ISTA – Variety Committee

Day, 19 June 2010 Agenda Point (16:00-17:00)

... the Technical Committee for Species andVariety Testing

Report of …

Activities

Working program

Special projects

Berta Killermann

Committee members

Chair 1 Berta Killermann DE-Germany

Vice-chair 2 Ana Laura Vicario AR-Argentina

Members 3 Emanuela Casarini IT-Italy

4 Rainer Knoblauch DE-Germany

5 Kae-Kang Hwu TW-Taiwan

6 Cheryl Dollard CA-Canada

7 David Zhang FR-France

8 Chiara Delogu IT-Italy

9 Ulf Kjellström SE-Sweden

10 Eszter Timar HU-Hungary

11 Jerzy Drzewiecki PL-Poland

12 Hedwich Teunissen NL-Netherland

13 Savitha H. Reddy IN-India

14 Kalyn Brix-Davis US-United States

15 Wendy Carnegie CA-Canada

16 Michael Lovelace US-United States

Working Group – DNA-based methods

Leader 1 Emanuela Casarini IT-Italy Group leader

Co-workers 2 Ana Laura Vicario AR-Argentina soybean

3 Kae-Kang Hwu TW-Taiwan rice

4 Cheryl Dollard CA-Canada

5 Cesar Olsina AR-Argentina

6 Daniel Perry CA-Canada wheat

7 Marie-Jose Cote CA-Canada

8 Elisa Vieira BR-Brazil

9 Kalyn Brix-Davis US-United States

10 Kunusoth Keshavulu IN-India

11 Eun-Hee Soh KR-Korea

12 Kim Kenward CA-Canada

13 Benjamin Kaufman US-United States maize

14 Christelle Forzy FR-France

15 Chiara Delogu IT-Italy

16 Fengge Wang CN-China

17 Gaspar Malone AR-Argentina

Activities - WG DNA-based methods

2009

First ISTA Workshop on Molecular Markers for Variety Identity and Purity at the

University in Bologna, Italy (June 2009)

Comparative Tests (microsatellites - SSRs)

3rd CT for maize, wheat, soybean and rice were run

2010 and later

Comparative Tests (microsatellites , SSRs)

4th CT for soybean and wheat are running

First PT for DNA-based methods is planned for maize (under discussion)

handling of reference-material?

performance of the PT? (a classical PT or a performance based approach)

Development of an accreditation standard (including PTs)

a classical accreditation standard

performance based approach, like for GMO testing

Working Group – electrophoresis

Leader 1 Chiara Delogu IT-Italy

Co-workers 2 Rainer Knoblauch DE-Germany

3 Jerzy Drzewiecki PL-Poland

4 Savitha H. Reddy IN-India

5 Maria Alicia Loray AR-Argentina

6 Kalyn Brix-Davis US-United States

7 Kunusoth Keshavulu IN-India

8 Ravi Age IN-India

9 Yujia Wu US-United States

10 Berta Killermann DE-Germany

! We are looking for further co-workers !

Activities – WG electrophoresis

2009

Comparative Tests

Improvement of ISTA A-PAGE method for the verification of Triticum

Test leader Ana Laura Vicario (Argentina)

Design 3 wheat samples (ISTA method)

3 wheat samples (in-house method)

Participating labs Rainer Knoblauch - LTZ - Germany

M. Alicia Loray - INASE - Argentinia

Berta Killermann - LfL - Germany

Chiara Delogu - ENSE - Italy

Trevor Mazutinec - Bio Vision Seed Lab - Canada

Silvia Zanetti - ART - Switzerland

Jerzy Drzewiecki - IHAR - Poland

Status quo data analysis (STACOM)

forward a Rules proposal in 2010

Activities – WG electrophoresis

2010

Comparative Tests

SDS-PAGE for the discrimination of Triticum and xTriticosecale

Test leader Chiara Delogu (ENSE, Italy)

Participating labs we are looking for

Status quo test plan and the technical protocol will be presented

and discussed in the open VARCOM meeting

IEF for the discrimination of Triticum and xTriticosecale

Test leader not known at present

Status quo under discussion

SDS-PAGE, IEF for the discrimination of Festuca and Poa

Status quo under discussion

Activities – WG Electrophoresis

2010

ISTA Pre-Congress Workshop on Species and Variety Testing – electrophoresis

(Federal Plant Variety Office, Hanover, Lower Saxony, Germany)

Activities – WG Electrophoresis

Activities – WG Electrophoresis

Working Group – conventional methods

Leader 1 Michael Lovelace US-United States

Co-workers 1 Benno Voit DE-Germany

3 Wendy Carnegie CA-Canada

4 Berta Killermann DE-Germany

5 Gerry Hall UK-United Kingdom

6 ...

! We are looking for further co-workers !

Activities – WG conventional methods

2010 and later

Engagement in this field of work is of less interest

old fashioned methods but we urgently need this methods

Change in the leadership

where should we focus our activities in future ?

how can we handle this ?

how should we organize this working group ?

What can be done – how can we reach these aims?

development of a questionnaire concerning the need and development for

methods of variety control in laboratories and/or in field plots

catch new challenges (look for methods for testing herbicide tolerances on

seedlings by means of germination tests - greenhouse trials)

comparative tests

...

Projects for the future

Establishment of a database for an inventory of methods for species and variety

testing

electronical database (highly sophisticated)

working sheets (ring binder)

Revision of the existing four handbooks with the aim to have only one handbook

in loose-sheet format with 4 – 5 chapters and a harmonisation in the method

description

Electrophoresis Testing

Rapid Chemical Identification Techniques

Growth Chamber – Greenhouse Testing Procedures: Variety Identification

Laboratory Tests for Variety Determination with fungal Pathogens

DNA-based Methods (new capter?)

Organisation of further ISTA Workshops on Species and Variety Testing

list of possible local organizers (homepage)

list of lecturers (homepage)

safe time and money

Requests in 2009 / 2010

Can you give support (documents, statistics) for conducting an in-house

proficiency test round on hybrid purity testing (06/2009 India)

Assistance in testing maize seed intended for export, in the case when it is not

processed (07/2009 Serbia)

Assistance for bying an appropriate equipment for IEF (07/2009 Czech Republic)

Informations about the status quo and the future aims about the use of DNA

marker technology (SSR, etc.) for hybrid purity testing and why only IEF methods

are incorporated in the Rules – don`t you feel that with the recent advancements

and automation DNA technologies could be more rapid, reliable and economic?

(08/2009 India)

Assistance for bying the correct chemicals for A-PAGE (11/2009 Czech Republic)

How can we analyse the purity of rice varieties? (11/2009 Uganda)

Are methods existing to differentiate varieties of soybean seed lots related to

seedling or hypocotyl length? (12/2009 Argentina)

How can I test the measurement uncertainty in electrophoresis methods?

(02/2010 Serbia)

How can I become a member of the variety committee, especially in the working

group DNA-based methods?

Acknowledgement

Ana Laura VicarioKalyn Brix Davis

Chiara Delogu

Hedwich Teunissen

Cheryl Dollard

Kae-Kang Hwu

Emanuela Casarini

Eszter Timar

Rainer Knoblauch

Jerzy Drzewiecki

Savitha H. Reddy

Wendy Carnegie Ulf Kjellström

David Zhang

Berta Killermann

Michael Lovelace

Open Meeting of the Variety Committee

If you have any

questions

ideas

comments or …

please contact any of us or join the open meeting of the Variety Committee

Date: June 19th

Time: 19:00 – 20:30

Room: Meeting Room 2, 1st Floor

(Isabellensaal, rechts)

if you want to become a

member

co-worker of the Variety

Committee