VOLUME 46, NUMBER 6

JOURNAL OF BACTERIOLOGYOfficial Organ of the Society of American Bacteriologists

Founded in 1916DEVOTED TO THE ADVANCEMENT AND DISSEMINATION OF KNOWL-EDGE IN REGARD TO THE BACTERIA -AND OTHER MICRO-ORGANISMS

Publication Board of the SocietyChairman, C.-E. A. WINSLOW

Yale Medical School,- New Haven, Conn.

R. E. BUCHANAN H. J. CONNBARNETT COHEN A. P. HITCHENS

The President and Secretary of the Society, ex officio.

Journal of BacteriologyEditor

C.-E. A. WINSLOWAssociate Editors

L. D. FOTHERGILL GEORGES KNAYSI STUARTMUDD J. H. MUELLERM. J. PICKETT J. M. SHERMAN

Advisory EditorsG. P. BERRY J. F. ENDERS C. B. LIPMAN T. M. RIVERSR. S. BREED W. A. HAGAN A. G. LOCHHEAD M. J. ROSENAUR. E. BUCHANAN B. W. HAMMER K. F. MEYER G. H. SMITHE. J. CAMERON M. W. JENNISON G. B. REED C. V. SMYTHEP. F. CLARK S. A. KOSER L. F. RETTGER W. H. TALIAFERRO

Contentsfor DecemberArthur Trautwein Henrici ............................ ! ...... 489Some characteristics of green-fluorescent pigment-producing bac-

teria. W. A. SELEEN AND C. . STARK.491A simple technique for the detection of melibiose-fermenting yeasts

LYNFERD J. WICKERHAM.501On the enrichment and purification of chromogenic spore-forming

anaerobic bacteria. L. S. MCCLUNG..507The accelerating effect of sublethal heat on spore germination in

mesophilic aerobic bacteria. FRED R. EVANS AND HAROLDR. CURRAN.513

Machine for shell freezing small volumes of biological preparations.L. D. ANTOINE AND M. V.HARGETT..O . 525

Effect of long ultraviolet and short visible radiation (3500 to 4900A)on escherichia coli. ALEXANDER HOLLAENDER.531

Penicillin. Its use in media for the isolation of H. Influenzae fromlaryngeal cultures in obstructive laryngitis. LILLIAN Bux-BAUM AND NICHOLAS F. FIEGOLI .......................... 543

Bacteriologic procedures in sanitary air analysis. With specialreference to air disinfection. WV. F. WELLS................. 549

Growth requirements of clostridium tetani. II. Factors exhaustedby growth of the oiganism. ROBERT E. FEENEY, J. HOWARDMiUELLER AND PAULINE A. MNILLER........................ 559

Growth requirements of clostridium tetani. III. A "Syrnthetic"Mledium. ROBERT E. FEENEY, J. HOWARD MUELLER ANDPAULINE A. MILLER............. 563

Proceedingfs of local branches of the societv of American bacteriolo-gists.................. 573

Index.................. 579

(In writing to advertisers, please mention the journal-it helps.)

2 JOURNAL OF BACTERIOLOGY

INDEX

Acetone, ethyl alcohol, butyl alcohol,chloroform, ether, and urethane, Ananalysis of the antagonistic and thesynergistic action of, on sulfanilamideinhibitions. Abstract, 110

Acidity and hydrogen-ion concentrationrelationships among the lactic acidbacteria. Abstract, 221

Actinomyces coelicolor and A. violaceus-ruber, The pigment production of, 133

Actinomycetes, The nomenclature and clas-sification of the, 337

Actobacter, Motility in the genus, 394Aerobacter cloacae, Studies on strains of,

responsible for acute illness amongworkers using low-grade stained cotton.Abstract, 217

Aerobic mesophilic bacteria from soil,Cellulose decomposition by. I. Isola-tion and description of organisms, 273II. Biochemical studies on filter paperand cellulose preparations, 281

. III. The effect of lignin, 291Agar contamination, Studies on, as affecting

the sterilization of culture media, 269-,Recovery of, from used media, 396Agglutination and precipitin reactions, A

comparison of the value of the, in theserological typing of group A strepto-cocci, 117

Agglutinins in swine blood serum followingthe feeding of bovine fetal and pla-cental tissue infected with Brucellaabortus. Abstract. (No abstract), 223

Air analysis, sanitary, Bacteriologic pro-cedures in, with special reference to airdisinfection, 549

- disinfection, Bacteriologic procedures insanitary air analysis, with specialrefer-ence to, 549

Aisenberg, Myron S., and Grubb, ThomasC., 311

Alderman, Ilo, and Aronson, Joseph D.,261; Abstract, 218

Allantoin, Streptococcus allantoicus andthe fermentation of, 251

Anaerobes, Fibrinolysins from gas gangrene,475

Anaerobic bacteria, On the enrichment andpurification of chromogenic spore-form-ing, 507

-, Recent developments concerning the, andtheir activities, with particular refer-ence to the tetanus and gangrene or-ganisms. Abstract, 577

