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International Seminar iPROMISE
28th and 29th November 2017
Integrating Omics Precision Medicine
Student Activity Room I & II,
1st Floor FF2 building, UiTM Puncak
Alam
iPROMISE International Seminar 2017
28-29th November 2017, UiTM Puncak Alam
iii
Content
Message from the Director of iPROMISE ..................................................... iv
Message from the Organising Chairman ...................................................... v
Organizing Committee ................................................................................... vi
Programme Tentative .................................................................................... ix
Scientific Program (Oral Presentation) ........................................................ xi
Bibliography of Professor Dr Mohammad Safiqul Islam.............................. 1
Invited Speaker’s Abstract ............................................................................. 3
Postgraduate Student’s Abstract ................................................................ 13
Acknowledgement ........................................................................................ 28
Sponsor ......................................................................................................... 29
iPROMISE International Seminar 2017
28-29th November 2017, UiTM Puncak Alam
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Message from the Director of iPROMISE
First and foremost, I would like to express my deepest
gratitude to the iPROMISe 2017 organizing committee
on their monumental and tireless effort in realising this
seminar. It is therefore a great pleasure for me to
welcome all the distinguished guests, speakers and
participants to the iPROMISE international Seminar
(iPROMISe 2017). It is also a huge honour for me to
welcome our special guest of honour Prof. Dr.
Mohammad Safiqul Islam from Noakhali Science and
Technology University, Bangladesh to our humble
cradle of knowledge. I sincerely hope that this seminar
will serve as an important platform for the exchange of
idea, social networking and research collaboration at the international level.
Designating “Integrating Omics in Precision Medicine” as the theme has been
appropriate and well-timed. The world is facing an arduous challenge in the healthcare
industry in terms of accessibility, feasibility and affordability. We believed that precision
medicine is the key, hence it is our duty to create awareness so that everybody is ready
to play their part in making a smooth transition for a better quality of healthcare in the
future. With increasing medical bill looking like an insurmountable task, there is a dire
need to revamp our perspective of delivering health care, to modernize it so that the
public can benefit from the advancement of technology. Innovative researches are
accelerating at various levels, therefore a more concerted collaborative effort from all
parties is crucial so that we can serve the public the best way possible.
It is my aspiration that such event can be continued in the future, discussing and
creating awareness on cutting edge technology with the hope that brighter prospects are
not too distant from us.
Thank you,
Professor Dato’ Dr. Mohd Zaki Salleh
Director
Integrative Pharmacogenomics Institute (iPROMISE)
Universiti Teknologi MARA
iPROMISE International Seminar 2017
28-29th November 2017, UiTM Puncak Alam
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Message from the Organising Chairman
It is with great pleasure that we welcome you to the International
iPROMISE Seminar (iPROMISe 2017). The seminar is a
continuum of our commitment to engage researchers, academics,
industry and the community on issue of global health. The theme
of the seminar “Integrating Omics in Precision Medicine” was
selected in line with the Malaysia Education Blueprint 2015-2020.
True to the theme, iPROMISe 2017 integrates an innovation
competition, as well as an exhibition of interdisciplinary research
groups into the scientific programme.
Our keynote lecture “Pharmacogenomics: The Way to
Personalised Medicine” by Professor Dr. Mohammad Safiqul
Islam will highlight examples of transformation of healthcare,
through the use of advanced methods and technology when dealing with health data. In
addition, an impressive line-up of eminent speakers will be sharing their insights at the plenary
sessions.
We are delighted that iPROMISe 2017 has attracted significant number of participation which
represents the tremendous support. A warm thank you to our distinguished speakers and
participants for imparting scientific value to the seminar and personal gratitude to all members
of organising committee for their excellent organisation and commitment.
We hope that iPROMISe 2017 will further steer the development of research towards
improving healthcare.
Best wishes,
Mohd Izwan Mohamad Yusof
Chairman of the Organising Committee,
iPROMISE International Seminar (iPROMISe) 2017
iPROMISE International Seminar 2017
28-29th November 2017, UiTM Puncak Alam
vi
Organizing Committee
PATRON
Profesor Emeritus Dato’ Dr. Hassan bin Said
ADVISOR
Profesor Dato’ Dr. Mohd Zaki Salleh
CHAIRPERSON
Mohd Izwan bin Mohamad Yusof
SECRETARY
Rizal Husaini bin Razali
VICE SECRETARY
Puteri Nur Aliah bt Wan Faizal
TREASURER
Nur Madilawani bt Badri
iPROMISE International Seminar 2017
28-29th November 2017, UiTM Puncak Alam
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SUB COMMITTEE GROUP
Scientific Programme Bureau
Profesor Dr Teh Lay Kek
Profesor Dr. Wong Tin Wui
Y.M. Dr. Tengku Shahrul Anuar Tengku Ahmad Basri.
Dr Richard Muhammad Johari James
Dr. Mohd Salleh bin Rofiee
Dr Ang Geik Yong
Dr Yu Choo Yee
Dr Lim Wai Feng
Hisyam bin Jamari
Vinothini Subramaniam
Logistic
Muhd Hanis bin Md Idris
Mohd Nur Fakhruzzaman bin Noorizhab
Registration
Nurul Ain bt Khoruddin
Rosnani Hanim bt Mohd Hussain
Protocol
Norzuliana bt Zainal Abidin
Food & Beverages
Umi Hairun Anis bt Ismail
Suhalfarina bt Suhaimi
iPROMISE International Seminar 2017
28-29th November 2017, UiTM Puncak Alam
viii
Certificate & Gifts
Noor Fahimah bt Saari
Hazirah bt Abd Azhar
Graphic & Publicity
Muhamad Aswad bin Abd Razak
Muhammad Zaid bin Abdul Shukor
Wan Nur Izatti bt Kamarul Hisham
Technical
Muhammad Afif bin Muhammad Amin
Mohammad Faris Fauzi bin Ismail
Special Tasks
Mohammad Zulfadhly bin Jan Jam
Aina Mardhia bt Khalid
Norhuda bt Kadir
iPROMISE International Seminar 2017
28-29th November 2017, UiTM Puncak Alam
ix
Programme Tentative
Day 1: Tuesday, 28th November 2017
Venue Student Activity Room 1, 1st Floor, FF2 Building
0800-0900 Registration
0900-0915 Arrival of VIP
0915-0930 Doa recital & Welcoming Speech
0930-1030 Keynote Speaker:
Profesor Dr Mohammad Safiqul Islam “Pharmacogenomics: the Way to Personalized Medicine”
1030-1100 Morning break
1100-1130 Invited speaker 1: Dr. Yu Choo Yee
“Exploring the Human Genome: Unlocking Life’s Code”
1130-1200 Invited speaker 2: Dr. Ang Geik Yong
“Sports Genomics: Translating Potential into Performance”
1200-1230 Invited speaker 3: Vinothini a/p Subramaniam
“Basic Applied Bioinformatics”
1230-1300 Invited speaker 4: Dr.Lim Wai Feng
“Epigenetic: Food, Lifestyle & Environment = YOU”
1300-1400 Lunch Break
Concurrent session
Venue Student Activity Room 1, 1st Floor, FF2
Building Student Activity Room 2, 1st
Floor, FF2 Building
1400-1430
Invited speaker 5: Dr. Nauman Rahim Khan
“Microwave & 5-Fluorouracil Ethosomes Combination for Enhance
Skin Drug Retention”
Invited speaker 9: Dr. Lee Chee Yang
“Next Generation Sequencing (NGS) Solutions”
1430-1500
Invited speaker 6: Dr. Asif Nawaz Marwat
“Profiling of Skin Drug Delivery of Chitosan: Surfactant Nanoparticles”
Oral Presentations
1500-1530
Invited speaker 7: Dr. Kifayat Ullah Shah “Treatment of Pulmonary
Tuberculosis Using Rifampicin: Loaded Double Receptor-Targeting”
1530-1600
Invited speaker 8: Dr. Mulham Al-Fatama “Preparing & Evaluation of Alginate –
