joseph michael miano, ph.d. - university of...

Curriculum Vitae

September 13, 2013

Joseph Michael Miano, Ph.D.

I. Personal

Office Address Aab Cardiovascular Research Institute

University of Rochester School of Medicine & Dentistry

601 Elmwood Avenue, Box CVRI

Rochester, New York 14642

Mailing Address P.O. Box 76

Spencerport, NY 14559

Contact Numbers Office: (585)-276-9789

Fax: (585) 276-9830

E-mail: [email protected]

Citizenship: United States

Date of Birth June 30, 1962 in Syracuse, NY

Family Status Two children (Alexander, 19; Erika, 15)

II. Education/Training

Years

Degree Field of Study Institution Mentor(s)

1981-86 B.S. Biology/Physical

Education

State University of New

York at Cortland

David F. Berger, Ph.D.

Elliott B. Mason, Ph.D.

1986-88 M.S. Experimental

Pathology

New York Medical

College Alden V. Loud, Ph.D.

†

Piero Anversa, M.D.

1988-92 Ph.D. Experimental

Pathology

New York Medical

College

Michael B. Stemerman, M.D.

1992-95 Post-Doc Molecular/Dev

Biology

University of Texas M.D.

Anderson Cancer Center

Eric N. Olson, Ph.D.

III. Academic Appointments

2009-Present Tenured Associate Professor of Medicine and Associate Director of the

Cardiovascular Research Institute of the University of Rochester School of

Medicine and Dentistry, Rochester, New York

2009-Present Adjunct Professor of Xi’an Jiaotong University College of Medicine (China)

2007-2009 Tenured Associate Professor of Medicine and Interim Director of the

Cardiovascular Research Institute of the University of Rochester School of

Medicine and Dentistry, Rochester, New York

2003-2007 Associate Professor of Medicine in the Center for Cardiovascular Research of the

University of Rochester School of Medicine and Dentistry, Rochester, New York

1999-2003 Assistant Professor of Medicine and Pathology and Laboratory Medicine in the

Center for Cardiovascular Research, University of Rochester School of Medicine

and Dentistry, Rochester, New York

1995-1999 Assistant Professor in the Department of Physiology, Medical College of

Wisconsin, Milwaukee, Wisconsin

mailto:[email protected]

Curriculum Vitae Joseph M. Miano, Ph.D.

September 13, 2013

IV. Professional Consultantships

1998-2001 Introgen Therapeutics, Inc., Houston, Texas

2001-2012 Socratech Inc., Rochester, New York

V. Honors and Awards

1992 Martha Lucas Pate, PhD Memorial Award

1993 Post-Doctoral National Research Service Award, National Institutes of Health,

MD Anderson Cancer Center, Smooth Muscle Cell Differentiation

1997 Manitowoc Heart-A-Rama Research for Life Award-AHA, Wisconsin Affiliate

2001 Elected as Fellow of the American Heart Association

2003 Who’s Who in Medicine and Healthcare

2003-07 Established Investigator of the American Heart Association

2007 Inducted into the Gates-Chili High School Hall of Fame

2007 Tenured at the University of Rochester School of Medicine & Dentistry

2009- Adjunct Professorship, Xi’an University, China

VI. Professional Organizations

1988-present American Association for the Advancement of Science

1994-present North American Vascular Biology Organization

2000-2008 Society for Developmental Biology

2001-present Fellow of the American Heart Association

2004-2007

2009-2011

American Society for Biochemistry and Molecular Biology

International Mammalian Genome Society

VII. University Committee Assignments

1996-1999 Research Affairs Committee , Medical College of Wisconsin

1998-1999 Chair, Research Affairs Committee, Medical College of Wisconsin

1999-2003 Human Use of Radioisotopes Committee , University of Rochester

2000 Transgenic Facility Advisory Committee, University of Rochester

2000-2003 University Committee on Animal Resources, University of Rochester

2001-Present Reviewer, Strong Children’s Research Center Research Dev. Award

2001-2003 Member, Radiation Safety Advisory Board, University of Rochester

2001 Member, UCAR Forms Subcommittee

2001 Member, UCAR Breeding Subcommittee

2001 Member, Steering Committee for GEBS Cluster Realignment

2003-Present Member, Applications Committee for PHD GEBS Cluster

2004-Present Member, Steering Committee for Pathways of Human Disease Cluster

2004 Chair, CVRI Faculty Search Committee

2005 Member, Intellectual Property Advisory Committee

2005 Member, Vivarium Planning Committee for new CVRI

2006 Ad Hoc Committee Member for Promotions

2006 Member, Equipment Planning Committee for new CVRI

2007 Member, External Review of Center for Aging & Developmental Biology

2007-Present Functional Genomics Center, Advisory Group


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2007-Present

Member, Transgenic Mouse Core Advisory Committee

2007-2008 Member, Search Committee, Cardiology Chief

2008 Member, Search Committee, Senior Assoc Dean for Graduate Education

2008-Present Member, URMC Core Oversight Committee


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VIII. Local/National/International Assignments

1998-99 Study Section Member, American Heart Association, Affiliate, Great

America 2B

1999-2002 Study Section Member, American Heart Association, Affiliate, NE2

1999-2001 Study Section Member, American Heart Association, National, Vascular

Wall Biol I

1999 Co-Chair, Signaling and Vasc Biol, NAVBO, Washington, D.C.

2001-2004 Chair, Meritorious Awards Committee, NAVBO

2001-2004 Member, New York State AHA Affiliate Research Committee

2002-Present Reviewer, Science Foundation Ireland

2002-2007 Reviewer, Philip Morris External Research Program

2003-Present Reviewer, John Sealy Memorial Endowment Fund for Biomedical Res.

2004-2005 Member, Program Committee for 2005 NAVBO Meeting, Chicago, IL

2004 Chair, SMC Proliferation and Migration Session, AHA, New Orleans

2005 Chair, Vasculomics Session, NAVBO Annual Meeting, Chicago

2005 Ad hoc reviewer for NIH Special Emphasis Panel and R43 grants

2006-Present Member, External Advisory Committee for COBRE @ UN, Reno

2007 Co-Chair, NEA 5 (Gene Exp: Cardiovascular Development) AHA Study

Group

2008 Co-Chair, ATVB Plenary Session on “Stem Cells”, Atlanta, GE

2008 Member, NIH SEP (ZRG1 CVS-E) CV Dev and Stem Cells

2007-2009 Member, ATVB Program Committee

2009 Co-Chair, ATVB Session on Vascular Cell Dev and Differentiation

2009 AHA Science Classification Task Force

2009

2009-Present

Chair, Session on SMC-Specific Knockouts, FASEB Summer Research

Conference on SMC, Lucca, Italy (August 2-7)

Abstract Grader, American Heart Association

2012-Present Member, External Scientific Advisory Board, University of Virginia

Robert M. Berne Cardiovascular Research Center

2013- Ad Hoc Member, VCMB Study Section, National Institutes of Health

2012-2013 Conceived and Co-Organized 1st International MADS Box Conference held in

Canandaigua, NY, July 10-13. Raised more than $30,000.

IX. Community Service

1996-1998 Center for Science Excellence “Mini Medical School” Med College Wisconsin

1999-2001 Workshop Mentor for Victor Central School District

2000-Present Visiting Science Teacher, Our Mother of Sorrows Catholic School

2008-Present Community Outreach for the Aab CVRI


September 13, 2013

X. Ad Hoc Reviewer (past and present)

American Journal of Pathology Journal of Cellular Physiology

American Journal of Physiology, Cell Physiology Journal of Clinical Investigation

Anatomical Record Journal of Experimental Medicine

BMC Genomics

Cellular & Molecular Life Sciences

Cell Biochemistry & Biophysics

Journal of Molecular Biology

Journal of Vascular Research

Molecular Cellular Biology

Circulation

Developmental Biology

Developmental Cell

Disease Models & Mechanisms

Gastroenterology

Molecular Genetics & Genomics

Nature Methods

Physiological Reviews

PLoS One

PNAS

Human Molecular Genetics

Journal of Cell Biology

Science Signaling

Stem Cells

XI. Editorial Boards (past and present)

1998-2001 Journal of Vascular Research

2000-2007 American Journal of Physiology, Heart & Circulatory Physiology

2003-2007 Current Drug Targets - Cardiovascular and Haematological Disorders

2000-2007 Journal of Molecular & Cellular Cardiology

2004-2009

2000-2012

2007-2012

2010-2013

Journal of Biological Chemistry

Physiological Genomics

Associate Editor, Arteriosclerosis, Thrombosis & Vascular Biology

Consulting Editor, Circulation Research

1997-2013 Circulation Research

2012-present

2012-present

2014-present

Journal of Biomedical Research

Arteriosclerosis, Thrombosis & Vascular Biology

Cardiovascular Research

XII. Teaching Experience

1997-1999 Course Director, Problems in Molecular Physiology, Medical College of

Wisconsin

1998 Pathology for Basic Scientists , one lecture on Vascular Pathobiology,

Medical College of Wisconsin

1998 M4-Elective, Medical Problem Solving: Understanding Disease

Cardiovascular Basic Science Discussant, Medical College of Wisconsin

2000-2006 Coordinator, Signaling And Gene Expression (SAGE) Seminars

2001-2003 PHP440/BME 484 Topics in Vascular Biology, Univ. Rochester Med Ctr

2001-2003 Adult Inpatient Clerkship—Bed and Bench, Univ. of Rochester Med Ctr

2005 MD/PhD Program Scientific Reasoning in Medicine

2000-Present PTH593 Smooth Muscle Pathobiology, University of Rochester Medical

Center (3 hrs)