-, Use of dried tissue in beef heart mediumfor, 215

Anaerobic conditions, An improved technicfor growing microorganisms under, 373

- culture tube, An, for determining C02/H,ratios of coliform bacteria, 89

- microorganisms, An improved technic forthe cultivation of. Abstract, 314

Anderson, A. A., 396Andersen, Thomas F. Abstract, 110-, Luria, S. E., and Delbruck, M., 57-, Mudd, Stuart, and Heinmets, Ferdi-

nand, 205, Mudd, Stuart, andPolevitzky, Katherine,

15Anigstein, Ludwik, Bader, Madero N., and

Young, Gerald. Abstract, 218Antibiotic substance (penatin), The action

of, on bacteriophage. Abstract, 110- substances elaborated by an A8pergillus

flavu.s strain and by an unclassifiedmold, Note on. Abstract, 109

- substances, The nature and mode ofaction of. Abstract. (No abstract),222

Antibodies, The occurrence of natural,against certain yeast-like fungi. Ab-stract, 486

Antisera for Paramecium aurelia. A tech-nique for the production of. Abstract,576

Antoine, L. D., and Hargett, M. V., 525Aronson, Joseph D., and Alderman, Ilo,

261; Abstract, 218Arsenic, The effect of fever on the distribu-

tion of, in rabbits following the intra-venous administration of marphasen.Abstract. (No abstract), 574

Aspergillus flavus strain, Note on anti-biotic substances elaborated by an,and by an unclassified mold. Ab-stract, 109

Azotobacter, A Study of the relationship ofgrowth energy source to respiration in.Abstract, 485

-, Flagellation of. Abstract. (No ab-stract), 574

, Measuring the growth of. Abstract, 485579

Bacillus brevis, Formation of tyrothricinin submerged cultures of, 83

Bacterial morphology as shown by theelectron microscope. VI. Capsule,cell-wall and inner protoplasm of pneu-mococcus, type III, 205

Bactericidins, Natural, in the plasma of thedomestic fowl. Abstract, 575

Bacteriophage, The action of an antibioticsubstance (penatin) on. Abstract, 110

Bacterium columbensis Castellani, The iden-tity of, 79

Bader, Madero N., Aningstein, Ludwik,and Young, Gerald. Abstract, 218

Bagg, Josephine V., and Pederson, Carl S.Abstract, 221

Baird, Elizabeth A., Wood, A. J., andKeeping, Frances E., 106

Barker, H. A., 251Beal, Grace A., and Schuhardt, V. T.

Abstract, 219Beef heart medium, Use of dried tissue in,

for anaerobic bacteria, 215Bellamy, W. D., Campbell, J. J. R., and

Gunsalus, I. C. Abstract, 573- and Gunsalus, I. C. Abstract, 573Bergner, S., Kligler, I. J., and Grossowicz,

N., 399Bernheim, Frederick, and Bernheim, Mary

L. C., 225Bernheim, Mary L. C., and Bernheim,

Frederick, 225Biochemical studies on filter paper and

cellulose preparations. Cellulose de-composition by aerobic mesophilicbacteria from soil. II, 281

Biological preparations, Machine for shellfreezing small volumes of, 525

-research, Application of the electronmicroscope to. Abstract, 576

Blair, John E. Abstract, 313Blevins, Anne, MacNeal, Ward J., and

Pacis, Marcello. Abstract, 111Blood, Cultivation of Brucella from the, 333Boak, R. A., Stokinger, H. E., Dorn, F. L.,

and Carpenter, C. M. Abstract. (Noabstract), 574

Bohls, S. W., and Irons, J. V. Abstract, 218Borman, Earle K., and Wheeler, Kenneth

M., 481Braun, Armin C., and White, Philip R.

Abstract, 109Braun, Werner. Abstract, 222Breed, Robert S. Abstract, 221-. Abstract. (No abstract), 574

Brewer, John H., 395Broadhurst, Jean, MacLean, Estelle, and

Taylor, Inez. Abstract, 110Brooks, Robert F. Abstract, 574Brown, Helen J., Reed, G. B., and Orr,

J. H., 475Brucella abortus, Agglutinins in swine blood

serum following the feeding of bovinefetal and placental tissue infected with.Abstract. (No abstract), 223

-abortus, Studies on the nature and controlof dissociation in. Abstract, 222

-, Cultivation of, from the blood, 333-, Preliminary observations of growth of

selected strains of, in the McCulloughand Dick mineral base medium. Ab-stract, 219

- spp., Variation of, with reference to thebacteriostatic action of dyes. Ab-stract, 219

Bucca, Matthew A., 151Burkholder, W. H. Abstract. (No ab-

stract), 574Butler University, Bacteriology at. Ab-

stract, 576Butyric acid bacteria from olives, Charac-

teristics of, 315Buxbaum, Lillian, and Fiegoli, Nicholas

F., 543B-vitamin, The, requirements of the pro-

pionibacteria, 99

Caminita, B. H., Schneiter, R., Kolb, R. W.,and Neal, P. A. Abstract, 217

Campbell, J. J. R., Bellamy, W. D., andGunsalus, I. C. Abstract, 573

Cancerous properties, Some evidence onthe etiology of, as exemplified in plantcells. Abstract, 109