C18 Conjugate Nanoparticles Embedded Into Coated Calcium Alginate Beads for Efficient Oral
Insulin Delivery”
1600-1700 Oral Presentations
1700-1715 Tea break
iPROMISE International Seminar 2017
28-29th November 2017, UiTM Puncak Alam
x
Day 2: Wednesday, 29th November 2017
Venue Student Activity Room 1, 1st Floor, FF2 Building
0800-0900 Registration
0900-0930 Invited speaker 10: Dr. Mohd Salleh Bin Rofie
“Secret to Stay Young with Murunggai”
0930-1030 Oral Presentations
1030-1100 Morning break
1100-1130 Invited speaker 11: Hisyam bin Jamari
“Zebrafish as a Disease Model”
1130-1200 Prize & Closing ceremony
1200-1400 Lunch Break
Venue Integrative Pharmacogenomic Institute (iPROMISE) 7th Floor, FF3 Building
1400-1600 iPROMISE Open day
iPROMISE International Seminar 2017
28-29th November 2017, UiTM Puncak Alam
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Scientific Program (Oral Presentation)
Tuesday, 28th November 2017, Student Activity Room 1, FF2 Building, UiTM Selangor, Puncak Alam Campus
No. Time Name Title
1 1430-1445 Mohd Nur Fakhruzzaman Bin
Noorizhab
Multi-omics of Thermal Adaptation Strategies of Geobacillus thermocatenulatus and Some of Hot
Spring Thermophiles
2 1445-1500 Nurul Azmir Bin Amir Hasim Metabolomics Of Colorectal Cancer In Malaysia
3 1500-1515 Noor Fahimah Binti Saari Effect of Lansium domesticum Leaves Ethanolic Extract on Anxiety-Like Behaviour in Zebrafish
4 1515-1530 Hazirah Binti Azhar Quantification of Cortisol Using LC/MS/MS in Relation to Stress Among University Students
5 1530-1545 Rizal Husaini Bin Razali
Understanding the Genetic Risk of Childhood Leukemia among the Orang Asli and Malay in
Malaysia: Mining the Whole Genome Sequence Database
6 1545-1600 Umi Hairun Anis Binti Ismail Differential Protein Expression in Bone
Remodelling Induced by Denture Wearing – Preliminary Results
7 1600-1615 Rosnani Hanim Binti Mohd
Hussain
Vision Threatening Amoeba: Morphological and Molecular Based Evidence Isolated From Contact
Lens Users, Malaysia
8 1615-1630 Muhd Hanis Bin Md Idris In silico Molecular Docking Studies on Chalcone
and Flavones Derivatives as Multi-subtypes Inhibitors of Phosphodiesterase
Tuesday, 28th November 2017, Meeting room, iPROMISE, UiTM Selangor, Puncak Alam Campus (Concurrent Session)
No. Time Name Title
9 1600-1615 Dr.Kamran Ashraf Chemical Diversity Analysis In Indian Turmeric
(Curcuma longa L.) by HPTLC Method
10 1615-1630 Norul Nazilah Ab’lah Development of Resistant Corn Starch for Use as
an Oral Colon-specific Nanoparticulate Drug Carrier
11 1630-1645 Badrul Hisyam Zainudin Design of Low Molecular Weight Pectin and its
Nanoparticles Through Combination Treatment of Pectin By Microwave and Inorganic Salts
Wednesday, 29th November 2017, Student Activity Room 1, FF2 Building, UiTM Selangor, Puncak Alam Campus
No. Time Name Title
12 0930-0945 Norzuliana Binti Zainal Abidin Whole Genome Sequence Analysis of a Clinical
Multi Drugs Resistant Mycobacterium tuberculosis (MDR-TB)
13 0945-1000 Nurul Ain Binti Khoruddin Genomic Analysis of Breast Cancer Risk for the
Orang Asli and Malays
14 1000-1015 Suhalfarina Binti Suhaimi Comparative Genomic Analysis of
Mycobacterium tuberculosis in Identification of Multidrug Resistance Genes
15 1015-1030 Mohd Izwan Bin Mohamad
Yusof
Significant Modulation of Immune Response and Oxidative Stress During Infection of Klebsiella
pneumoniae Profiled Using Metabolomics Analysis
iPROMISE International Seminar 2017
28-29th November 2017, UiTM Puncak Alam
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Bibliography of Professor Dr Mohammad Safiqul Islam
Dr. Mohammad Safiqul Islam is currently working as a
Professor and Chairman of the Department of Pharmacy,
Noakhali Science and Technology University, Bangladesh and
is the principal investigator of pharmacogenomics lab. After
completing his B.Pharm and M.Pharm from the University of
Dhaka, he joined as an industrial pharmacist in the Novartis
(BD) Ltd for 2 years and later worked as a community
pharmacist in abroad. His inclination always lies toward
academia and after gaining substantial experiences in
pharmaceutical fields, he joined in the University of Asia Pacific
in 2005 and finally in the current institution in 2006 as a
lecturer. In 2010, he joined the research group of late Professor Dr. Abul Hasnat,
Department of Pharmacology and Clinical Pharmacy, University of Dhaka who was the
pioneer of pharmacogenomics research in Bangladesh and completed his PhD on the
“Lung cancer risk in relation to nicotinic acetylcholine receptor, CYP2A6 and
CYP1A1 genotype in Bangladeshi population”. Professor Dr. Ann K Daly, Newcastle
University, UK was also involved with his PhD research work and relevant publication and
one of his publications obtained the University Grants Commission (UGC) Bangladesh
award, 2013.
Professor Islam then worked on the several researches works on the pharmacogenomics
of Bangladeshi lung, breast, colorectal and prostate cancers and is now actively
collaborating with some local and international research institutes. A research article
entitled “Loss of asparagine synthetase causes congenital microcephaly and a
progressive form of encephalopathy” was published from the collaboration with a
Canadian research group in the Neuron. He has also collaboration with a faculty member
of Monash University Malaysia and recently an article has been published in the BMJ
Open from this collaboration.
He received a grant from the Ministry of Science and Technology, Bangladesh in 2013.
Mr. Islam was awarded JSPS postdoctoral fellowship (standard, 2 years) and joined the
laboratory of Professor Kazushige Yokota, Shimane University on the April
2014. Currently, he is doing research on the Pharmacogenomics of autism spectrum
disorder, schizophrenia and cervical cancer of Bangladeshi population. He has more than
66 peer-reviewed publications and submitted few more in the related peer-reviewed
journals.
iPROMISE International Seminar 2017
28-29th November 2017, UiTM Puncak Alam
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Keynote Lecture by Prof. Dr Mohammad Safiqul Islam
28thNOV
0930
Pharmacogenomics: The Way to Personalized Medicine
Drug treatment is customized in case of personalized medicine (PM) based on the genetic
profile of individual patients. The associations between variations on those genes that may
cause susceptibility to certain diseases or may cause abnormal reactions to certain drugs that
people normally take are the basis of PM. By looking at the peoples' genetic makeup one can
design therapies and choose drugs that can minimize toxicities and maximize benefits. One is
able to tailor therapies specifically for an individual person based upon one’s genetic makeup.
Even though the task might seem a lot of works requiring huge space but because of cutting-
edge genomic technologies it is possible to do large scale genetic project in a very small area.
Today the necessary instruments for genetic profile analysis are micro or miniaturized and the
process relies on commercially available chips encoded with up to half a million or so common
genetic variations. Scientists now have a complete picture of humans and therefore they are able
to scan millions of genes in the amount of times of just a few hours which in the past required
years- even tens of years in order to do the same thing. Pharmacogenetic techniques have been
successfully applied in treating (choosing personalized medicines) different diseases including
cancers, cardiac diseases, liver disease etc.
iPROMISE International Seminar 2017
28-29th November 2017, UiTM Puncak Alam
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Invited Speaker’s Abstract
28thNOV
1100
Exploring the Human Genome: Unlocking Life’s Code
Yu Choo Yee, Ang Geik Yong, Teh Lay Kek and Mod Zaki Salleh
Integrative Pharmacogenomics Institute (iPROMISE), Universiti Teknologi MARA, Puncak
Alam, Selangor, Malaysia.