2002-Present PTH509/510 Pathways of Human Disease, Univ. of Rochester Medical

Center (3 hrs lecture, 1.5 hr paper discussion)

2007-Present Course Co-Director (2007 only) and lecturer (4 hrs), CVS-401,

Cardiovascular Biology and Disease

2008-Present GEN508 Genomics and Systems Biology (2.5 hrs)


September 13, 2013

XIII. Graduate Students (students obtaining PhD in Miano lab are in red)

Tammy Nuckolls Medical College of Wisconsin, SPUR, 1997

Heather Stefaniak Medical College of Wisconsin, SPUR, 1998

Shawn Jobe Medical College of Wisconsin, Dissertation Committee, 1995-97

James Bruce Medical College of Wisconsin, Dissertation Committee, 1998-1999

Christina Stafford Medical College of Wisconsin, Dissertation Committee, 1998-1999

Marcelo Nobrega Medical College of Wisconsin, Dissertation Committee, 1999-2000

Jeffrey Streb URMC, Dissertation Thesis Advisor, 2000-2004, Med School , SUNY

Stony Brook

Xinchun Pi URMC, Dissertation Committee, 2000-2004

Owen Felder URMC, Rotation Student, 2000

Owen Altland URMC, Rotation Student, 2000

Ruolan Han URMC, Rotation Student, 2001

Lan Tang (Linda) URMC, Rotation Student, 2002

Matthew Grunert URMC, Independent Research on Zebrafish Calponins, 2002

Hai-Ou Duan URMC, Dissertation Committee, 2002-Present

Ann Rossi URMC, Rotation Student 2002

Joseph Senn URMC, Dissertation Committee, 2002-2003

Tiefei Dong URMC, Dissertation Committee, 2003-2006

Lungkuo Tai URMC, Dissertation Committee, 2003-2006

Fred Strathmann URMC, Dissertation Committee, 2003-2008

David Lomb URMC, Dissertation Committee, 2003-2008

Ying Yang URMC, Dissertation Committee, 2003-2009

Lea Gunnell URMC, Dissertation Committee, 2004-Present

Ting-Hein Lee URMC, Dissertation Thesis Advisor, 2005-2008; Postdoc, UCSF

Ryan Hoefer URMC, Chair Dissertation Defense Committee, 2005

David Nagel URMC, Dissertation Committee, 2006-2008

Alayna Loiselle URMC, Dissertation Committee, 2006-2009

Vincent Fong URMC, Rotation Student 2006

Jody Davis URMC, Summer Student 2006

Jing Ni

Matthew Schoell

Kai Sun

Vincent Fong

Pauline Ocaya

Julie Smith

Robert Bell

Vivek Nanda

Erik Larsson

Xun Tang

Angela Aggrey

Zachary Brown

Dylan Pinto

Lesley Chapman

Lee Gerwitz

URMC, Chair Dissertation Defense Committee, 2006

URMC, Chair Dissertation Defense Committee, 2007

Medical College of Wisconsin, External Committee Member, 2007

URMC, Dissertation Committee, 2007-

Opponent, University of Orebro, Sweden 2006

URMC, Rotation Student 2008

URMC, Dissertation Committee, 2009-2011

URMC, Dissertation Thesis Advisor, 2009-2013; Postdoc, Stanford U

Opponent, University of Gothenburg, SWEDEN, on April 17, 2009

URMC, Summer Student, 2009

URMC, Dissertation Committee, 2010-Present

URMC, Undergraduate Student 2010-Present

URMC, Undergraduate Summer Scholar, 2010

URMC, Rotation Student, 2011

URMC, Summer Medical Student, 2011


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V. Post-doctoral Fellows

Jiyuan Chen, MD, PhD 10/16/99 – 3/15/03 Associate Professor, Division of

Cardiology, Zhongda Hospital, China

Hesheng Ou, MD, PhD 11/1/99 – 6/15/01 Professor In China

Vineet Dua, MD Sable Awardee, 2000-2001 Cardiologist, Strong Memorial Hospital

Qiang Sun, PhD 11/1/03-10/16/06 Post-Doc in Frankfurt, Germany

Yumei Yang, PhD 4/12/03-11/15/2004 Post-Doc at Emory University

Xiaochun Long, PhD 1/17/05-8/31/13 Ass’t Professor, Albany Medical College

Masaaki Imamura 4/1/08 – 5/14/10 Japanese Red Cross Otsu Hospital

Craig Benson, MD 3/30/09 – 6/30/12 Fellow, Beth Israel Deaconess Med Ctr

Qian Zhou, MD

Robert D Bell, PhD

Carolina Leimgruber

Pengtao Jiang

Yu Han

4/1/10 – 6/30/12

1/1/12 – 12/31/12

4/29/13 – 6/17/13

9/5/13 –

10/1/13 –

Post-Doc in University of Rochester

Senior Scientist, Pfizer

Post-Doc, Argentina

Post-Doc

Senior Research Associate

XV. Major Research Interests

1. Transcriptional control of smooth muscle cell differentiation

2. Human genome mining for CArG box regulatory elements and functional CArG-SNPs

3. Discovery and function of long noncoding RNAs

4. Development of genetic tools and models in mice

XVI. Invited Seminars (Local, National, and International)

1990-1996

1. “Early Induction of c-myc Following Balloon Injury in the Rat Aorta.” Federation Of

American Societies Of Experimental Biology (FASEB), Washington, D.C. April 20, 1990.

2. “Developmental Expression and Promoter Analysis of a SMC-Restricted Gene.” American

Heart Association Annual Meeting, Dallas, TX, November 12, 1994.

3. “Smooth Muscle Cell Marker Expression During Development” Federation Of American

Societies Of Experimental Biology (FASEB), Atlanta, GA. April 12, 1995.

4. “Smooth Muscle Cell Growth & Differentiation." Sponsored by Parke-Davis, Bruce

Markham, Ph.D, (Host). September 18, 1995.

5. "Molecular Control of Smooth Muscle Differentiation." Gordon Research Conference,

February 20, 1996.

1997

1. “Hierarchical Specificity of Smooth Muscle Marker Expression.” University of Wisconsin,

Gary Lyons (Host). May 13, 1997.

2. “Retinoids and Vascular Smooth Muscle Cell Biology.” Fourth Franz-Volhard Symposium,

Berlin, Germany, June 27, 1997

3. “Strategies for Studying Smooth Muscle Cell-Specific Gene Expression.” Medical College of Wisconsin,

Department of Anatomy and Cell Biology. Maya Sieber-Blum, Ph.D. ( Host). December 17, 1997.

4. “Retinoid Therapy: A New Pharmacological Approach to Limit Restenosis Following Angioplasty?”

University of Texas, M.D. Anderson Cancer Center, Houston, Texas. Scott Lippmann (Host). December 30,

1997.

1998

1. “Molecular Control of Smooth Muscle Calponin Expression.” International Workshop on

Airway and Vascular Smooth Muscle Function in Health and Disease. Sundance, Utah,October 30, 1998.


September 13, 2013

XVI. Invited Seminars (continued)

2. “Coronary Restenosis: A Translational Approach.” Cardiology Grand Rounds, Medical

College of Wisconsin. David Gutterman, M.D. (Host). November 19, 1998.

3. “Novel Approaches for the Treatment of Vascular Occlusive Diseases.” Introgen

Therapeutics, Inc., Houston, TX . Sunil Chada, PhD, (Host). December 10, 1998.

1999

1. “Novel Strategies for Adenoviral-Mediated Gene Transfer to Vascular Smooth Muscle

Cells.” Dysphagia Research Group, Medical College Of Wisconsin. Reza Shaker,

M.D.,(Host). January 5, 1999.

2. “New Approaches to Studying Vascular Smooth Muscle Cell Growth and Differentiation Control.” Center for

Cardiovascular Research, University of Rochester, Rochester, NY.

Bradford Berk, MD, Ph.D., (Host). February 12, 1999.

3. “Transcriptional Control of Smooth Muscle Calponin.” University of Calgary, Calgary,

Canada. Michael P. Walsh, Ph.D., (Host). March 5, 1999.

4. “New Approaches for the Prevention of Vascular Restenosis: Retinoids and SMC-Specific

Gene Targeting.” The New York Hospital Cornell Medical Center. Krishna Kandarpa

M.D. Ph.D., (Host). May 12, 1999.

5. “Retinoic Acid: A New Pharmacological Intervention for Vascular Occlusive Diseases?”

University of Texas Health Science Center at San Antonio. Tony Firulli, Ph.D., (Host).

May17, 1999.

6. “BAC on Track with the Transcriptional Regulation of Smooth Muscle Calponin.” Medical

College of Wisconsin. William Chilian, PhD, (Host). June 24, 1999.

7. “Experimental Therapies for Treating Cardiovascular Diseases - Contemporary Issues”

University of Rochester Medical Center, Jay H. Stein, M.D., moderator. December 17,

1999.

