isolation and enrichment of soil bacteria
DESCRIPTION
Isolation and Enrichment of soil bacteria. Regina Gohl, K. Noreen Boyle 02/11/2011. University of Oldenburg - ICBM - Practical course Physiology of bacteria (Group B1) – final presentation. Regina Gohl, K. Noreen Boyle ICBM Uni Oldenburg 2. Introduction. - PowerPoint PPT PresentationTRANSCRIPT
Isolation and Enrichment of soil bacteria
Regina Gohl, K. Noreen Boyle
02/11/2011
University of Oldenburg - ICBM - Practical course Physiology of bacteria (Group B1) – final presentation
Introduction
•1 soil probe 2 experiments
Regina Gohl, K. Noreen Boyle ICBM Uni Oldenburg 2
Introduction Aims & Expectations Course results Summary
Isolation
Enrichment
? Medium variation
Dilution series
37 °CPasteurisationNitrogenNitrogen + yeastSaltSalt+yeastAcidicAlkaline
Aims and expectations
•Cultivation of soil bacteria•Determination of abundance•Comparison: fast vs abundant•Influence of different media
Introduction Aims & Expectations Course results Summary
Expectations Direct isolation
Enrichment
Types of bacteria
several one - few
Colony size small large
Growth slow fast
Conclusion abundant fast growing
Regina Gohl, K. Noreen Boyle ICBM Uni Oldenburg 3
Direct Isolation
Introduction Aims & Expectations Course results Summary
Cells/g soil
3.1 x 10^6
5.4 x 10^6
3.3 x 10^6
1.9 x 10^6
4.7 x 10^6
1.9 x 10^6
1.0 x 10^6
0.8 x 10^6
2.8 x 10^6 cells/g soil
~1 % of bacteria can be cultured in the lab
~ 10^9 bacteria/g soil
Less bacteria than expected
Regina Gohl, K. Noreen Boyle ICBM Uni Oldenburg 4
Direct Isolation
Introduction Aims & Expectations Course results Summary
Regina Gohl, K. Noreen Boyle ICBM Uni Oldenburg 5
Enrichment
Introduction Aims & Expectations Course results Summary
Treatment Oxidase test
Gram test Catalase test
Increased temperature
+ - - - - -
Pasteurisation + - - - + +
- nitrogen source - + +
-nitrogen source + yeast extract
+ + +
+ salt + + +
+ salt + yeast extract
+ + +
acidic + + +
alkaline + + +http://www.laborwissen.de/wiki/tiki-index.php?page=Katalase-Test
Regina Gohl, K. Noreen Boyle ICBM Uni Oldenburg 6
Enrichment
Introduction Aims & Expectations Course results Summary
Treatment Motility Growth Size Shape
Increased temperature
mobile fast large round, beige
Pasteurisation immobile slow small round, beige
- nitrogen source
immobile, rods
slow small white, smooth
- nitrogen source + yeast extract
mobile fast large yellow, smooth
+ salt - slow small small, round, white
+ salt + yeast extract
- fast large large, round, white
acidic cocci, immobile
slow small beige, bright
alkaline cocci, immobile
slow small beige, bright
Regina Gohl, K. Noreen Boyle ICBM Uni Oldenburg 7
Summary
•Results as expected•Variety of bacteria in soil•Different treatment growth / no growth
Introduction Aims & Expectations Course results Summary
- nitrogen source + yeast extract
- nitrogen source
Regina Gohl, K. Noreen Boyle ICBM Uni Oldenburg 8
Thank you for your attention