The ambitious idea to map all the genes of the human genome dated back to the 1990s and the
through the Human Genome Project, the first human genetic map, which took 13 years to
complete and cost 2.7 billion dollars, was released in 2003. The availability of the first human
genetic map has greatly revolutionised genomic studies and accelerated the research into human
health, gene expression, human genetic variation, pharmacogenomics and genome-wide
association studies. Driven by the advancement of next-generation sequencing or massively
parallel sequencing, sequencing of human genome is poised to be become a common practice in
genomic medicine and precision medicine. The enormous interest and funding to unravel the
association between genetic changes and with various health conditions have lead a greater
understanding of human health, disease and development but the current knowledge may only
represent the tip of the iceberg. In this presentation, the road map of Human Genome Project
and the current approaches as well as the challenges of utilizing human genomic information in
clinical applications will be discussed.
iPROMISE International Seminar 2017
28-29th November 2017, UiTM Puncak Alam
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Invited Speaker’s Abstract
28thNOV
1130
Sports Genomics: Translating Potential into Performance
Ang Geik Yong1,2, Yu Choo Yee1, Teh Lay Kek1 and Mohd Zaki Salleh1
1Integrative Pharmacogenomics Institute (iPROMISE), Universiti Teknologi MARA, Puncak
Alam, Selangor, Malaysia. 2Faculty of Sports Science and Recreation, Universiti Teknologi MARA, Shah Alam,
Selangor, Malaysia.
Sports genomic is a relatively new field that focuses on the genomic architecture of elite
athletes. It seeks to unravel the genetic endowments that would explain, in part, why some
individuals reach the upper end of the performance continuum while others do not. Studies have
shown that inter-individual variability in physical performance characteristics is associated with
natural genetic variations that leads to improved athletic capability. The knowledge on genetic
factors that are associated with human physical performance to date are derived mainly from
Caucasian populations and as such, there is a huge research gap in the field of sports genomics
in Malaysia. Research into the genetic architecture of our local athletes is long overdue as
exemplified by the recent replication of association between physical performance and the first
performance enhancing polymorphism that was discovered almost two decades ago in 1998 in
Malaysian university athletes. The human physical performance is a multigenic trait and hence,
variation in athletic performance may be attributed to a polygenic profile instead of single
genetic variant. Therefore, there is urgent need to determine the allelic and genotype
frequencies of multiple performance enhancing polymorphisms in elite Malaysian endurance
and power/speed athletes so that the association between the genetic variants and physical
performance in these athletes from the competing ends of the strength-endurance continuum can
be unraveled. The resulting database will then serve as the basis to establish endurance and
power-based polygenic profiles of our local athletes.
iPROMISE International Seminar 2017
28-29th November 2017, UiTM Puncak Alam
5
Invited Speaker’s Abstract
28thNOV
1200
Basic Applied Bioinformatics
Vinothini Subramaniam, Teh Lay Kek and Mod Zaki Salleh
Integrative Pharmacogenomics Institute (iPROMISE), Universiti Teknologi MARA, Puncak
Alam, Selangor, Malaysia
Bioinformatics is a field of study that uses computation to extract knowledge from biological
data. It includes the collection, storage, retrieval, manipulation and modelling of data for
analysis, visualization or prediction through the development of algorithms and software.
Applied bioinformatics is the pertinent information such as downloading molecular sequences
(nucleotide and protein) from databases, BLAST analyses, primer designing and its quality
checking, multiple sequence alignment (global and local using freely available software),
phylogenetic tree construction (using UPGMA, NJ, MP, ME, FM algorithm and MEGA7 suite),
prediction of protein structures and genome annotation, RNASeq data analyses and
identification of differentially expressed genes and similar advanced bioinformatics analyses.
Computerized analysis has a role both as support for wet-lab projects and as a means of
extracting knowledge from already available datasets. The fast-growing amount of data makes
it necessary to be able to automate analysis and make analysis on a very large scale.
iPROMISE International Seminar 2017
28-29th November 2017, UiTM Puncak Alam
6
Invited Speaker’s Abstract
28thNOV
1230
Epigenetics: Food, Lifestyle & Environment = YOU
W.F.Lim, C.Y.Yu, G.Y.Ang, L.K.Teh, M.Z.Salleh
Integrative Pharmacogenomics Institute (iPROMISE), Universiti Teknologi MARA, Puncak
Alam Campus, Selangor, MALAYSIA
Have you ever wonder why you are the unique YOU? The answer lies with the power of
epigenetics. Epigenetics occurs beyond the ‘conventional genetics’, which is characterized by
the alternation of gene expression without the influence by an individual’s DNA sequence.
Epigenetic events (e.g. DNA methylation, histone modification and nucleosome positioning)
switch genes on and off and decide which proteins to be transcribed. These epigenetic marks
can be passed down from parents to offspring. Food that you eat, lifestyle that you practice and
the environment that you are exposed to, can influence your epigenetic machinery which can
either result in very healthy or extremely unhealthy conditions that lead to disease. Obesity is a
disease condition where abnormal or excessive fat accumulation that may impair health.
Garcinia atroviridis is commonly found in Malaysia and is used as an anti-obesity agent. We
extend our interest to investigate the intergenerational epigenetic response conferred by
Garcinia atroviridis extract in rat adipocytes. Besides obesity, heroin addiction is a growing
concern that affects many countries. Drug exposure has been associated to persistent changes in
gene expression and can be inherited to unexposed offspring. Our preliminary study
demonstrated that rats over three generations sustained anxiety and aggressive behaviour,
attributed by paternal heroin exposure. We are now investigating the heroin-induced epigenetic
mechanism of this observed effect. In short, any epigenetic marks will make you, YOU! Hence,
to realize the promise of precision medicine, both genetics and epigenetics diagnostic testing are
required.
iPROMISE International Seminar 2017
28-29th November 2017, UiTM Puncak Alam
7
Invited Speaker’s Abstract
28thNOV
1400
Microwave and 5-Fluorouracil Ethosomes Combination for Enhanced Skin
Drug Retention
Nauman Rahim Khana,b,c, Tin Wui Wonga,b*
aNon-Destructive Biomedical and Pharmaceutical Research Centre, iPROMISE bParticle Design Research Group, Faculty of Pharmacy
Universiti Teknologi MARA, Puncak Alam, 42300, Selangor, Malaysia. cFaculty of Pharmacy, Gomal University, DIKhan, 29050, KPK, Pakistan.
*Corresponding author. Non-Destructive Biomedical and Pharmaceutical Research Centre,
iPROMISE, Universiti Teknologi MARA, Puncak Alam, 42300, Selangor, Malaysia.
Tel.: +60 3 32584691
E-mail addresses: [email protected], [email protected] (T.W. Wong),
[email protected] (N.R. Khan)
Skin drug retention is detrimental to treat deep skin pathologies locally with minimal systemic
drug absorption. Ethosomes are well-known elastic vesicular carriers which increase the skin
drug retention by fusing with the skin. Combined use of microwave pre-treatment of skin and
ethosomes may seal the skin drug permeation pathways and hence lead to increased drug
deposition in the skin. 5-fluorouracil ethosomes with varying quantities of ethanol were
developed and subjected to size, surface charge, morphology, drug content, drug release and in
vitro drug permeation tests. The ethosomes were then investigated for in vitro skin drug and/or
ethosomes retention and permeation in combination with skin pre-treatment with microwave.
The mechanism of enhanced skin drug retention was investigated with fourier transform
infrared and raman spectroscopy, thermal and electron microscopic techniques. Low ethanolic
ethosomes promoted skin drug retention as they were larger in size, had high negative charge
and were less able to fluidize epidermal intercellular lipids and defluidize hydrophilic skin
domains. The skin pre-treatment with microwave at 2450 MHz frequency for 2.5 min further
promoted skin drug and/or ethosomes retention and reduced the skin drug permeation compared
to 1.15 min and 5 min treatment. The mechanistic investigations revealed that microwave and
ethosomes synergized to promote skin drug retention by macromolecular swelling and
expansion of specific deep dermal proteins via C=O moiety which translated to narrowing of
deep skin intercellular spaces, increased drug retention and reduced drug permeation.
iPROMISE International Seminar 2017
28-29th November 2017, UiTM Puncak Alam
8
Invited Speaker’s Abstract
28thNOV
1430
Profiling of Skin Drug Delivery of Chitosan-Surfactant Nanoparticles
Asif Nawaza,b,c*, Tin WuiWonga,b*
aNon-Destructive Biomedical and Pharmaceutical Research Centre, iPROMISE
bParticle Design Research Group, Faculty of Pharmacy Universiti Teknologi MARA,
42300, Puncak Alam, Selangor, Malaysia. cAhmad Medical Institute Peshawar, Pakistan.