2000

1. “Transcriptional Regulation of Smooth Muscle Calponin.” FASEB Summer Research

Conferences: Smooth Muscle. Snowmass, Colorado, July 22, 2000.

2. “Using BACs to Delineate Transcriptional Regulatory Elements of the Smooth Muscle

Calponin Gene.” Columbia University. Peter Cserjesi, Ph.D., (Host). October 13, 2000.

3. “Models for Studying the Transcriptional Control of Smooth Muscle Calponin” Boston

Biomedical Research Institute. Albert Wang, PhD, (Host). November 1, 2000.

2001

1. “Retinoid-Mediated Effects on Smooth Muscle Cell Growth and the Vessel Wall’s Response

to Injury” University of Rochester Medical Center, Dept. Pathology, Oncology, and

Molecular Medicine. Steve Spitalnik (Host). February 5, 2001.

2. “BACing for Regulatory Elements Controlling Smooth Muscle Cell Differentiation”

University of Rochester Medical Center, Center for Aging and Developmental Biology.

David Pierce (Host). September 18, 2001.

3. “Retinoids and Vascular Smooth Muscle Cell Biology” New York Medical College, Dept.

Pathology. Caroline Smith (Host). October 3, 2001.

2002

1. “Retinoid Response Genes in Vascular Smooth Muscle Cells” Heidelberg – Berlin First Symposium on

Retinoids in Vascular and Renal Disease – new therapeutic indications? German Cancer Research Center.

Heidelberg, Germany. Jeurgen Wagner and Christos Zouboulis (Hosts). March 16, 2002.

2. “Retinoid Response Genes in Vascular Smooth Muscle” NAVBO Workshop on Nuclear Receptors, Salt Lake

City, UT, April 5, 2002.


September 13, 2013


3. “Vascular Smooth Muscle Cell Differentiation and Growth Control: Use of Genomics and Pharmaceutics”

University of Rochester Medical Center, Division of Neonatology, Michael O’Reilly (Host). June 11, 2002.

4. “Sifting a BAC for Regulatory Elements Controlling Smooth Muscle Calponin Expression” New York

Medical College, Dept. Pharmacology. Nader Abraham (Host). August 9, 2002.

5. “Genomico-Bioinformatic Strategies for Studying Vascular Smooth Muscle Cell Differentiation” University

of Rochester Medical Center. Socratech symposium on SMC. Berislav Zlokovic (Host). October 1, 2002.

6. “Characterization of a Unique Pulmonary Artery SMC Line” University of Rochester Medical Center.

Pulmonary Division Seminar. Patricia Sime (Host). October 8, 2002.

7. “BACing for Regulatory Elements Controlling Smooth Muscle Cell Differentiation” Morehouse School of

Medicine, Cardiovascular Research Institute. Gary Gibbons (Host). October 15, 2002.

8. “Mining a BAC for Regulatory Elements Controlling Smooth Muscle Calponin Expression” Maine Medial

Center, Center for Molecular Medicine. Tom Maciag (Host). October 24, 2002.

9. “Retinoids” American Heart Association, November 17, 2002

10. “Genomico-Bioinformatic Approach to Studying Smooth Muscle Cell Differentiation” University of Chicago,

Department of Medicine. Julian Solway (Host). November 18, 2002.

2003

1. “Retinoid Biology in Smooth Muscle Cells” University of Indiana, Center for

Cardiovascular Research. Keith March (Host). February 4, 2003.

2. “Genomico-Bioinformatic Approach to Studying Smooth Muscle Cell Differentiation”

University of Indiana, Department of Physiology. Paul Herring (Host). February 5, 2003.

3. “Genomico-Bioinformatic Approach to Studying Smooth Muscle Cell Differentiation”

University of North Carolina, Chapel Hill, Carolina Cardiovascular Biology Center. Cam Patterson (Host).

April 10, 2003.

4. “Genomico- Bioinformatic Approach to Studying Smooth Muscle Cell Differentiation”

4th

Annual ATVB Meeting, Washington, DC. May 9, 2003.

5. “Genomico-Bioinformatics and SMC Differentiation” FASEB Summer Conference on

Smooth Muscle Cells. Snowmass, CO. July 6, 2003.

6. “Genomico-Bioinformatics and SMC Differentiation” XIIIth International Symposium on Atherosclerosis.

Kyoto, Japan. October 1, 2003.

7. “Retinoid Effects in the Vessel Wall and the Smooth Muscle Cell Transcriptome” Role of Nuclear Receptors

in Cardiovascular Disease (Hot Topics in Endocrinology). Coronado, CA. October 10, 2003.

8. “Molecular Control of Smooth Muscle Differentiation” Vascular Biology Center in the Medical College of

Georgia. David Stepp/Brenda Lilly (Hosts). November 4, 2003.

9. “Transcriptional Regulation of Smooth Muscle Cell Differentiation” Department of Biology, University of

Rochester. Cheeptip Benyajati (Host). November 10, 2003.

10. “Genomico-Bioinformatics and SMC Differentiation” Department of Physiology, University of Tennessee.

Gadiparthi Rao (Host). November 20, 2003.

2004

1. “What’s New with the Control of Smooth Muscle Cell Differentiation?” Center for Cardiovascular

Sciences, Albany Medical College. John Schwarz, (Host). May 5, 2004.

2. “Genomics of Smooth Muscle Calponin Expression” Department of Pharmacology & Physiology, Drexel

University. Robert Moreland (Host). May 24, 2004.

3. “Transcriptional control of vascular smooth muscle differentiation” Department of Pathology, University

of Rochester School of Medicine. Robert Mooney (Host). June 14, 2004.

4. “Molecular Control of Smooth Muscle Cell Differentiation” Distinguished Scientist Seminar Series,

University of South Alabama College of Medicine. Thomas Lincoln (Host). October 21, 2004.


September 13, 2013


5. “Role of SRF-Myocardin in Muscle Cell Differentiation” Department of Biochemistry, Medical College of

Wisconsin. Ravi Misra (Host). October 27, 2004.

2005

1. “Investigating Vascular Smooth Muscle Cell Pathobiology in the 21st Century” Biology Department,

SUNY Cortland. Larry Klotz, (Host). April 6, 2005.

2. “Serum Response Factor and the Mammalian CArGome” Department of Pathology, Yale University, Wang

Min (Host). June 7, 2005.

3. “Serum Response Factor and the Mammalian CArGome” Department of Genetics, University of

Pennsylvania, Christian Stoeckert (Host). June 20, 2005.

4. “SRF and Mammalian CArGome” Department of Physiology, Texas A&M University, Emily Wilson

(Host). September 8, 2005.

5. “SRF and the CArGome” Muscling Through the Ages Symposium at University of Texas Southwestern

Medical School, Eric Olson (Host). September 24, 2005.

2006

1. “SRF and the CArGome” Department of Pharmacology and Physiology, University of Nevada, Reno, Dean

Burkin, PhD (Host). March 9, 2006.

2. “SRF and the CArGome” Department of Molecular and Cellular Biology, Roswell Park Cancer Institute,

Kenneth Gross, PhD (Host). April 20, 2006.

3. “SRF and the CArGome” University of Virginia, Charlottesville, Gary Owens, PhD (Host). May 18,

2006.

4. “Role of Serum Response Factor and the CArGome in Smooth Muscle Cell Differentiation” FASEB

Summer Conference on Smooth Muscle Cells. Snowmass, CO. July 30, 2006.

5. “Biology of a Retinoid-Induced A-Kinase Anchoring Protein” University of South Alabama College of

Medicine. Thomas Lincoln (Host). November 15, 2006.

6. “Vascular Smooth Muscle Cell Differentiation in Danio rerio” University of Örebro (Sweden). Leif

Eriksson (Host). December 7, 2006.

7. “Retinoid Biology in Smooth Muscle Cells and the Vessel Wall” University of Örebro (Sweden). Allan

Sirsjö (Host). December 8, 2006.

2007

1. “SRF and Myocardin in Smooth Muscle Differentiation and Alzheimer’s Angiopathy” Wayne State

University. Li Li (Host). May 25, 2007.

2. “Bridging Bioinformatics with Experimental Biology” URMC, Dept Biostatistics and Computational

Biology. Jim Cavenaugh (Host). September 21, 2007.

3. “Diverse Roles for SRF-Myocardin in Muscle Biology” Dartmouth Medical School. Kathy Martin (Host).

October 3, 2007.

4. “SRF-Myocardin in Development and Disease” Dept. Pathology, University of Rochester School of

Medicine and Dentistry. Dan Ryan (Host). November 12, 2007.

2008

1. “Diverse Roles for SRF-Myocardin in Muscle Biology” Department of Biochemistry, Vermont University

Medical School. Robert Kelm (Host). March 28, 2008.

2. “Diverse Roles for SRF-Myocardin in Muscle Biology” Committee on Cell Physiology, University of

Chicago. Nickolai Dulin (Host). May 2, 2008.

3. “Diverse Roles for SRF-Myocardin in Muscle Biology” Division of Cardiology, Emory University School

of Medicine. Kathy Griendling (Host). September 8, 2008.

4. “Diverse Roles for SRF-Myocardin in Muscle Biology” Herman B. Wells Center for Pediatric Research,

Indian University. Anthony Firulli (Host). October 9, 2008.


September 13, 2013


5. “Diverse Roles for SRF-Myocardin in Muscle Biology” Institut für umweltmedizinische Forschung of

the University of Düsseldorf, Germany. Judith Haendeler (Host). November 19, 2008.