Cantor, A., and Schobert, William, 203Caroline, Leona, and Schade, Arthur L., 463Carpenter, C. M., Plack, Jane, and Stoking-

er, H. E. Abstract, 220, Stokinger, H. E., Dorn, F. L., and Boak,R. A. Abstract. (No abstract), 574

Casein, Digestion of, by staphylococci onmilk agar containing serum, 392

Cellulose decomposition by aerobic meso-philic bacteria from soil. I. Isolationand description of organisms, 273

-. II. Biochemical studies on filter paperand cellulose preparations, 281

. III. The effect of lignin, 291

580 INDEX

INDEX5

Central New York Branch, S. A. B., Pro-ceedings, 220, 573

Centrifuge, Separating soil algae fromother organisms by means of the.Abstract, 222

Chlormercuri carvacerol, The inhibitoryeffect of on the growth of pathogenicfungi. Abstract, 487

Choriomeningitis, Association of the virusof lymphocytic, with erythrocytes ofinfected animals, 482

Chromogenic spore-forming bacteria. Onthe enrichment and purification of, 507

Clifton, C. E. Abstract. (No abstract),223

Clostridia, Effect of various concentrationsof iron on the production of riboflavinby certain. Abstract, 575

Clostridium acetobutylicum, On the use ofhydrolyzed wheat mash for the enrich-ment of, 214

Clostridium tetani, Growth requirements of.II. Factors exhausted by growth of theorganism, 559III. A "synthetic" medium, 563

Coliform bacteria, An aerobic culture tubefor determining CO2/H2 ratios of, 89

Collins, Carolyn, Sherwood, Noble P., andTanner, William A. Abstract, 485

Conn, Jean E., 133-and Rahn, Otto. Abstract. (No ab-

stract), 221Corynebacterium and Neisseria, Two agar-

less media for the rapid isolation of, 233-, Taxonomy of certain members of the

genus, from animal sources. Abstract,574

Crecelius, H. Gilbert, and Rettger, Leo F., 1Crystal violet, Bacteriostasis by. Ab-

stract. (No abstract), 221-, The use of sodium azide (NaNs) and,

in a selective medium for streptococciand Erysipelothrix rhusiopathiae, 343

Culture media, The coagulation and steri-lization of, 203

Curran, Harold R., and Evans, Fred R., 513

d-Amino acids, Absence of, in the proteinsof virus-induced tumors in rabbits.Abstract. (No abstract), 573

Decomposition of vitamin C by bacteria, 351Delbriuck, M., and Luria, S. E. Abstract,

574, Luria, S. E. and Anderson, T. F., 57

DePauw University, History of bacteriologyat. Abstract, 576

Disinfection and antisepsis, The role ofpH in. Abstract. (No abstract), 221

Dorn, F. L., Stokinger, H. E., Boak, R. A.,and Carpenter, C. M. Abstract. (Noabstract), 574

Doudoroff, M. Abstract. (No abstract),223

Drake, Charles H. Abstract, 486Dyes, Variation of Brucella spp. with refer-

ence to the bacteriostatic action of.Abstract, 219

Dysentery, anti-, agents in embryonatedeggs, Test of. Abstract, 111

-, Bacillary. Abstract, 314, Bacilli, Experimental variation of nico-tinamide requirement of, 239

, bacteriophage, The preparation of apolyvalent, in a dry and stable form.I. Preliminary investigations and gen-eral procedures, 463

Eastern Pennsylvania Chapter, S. A. B.Proceedings, 313

, the New Jersey and the New York CityBranches, S. A. B., Joint meeting ofthe. Proceedings, 109

Eggs, Bacteriology of. Abstract. (No ab-stract), 487

, embryonated, Test of anti-dysenteryagents in. Abstract, 111

Eijkman reactions of Shigella cultures,Further studies on the, 105

Eiman, John A., and Fowler, Harold W.Abstract. (No abstract), 314

Electron microscope, A study of luminousbacterial cells and cytolysates withthe, 167

, Application of the, to biological research.Abstract, 576

-, Bacterial morphology as shown by the.V. Treponema pallidum, T. macro-dentium and T. microdentium, 15

-, Bacterial morphology as shown by the.VI. Capsule, cell-wall and inner pro-toplasm of pneumococcus, type III, 205studies of bacterial viruses, 57

Encephalitis, St. Louis, Persistence of thevirus of, in the central nervous systemof mice for over five months, 113

Enrichment and purification of chromogenicspore-forming anaerobic bacteria, Onthe, 507

Ervin, Robert F., and Reyniers, James A.Abstract, 577

581

INDEX

Erysipelothrix rhusiopathiae, The use ofsodium azide (NaNs) and crystal violetin a selective medium for streptococciand, 343

Erythrocytes of infected animals, Associa-tion of the virus of lymphocytic chorio-meningitis with, 482

Escherichia coli, Effect of long ultravioletand short visible radiation (3500 to4900 A) on, 531

Evans, Alice C. Abstract. (No abstract),217

Evans, Fred R., and Curran, Harold R., 513Eyring, Henry B., Johnson, Frank H., and