Email Address:
[email protected]; [email protected]
This study investigated the transdermal drug delivery mechanisms of chitosan-surfactant
nanoparticles in the skin modification and transport. Low molecular weight chitosan
nanoparticles, were prepared by nanospray-drying technique with tween 20 and span 20 as
additives. The transdermal drug delivery profiles of nanoparticles across the rat skins were
examined. The average size and zeta potential of the chitosan-surfactant nanoparticles were
85.0 ± 3.5 nm and 42.7 ± 2.7 mV, respectively. Chitosan along with tween and span of
nanoparticles were required to succeed the transdermal delivery. The ninhydrin assay of
nanoparticulate chitosan revealed that the chitosan-surfactant nanoparticles permeated through
the skin and a fraction of the permeated nanoparticles were retained in the skin. The population
of chitosan-surfactant nanoparticles was found higher in dermis than epidermis according to
FTIR Imaging results. The nanoparticles transport was facilitated through constituent
nanoparticles fluidizing both protein/lipid domains of epidermis and dermis (O-H, N-H, C-H,
C-N), largely affecting the palmitic acid and keratin domains. Future study will further probe
into the effects of nanoparticles on tight junction of human skin and its changes on transdermal
drug transport.
iPROMISE International Seminar 2017
28-29th November 2017, UiTM Puncak Alam
9
Invited Speaker’s Abstract
28thNOV
1500
Treatment of Pulmonary Tuberculosis Using Rifampicin-Loaded Double
Receptor-Targeting
Kifayat Ullah Shah1,3*, Wong Tin Wui1,2*
1Non-Destructive Biomedical and Pharmaceutical Research Centre, iPROMISE 2Particle Design Research Group, Faculty of Pharmacy
Universiti Teknologi MARA, 42300, Puncak Alam, Selangor, Malaysia 3Department of Pharmaceutics, Faculty of Pharmacy, Gomal University, 29050, D.I.Khan,
Khyber Pakhtunkhwa, Pakistan
*[email protected], [email protected]
Most of the current anti-tubercular drugs fail to penetrate into alveolar macrophages that harbor
mycobacterium tuberculosis. In conjunction with tubercle bacilli lurking in macrophages, this
then translates to reduced therapeutic effectiveness and need for higher drug doses and hence
toxicity. The study aims to develop o/w nanoemulsions that can aerosolize the drug in
appropriate particle size and concentration to ensure optimal deposition and dose in the desired
region of the lungs. Chitosan-folate conjugate-decorated third generation rifampicin-oleic acid
nanoemulsion was designed with chitosan and folate acting as the homing device for
macrophages with mannose and folate receptors at their cell surfaces. The nanoemulsion was
prepared by conjugate synthesis and spontaneous emulsification techniques. It was then
subjected to physicochemical, drug release, aerosolization, inhalation, cell culture and
pharmacokinetics analysis. The nanoemulsion had average droplet sizes of 40-60 nm, with
narrow polydispersity indices. It exhibited desirable pH, surface tension, refractive index,
density, and viscosity attributes for pulmonary rifampicin administration. The nanoemulsion
demonstrated more than 95 % aerosol output and inhalation efficiency greater than 75 %. The
nanoemulsion was found to be safe with negligible cytotoxicity effects. It exhibited high
macrophage cell internalization potential, reduced plasma drug concentration and high lung
drug content. Nanoemulsion has a great potential to deliver anti-tubercular drugs by
nebulization in the treatment of tuberculosis.
iPROMISE International Seminar 2017
28-29th November 2017, UiTM Puncak Alam
10
Invited Speaker’s Abstract
28thNOV
1530
Preparation and evaluation of alginate-C18 conjugate nanoparticles
embedded into coated calcium alginate beads for efficient oral insulin
delivery
MulhamAlfatama,a,b Lee Yong Lim,c Tin WuiWonga,b*
aNon-Destructive Biomedical and Pharmaceutical Research Centre, iPROMISE bParticle Design Research Group, Faculty of Pharmacy
UniversitiTeknologi MARA Selangor, PuncakAlam, 42300, Selangor, Malaysia. cPharmacy,Centre for Optimisation of Medicines, School of Allied Health, The University of
Western Australia, 35 Stirling Highway, Crawley WA 6009, Australia.
The rapid drug release from natural polymeric nanoparticles and the physical barriers including
mucus and mucosa have precluded the efficient penetration and absorption of therapeutics
particles. In this study, a complex of nanoparticles and beads were designed using alginate and
chitosan as matrix and coat materials respectively. Three types of nanoparticles namely simple
alginate, alginate-stearic acid and alginate-C18 conjugate were formulated by chemical
synthesis and nanospray drying technology. The nanoparticles were characterized regarding
their size, zeta potential, surface morphology, drug content, drug encapsulation efficiency, drug
release profile, matrix molecular characteristics, mucus penetration, HT-29 cell line cytotoxicity
and intracellular trafficking profiles. Alginate-C18 conjugate nanoparticles were selected as
insulin nanocarrier of interest due to their non-toxicity and favourable physicochemical
attributes which enhanced mucus penetration and intracellular trafficking, and minimal insulin
reabsorption tendency. These nanoparticles were loaded into a pre-optimized tripolyphosphate-
crosslinked chitosan-oleic acid conjugate-coated calcium alginate beads by vibratory nozzle
microencapsulation techniques. Incorporation of nanoparticles into coated beads successfully
reduced insulin release in the simulated gastric phase with the majority of insulin being
transported transmucosally in the form of nanoparticles. Nanoparticles and beads combination
system has found to be more effective in lowering blood glucose level and enhancing insulin
bioavailability than nanoparticles alone.
iPROMISE International Seminar 2017
28-29th November 2017, UiTM Puncak Alam
11
Invited Speaker’s Abstract
29thNOV
0900
Secret to Stay Young with “Murunggai”
M.S. Rofiee, L.K. Teh, M.Z.Salleh
Integrative Pharmacogenomics Institute (iPROMISE), Lelvel 7, FF3, Universiti Teknologi
MARA Kampus Selangor
Ageing in humans refers to a multidimensional process of mainly physical and psychological
change. Approximately 100,000 people worldwide die each day of age-related causes. Life
extension science, also known as anti-ageing medicine, is the study of slowing down the
processes of ageing to extend both the maximum and average lifespan. We therefore searched
for a herbal alternative for slowing down the process of ageing. From our study, the
supplementation of TGT-PRIMAAGE which is a combination of M. oleifera and Centella
asiatica leaves extract to the ageing rats showed improvement in memory and learning abilities.
Histopathological examinations on the ageing skin also showed significant protection in the
groups of ageing rats treated with TGT-PRIMAAGE. With the evidence and its efficacy, the
extract was formulated as preservative-free capsule for easy consumption and dose
standardization. This product can be used as a supplement to improve memory and learning
abilities. Besides that, this product may protect the skin from damage through the anti-glycation
process.
iPROMISE International Seminar 2017
28-29th November 2017, UiTM Puncak Alam
12
Invited Speaker’s Abstract
29thNOV
1100
Zebrafish as Human Disease Model
Muhammad Hisyam Jamari, Teh Lay Kek and Mod Zaki Salleh
Integrative Pharmacogenomics Institute (iPROMISE), Universiti Teknologi MARA, Puncak
Alam, Selangor, Malaysia
Zebrafish (Danio rerio) have become more important to biological research ever since it was
made popular by George Streisinger in the 1960s. The small freshwater fish, native to parts of
the Himalayas and South East Asia have many advantages over other vertebrate animal models.