2009

1. “Diverse Roles for SRF-Myocardin in Muscle Biology” Department of Pathology, University of Alabama

at Birmingham. Yabing Chen (Host). January 15, 2009.

2. “Diverse Roles for SRF-Myocardin in Muscle Biology” Department of Physiology & Pharmacology,

University of Georgia, Athens. Shiyou Chen (Host). March 30, 2009.

3. “SRF-Myocardin: From Genome to Disease” Department of Medicine, Research in Medicine Seminar.

University of Rochester School of Medicine & Dentistry. David Bushinsky (Host). April 8, 2009.

4. “Diverse Roles for SRF-Myocardin in Muscle Biology” The Sahlgrenska Academy of the University of

Gothenburg, Sweden. Per Lindahl (Host). April 16, 2009.

5. “Retinoid Biology in Vascular Smooth Muscle Cells” Department of Medicine, Columbia University.

William Blaner (Host). June 3, 2009.

6. “Role of SRF and Myocardin in Muscle Biology” Department of Medicine, New York University.

Edward Fisher (Host). October 2, 2009.

7. “Retinoid Biology in Vascular Smooth Muscle Cells” Institute of Cardiovascular Science, Peking

University Health Science Center. Nanping Wang (Host). October 8, 2009.

8. “Role of SRF and Myocardin in Muscle Biology” Xi'an Jiaotong University College of Medicine (China).

Dr. Hong Yan (Dean of Medical School, Host). October 9, 2009.

9. “Role of SRF and Myocardin in Muscle Biology” Department of Medicine, Cardiovascular Research

Center, Albert Einstein College of Medicine. Nicholas Sibinga (Host). October 20, 2009.

10. “Mechanisms of Smooth Muscle Cell Differentiation” AHA Sessions. November 17, 2009.

11. “Diverse Roles for SRF-Myocardin in Muscle Biology” Cardiovascular Research Center, University of

Kentucky. Dennis Bruemmer (Host). December 4, 2009.

2010

1. “Transcriptional Control of Vascular Smooth Muscle Cell Phenotypes” Department of Biomedical

Sciences, Cornell University. Michael Kotlikoff (Host). February 23, 2010.

2. “Micro RNAs and SMC Phenotype” Keynote Lecture at European Young Physiological Society,

Copenhagen University. Maria Gomez (Host). March 27, 2010.

3. “Cardiovascular Gene Expression Control Through SRF-Myocardin” Cardiology Interest Group, Cornell

University. Joshua Novy (Host). April 29, 2010.

4. “Transcriptional and Post-Transcriptional Control of Smooth Muscle Cell Differentiation” Department of

Pharmacology, University of Vermont. Karen Lounsbury (Host). May 13, 2010.

5. “Diverse Roles for SRF and Myocardin in Muscle Biology” Department of Cell Biology, SUNY Downstate

Medical Center. Brahim Chaqour (Host). May 26, 2010.


Pathology, University of Chicago. Lucia Shuger (Host). October 26, 2010.

2011

1. “Defining the Human CArGome” University of Rochester Medical Center, TIGR. Tom Mariani (Host).

March 13, 2011.

2. “SRF-Myocardin in Biology and Disease” Department of Biomedical Sciences, Dalton Cardiovascular

Research Center, University of Missouri-Columbia. Doug Bowles (Host). May 12, 2011.


Pharmacology and Toxicology, Virginia Commonwealth University. Ningjun Li (Host). May 17, 2011.

4. “SRF-Myocardin and the CArGome” Department of Molecular & Human Genetics, Baylor College of

Medicine. Antony Rodriguez (Host). October 4, 2011.


September 13, 2013

XVI. Invited Seminars (continued) 2012

1. “Genomic Regulation of Vascular Smooth Muscle Cell Phenotype” Keystone Symposia in Big Sky,

Montana: Molecular Basis of Vascular Inflammation and Atherosclerosis. Edward Fisher (Host). March

28, 2012.

2. “SRF and Myocardin in the Regulation of Vascular Smooth Muscle Cell Phenotype” Nanjing Medical

University, China. Guoyong Yin (Host). May 23, 2012.

3. “Regulatory Control of Vascular Smooth Muscle Cell Phenotype” The 6th

Oriental Congress of

Cardiology, Shangahi, China. Pinglin Gao (Host). May 25, 2012.

4. “Scientific Publishing and Integrity: Perspectives from a Journal Editor” Nanjing Medical University,

Nanjing, China. Yong Xu (Host). November 8, 2012.

5. “Molecular Control of Vascular Smooth Muscle Cell Phenotypic Adaptation” Jinling International

Symposium on Cardiovascular Translational Medicine. Nanjing, China. Qi Chen (Host). November 9,

2012.

2013

1. “Mining the Genome for Snippets of Gold” Aab Cardiovascular Research Institute, University of

Rochester School of Medicine & Dentistry. Eric Small (Host). February 6, 2013.

2. “Long Non-Coding RNA-Mediated Control of Human Vascular Cell Phenotypes” Symposium on Vascular

Signaling Mechansims, University of Lund, Sweden. Per Hellstrand (Host). June 14, 2013.

3. “Mining the Human Genome for Nuggets of Gold” Transcriptional Interest Group & Research. University

of Rochester. Tom Mariani (Host). August 1, 2013.

4. “Mining the Human Genome for Nuggets of Gold” Department of Neurosurgery. University of Rochester.

Rashid Deane (Host). August 2, 2013.

5. “Mining the Human Genome for Nuggets of Gold” Cardiovascular Research Institute. Morehouse School

of Medicine. Minerva Garcia-Barrio (Host). September 12, 2013.

6. “Mining the Human Genome for Nuggets of Gold” Division of Cardiology, Emory School of Medicine.

Alejandra San Martin (Host). September 16, 2013.

7. “Role of Medial SMC in Response to Vascular Injury” American Heart Association. Dallas, TX. October

18, 2013.

2014

1.


September 13, 2013

XVII. Media Features

1. Milwaukee Journal Sentinel, “Wrinkle Cream May Treat Arteries.” February 7, 1996.

2. Rochester Democrat and Chronicle, “Teachers Learn How to Inspire Future Scientists.” June 1, 2000.

3. Rochester Democrat and Chronicle, “UR Researchers Cheer new Chromosome Map.” July 2, 2000.

4. WOKR, Channel 13 Health News, “Zebrafish Used to Study Cardiovascular Research.” February 19,

2004.

5. Rochester Democrat and Chronicle, “UR Sleuths on DNA Mission.” April 15, 2006.

6. Rochester Democrat and Chronicle, “UR Heart Researchers to Get Own Building.” August 22, 2007.

7. LaBarge Media, “DNA and the Origins of Life.” January 20, 2011.


September 13, 2013

XVIII. Grant Support

Previous Research Support

Principal Investigator, “Identification of Smooth Muscle Cell-Specific Transcription Factors.” Medical College of

Wisconsin New Faculty Grant. $7,500, 11/15/95-11/14/96.

Principal Investigator, :“Retinoid-Mediated Smooth Muscle Cell Growth Control. American Heart Association

Grant-In-Aid, Wisconsin Affiliate. Total Direct Costs, $60,000; 7/96-6/98.

Principal Investigator,“A Cell-Specific Promoter-Based Approach to Targeting Transgene Expression in Normal

and Diseased Blood Vessels.” The Charles Culpeper Foundation. Total Direct Costs, $22,438; 1/1/98-12/31/98.

Principal Investigator, (10% effort). “Retinoid-Mediated Smooth Muscle Cell Growth and Differentiation

Control.” AHA Scientist Development Grant. Total Direct Costs, $260,000; 1/1/98-12/31/01.

Principal Investigator, (10% effort). “Role of Tissue Transglutaminase in Retinoid-Mediated

Smooth Muscle Cell Responses.” AHA Grant-In-Aid (Affiliate). Total Direct Costs, $180,000; 7/1/01-12/31/02

(Grant relinquished 12/02 to accept funding for AHA Established Investigator Award on 1/1/03.)

Applicant Sponsor, (0% effort). “Local Delivery of Retinoid-Related Compounds to the Injured Vessel Wall.”

AHA Postdoctoral Fellowship for Jiyuan Chen. Total Direct Costs, $103,605; 7/1/00-6/30/03

Applicant Sponsor, (0% effort). “Transcriptional Regulation of Myocardin Gene Expression.” AHA Postdoctoral

Fellowship for Xiaochun Long. Total Direct Costs, $76,000; 7/1/06 – 06/30/08.

Principal Investigator, (10% effort). “Transcriptional and Functional Analysis of a Highly Restricted, CArG-

Independent Vascular Smooth Muscle Cell Gene.” AHA Established Investigator Award. Total Direct Costs,

$454,545; 01/01/03 – 12/31/07

Principal Investigator, (35% effort). “Analysis of a Retinoid-Induced Tumor Suppressor in

SMC.” NIH R01 HL70077. Total Direct Costs, $900,000; 07/01/03-06/30/07.

Core B Director, Histology, (10% effort). “Vascular Inflammation and Atherosclerosis.” NIH PO-1 HL01-

077789. Total Direct Costs in Year 1, $103,450; 8/1/05 – 07/31/10.

Principal Investigator, (15%). “SRF/MYOCD: New Targets in Alzheimer’s Neurovasculature.” Phase II STTR,

AG026950-02. Total Direct Costs, $172,664; 06/15/08 – 05/31/11.