Kearns, Walter. Abstract, 110

Feeney, Robert E., Mueller, J. Howardand Miller, Pauline A., 559, 563

Felsen, Joseph. Abstract, 314Fever, The effect of, on the distribution of

arsenic in rabbits following the intra-venous administration of marphasen.Abstract. (No abstract), 574

Fibrinolysins from gas gangrene anaerobes,475

Fiegoli, Nicholas F., and Buxbaum, Lillian,543

Filters, The use of "Pyrex" brand fritted,in bacteriological work, 312

Fisk, Roy T., and Mordvin, Olga E., 392Flagellation of Azotobacter. Abstract. (No

abstract), 574, Types of, found among bacteria. Ab-

stract. (No abstract), 574Foley, G. E., and Wheeler, S. M., 391Foltz, V. D. Abstract. (No abstract),

487Food and dairy plants, The sanitation of

pyrex glass tubing used to replace metaltubing in. Abstract. (No abstract),220

Foster, J. W., Stokes, J. L., and Woodward,C. R., Jr. Abstract, 109

- and Wilker, Bernard L., 377- and Woodruff, H. Boyd, 187

, Woodruff, H. B., and McDaniel, L. E.,421

Fowler, Harold W., and Eiman, John A.Abstract. (No abstract), 314

Fuller, W. H., and Norman, A. G., 273,281, 291

Fulton, MacDonald, 79- and Harrison, Preston E., 365

Fungi, pathogenic, The inhibitory effectof chlormercuri carvacrol on the growthof. Abstract, 487

Gaines, Sidney, and Stahly, Grant L., 441Gainey, P. L. Abstract, 485Georgi, Carl E. Abstract, 487Germicides, The effect of, on the viability

and on the respiratory enzyme activityof, 151

Gerstung, Ruth B., and Landy, Maurice.Abstract. (No abstract), 217

Giese, Arthur C., 323Gililland, J. Richard, and Vaughan, Reese

H., 315Glucose and maltose, Production of acid

from, by type I meningococci in achemically defined medium. Abstract.(No abstract), 221

-, Studies on the role of niacin and thia-mine in the metabolism of, by Staphy-lococcus aureus, 399

Gonococcus, A chemically defined mediumfor the cultivation of the. Abstract,220

- The effect of germicides on the viabilityand on the respiratory enzyme activityof, 151

Green-fluorescent pigment-producing bac-teria, Some characteristics of, 491

Grossowicz, N., Kligler, I. J., and Bergner,S., 399

Growth energy source, A study of the rela-tionship of, to respiration in Azoto-bacter. Abstract, 485

-of bacterial viruses and of their host,Dissociation of the, by means of tem-peratures above optimum. Abstract,575

Growth requirements of Clostridium tetani.II. Factors exhausted by growth ofthe organism, 559III. A "synthetic" medium, 563

Grubb, Thomas C., and Aisenberg, MyronS., 311

Gunsalus, I. C. and Bellamy, W. D. Ab-stract. 573Campbell, J. J. R., and Bellamy, W. D.Abstract, 573

Habbu, M. K., and Sokhey, S. S., 25, 33Hargett, M. V., and Antoine, L. D., 525Haring, C. M. Abstract. (No abstract),

223Harris, John 0. Abstract, 485

or-002

INDEX

Harrison, Preston E., and Fulton, Mac-Donald, 365

Heat, The accelerating effect of sub-lethal,on spore germination in mesophilicaerobic bacteria, 613

Heat-treated bacteria, Further studies ofthe effect of the medium on the appar-ent survival of. Abstract, 486

Heinmets, Ferdinand, Mudd, Stuart, andAnderson, Thomas F., 205

Henle, Gertrude, and Henle, Werner. Ab-stract, 314

Henle, Werner, and Henle, Gertrude. Ab-stract, 314

H. influenzae, Penicillin-its use in mediafor the isolation of, from laryngealcultures in obstructive laryngitis, 543

Henrici, Arthur Trautwein, 1889-1943, 489- and Waksman, Selman A., 337Hofer, AlvinW. Abstract. (No abstract),

574Hoffmann, C. E. Abstract. (No abstract),

221Hollaender, Alexander, 531Hucker, G. J. Abstract. (No abstract),

220Human infection, A note on non-group-A

streptococci associated with, 391Hunter, Charles A. Abstract, 487Hydrogen ion concentrations, Optimum

and limiting, for the growth of theplague bacillus in broth, 33

Immune serum, Protective effect of separateinoculation of spotted fever virus and,by intradermal route. Abstract, 218

Immunization, War-time. Abstract, 577Indiana Branch, S. A. B., Proceedings, 574- University, Notes concerning the his-

tory of bacteriology at. Abstract, 576Influenza A virus, Factors influencing the

propagation of, in the developing chickembryo. Abstract, 314

, Epidemic, vaccine and antiserum. Ab-stract, 577

-, Preparedness for defense against.Abstract, 111

Intestinal flora, The, of the guinea pig, 1Iron, Effect of various concentrations of,

on -the production of riboflavin bycertain clostridia. Abstract, 575

Irons, J. V., and Bohls, S. W. Abstract,218

Isolation and description of organisms.Cellulose decomposition by aerobicmesophilic bacteria from soil. I, 273