Amazingly, zebrafish have similar genetic structure to human and they share about 70% of the
genes with human. It is a very efficient model - high fecundity and short life cycle allow
research to be carried out at a larger scale and shorter time. Various diseases can be studied
using zebrafish, ranging from acquired diseases to genetic diseases, as 84 per cent of genes
known to be associated with human disease have a zebrafish counterpart. Their external
reproduction allows genetic study, and the transparent embryo allows developmental research to
be carried out easier. It is thus very versatile and frugal - it is becoming more popular within the
scientific community and various methods have been established to study many human
diseases.
iPROMISE International Seminar 2017
28-29th November 2017, UiTM Puncak Alam
13
Postgraduate Student’s Abstract
28thNOV
1430 Multi-omics of Thermal Adaptation Strategies of Geobacillus
thermocatenulatus and Some of Hot Spring Thermophiles
Fakhruzzaman, M.N.N1, Teh, L.K1 Zaki, M.S1, Nazrina, S.C2
1Integrative Pharmacogenomics Institute (iPROMISE), Universiti Teknologi MARA Puncak
Alam, Selangor, Malaysia 2Faculty of Health Sciences, Universiti Teknologi MARA Puncak Alam, Selangor, Malaysia
Geobacillus thermocatenulatus is a facultative anaerobic thermophilic bacterium isolated from
Sungai Klah hot spring in Perak, Malaysia. A study was designed to elucidate the thermal
adaptation strategy adopted by this bacterium which allows it to strive at high temperature
which normally inhibits life. Here, we report the proteins associated in G. thermocatenulatus
thermoadaptation. To induce thermal stress, G. thermocatenulatus was grown at 60°C and 70°C
where the latter represent thermal stress condition. Upon induction of thermal stress, a biphasic
growth profile was observed from the bacterium growth curve. Next, SDS-PAGE analysis
disclosed substantial changes in the proteome profile. Protein identification by MS/MS revealed
that the bacterial proteome when grown at 60°C and 70°C contain 1490 and 895 proteins
respectively. In addition, 195 proteins were found to be only expressed during thermal stress.
Functional annotation of the identified proteins indicated that the bacterial undergone nearly
50% of changes in its proteome in compensation to thermal stress. Additionally, pathways that
greatly influenced by thermal stress in this bacterium were amino acid metabolism and transport
and energy production and conservation. As a conclusion, this study successful in elucidating
components that contributed to the thermal adaptation of G. thermocatenulatus were
successfully elucidated.
iPROMISE International Seminar 2017
28-29th November 2017, UiTM Puncak Alam
14
Postgraduate Student’s Abstract
28thNOV
1445
Metabolomics of Colorectal Cancer in Malaysia
N.A.A. Hashim1, S. Ab-Rahim1, W.Z.W. Ngah2, S. Nathan3, M. Mazlan1, L. K. Teh4,5, M. Z.
Salleh4,5
1Faculty of Medicine, Universiti Teknologi MARA, Jalan Hospital, 47000 Sungai Buloh
Selangor 2Faculty of Medicine Universiti Kebangsaaan Malaysia, 56000 Cheras Kuala Lumpur
3Faculty of Science and Technology Universiti Kebangsaaan Malaysia, 43600 Bangi Selangor 4Integrative Pharmacogenomics Institute (iPROMISE), Universiti Teknologi MARA (UiTM)
Puncak Alam, Selangor 5Faculty Applied Science, Universiti Teknologi MARA (UiTM) Shah Alam, Selangor
Diagnosis of colorectal cancer (CRC) involves invasive techniques such as colonoscopy and
histopathology. Other screening tests are neither not accurate nor specific for CRC. This
contributes to the late diagnosis of CRC. Hence, a more accurate, specific and non-invasive
method for diagnosis is required for better prognosis. In this study, we aim to identify serum
biomarkers for detecting CRC using metabolomics. Serum from 50 healthy controls and 50
colorectal cancer patients were collected at Hospital UKM. The samples were deproteinized
with acetonitrile and then analyzed using liquid chromatography-quadrupole time-of-flight
mass spectrometry (LC-QTOFMS, Agilent USA). The data were analyzed using Mass Profiler
Professional (Agilent, USA) software. A total of 127 metabolites were obtained in the serum.
Among these 127 known metabolites, 12 metabolites were significant after recursion analysis.
Partial least squares discriminate analysis (PLS-DA) of metabolic profile data showed robust
discrimination between healthy controls and CRC patients. Based on area under the curve,
sensitivity and specificity values for lysophosphatidylethanolamine (LysoPE 22:6),
lysophosphatidylcholine (LysoPC 16:1), glycocholic acid, xanthine, malic acid and
hypoxanthine for detecting CRC were above 70%. The alterations in these metabolites reveal
perturbations of purine metabolism associated with increase growth and proliferation of CRC
cells. These results suggest that serum metabolomics profiling has great potential in identifying
biomarkers for CRC and helping to understand its underlying mechanisms.
iPROMISE International Seminar 2017
28-29th November 2017, UiTM Puncak Alam
15
Postgraduate Student’s Abstract
28thNOV
1500
Effect of Lansium domesticum Leaves Ethanolic Extract on Anxiety-Like
Behaviour in Zebrafish
Noor Fahimah Saari1, Salfarina Ramli1, Teh Lay Kek1.2, Mohd Zaki Salleh2, Richard Johari
James1,2
¹ Faculty of Pharmacy, Universiti Teknologi MARA, Puncak Alam Campus, Bandar Puncak
Alam, Selangor Darul Ehsan, Malaysia 2Integrative Pharmacogenomics Institute (iPROMISE), UiTM Selangor, Puncak Alam Campus,
42300 Bandar Puncak Alam, Selangor
New plant natural compounds or plant extracts are frequently under investigation to explore
alternative to conventional anxiolytic therapies. Exposure to novelty evokes robust anxiety
responses in zebrafish. Therefore, zebrafish has emerged as a useful animal model to study
anxiety-like behaviour. In this study, adult zebrafish was exposed to L.domesticum ethanol
leaves extract (20 mg/L) before being introduced to the novel tank. The behaviour of treated
zebrafish in novel tank, untreated zebrafish in novel tank and home tank were recorded for 6
minutes and the video outputs were analysed using Any-maze software V.5.2. From the results,
the treated zebrafish showed significant (p<0.05) decrease in the behaviour parameters which
include time spent in top, number of entries to the top and total distance travelled compared to
zebrafish in home tank which indicated elevated state of anxiety. However, no significant
behavioural changes were observed between treated and untreated zebrafish in novel tank which
indicated that L. domesticum leaves ethanolic extract did not affect the anxiety-like behaviour in
the novel tank experiment.
iPROMISE International Seminar 2017
28-29th November 2017, UiTM Puncak Alam
16
Postgraduate Student’s Abstract
28thNOV
1515
Quantification of Cortisol Using LC/MS/MS in Relation to Stress among
University Students
A. Hazirah1,2, M. S. Rofiee2, A.G. Rohana2,3, L. K. Teh1,2, M. Z. Salleh1,2R. J. James1,2
1Faculty of Pharmacy, Universiti Teknologi MARA Puncak Alam Campus, Selangor, Malaysia;
2Integrative Pharmacogenomics Institute (iPROMISE), Universiti Teknologi MARA Puncak
Alam Campus, Selangor, Malaysia; 3Faculty of Medicine, Universiti Teknologi MARA Sungai
Buloh Campus, Selangor, Malaysia
Pursuing higher education at the university is perhaps a stressful life event for some student as
they need to adapt with various environmental and psychosocial changes. Previously, cortisol
has been measured in several types of biological sample for evaluation of physiological and
psychological stresses, anxiety and depression. The aim of this study is to develop a method for
quantification of cortisol using liquid chromatography mass spectrometry (LC-MS/MS) and
correlate with the level of depression, anxiety and stress among undergraduate students. A total
of 24 undergraduate students were administered with Depression Anxiety Stress Scale (DASS)
and serum sample were collected during the beginning of semester, middle of semester and
final examination week. The cortisol was quantitated using a fast, selective and sensitive LC-
MS/MS. The method was developed and validated according to European Medicine Agency
(EMA) guidelines and was linear from 7.8 to 500 ng/mL for cortisol (r2 = 0.986). The
precision, accuracy, and recovery of the method for cortisol were ranged from 1.98-10.46%,
90.68-91.94%, 90.38-93.88%. The cortisol level at the beginning of the semester was
significantly lower than the cortisol level at middle of semester. Furthermore, the concentration
of cortisol in serum was significantly correlated with stress level. Therefore, the developed
method has met the quality standards of EMA, can be utilized for quantitation of cortisol in
serum and correlate with the DASS data to measure and monitor stress condition of
undergraduate students.
iPROMISE International Seminar 2017
28-29th November 2017, UiTM Puncak Alam
17
Postgraduate Student’s Abstract
28thNOV
1530
Understanding the Genetic Risk of Childhood Leukemia among the Orang
Asli and Malay in Malaysia: Mining the Whole Genome Sequence Database
R.H. Razalia, N.A. Khoruddina, H. Jamaria, G.Y. Anga,b, C. Y. Yua,b, R. J. Jamesa, K.H. Tehc, H.