Principal Investigator, (20% effort). “Molecular Control of Smooth Muscle Calponin

Expression.” NIH R01-HL62572. Total Direct Costs, $900,000; 04/01/99 – 06/30/12.

Principal Investigator, (25%). “Bifunctionality of Myocardin in Myogenesis.” NIH R01-HL091168.

Total Direct Costs, $1,000,000; 07/01/08 – 06/30/12.

Mentor of an AHA Postdoctoral grant to Dr. Robert D. Bell entitled, “Myocardin Regulation of Cerebral Blood

Flow.” Total Direct Costs, $80,000; 07/01/12 – 01/01/13.

Mentor of an AHA National SDG application to Dr. Xiaochun Long entitled, “Role of TGF microRNAs in

Smooth Muscle Cell Phenotype.” Total Direct Costs, $280,000; 07/01/10 – 06/30/14.


September 13, 2013

Active Research Support

Principal Investigator, (30% effort). “Regulation and function of AKAP12A in the vessel wall.” NIH R01-

HL112793-01. Total Direct Costs, $ 1,498,020 (sub-award to Ed Fisher, NYU); 08/01/13 – 03/31/17.

Principal Investigator, (25% effort). “Regulation and function of myocardin in vascular pathobiology.” NIH R01-

HL117907-01. Total Direct Costs, $1,250,000; 08/01/13 – 03/31/17.

Principal Investigator, (5% effort). “Cardiovascular CArGome function and variation.” AHA Founders Affiliate

Grant-in-Aid 13GRNT14260004 . Total Direct Costs, $180,000; 01/01/13 – 12/31/15.

Co-Investigator with David Dean (PI), (15% effort). “DNA nuclear import sequences for cell-specific gene and

drug delivery.” NIH R01-GM094228. Total Direct Costs to Miano, $173,605; 07/01/10 – 06/30/15.

Co-Investigator with Seungil Ro (PI), (10% effort). “Roles of DNA methylation in gastrointestinal smooth muscle

cells.” NIH R01-DK094886. Total Direct Costs to Miano, $100,000; 04/01/12 – 03/31/17.

Co-Investigator with Gen Jin (PI), (10% effort). "Molecular regulation of angiogenesis." 1R01HL114570-01.

Total Direct Costs to Miano, $80,000; 07/01/12 – 06/30/17.

Co-Investigator with Eric Small (PI), (5% effort). “Regulation of epicardial cell differentiation during

development and disease.” NIH 1R01HL120919-01, $1,250,000; 09/01/13 – 08/31/18.

Pending Support


September 13, 2013

IX. Publications (* Corresponding Author)

1. Miano JM, Holland JA, Pritchard KA, Rogers NJ, Stemerman MB. Low density lipoprotein-mediated

endothelial cell perturbation: Effects on endothelial cell eicosanoid metabolism. In (Samuelsson,B. and P.K.Y.

Wong, Eds.) Advances in Prostaglandin, Thromboxane and Leukotriene Research. Raven Press, New York,

Vol. 19, pp. 248-254, 1989.

2. Miano JM*, Tota RR, Vlasic N, Danishefsky K, Stemerman MB. Early proto-oncogene expression in rat

aortic smooth muscle cells following endothelial removal. Am.J.Pathol. 137:761-765, 1990.

3. Miano JM. Smooth muscle cell immediate early gene expression following vascular injury: Relationship to

growth factor gene induction and smooth muscle cell growth. Dissertation. University Microfilms Inc., Ann

Arbor, MI 1992.

4. Miano JM, Vlasic N, Tota RR Stemerman MB. Smooth muscle cell immediate-early gene and growth factor

activation follows vascular injury: A putative in vivo mechanism for autocrine growth. Arterioscler.Thromb.

13:211-219, 1993.

5. Miano JM, Vlasic N, Tota RR, Stemerman MB. Localization of Fos and Jun proteins in rat aortic smooth

muscle cells after vascular injury. Am.J.Pathol. 142:715-724, 1993.

6. Pritchard KA, O'Banion MK, Miano JM, Vlasic N, Bhatia UG, Young DA, Stemerman MB. Acute

expression of a novel cyclooxygenase gene in rat vascular smooth muscle cells in vitro and in vivo.

J.Biol.Chem. 269:8504-8509, 1994.

7. Martin JF, Miano JM, Hustad CM, Copeland NG, Jenkins NA, Olson, EN. A Mef2 gene that generates a

muscle-specific isoform via alternative mRNA splicing. Mol. Cell.Biol. 14:1647-1656, 1994.

8. Miano JM, Cserjesi P, Ligon KL, Periasamy M, Olson EN. Smooth muscle myosin heavy chain marks

exclusively the smooth muscle lineage during mouse embryogenesis. Circ.Res. 75:803-812, 1994.

9. Miano JM, Firulli AB, Olson EN, Hara P, Giachelli CM Schwartz SM. Restricted expression of homeobox

genes distinguishes fetal from adult human smooth muscle cells. Proc.Natl.Acad.Sci., USA. 93:900-905, 1996.

10. Li L, Miano JM, Cserjesi P, Olson EN. SM22, a marker of adult smooth muscle, is expressed in multiple

myogenic lineages during embryogenesis. Circ.Res. 78:188-195, 1996.

11. Firulli AB, Miano JM, Bi W, Johnson D, Casscells W, Olson EN, Schwarz JJ. Myocyte enhancer binding

factor-2 expression and activity in vascular smooth muscle cells: association with the activated phenotype.

Circ.Res. 78:196-204, 1996.

12. Li L, Miano JM, Mercer B, Olson EN. Expression of the SM22 promoter in transgenic mice provides

evidence for distinct transcriptional regulatory programs in vascular and visceral smooth muscle cells. J. Cell

Biol. 132:849-859, 1996.

13. Miano JM,* and Olson EN. Expression of the smooth muscle cell calponin gene marks the early cardiac and

smooth muscle cell lineages during mouse embryogenesis. J.Biol.Chem. 271:7095-7103, 1996.


September 13, 2013

14. Miano JM,* Topouzis S, Majesky MW, Olson EN. Retinoid receptor expression and all-trans

retinoic acid-mediated growth inhibition in vascular smooth muscle cells. Circulation. 93:1886-1895, 1996.

15. Miano JM,* Krahe R, Garcia E, Elliott JM, Olson EN. Expression, genomic structure and high resolution

mapping to 19p13.2 of the human smooth muscle cell calponin gene. Gene. 197:215-224, 1997.

16. Rao RS, Miano JM, Olson EN, Seidel CL. The A10 cell line: a model for neonatal, neointimal, or

differentiated vascular smooth muscle cells? Cardiovasc.Res. 36:118-126, 1997.

17. Koike G, Miano JM, Vanvooren P, Shiozawa M, Szpirer C, Jacob HJ. Mapping of the rat SM22 gene to

chromosome 8q24. Mamm. Genome. 9:76-77, 1998.

18. Firulli AB, Han D, Kelly-Roloff L, Koteliansky VE, Schwartz SM, Olson EM, Miano JM.* A comparative

molecular analysis of four rat smooth muscle cell lines. In Vitro Cell.Dev.Biol.Anim. 34:217-226, 1998.

19. Zilverman A, Dave V, Miano JM, Olson EN, Periasamy M. An evolutionary conserved promoter region

containing CArG-like elements is crucial for smooth muscle myosin heavy chain gene expression. Circ.Res.

82:566-575, 1998.

20. Miano JM,* Kelly LA, Artacho CA, Nuckolls TA, Piantedosi R, Blaner WS. All-trans retinoic acid reduces

neointimal formation and promotes favorable geometric remodeling of the rat carotid artery following balloon

withdrawal injury. Circulation. 98:1219-1227, 1998.

21. Clifford JL, Miano JM, Lippman SM. Retinoids and interferons as antiangiogenic cancer drugs. In:

Antiangiogenic Agents in Cancer. Teicher, B.A., Ed., Humana Press, 1999. 355-370.

22. Miano JM,* Garcia E, Krahe R. Radiation hybrid (RH) mapping of human smooth muscle-restricted genes.

In: Molecular Biology of Vascular Disease. Baker, A.H., Ed., Humana Press, 1999. 25-35.

23. Kowal RC, Richardson JA, Miano JM, Olson EN. EVEC: A novel epidermal growth factor-like repeat-

containing protein expressed by embryonic and diseased adult vasculature. Circ.Res. 84:1166-1176, 1999.

24. Miano JM, and Berk BC. NAB2: A transcriptional brake for activated gene expression in the vessel wall?

Am.J.Pathol. 155:1009-1012, 1999.

25. Nobrega MA, Shiozawa M, Koike G, Jacob HJ, Miano JM.* Gene structure, promoter analysis, and

chromosomal mapping of the rat smooth muscle calponin gene. Mamm. Genome. 11:115-119, 2000.

26. Molkentin JD, Antos C, Mercer B, Taigen T, Miano JM, Olson EN. Direct activation of a GATA6 cardiac

enhancer by Nkx2.5: Evidence for a reinforcing regulatory network of Nkx2.5 and GATA transcription factors

in the developing heart. Dev.Biol. 217:301-309, 2000.