Jamieson, W. A. Abstract, 577Johnson, Frank H., Eyring, Henry B., and

Kearns, Walter. Abstract, 110, Zworykin, Nina and Warren, George, 167

Kearns, Walter, Johnson, Frank H., andEyring, Henry B. Abstract, 110

Keeping, Frances E., Wood, A. J., andBaird, Elizabeth A., 106

Kerby, Grace P., and Wise, Bowman, 333Kligler, I. J., Grossowicz, N., and Bergner,

S., 399Knaysi, Georges, 451Kocholaty, Walter. Abstract, 313Kolb, R. W., Caminita, B. H., Schneiter,

R., and Neal, P. A. Abstract, 217Koser, Stewart A., and Wright, Marjory

H., 239Krumwiede, Elma, 117

Lactic acid bacteria, Acidity and hydrogen-ion concentration relationships amongthe. Abstract, 221

Landy, Maurice, and Gerstung, Ruth B.Abstract. (No abstract), 217

Laryngitis, Penicillin-its use in media forthe isolation of H. influenzae fromlaryngeal cultures in obstructive, 543

Leuconostoc mesenteroides, The growth re-quirements of, and preliminary studieson its use as an assay agent for severalmembers of the vitamin B complex, 441

Levin, Marshall, 233Lignin, The effect of. Cellulose decomposi-

tion by aerobic mesophilic bacteria fromsoil. III, 291

Lindegren, Carl C., and Lindegren, Ger-trude, 405

Lindegren, Gertrude, and Lindegren, CarlC., 405

Luminous bacteria, Studies on the nutri-tion of dim and bright variants of aspecies of, 323

-bacterial cells and cytolysates, A studyof, with the electron microscope, 167

Luria, S. E. Abstract, 575, and Delbruck, M. Abstract, 574, Delbrutck, M., and Anderson, T. F., 57

583

INDEX

MacLean, Estelle, Broadhurst, Jean, andTaylor, Inez. Abstract, 110

MacNeal, Ward J., Blevins, Anne, andPacis, Marcello. Abstract, 111

- and Parker, Ernestine R. Abstract, 111Marphasen, The effect of fever on the dis-

tribution of arsenic in rabbits follow-ing the intravenous administration of.Abstract. (No abstract), 574

McBee, R. H., and Speck, M. L., 89McClung, L. S., 214, 215-. Abstract, 576, 577-and Saunders, Allen. Abstract, 575McCullough and Dick mineral base medium,

Preliminary observations of growth ofselected strains of Brucella in the.Abstract, 219

McDaniel, L. E., Foster, J. W., and Wood-ruff, H. B., 421

Media, meat infusion, Vegetable bacterio-logical'media as substitutes for, 395

, Recovery of agar from used, 396, Studies on agar contamination as affect-ing the sterilization of culture, 269

, Vegetable bacteriological, as substitutesfor meat infusion media, 395

-, Two agar-less, for the rapid isolation ofCorynebacterium and Neisseria, 233

Medium, A "synthetic". Growth require-ments of Clostridium tetani. III, 563

, Further studies of the effect of the, onthe apparent survival of heat-treatedbacteria. Abstract, 486

Melibiose-fermenting yeasts, A simple tech-nique for the detection of, 501

Meningitis, Effective blood and spinal fluidlevels of sulfonamide drugs in the treat-ment of. Abstract. (No abstract), 314

-, The occurrence and bacteriological char-acteristics of S. marcescens from acase of. Abstract, 218

-, The occurrence and bacteriological char-acteristics of S. marcescens from acase of, 261

Meningococci, Preparation of specific poly-saccharide from type I, grown in achemically defined medium. Abstract,221

-, Production of acid from glucose andmaltose by type I, in a chemically de-fined medium. Abstract. (No ab-stract), 221

Menzel, Arthur E. 0., Wintersteiner, 0.,and Rake, Geoffrey. Abstract, 109

Mesophilic aerobic bacteria, The acceler-ating effect of sublethal heat on sporegermination in, 513

Microbial tartrate decomposition. Ab-stract. (No abstract), 223

Microbiological aspects of penicillin. I.Methods of assay, 187

Milk agar containing serum, Digestion ofcasein by staphylococci on, 392

Miller, Pauline A., Feeney, Robert E., andMueller, J. Howard, 559, 563

Missouri Valley Branch, S. A. B. Proceed-ings, 485

Mold, Note on antibiotic substances elabo-rated by an Aspergillus flavua strainand by an unclassified. Abstract, 109

Moore, Donald V., and Thurman, D. C.Abstract, 220

Mordvin, Olga E., and Fisk, Roy T., 392Morton, Harry E., 312, 373; Abstract, 314Mudd, Stuart, Heinmets, Ferdinand, and