Ibrahimc, L.K Teha and M. Z. Salleha
aIntegrative Pharmacogenomics Institute (iPROMISE), Universiti Teknologi MARA, Selangor
(UiTM), Malaysia bFaculty of Sports Science and Recreation, Universiti Teknologi MARA (UiTM), Malaysia
cDepartment of Pediatric, Pediatric Institute, Hospital Kuala Lumpur.
Leukemia is the seventh most common childhood malignancy in Malaysia. It is of great interest
to determine the genetic factors that cause leukemia in order to strategise for prevention and
treatment modalities. We aimed to determine the genetic variability that increase ALL
susceptibility of the Orang Asli and the Malays using existing genome database. Genomic DNA
from the Orang Asli were isolated from blood and the whole genome sequencing analysis were
performed. Genomes were assembled, aligned and variants were called using GATK Best
Practise workflow. Leukemic-associated variants were identified and analysed for the Orang
Asli and Malays. A total of 98 Orang Asli and 100 Malays were recruited in this study. Of the
126 codon changes identified, the number of variants predicted to be deleterious among the
Orang Asli and the Malays were 24 and 2, respectively. The SNPs with the highest allele
frequency associated with ALL risk in the Orang Asli and the Malays was XRCC1rs25487-G
with 72.44% and 66%, respectively. This study is believed to be the first to report on the higher
predicted genetic risks of susceptibility against leukemia among the Orang Asli compared to the
Malays.
iPROMISE International Seminar 2017
28-29th November 2017, UiTM Puncak Alam
18
Postgraduate Student’s Abstract
28thNOV
1545
Differential Protein Expression in Bone Remodelling Induced by Denture
Wearing – Preliminary Results
U. H. Anis1, R. Ahmad2,3, L. K. Teh3, M. Z. Salleh3
1Faculty of Dentistry, Universiti Teknologi MARA Sungai Buloh Campus, Sungai Buloh,
Selangor, Malaysia;
2Unit of Prosthodontics, Faculty of Dentistry, Universiti Teknologi MARA Sungai Buloh
Campus, Sungai Buloh, Selangor, Malaysia; 3Integrative Pharmacogenomics Institute
(iPROMISE), Bandar Puncak Alam, Selangor, Malaysia
The continuous and intermittent mechanical pressure exerted by complete and partial dentures
on the soft tissue mucosa of edentulous patients induce inflammation to the supporting tissues
underlying the dentures. This lead to alveolar bone resorption and triggered secretion of various
proteins and enzymes into saliva. This study aims to identify the change in protein expression
associated with bone remodelling induced by denture wearing. These differentially expressed
proteins can potentially serve as protein biomarkers for bone remodelling in denture wearing
patients. The protocol of the study was approved by Human Research Ethics Committee, UiTM.
Patients attending treatment at Dental Clinic, UiTM were recruited after informed consent form
was obtained. Unstimulated whole saliva was collected twice from patients of complete and
partial dentures, once before the issuance of denture (T0) and another after 30 days post denture
insertion (T1). Salivary proteins were resolved using two-dimensional gel electrophoresis (2DE)
over a pH range of 3-10, and the resulting proteome profiles were compared. Differentially
expressed proteins were then identified by Q-TOF LCMS. There were differences in protein
profile between T0 and T1 of each patient when the saliva sample was subjected to 2DE. These
data are significant in explaining the mechanism involved in bone resorption phenomenon
associated with denture wearing. The discovery of protein biomarker in denture wearers with
alveolar bone resorption allows the identification of high risk patients in order to avoid severe
bone resorption.
iPROMISE International Seminar 2017
28-29th November 2017, UiTM Puncak Alam
19
Postgraduate Student’s Abstract
28thNOV
1600
Vision Threatening Amoeba: Morphological and Molecular Based Evidence
Isolated from Contact Lens Users, Malaysia
Rosnani Hanim Mohd Hussain1, Mohamed Kamel Abd Ghani2, Anisah Nordin3, M. Z. Salleh1,4
, Tengku Shahrul Anuar1,4
1Centre of Medical Laboratory Technology, Faculty of Health Sciences, Universiti Teknologi
MARA, Puncak Alam Campus, 42300 Selangor, Malaysia 2Biomedical Science Programme, Faculty of Health Sciences, Universiti Kebangsaan Malaysia,
Jalan Raja Muda Abdul Aziz, 50300 Kuala Lumpur, Malaysia 3Department of Parasitology and Medical Entomology, Universiti Kebangsaan Malaysia
Medical Centre, Jalan Yaacob Latif, 56000 Kuala Lumpur, Malaysia 4Integrative Pharmacogenomics Institute, Universiti Teknologi MARA, Puncak Alam Campus,
42300 Selangor, Malaysia
Acanthamoeba keratitis is a serious eyes infection among contact lens wearers and it is caused
by an opportunistic free-living amoeba known as Acanthamoeba spp. Trophozoite and cyst of
this parasite can be found ubiquitously in the environment. Therefore, the aim of this study was
to characterize the morphology and genotypes the species of Acanthamoeba from contact lens
users in Malaysia. Two clinical samples in the form of cyst culture were obtained from private
hospital. Sub-cultured was done on non-nutrient agar seeded with heat-killed Escherichia coli
and incubated at 30°C (±2oC) for 10 days. Morphological identification was performed using
methylene blue stain based on shape and size of the endocyst and ectocyst. Observation was
made under an inverted microscope (x40). Genomic DNA samples were extracted and PCR
assay was conducted for amplification of the Acanthamoeba-specific amplimer (ASA.S1)
region of the 18S ribosomal RNA gene. The phylogenetic analysis was carried out using Unipro
UGENE software. It was observed that both clinical isolates have cyst sizes 18 µm with
wrinkled exocyst and stellate endocyst. This morphological characterization demonstrated that
the isolates belonged to Group II (Polyphagids). Product of approximately 464-bp was obtained
using JDP1 and JDP2 primers. Phylogenetic analysis revealed that it belongs to genotype T4. In
conclusion, the presence of Acanthamoeba Group II with genotype T4 resembled with previous
studies that shown the major Acanthamoeba keratitis cases are associated with this genotype.
Thus, Acanthamoeba infection is no more a rarity and can be a public health issue among the
Malaysian community.
iPROMISE International Seminar 2017
28-29th November 2017, UiTM Puncak Alam
20
Postgraduate Student’s Abstract
28thNOV
1615
In silico Molecular Docking Studies on Chalcone and Flavones Derivatives as
Multi-Subtypes Inhibitors of Phosphodiesterase
Muhd Hanis Md Idris, Manikandan Selvaraj, Teh Lay Kek, Mohd Zaki Salleh
Integrative Pharmacogenomics Institute (iPROMISE), Universiti Teknologi MARA Selangor
Branch, Puncak Alam Campus, 42300 Bandar Puncak Alam, Selangor
Phosphodiesterase (PDE) is known to promote inflammation by degrading cyclic adenosine
monophosphate (cAMP) which is intracellular secondary messenger that help in maintaining
immune homeostasis. Therefore, selective inhibition of PDE elevates cAMP and consequently
downregulate inflammation. The structural motifs of chalcones and flavones with varied
substitutions had been studied for the anti-inflammatory and analgesic activity. However, there
is lack of study on chalcones and flavones as PDE inhibitors so far. Thus, this study was to
investigate the phosphodiesterase activity of chalcones and flavones using molecular docking.