27. Miano JM, Carlson MA, Spencer JJ, Misra RP. Serum response factor-dependent regulation of the smooth

muscle calponin gene. J.Biol.Chem., 275:9814-9822, 2000.

28. Camoretti-Mercado B, Liu HW, Halayko AJ, Forsythe SM, Kyle JW, Li B, Fu Y, McConville J,

Kogut P, Vieira JE, Patel NM, Hershenson MB, Fuchs E, Sinha S, Miano JM, Parmacek MS,

Burkhardt JK, Solway J. Physiologic control of smooth muscle-specific gene expression through


September 13, 2013

regulated nuclear translocation of serum response factor. J.Biol.Chem., 275:30387-30393, 2000.

29. Miano JM,* and Berk BC. Retinoids: Versatile biological response modifiers of vascular smooth muscle

phenotype. Circ.Res., 87:355-362, 2000.

30. Ou H, Haendeler J, Aebly MR, Kelly LA, Cholewa BC, Koike G, Kwitek-Black A, Jacob HJ, Berk BC, Miano

JM.* Retinoic acid-induced tissue transglutaminase and apoptosis in vascular smooth muscle cells. Circ.Res.,

87:881-887, 2000.

31. Chen J, Maltby KM, Miano JM.* A novel retinoid-response gene set in vascular smooth muscle cells.

Biochem.Biophys.Res.Comm. 281:475-482, 2001.

32. Miano JM,* Thomas S, Disteche CM. Reversible expression and chromosomal mapping of the mouse smooth

muscle calponin gene. Mamm. Genome. 12:187-191, 2001.

33. Miano JM,*and Berk BC. Retinoids: New Insight into Smooth Muscle Cell Growth Inhibition.

Arterioscler.Thromb.Vasc.Biol., 21:724-726, 2001.

34. Dulin NO, Orlov SN, Kitchen CM, Voyno-Yasenetskaya TA, Miano JM.* G protein-coupled receptor-

activation of the smooth muscle calponin gene. Biochem.J., 357:587-592, 2001.

35. Chen J, Streb JW, Maltby KM, Kitchen CM, Miano JM.* Cloning of a novel retinoid-inducible serine

carboxypeptidase from vascular smooth muscle cells. J.Biol.Chem. 276:34175-34181, 2001.

36. Frame MD, Miano JM, Yang J, Rivers RJ. Localized adenovirus-mediated gene transfer into vascular smooth

muscle in the hamster cheek pouch. Microcirculation, 8:403-413, 2001.

37. Miano JM,* Kitchen CM, Chen J, Maltby KM, Kelly LA, Weiler H, Krahe R, Ashworth

LK, Garcia E.

Human smooth muscle calponin expression in transgenic mice revealed with a bacterial artificial chromosome.

Am.J.Physiol. Heart Circ.Physiol., 282:H1793-1803, 2002.

38. Miano JM. Mammalian smooth muscle differentiation: origins, markers and transcriptional control. Results

Probl. Cell Differ. 38:39-59, 2002.

39. Chen J, Kitchen CM, Streb JW, Miano JM.* Myocardin: a component of a molecular switch for smooth

muscle cell differentiation. J.Mol.Cell.Cardiol., 34:1345-1356, 2002.

Editorialized in AB Firulli “Another hat for myocardin.” J.Mol.Cell.Cardiol., 34:1293-1296, 2002.

40. Miano JM. Cardiac-specific gene expression: a HANDful of factors. J.Mol.Cell.Cardiol., 34:1287-1291,

2002.

41. Streb JW and Miano JM.*

Retinoids: pleiotropic agents of therapy for vascular diseases? Curr. Drug Targets

– Cardiovasc.Haematol.Disord., 3:31-57, 2003.

42. Davis A, Hogarth K, Fernandes D, Solway J, Niu J, Kolenko V, Luttrell L, Browning D, Miano

J.M, Orlov S, Dulin NO. Functional significance of protein kinase A activation by endothelin-1 and ATP:

negative regulation of SRF-dependent gene expression by PKA. Cell.Signal., 15:597-604, 2003.


September 13, 2013

43. Chen J, Chada S, Mhashilkar A,

Miano JM,

* Tumor Suppressor MDA-7/IL-24 Selectively Inhibits Vascular

Smooth Muscle Cell Growth and Migration. Molecular Therapy, 8:220-229, 2003.

44. Miano JM, Serum response factor: toggling between disparate programs of gene expression.

J.Mol.Cell.Cardiol., 35:577-593, 2003.

45. Aizawa T, Wei H, Miano JM, Abe J-i, Berk BC, Yan C*. Anti-inflammatory effects of NO/cGMP are

mediated by PDE3 Inhibition in vascular smooth muscle cell. Circ.Res., 93:406-413, 2003.

46. Jiang M, Mezentsev A, Kemp R, Byun K, Falck JR, Miano JM, Nasjletti A, Abraham NG, Laniado-

Schwartzman M.* Smooth Muscle-Specific Expression of CYP4A1 Induces Endothelial Sprouting in Renal

Arterial Microvessels. Circ.Res., 94:167-174, 2004.

47. Zhang R, Al-Lamki R, Bai L, Streb JW, Miano JM, Bradley J, Min W*. Thioredoxin-2 inhibits mitochondria-

located ASK1-mediated apoptosis in a JNK-independent manner. Circ.Res, 94:1483-1491, 2004.

48. Hogarth DK, Sandbo N, Taurin S, Kolenko V, Miano JM, Dulin NO*. Dual role of PKA in phenotypic

modulation of vascular smooth muscle cells by extracellular ATP. Am.J.Physiol. Cell Physiol. 287:C449-

C456, 2004.

49. Miano JM, Channeling to myocardin. Circ.Res. 95:340-342, 2004.

50. Miano JM,*, Ramanan N, Georger MA, de Mesy Bentley KL, Emerson RL, Balza RO, Jr,

Xiao Q, Weiler H, Ginty DD, Misra RP.* Restricted inactivation of serum response factor to the

cardiovascular system. Proc.Natl.Acad.Sci., USA., 101:17132-17137, 2004.

51. Streb JW, Kitchen CM, Gelman IH, Miano JM.* Multiple promoters direct expression of three AKAP12

isoforms with distinct tissue and subcellular distribution profiles. J.Biol.Chem., 279:56014-56023, 2004.

52. Streb JW and Miano JM.* AKAP12: An atypical serum response factor-dependent target gene.

J.Biol.Chem., 280:4125-4134, 2005.

53. Zlokovic BV*, Deane R, Sallstrom J, Chow N, Miano JM. Neurovascular pathways and Alzheimer amyloid

-peptide. Brain Pathol., 15:78-83, 2005.

54. Blaxall BC, Miano JM, Berk BC.* Angiotensin II: a devious activator of mineralocorticoid receptor-

dependent gene expression. Circ.Res., 96:610-611, 2005.

55. Streb JW and Miano JM.* Cross-species sequence analysis reveals multiple charged residue-rich domains that

regulate nuclear/cytoplasmic partitioning and membrane localization of A kinase anchoring protein 12

(SSeCKS/Gravin). J.Biol.Chem., 280:28007-28014, 2005.

56. Ping Q, Ritchie R, Fu Z, Cao D, Cumming J, Miano JM, Wang DZ, Li HJ, Li L.* Myocardin enhances

Smad3-mediated TGF-1 signaling in a CArG box-independent manner: Smad binding site (SBE)

is a critical cis-element for SM22 transcription in vivo. Circ.Res., 97:983-991, 2005.

57. Sun Q, Chen G, Streb JW, Long X, Yang Y, Stoeckert CJ, Jr, Miano JM.* Defining the

mammalian CArGome. Genome Res., 16:197-207, 2006.


September 13, 2013

58. Rensen SSM, Niessen PMG, Long X, Doevendans PA, Miano JM, van Eys GJJM. * Contribution of serum

response factor and myocardin to transcriptional regulation of smoothelins. Cardiovasc.Res., 70:136-145,

2006.

59. Lee THD, Streb JW, Georger MA, Miano JM.* Tissue expression of the novel serine carboxypeptidase

Scpep1. J.Histochem.Cytochem., 54:701-711, 2006.

60. Nagel DJ, Aizawa T, Jeon K-I, Liu W, Mohan A, Wei H, Miano JM, Florio VA, Gao P, Korshunov VA, Berk

BC, Yan C.*

Role of nuclear Ca2+/calmodulin-stimulated phosphodiesterase 1A in vascular smooth muscle

cell growth and survival. Circ.Res., 98:777-784, 2006.

61. Miano JM, Fishing for function in zebrafish. Circ.Res., 98:723-726, 2006.

62. Miano JM,* Georger MA, Rich A, de Mesy Bentley KL. Ultrastructure of zebrafish dorsal aortic cells.

Zebrafish, 3:455-463, 2006.

63. Miano JM,* and Berk BC. HDAC7 supports vascular integrity (Ed). Nat.Med. 12:997-998, 2006.

64. Miano JM,* Long XC, Fujiwara K. Serum response factor: master regulator of the actin cytoskeleton and

contractile apparatus. Am.J.Physiol. Cell Physiol., 292:C70-C81, 2007.