Anderson, Thomas F., 205, Polevitzky, Katherine and Anderson,Thomas F., 15

Mueller, J. Howard, Feeney, Robert E.,and Miller, Pauline A., 559, 563

Naylor, H. B. Abstract. (No abstract),222

Neal, P. A., Caminita, B. H., Schneiter, R.,and Kolb, R. W. Abstract, 217

Nelson, F. E. Abstract, 486Neurospora sitophila, Synthesis of pyri-

doxine by a "pyridoxinless" X-raymutant of. Abstract, 109

New Jersey, The Eastern Pennsylvania,and the New York City Branches,S. A. B., Joint meeting of the. Pro-ceedings, 109

New York City, Joint meeting of the NewJersey, Eastern Pennsylvania, and the,Branches, S. A. B. Proceedings, 109

Newton epidemic, The. Abstract, 487Niacin and thiamine, Studies on the role

of, in the metabolism of glucose byStaphylococcus aureu8, 399

Nicotinamide requirement of dysenterybacilli, Experimental variation of, 239

Nicotinic acid requirements of *certainyeasts, 435

Niven, C. F., Jr., Abstract, 573Norman, A. G., and Fuller, W. H., 273,

281, 291

584

INDEX

Northern-California Hawaiian Branch,S. A. B., Proceedings, 222

Notre Dame, The history of bacteriology atthe University of. Abstract, 577

Nutrition, Studies on the, of dim andbright variants of a species of lumi-nous bacteria, 323

-, The, of group B streptococci. Abstract,573

Nutritional studies on Streptococcus lacti8.I. An unidentified growth factor foundin yeast extract, 369

Organic acids as substrates for streptococci.Abstract, 573

Orr, J. H., Reed, G. B., and Brown, HelenJ., 475

Oxidation of certain sugars and pyruvicacid by a type I avirulent pneumo-coccus, The effect of various substanceson the, 225

Pacis, Marcello, MacNeal, Ward J., andBlevins, Anne. Abstract, 111

Packer, R. Allen, 343Palmer, C. M. Abstract, 576Paramecium aurelia, A technique for the

production of antisera for. Abstract,576

Parker, Ernestine R., and MacNeal, WardJ. Abstract, 111

Pasteurella pesti8, Physiology and nutri-tional requirements of. Abstract.(No abstract), 223

Pederson, Carl S., and Bagg, Josephine V.Abstract, 221

Penatin, the second antibacterial substanceproduced by Penicillium notatum West-ling "77." Abstract, 313

Penicillin inhibition, Turbidimetric studieson. Microbiological aspects of penicil-lin. II, 377

Penicillin- its use in media for the isolationof H. influenzae from laryngeal culturesin obstructive laryngitis, 543

-, Microbiological aspects of. I. Methodsof assay, 187

-, Microbiological aspects of. II. Turbid-imetric studies on penicillin inhibition,377

-, Microbiological aspects of. III. Pro-duction of penicillin in surface culturesof Penicillium notatum, 421

-, Production of, in surface cultures ofPenicillium notatum. Microbiologicalaspects of penicillin. III, 421

, Rapid production of. Abstract. (Noabstract), 223

Penicillium notatum, Production of penicil-lin in surface cultures of. Micro-biological aspects of penicillin. III, 421

- Westling "77," Penatin, the second anti-bacterial substance produced by. Ab-stract, 313

Phytopathogenic bacteria, The gram posi-tive. Abstract. (No abstract), 574

Pigment-producing bacteria, Some char-acteristics of green-fluorescent, 491

Pigment production, The, of Actinomycescoelicolor and A. violaceus-ruber, 133

Plack, Jane, Stokinger, H. E., and Car-penter, C. M. Abstract, 220

Plague bacillus, Optimum and limitinghydrogen ion concentrations for thegrowth of the, in broth, 33

- bacillus, Optimum and limiting tempera-tures for the growth of the, in broth, 25

Plant cells, Some evidence on the etiologyof cancerous properties as exemplifiedin. Abstract, 109

Plasma of the domestic fowl, Natural bac-tericidins in the. Abstract, 575

Pneumococcus, The effect of various sub-stances on the oxidation of certainsugars and pyruvic acid by a type Iavirulent, 225

, type III, Capsule, cell-wall and innerprotoplasm of. Bacterial morphologyas shown by the electron microscope.VI, 205

Polevitzky, Katherine, Mudd, Stuart, andAnderson, Thomas F., 15

Poliomyelitis in Texas, Field and labora-tory studies of. Abstract, 218

- induced by inoculation of tooth pulpcavities, 311

Polysaccharide, Preparation of specific,from type I meningococci grown in achemically defined medium. Abstract,221

Powell, H. M. Abstract, 577Propionibacteria, The B-vitamin require-

ments of the, 99Proteus ammoniae, The characteristics of,

365- hydrophilus, A note on the taxonomy of,

213

585

INDEX

Purdue University, History of bacteriologyin Indiana. Abstract, 576

Pyrex glass tubing, The sanitation of, usedto replace metal tubing in food anddairy plants. Abstract. (No ab-stract), 220

Pyridine analogue of thiamine, Antibac-terial action of a, 483

Pyridoxine, Synthesis of, by a "pyridoxin-less" X-ray mutant of Neuro8porasitophila. Abstract, 109

Radiation (3500 to 4900 A), Effect of longultraviolet and short visible, on Es-cherichia coli, 531

Rahn, Otto, and Conn, Jean E. Abstract.(No abstract), 221

Rake, Geoffrey, Menzel, Arthur E. O.,and Wintersteiner, 0. Abstract, 109

Reed, G. B., Orr, J. H., and Brown, HelenJ., 475

Relapsing fever, Recent observations on.Abstract, 220

Respiration, A study of the relationship ofgrowth energy source to, in Aztobacter.Abstract, 485

Rettger, Leo F., and Crecelius, H. Gilbert, 1- and Young, Raymond M., 351Reyniers, James A. and Ervin, Robert F.