A series of chalcone and flavone analogs were synthesized and virtually screened using
molecular docking against multi-subtype of phosphodiesterase (PDE4B, PDE4D, PDE3B,
PDE7A). The results showed that seven compounds exhibited good binding activity against all
subtypes of PDE ranging between -10.656 kcal/mol – -5.206 kcal/mol. Molecular docking
experiments indicated that π-π interaction, hydrogen-bonding, and hydrophobic interactions
were all contributed to interactions between compounds and targets. Further, these compounds
were predicted to possess good pharmacokinetics properties with less side effects. Based on
binding energies, these molecular docking analyses could lead to the further development of
potent phosphodiesterase inhibitors for the treatment of inflammatory disorders.
iPROMISE International Seminar 2017
28-29th November 2017, UiTM Puncak Alam
21
Postgraduate Student’s Abstract
28thNOV
1600
Chemical Diversity Analysis in Indian Turmeric (Curcuma longa L.) by
HPTLC Method
Kamran Ashraf 1,2,3
1 Faculty of Pharmacy, Universiti Teknologi MARA, Puncak Alam Campus, Bandar
Puncak Alam, Selangor Darul Ehsan, Malaysia 2 Atta-ur-Rahman Institute for Natural Products Discovery (AuRIns), Universiti Teknologi
MARA, Puncak Alam Campus, Bandar Puncak Alam, Selangor Darul Ehsan, Malaysia 3 Bioactive Natural Product Laboratory, Department of Pharmacognosy and Phytochemistry,
Faculty of Pharmacy, Jamia Hamdard Univ, New Delhi-62, India
Curcuma longa is a valued medicinal plant belonging to the family Zingiberaceae which
comprises more than 80 species of rhizomatous perennial herbs and has extensive occurrence in
the tropics of Asia Africa and Australia. To access chemical diversity analysis in accessions of
Curcuma longa L. of Indian subcontinent. Phytochemical constituent curcumin of all turmeric
accessions was evaluated by High Performace Thin Layer Chromatography (HPTLC) method.
Results obtained from chemo profiling showed that C. longa undergone chemical variations
among samples collected across indian subcontinent. Chemical variation occurred in turmeric
may be due to wide range of ecological conditions within distribution area of its population in
India. The outcome would provide an important input into determining efficient management
strategies for the cultivation of C. longa and its improvement program.
iPROMISE International Seminar 2017
28-29th November 2017, UiTM Puncak Alam
22
Postgraduate Student’s Abstract
28thNOV
1615
Development of Resistant Corn Starch for Use as an Oral Colon-Specific
Nanoparticulate Drug Carrier
Norul Nazilah Ab’laha,b,d, Nagarjun Konduru Venkatac, Tin Wui Wonga,b*
aNon-Destructive Biomedical and Pharmaceutical Research Centre, iPROMISE bParticle Design Research Group, Faculty of Pharmacy
Universiti Teknologi MARA, Puncak Alam, 42300, Selangor, Malaysia cDepartment of Environmental Health, Harvard T.H. Chan School of Public Health, Harvard
University, 665, Huntington Avenue, Boston, Massachusetts, 02115, USA dCentre of Foundation Studies, Universiti Teknologi MARA, Dengkil, 43800, Selangor,
Malaysia
*[email protected], [email protected]
Amylose and amylopectin are the main constituent in starch. Debranching of amylopectin
converts it into amylose thereby producing resistant starch which is known to be less digestible
by amylase. This study examines acid hydrolysis and heat-moisture treatment methods in
designing of resistant starch as a drug carrier from native corn starch. The starch was subjected
to treatment using 0.2M hydrochloric acid buffer, pH 2.2 at 37o ± 0.2oC or at 90oC, 100oC and
110oC for 2h and 16h heating in the presence of 25% moisture content. Both native and
processed starch were subjected to Fourier transform infrared spectroscopy, X ray
diffractometry, differential scanning calorimetry and molecular weight analysis. They were
nanospray-dried with 5-fluorouracil as a drug of interest for colon cancer treatment. These
nanoparticles were subjected to size, zeta potential, morphology, drug content and in-vitro drug
release analysis. Heat-moisture treatment of native corn starch at 100oC for 2h enabled the
formation of resistant starch through amylopectin debranching and molecular weight reduction
thereby enhancing hydrogen bonding between the starch molecules at the amorphous phase and
gelatinization capacity. With reduced branching, it represents an ideal precursor for targeting
ligand conjugation in design of oral colon-specific nanoparticulate drug carrier.
iPROMISE International Seminar 2017
28-29th November 2017, UiTM Puncak Alam
23
Postgraduate Student’s Abstract
28thNOV
1630
Design of Low Molecular Weight Pectin and Its Nanoparticles through
Combination Treatment of Pectin by Microwave and Inorganic Salts
Badrul Hisyam Zainudin a, b, c, d, Wong Tin Wui a, b, *, Halimaton Hamdan d
a Non-Destructive Biomedical and Pharmaceutical Research Centre, iPROMISE, Universiti
Teknologi MARA, Puncak Alam, 42300, Selangor, Malaysia b Particle Design Research Group, Faculty of Pharmacy, Universiti Teknologi MARA, Puncak
Alam, 42300, Selangor, Malaysia14 c Malaysian Cocoa Board, Cocoa Innovative and Technology Centre, Lot 12610, Kawasan
Perindustrian Nilai, Nilai, 71800, Negeri Sembilan, Malaysia d Razak School of Engineering and Advanced Technology, Universiti Teknologi Malaysia,
54100, Kuala Lumpur, Malaysia
* Corresponding author: Non-Destructive Biomedical and Pharmaceutical Research Centre,
iPROMISE, Universiti Teknologi MARA, Puncak Alam, 42300, Selangor, Malaysia
E-mail address: [email protected], [email protected] (T.W. Wong)
This study assessed the molecular weight and degree of esterification profiles of pectin treated
by microwave (900 W) for different irradiation durations in combination with monovalent
(sodium chloride) or divalent (calcium acetate) inorganic salt as the promoter of superheating at
liquid state. The pectin molecular weight, degree of esterification, viscosity, particle size, zeta
potential and elemental content were determined. The pectin was subjected to nanospray drying
with the size, zeta potential and morphology of the formed nanoparticles examined. The use of
calcium acetate brought about the formation of pectin with lower molecular weight and degree
of esterification, but higher solution viscosity than that of sodium chloride. Such pectin had its
molecules crosslinked by soluble calcium at COO- moiety in liquid phase. It experienced a
higher heat transfer through salt bridges and chain breakdown propensity. The formed
nanoparticles had a mean size smaller than 600 nm and were envisaged suitable for use as
nanocarrier of cancer therapeutics with respect to permeation and retention attributes of tumour
vasculature. The combination of microwave with multivalent inorganic salt approach can be
used to convert the pectin into matrix material of nanoparticles.
iPROMISE International Seminar 2017
28-29th November 2017, UiTM Puncak Alam
24
Postgraduate Student’s Abstract
29thNOV
0930
Whole Genome Sequence Analysis of a Clinical Multi Drugs Resistant
Mycobacterium tuberculosis (MDR-TB)
N. Z. Abidin1,2, F.Z.M Yusof1,2, N.M. Noordin6, C.Y. Yu1, G.Y. Ang1,3, A.I. Ismail4, L.K.
Teh1,5, M.Z. Salleh1,5
1 Integrative Pharmacogenomic Institute (iPROMISE), UiTM Selangor, Puncak Alam Campus
2 Faculty of Applied Science, Universiti Teknologi MARA (UiTM) Selangor, Shah Alam Campus 3Faculty of Sport Sciences and Recreation, Universiti Teknologi MARA (UiTM) Selangor, Shah
Alam Campus 4 Faculty of Medicine, Universiti Teknologi MARA (UiTM) Selangor, Sungai Buloh
Campus 5Faculty of Pharmacy, Universiti Teknologi MARA (UiTM) Selangor, Puncak Alam
Campus 6National Public Health Laboratory, Ministry of Health, Malaysia
Tuberculosis (TB) is an infection caused by Mycobacterium tuberculosis (MTB). In 2016, cases
of rifampicin-resistant Tuberculosis (RR-TB) which had resulted in death had been reported by
WHO, at the same time cases of extensively drug-resistant TB (XDR-TB) were reported at least
one in all the countries including Malaysia. We believed that changes in the genomic structure
of the pathogen are responsible for the resistance pattern observed and are useful diagnostic
markers. Together with National Public Health Laboratory (Makmal Kesihatan Awam
Malaysia), the deposit of MTB strains isolated from patients who were diagnosed with MDR-
TB will be sequenced. We present here one of the MDR-TB strain (PR12) which were
successfully sequenced and annotated. The genomic DNA of the MDR-TB strain was extracted
and sequenced with the next-generation sequencing (NGS) approach using the Miseq
(Illumina). The output sequences were processed and analysed with the bioinformatics tools.