65. Chow N, Bell RD, Deane R, Streb JW, Guo H, Chen J, Rubio A, Brooks A, Van Nostrand W, Miano JM,*

Zlokovic BV.* Serum response factor and myocardin mediate arterial hypercontractility and cerebral blood

flow dysregulation in Alzheimer’s phenotype. Proc.Natl.Acad.Sci., USA., 104:823-828, 2007. (*Co-senior

authorship).

66. Long X and Miano J.M.* Remote control of gene expression. J.Biol.Chem., 282:15941-15945, 2007.

67. Long X, Creemers EE, Wang DZ, Olson EN, Miano JM.* Myocardin is a bifunctional switch for smooth

versus skeletal muscle differentiation. Proc.Natl.Acad.Sci., USA., 104:16570-16575, 2007.

68. Davis JL, Long X, Georger MA, Scott IC, Rich A, Miano JM.* Expression and comparative genomics of two

serum response factor genes in zebrafish. Int.J.Dev.Biol., 52:389-396, 2008.

69. Long X, Bell RD, Gerthoffer WT, Zlokovic BV, Miano JM.*

Myocardin is sufficient for a smooth muscle

cell-like contractile phenotype. Arterioscler.Thromb.Vasc.Biol., 28:1505-1510, 2008.

70. Miano JM. SRF’ing the actin cytoskeleton with no destrin. Physiol. Genomics., 34:6-8, 2008.

71. Miano JM. Deck of CArGs. Circ.Res., 103:13-15, 2008.

72. Bell RD, Deane R, Chow N, Long X, Sagare A, Streb JW, Guo H, Rubio A, Van Nostrand W,

Miano JM,* Zlokovic BV.

* SRF and myocardin repress LRP-mediated amyloid-beta clearance in brain

vascular smooth muscle cells via SREBP2. Nat. Cell Biol., 11(2):143-153, 2009. Editorialized by Dotti and

Strooder, Nat.Cell Biol. 11:114-16, 2009.


September 13, 2013

73. Cordes KR, Sheehy NT, White M, Berry E, Morton SU, Muth AN, Lee T-H, Miano JM, Ivey KN, Srivastava

D.*

miR-145 and miR-143 regulate smooth muscle cell fate and plasticity. Nature, 460:705-710, 2009.

Editorialized in Cell, 138:813, 2009 and by Parmacek M.S., J.Clin.Invest. 119:2526-28, 2009.

74. Lee T-H, Chen J, Miano JM.*

Functional characterization of a putative serine carboxypeptidase in vascular

smooth muscle cells. Circ.Res, 105:271-278, 2009.

75. Sun Q, Taurin S, Sethakorn N, Long X, Imamura M, Wang DZ, Zimmer

WE, Dulin

NO, Miano J.M.

*

Myocardin-Dependent Activation of the CArG Box-Rich Smooth Muscle Gamma Actin Gene: Preferential

Utilization of a Single CArG Element through Functional Association with the NKX3.1 Homeodomain

Protein. J.Biol.Chem. 284:32582-32590, 2009.

76. Long X, Tharp DL, Georger MA, Slivano OJ, Lee MY, Wamhoff BR, Bowles DK, Miano JM.*

The smooth muscle cell-restricted KCNMB1 ion channel subunit is a direct transcriptional target of serum

response factor and myocardin. J.Biol.Chem. 284:33671-682, 2009.

77. Miano JM. What is truth? Standards of scientific integrity in American Heart Association journals.

Arterioscler.Thromb.Vasc.Biol. 30:1-4, 2010.

78. Albinsson S, Suarez Y, Miano JM, Sessa WC.* miRNAs are necessary for vascular smooth muscle growth

and differentiation during development. Arterioscler.Thromb.Vasc.Biol. 30:1118-26, 2010.

79. Imamura M, Long X, Nanda V, Miano JM.*

Expression and functional activity of four myocardin isoforms.

Gene. 464:1-10, 2010.

80. Miano JM. Role of serum response factor in the pathogenesis of disease. Lab. Invest. 90:1274-84, 2010.

81. Miano JM. Smooth muscle cell differentiation – 2010. Journal of Biomedical Research. 24(3):169-180, 2010.

82. Miano JM. Dicing up microRNA gene expression profiles in normal and neoplastic smooth muscle cells.

Am.J.Pathol. 177:541-543, 2010.

83. Sullivan AL, Benner C, Heinz S, Huang W, Xie L, Miano JM, Glass CK.*

SRF utilizes distinct

promoter and enhancer-based mechanisms to regulate cytoskeletal gene expression in macrophages.

Mol.Cell.Biol. 31:861-875, 2011.

84. Xie W-B, Li Z, Miano JM, Long X, Chen S-Y.* Smad3-mediated myocardin silencing: a novel

mechanism governing the initiation of smooth muscle differentiation. J.Biol.Chem. 286:15050-57, 2011.

85. Streb JW, Long X, Lee T-H, Sun Q, Kitchen CM, Georger MA, Blaner WS, Carr DW, Gelman IH, Miano

JM.*

Retinoid-induced expression and activity of an immediate early tumor suppressor gene in vascular

smooth muscle cells. PLoS One. 6(4):e18538, 2011.

86. Albinsson S, Skoura A, Yu J, DiLorenzo A, Fernández-Hernando C, Offermanns S, Miano JM, Sessa WC.*

Smooth muscle miRNAs are critical for post-natal regulation of blood pressure and vascular function. PLoS

One. Apr 22;6(4):e18869, 2011.


September 13, 2013

87. Park C, Hennig GW, Sanders KM, Cho JH, Hatton WJ, Redelman D, Park JK, Ward SM, Miano JM, Yan W,

Ro S.* SRF-dependent microRNAs regulate gastrointestinal smooth muscle cell phenotypes.

Gastroenterology. 141:164-175, 2011.

88. Long X* and Miano JM.

* TGFβ1 utilizes distinct pathways for the transcriptional activation of microRNA

143/145 in human coronary artery smooth muscle cells. J.Biol.Chem. 286:30119-30129, 2011.

89. Benson CC, Long X, Zhou Q, Miano JM.*

Identifying functional single nucleotide polymorphisms in the

human CArGome. Physiological Genomics. 43:1038-1048, 2011.

90. Long X, Slivano OJ, Cowan SL, Georger MA, Lee T-H, Miano JM.* Smooth muscle calponin: an

unconventional CArG-dependent gene that antagonizes neointimal formation. Arterioscler.Thromb.Vasc.Biol.

31:2172-2180, 2011. (Featured on cover of journal).

91. Miano JM and Small E.M. MicroRNA133a: a new variable in vascular smooth muscle cell phenotypic

switching. Circ.Res. 109:825-827, 2011.

92. Nanda V and Miano JM.* Leiomodin 1: a new serum response factor-dependent target gene expressed

preferentially in differentiated smooth muscle cells. J.Biol.Chem. 287:2459-2467, 2012.

93. Chen J, Yuan K, Mao X, Miano JM, Wu H, Chen Y.* Serum response factor regulates bone formation via

IGF-1 and Runx2 signals. J. Bone Miner.Res. 27:1659-68, 2012.

94. Nicholson TM, Ricke EA, Marker P, Miano JM, Mayer R, Timms B, vom Saal F, Wood R, Ricke WA.

*

Testosterone and estradiol-17ß induced new glandular prostatic growth, bladder outlet obstruction, and voiding

dysfunction in male mice. Endocrinology. 153:5556-65, 2012.

95. Miano JM. Vascular smooth muscle cell phenotypic adaptation; Chapter 94. In Muscle: Fundamental Biology

and Mechanisms of Disease. Eds., Hill, J.A. and Olson, E.N. Academic Press, 2012.

96. Davis J, Maillet M, Miano JM, Molkentin JD.* Lost in transgenesis: A users guide for genetically

manipulating the mouse in cardiac research. Circ. Res. 111:761-777, 2012.

97. Kitchen CM, Cowan SL, Long X, Miano JM.* Expression and promoter analysis of a highly restricted integrin

alpha gene in vascular smooth muscle. Gene. 513:82-89, 2013.

98. Nguyen AT, Gomez D, Bell RD, Campbell JH, Clowes AW, Gabbiani G, Giachelli CM, Parmacek MS,

Raines EW, Rusch NJ, Speer MY, Sturek M, Thyberg J, Towler DA, Weiser-Evans MC, Yan C, Miano

JM, Owens GK. Smooth muscle cell plasticity: fact or fiction? Circ. Res. 112:17-22, 2013.

99. Imamura M,* Sugino Y, Long X, Slivano OJ, Yoshimura N, Miano JM. Myocardin and

MicroRNA-1 modulate bladder activity through connexin 43 expression during post-natal development.

J.Cell.Physiol. 228:1819-26, 2013.

100. Long X, Cowan SL, Miano JM. Mitogen-activated protein kinase 14 is a novel negative regulatory

switch for the vascular smooth muscle cell contractile gene program. Arterioscler.Thromb.Vasc.Biol., 33:378-

386, 2013.

101.Shi G, Field DJ, Long X, Mickelsen D, Ko K-a, Korshunov VA, Miano JM, Morrell CN.* Platelet factor 4

mediates vascular smooth muscle cell injury responses. Blood. 121:4417-4427, 2013.

http://www.ncbi.nlm.nih.gov/pubmed/23175675

http://www.ncbi.nlm.nih.gov/pubmed/23175675


September 13, 2013

102. Bell RD, Long X, Lin M, Cowan SL, Nanda V, Zhou Q, Zheng D, Miano JM.* Identification and

function of SENCR, a novel long non-coding RNA enriched in human vascular cells. In preparation.