Abstract, 577Riboflavin, Effect of various concentrations

of iron on the production of, by certainclostridia. Abstract. 575

Rogosa, M., 435Rustigian, Robert, and Stuart, C. A., 105

S. marcescens, The occurrence and bac-teriological characteristics of, from acase of meningitis. Abstract, 218

, The occurrence and bacteriological char-acteristics of, from a case of meningitis,261

Salmonella serotypes, Two new, isolatedfrom man, 481

Saunders, Allen, and McClung, L. S. Ab-stract, 575

Schade, Arthur L., and Caroline, Leona, 463Scherp, Henry W. Abstract. (No ab-

stract), 221, 573Schneiter, R., Caminita, B. H., Kolb, R. W.,

and Neal, P. A. Abstract, 217Schnetzler, E. E. Abstract, 575Schobert, William, and Cantor, A., 203

Schuhardt, V. T., and Beal, Grace A. Ab-stract, 219

Seleen, W. A., and Stark, C. N., 491deShazo, Thelma. Abstract. (No ab-

stract, 219Shell freezing small volumes of biological

preparations, Machine for, 525Sherwood, Noble P., Tanner, William A.,

and Collins, Carolyn. Abstract, 485Shigella, A primary division of the genus,

based on the trimethylamine test, 106- cultures, Further studies on the Eijk-

man reactions of, 105Shwartzman, Gregory, 482Silage making, The results of some experi-

ments where salt was added in. Ab-stract. (No abstract), 222

Slavin, Howard B., 113Smith, F. R., 369Sodium azide (NaN,), The use of, and cry-

stal violet in a selective medium forstreptococci and Erysipelothrix rhusio-pathiae, 343

Soil algae, Separating, from other organismsby means of the centrifuge. Abstract,222

Sokhey, S. S., and Habbu, M. K., 25, 33Solid surfaces, The effect of, upon bacterial

activity, 39Sorenson, R. M. Abstract. (No abstract),

486Speck, M. L., and McBee, R. H., 89Spirocheticidal action and reagin titer of

sera of rabbits infected for varyingperiods of time with Treponema paUi-dum, A comparison of the. Abstract,485

Spore germination, The accelerating effectof sublethal heat on, in mesophilicaerobic bacteria, 513

Spotted fever virus, Protective effect ofseparate inoculation of, and immuneserum by intradermal route. Abstract,218

Stahly, Grant L., and Gaines, Sidney, 441Stanier, R. Y., 213Staphylococci, Digestion of casein by, on

milk agar containing serum, 392%

S,Some recent developments in the studyof. Abstract, 313

Staphylococcus aureus, Retention of sul-fonamide resistance and increased p-amino-benzoic acid production in. Ab-stract. (No abstract), 217

586

INDEX5

Stark, C. N., and Seleen, W. A., 491Starkey, Robert L., and Waksman, Selman

A., 489Stokes, J. L., Foster, J. W., and Woodward,

C. R., Jr. Abstract, 109- and Woodward, C. R., Jr., 83Stokinger, H. E., Dorn, F. L., Boak, R. A.,

and Carpenter, C. M. Abstract. (Noabstract), 574

-, Plack, Jane, and Carpenter, C. M.Abstract, 220

Streptococci, A comparison of the value ofthe agglutination and precipitin reac-tions in the serological typing of groupA, 117

-, A note on non-group-A, associated withhuman infection, 391

-and Erysipelothrix rhwiopathiae, The useof sodium azide (NaNa) and crystalviolet in a selective medium for, 343

-, Organic acids as substrates for. Ab-stract, 573

, The nutrition of group B. Abstract, 573Streptococcic bacteriophage: a review.

Abstract. (No abstract), 217Streptococcus allantoicus and the fermenta-

tion of allantoin, 251- fecalis, Growth requirements of, for ty-

rosine decarboxylation. Abstract. 573- lactis. Abstract. (No abstract), 222- lactis. Nutritional studies on. I. An

unidentified growth factor found inyeast extract, 369

Streptothricin, Production and activity of,299

Stuart, C. A., and Rustigian, Robert, 105Sulfa-drugs, A comparison of the action of,

on the growth of a bacterial virus andof its host. Abstract, 574

Sulfanilamide inhibitions, An analysis ofthe anatagonistic and the synergisticaction of acetone, ethyl alcohol, butylalcohol, chloroform, ether, and ure-thane on. Abstract, 110

Sulfonamide drugs in the treatment ofmeningitis, Effective blood and spinalfluid levels of. Abstract. (No ab-stract), 314

- resistance, Retention of, and increasedp-amino-benzoic acid production inStaphylococcus aureus. Abstract. (Noabstract), 217

Sulkin, S. E. Abstract. (No abstract), 220Syphilis, The clinical aspects of. Abstract.