The single nucleotide polymorphism (SNPs) sites identified within the Mycobacterium
tuberculosis PR12 strain include the embB (Met306Val), katG (Ser315Thr), pncA (Leu85Pro),
rpoB (Ser450Leu) and rpsL (Lys43Arg). The variation on these genes were predicted to cause
the pathogen to be resistant towards ethambutol, isoniazid, rifampicin, pyrazinamide, and
streptomycin, respectively. Using the whole genome sequence analysis, the variations detected
can help to determine the resistance pattern of MTB and the information is useful for the
clinician to tailor made the correct antimycobacterial agent for the patients. It is also used to
track the evolution of the resistance patterns in order to control occurrence of TB and to identify
potential new therapeutic targets.
iPROMISE International Seminar 2017
28-29th November 2017, UiTM Puncak Alam
25
Postgraduate Student’s Abstract
29thNOV
0945
Genomic Analysis of Breast Cancer Risk for the Orang Asli and Malays
N.A. Khoruddin1,2, F.Z.M. Yusof1,2, Y.C. Yee1, A.G. Yong1,3, L.K. Teh1,4, M.Z. Salleh1,4
1Integrative Pharmacogenomics Institute (iPROMISE), Universiti Teknologi MARA(UiTM)
Selangor, Puncak Alam Campus 2 Faculty of Applied Sciences, Universiti Teknologi MARA(UiTM) Selangor, Shah Alam
Campus 3Faculty of Sports Science and Recreation, Universiti Teknologi MARA (UiTM), Shah Alam
Campus 4Faculty of Pharmacy, Universiti Teknologi MARA (UiTM) Selangor, Puncak Alam Campus
A comprehensive whole genome study to identify single nucleotide polymorphisms (SNPs)
that cause breast cancer is of great interest to implement precision medicine and develop
therapies that improve lives. Here, we report the whole genomes analysis using computational
approaches on unrelated 98 Orang Asli and 96 Malays individuals to search and identify SNPs
which confer risks of breast cancer. The SNPs were retrieved from dbSNP and predicted as
cancer driver variants based on the functional impact scores by CHASM and VEST tools that
were built in the CRAVAT software. Four candidates SNPs (rs33927012, rs28997576,
rs3518855, rs144848) were identified as cancer driver variants that were associated with the
breast cancer. Interestingly, all of the variants are located on SDHB, BARD1, FGFR4 and
BRCA2 genes which are involved in the breast cancer pathway. All of the SNPs are believed to
play important roles in activating the oncogenic genes in breast cancer. We had successfully
developed bioinformatics pipelines useful for prediction of functional impacts of SNPs which
are cancer driver variants that increase breast cancer risks. These markers would need to be
validated before being used as diagnostic tools in detecting breast cancer risk in the population.
iPROMISE International Seminar 2017
28-29th November 2017, UiTM Puncak Alam
26
Postgraduate Student’s Abstract
29thNOV
1000
Comparative Genomic Analysis of Mycobacterium tuberculosis in
Identification of Multidrug Resistance Genes
S. Suhaimi1,2, F. Z. Yusuf1,2, S. S. Noor3, L. K. Teh1,2, M. Z. Salleh1,2
1Integrative Pharmacogenomics Institute (iPROMISE), Universiti Teknologi MARA (UiTM)
Puncak Alam, Selangor 2Faculty Applied Science, Universiti Teknologi MARA (UiTM) Shah Alam, Selangor
3School of Medical Science, Universiti Sains Malaysia (USM), Kelantan
The current global challenge in the treatment of tuberculosis (TB) is the emergence of
multidrug resistant MTB (MDR-MTB) especially in most of the developing countries across the
world. Known causes for the emergence of MDR-TB include improper treatment, inadequate
drug use, and poor patient supervision. However, it is interesting to determine the genome
structure of the MTB and the existing resistance genes that they harbour. In this study, we
examined the complete genome dataset of 138 Mycobacterium tuberculosis (MTB) from five
continents that were deposited at National Center for Biotechnology Information (NCBI).
Despite great diversity with respect to geographical point of isolation, genetic background and
drug resistance, the patterns of emergence of drug resistance were conserved globally. A
comprehensive bioinformatics pipeline was developed and systematic review from previous
studies were conducted to identify and quantify the frequency of the mutations associated with
resistance of isoniazid (INZ), rifampicin (RIF), ethambutol (ETB), pyrazinamide (PZA),
fluoroquinolone (FLQ), and streptomycin (ST). We have identified harbinger mutations that
often precede MDR and XDR (extensive drug resistance) in katG, inhA, rpoB, embB, pncA,
gyrA, gyrB and rpsL genes. It was observed that no mutations were found in 40% of the strains
studied and only 5% were MDR and 27% were pre-XDR and XDR. The harbinger mutations
identified may serve as an early warning signal that MDR may soon develop. Previous studies
showed that an overwhelming majority of MDR and XDR-TB had become cases of isoniazid
resistance prior to rifampicin resistance. Furthermore, targeting earliest-occurring mutations
could improve diagnosis of the patients and help implementation of precision medicine.
iPROMISE International Seminar 2017
28-29th November 2017, UiTM Puncak Alam
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Postgraduate Student’s Abstract
29thNOV
1015
Significant Modulation of Immune Response and Oxidative Stress during
Infection of Klebsiella Pneumoniae Profiled Using Metabolomics Analysis
Mohd Izwan Mohamad Yusof a, Mohd Salleh Rofiee a, Teh Lay Kek a,b, Mohd Zaki Salleha,b.
a Integrative Pharmacogenomics Institute, Level 3, FF3, Universiti Teknologi MARA (UiTM),
Puncak Alam Campus, Bandar Puncak Alam, 42300, Selangor, Malaysia. b Department of Pharmaceutical Life Sciences, Faculty of Pharmacy, Universiti Teknologi
MARA (UiTM), Puncak Alam Campus, Bandar Puncak Alam, 42300, Selangor, Malaysia
To better understand metabolic response in the host towards pathogenic infection, a Klebsiella
pneumoniae infected rat model was developed and investigated using metabolomics
approaches. K. pneumoniae was injected (i.v) into rats and the serum samples were collected at
three different time points (0 hours (pre-infection), 2 hours after infection (early infection) and
192 hours after infection (post-infection)). Thirteen (13) metabolites were characterized as
potential biomarkers related to K. pneumoniae infection. The potential biomarkers were derived
from nine (9) pathways which were found significantly perturbed in the host during K.
pneumoniae infection. According to the metabolic pathway analysis (MetPA), Tryptophan
metabolism was the most prominently influenced in K. pneumoniae-induced bacteremia
suggesting that significant modulation of the immune system activity had occurred during early
infection of K. pneumoniae. In addition, several metabolites indicate that the hosts were in
oxidative stress, inflammation and high energy demand state during early infection of K.
pneumoniae. Our findings provide perspective on the metabolites signatures together with
perturbated pathways related to the pathogenesis of bacteremia.
iPROMISE International Seminar 2017
28-29th November 2017, UiTM Puncak Alam
28
Acknowledgement
The organizing committee would like to express our gratitude towards the support,
commitment and cooperation rendered to ensure the success of iPROMISE International
Seminar (iPROMISe) 2017 for various individuals and entities within Universiti Teknologi
MARA as well as industrial partners as following:
Keynote Speaker & invited speakers
iPROMISE Research Fellows
iPROMISe 2017 organizing committee members