103. Long X and Miano JM. Myocardin: new therapeutic agent in vascular disease?

Arterioscler.Thromb.Vasc.Biol. 33:2284-2285, 2013.

104.


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XX. Selected Abstracts

1. Starzec, J.J., Berger, D.F., Corrao, R.M. and Miano JM, Serum cholesterol levels and daily duration of

swimming stress in rats. Eastern Psychological Association, Boston, MA, 1984 (poster).

2. Mettler, N.R., Miano JM,, Wang, W.H. and Kikkawa, Y. Secretory factors from phagocytosing alveolar

macrophages depresses cytochrome P-450 in isolated hepatocytes. Federation Of American Societies Of

Experimental Biology (FASEB) J. 3:A1601, 1988 (poster).

3. Miano JM,, Starzec, J.J. and Berger, D.F. The effects of voluntary exercise on diet-induced alterations in

blood lipids and aortic histopathology in the rate. Eastern Psychological Association, Boston, MA, 1989

(poster).

4. Miano JM,, Tota, R.R., Vlasic, N. and Stemerman, M.B. Early induction of c-myc following balloon injury

to the rat aorta. Fed. of Am. Soc. Exp. Biol. (FASEB) J. 5:A480, 1990 (oral presentation).

5. Tota, R.R., Vlasic, N., Miano JM, and Stemerman, M.B. Vascular smooth muscle cell proto-oncogene

induction by physical manipulation. Arteriosclerosis 10:844a, 1990.

6. Miano JM,, Vlasic, N., Tota, R.R., O'Banion, M.K., Young, D.A. and Stemerman, M.B. Immediate early gene

(IEG) activation in aortic rat smooth muscle cells following vascular injury: Relationship to in vivo growth

factor expression and DNA synthesis. Circulation 84:II,342, 1991 (oral presentation).

7. Tota, R.R., Miano JM,, Vlasic, N. and Stemerman, M.B. Role of immediate early genes (IEG) in autocrine

growth of rat aortic smooth muscle cells (SMC) in vivo following vascular injury. J. Cell. Biochem. 16A:13,

1992 (poster).

8. Giachelli, C.M., Miano JM,, Olson, E.N., Firulli, A.B., Hara, P.S., Soderling, S. and Schwartz, S.M.

Homeobox gene expression distinguishes human fetal and adult aortic smooth muscle cells in culture.

Federation of American Societies Of Experimental Biology (FASEB) Journal. 8:A264, 1994 (poster).

9. Miano JM,, Cserjesi, P., Li, L., Ligon, K.L. and Olson, E.N. Specificity of smooth muscle cell markers in

adult and developing mouse tissues. American Heart Association Meeting, November, 1994.(poster).

10. Miano JM,, Topouzis, S., Majesky, M.W. and Olson, E.N. Smooth muscle cells express functional retinoid

receptors and are negatively growth regulated by all-trans retinoic acid. Keystone Symposium, February, 1996

(poster).

11. Weihrauch, D., Chilian, W.M., Tessmer, J., Miano JM,, and Warltier, D.C. Intermittent myocardial ischemia

results in the expression of mitogens in the cardiac interstitium. American Heart Association Meeting,

November, 1996 (poster).

12. Broeckel, U., Jiang, J., Tonellato, P., Feroah, T., Jacob, H.J. and Miano JM, Assessing the spatial relationship

between functional units of genes as a tool for defining genes from genomic sequence with bioinformatics and

bioassays. Cold Spring Harbor Genome Mapping and Sequencing Meeting. May 14-18, 1997 (poster)


September 13, 2013

13. Miano JM,, Roloff, L.A., Piantedose, R., Blaner, W.S., and Kipshidze, N.N. Systemic administration of

retinoic acid reduces intimal thickening following balloon injury in a rat model. Transcatheter

Cardiovascular Therapeutics IX, Washington, DC, September 24-26, 1997 (Poster).

14. Konorev, E.A., Miano JM,, and Kalyanaraman, B. Bicarbonate enhances doxorubicin-induced myocardial

damage via a superoxide radical-dependent mechanism. AHA Meeting, November, 1998 (Poster).

15. Foschi, M., Barboni, E., Miano JM,, Vandewalle, W., LaVilla, G., Gentilini, P., Franchi, F. Adenoviral-

mediated gene delivery targeted to hepatic stellate cells. Hepatology. 28:520A, 1998 (Poster).

16. Miano JM, Transcriptional control of smooth muscle calponin. Federation of American

societies of experimental biology (FASEB), April 18, 1999 (oral).

17. Miano JM,, Foschi, M., Friesel, R., and Chada, S. Vascular smooth muscle cell-specific gene targeting with

the SM22 promoter. SCVIR, March 25, 2000.

18. Frame, M.D., Miano JM,, Rivers, R.J. Technique for targeting adenoviral constructs to microcirculatory

vascular smooth muscle, in vivo. FASEB, March, 2001.

19. Streb, J.W., Chen, J. Miano JM, Novel expression of a tumor suppressor gene in vascular smooth muscle cells

following retinoid administration. ATVB, May 11-13, 2001. (*NAVBO’s Servier New Investigator Award to

Jeffrey Streb*)

20. Chen, J., Streb, J.W. Miano JM, Cloning of a novel retinoid-response gene in vascular smooth muscle cells.

ATVB, May 11-13, 2001.

21. Miano JM,, Kitchen, C.M., Chen, J., Wang, D-Z., Olson, E.N. Myocardin is expressed in adult aorta and

activates a smooth muscle cell differentiation program. ATVB/NAVBO, April 6, 2002 (oral).

22. Streb, J.W., Georger, M.A., Miano JM, Retinoids activate cyclic AMP response element binding protein in

vascular smooth muscle cells via protein kinase A. ATVB/NAVBO, April 6, 2002.

23. Georger, M.A., Kitchen, C.M., Miano JM, Human smooth muscle calponin protein is tightly restricted to

smooth muscle cell lineages in transgenic mice. ATVB/NAVBO, April 7, 2002.

24. Kitchen, C.M., Chen, J., Streb, J.W., Miano JM, VISTA and PIP analyses reveal unconventional regulatory

domains in a vascular smooth muscle cell-restricted, retinoid-inducible integrin subunit. ATVB/NAVBO,

April 7, 2002.

25. Miano JM, Mining BACs for regulatory elements controlling human smooth muscle calponin expression in

transgenic mice. Cold Spring Harbor LXVII Symposium on the Cardiovascular System, May 30, 2002.

26. Sun, Q., Chen, G., Streb, J.W., Yang, Y., Stoeckert, C., Miano JM, Defining the mammalian CArGome.

Cold Spring Harbor Laboratory, Systems Biology: Global Regulation of Gene Expression. March 18, 2005.

27. Chen, G.G., Miano JM,, Jensen, S.T., Stoeckert, C. Identify functional regulons by integrating heterogeneous

data with Bayesian hierarchical modeling. Systems Biology: Global Regulation of Gene Expression. March

24, 2006.


September 13, 2013

28. Wang, L., Miller, C., Rao, M.H., Miano JM,, Mackman, N., Taubman, M.B. Vascular smooth muscle-

derived tissue factor is critical for arterial thrombosis after Fecl3 injury. ATVB 27:E47-E48, 2007 (poster).

29. Lee, T.H. and Miano JM, Functional analysis of a novel serine carboxypeptidase. ATVB Annual Conference.

April 18, 2008 (poster).

30. Long, X., Hyu, C., Miano JM,, Chen, Y. Mutual regulation of Runx2 and Myocardin during oxidative stress-

induced smooth muscle cell phenotypic modulation. ATVB Annual Conference. April 17, 2008 (poster).

31. Long, X., Bell, R.D., Zlokovic, B.V., Miano JM, Initial characterization of the Myocardin promoter and its

regulation by hypoxia. ATVB Annual Conference. April 18, 2008 (poster).

32. Long, X., Bell, R.D., Zlokovic, B.V., Miano JM, Myocardin is sufficient for the smooth muscle cell

contractile phenotype. ATVB Annual Conference. April 18, 2008 (oral, Long).

33. Garvey, S.M., Lee, M.Y., Miano JM,, Wamhoff, B.R. Expression analysis of microRNAs associated with

vascular smooth muscle cell phenotypic modulation. European Federation of Physiology. March 29, 2010

(Poster).

34. Long, X and Miano, JM. MicroRNAs and SMC Phenotype. Acta Physiologica 198:SUPPL 677, 5, 2010.

35. Bell, R.D., Lin, M., Cowen, S., Nanda, V., Long, X., Zheng, D., Miano JM, Cold Spring Harbor Laboratory

Meeting on Regulatory & Non-Coding RNAs. August 31, 2012 (Oral, Bell).


September 13, 2013

XXI. Patents

1. Olson, E.N., Li, L., and Miano JM, “Smooth muscle 22 promoter, gene transfer vectors containing the same,

and method of use of the same to target gene expression in arterial smooth muscle cells.” Patents Issued

November 17, 1998 (US Patent No. 5,837,534) and January 18, 2000 (US Patent No. 6,015,711). Licensed to

AmGen.

joseph michael miano, ph.d. - university of...

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