• Use “coarse focus” for 4x objective only
• Use “fine focus” for 10x-40x objectives
Kidney Slide Ureter Slide
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Cell Basics
Cell Membrane
Nucleus
Cytoplasm
Nucleolus
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Cell Basics
• Not all cells look the
same
• Cells can vary in
terms of visible
organelles and
shape
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Available on
reserves at Life
Science Library or
http://ereserves.indiana.e
du/eres/coursepage.aspx
?cid=1260&page=docs
Cell
Organelles
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Rough
Endoplasmic
Reticulum
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Cell
Organelles
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Mitochondria
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Centrioles
9 sets of
triplets
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Microvilli
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Cilia
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Cilia
9 doublets
+2
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Cell Division
Prophase
“Prepare”
Metaphase
“Meet”
Anaphase
“Apart”
Telophase
“Two”