(No abstract), 486

Tanner, William A., Sherwood, Noble P.,and Collins, Carolyn. Abstract, 485

Taxonomic research, Practical values from.Abstract, 221

Taxonomy, A note on the, of Proteus hydro-philus, 213

- of certain members of the genus Coryne-bacterium from animal sources. Ab-stract, 574

Taylor, Inez, Broadhurst, Jean, and Mac-Lean, Estelle. Abstract, 110

Technic, An improved, for growing micro-organisms under anaerobic conditions,373

Temperatures above optimum, Dissociationof the growth of bacterial viruses andoftheirhostby means of. Abstract, 575

-, Optimum and limiting, for the growth ofthe plague bacillus in broth, 25

Tetanus and gangrene organisms. Recentdevelopments concerning the anaerobicbacteria and their activities, withparticular reference to. Abstract, 577

Tetrault, P. A. Abstract, 576Texas Branch, S. A. B., Proceedings, 218Thiamine, Antibacterial action of a pyri-

dine analogue of, 483Thiobacillus thiooxidans, A cytological and

microchemical study of, 451Thompson, Roy C., 99Throats, human, Increased incidence of

virus inclusion bodies in. Abstract, 110Thurman, D. C., and Moore, Donald V.

Abstract, 220Tooth pulp cavities, Poliomyelitis induced

by inoculation of, 311Townsend, C. T., and Zuch, T. L., 269Treponema pallidum, A comparison of the

spirocheticidal action and reagin titerof sera of rabbits infected- for varyingperiods of time with. Abstract, 485

, T. macrodentium and T. microdentium,Bacterial morphology as shown by theelectron microscope. V, 15

Trimethylamine test, A primary divisionof the genus Shigella based on the, 106

Trypanosoma cruzi, infection in Triatomaspp. collected in Texas, A survey of.Abstract. (No abstract), 219

Tumors, Absence of d-amino acids in theproteins of virus-induced, in rabbits.Abstract. (No abstract), 573

Turbidimetric studies on penicillin inhibi-tion. Microbiological aspects of peni-cillin. II, 377

587

INDEX

Tyrosine decarboxylation, Growth require-ments of Streptococcus fecalis for. Ab-stract, 573

Tyrothricin, Formation of, in submergedcultures of Bacillu brevis, 83

Vaccine and antiserum, Epidemic influenza.Abstract, 577

Vaughn, R. Abstract. (No abstract), 223Vaughn, Reese H., 394-, and Gililland, J. Richard, 315Virus, A comparison of the action of sulfa-

drugs on the growth of a bacterial, andof its host. Abstract, 574

- diseases, The laboratory in the diagnosisof. Abstract. (No abstract), 220

-, inclusion bodies, Increased incidence of,in human throats. Abstract, 110

-of lymphocytic choriomeningitis, Associ-ation of the, with erythrocytes of in-fected animals, 482

-, Persistence of the, of St. Louis encepha.litis in the central nervous system ofmice for over five months, 113

Viruses, bacterial, Dissociation of thegrowth of, and of their host by meansof temperatures above optimum. Ab-stract, 575

, Electron microscope studies of bacterial,57

Vitamin B complex, The growth require-ments of Leuconostoc mesenteroides andpreliminary studies on its use as anassay agent for several members ofthe, 441

- C, Decomposition of, by bacteria, 351

Waksman, Selman A., 299; Abstract. (Noabstract), 222

- and Henrici, A. T., 337- and Starkey, Robert L., 489Warren, George, Johnson, Frank H., and

Zworykin, Nina, 167Washington Branch, S.A.B., Proceedings,

217Weiss, Lucile J., Abstract. 576Wells, W. F., 549Westgate, P. J., and Wilson, J. K. Ab-

stract, 222Wheeler, Kenneth M., and Borman, Earle

K., 481

Wheeler, S. M., and Foley, G. E., 391White, Philip R., and Braun, Armin C.

Abstract, 109Wickerham, Lynferd J., 501Wilker, Bernard L., and Foster, Jackson

W., 377Wilson, J. K. Abstract. (No abstract),

222- and Westgate, P. J. Abstract, 222Wintersteiner, O., Menzel, Arthur E. O.,

and Rake, Geoffrey. Abstract, 109Wise, Bowman, and Kerby, Grace P., 333Wood, A. J., Baird, Elizabeth A., and Keep-

ing, Frances E., 106Woodruff, H. Boyd, and Foster, Jackson

W., 187, Foster, J. W., and McDaniel, L. E., 421

Woodward, C. R., Jr., and Stokes, J. L., 83, Stokes, J. L. and Foster, J. W. Ab-

stract, 109Worley, Gordon, Jr., and Worley, Jane

Read. Abstract, 219Worley, Jane Read, and Worley, Gordon, Jr.

Abstract, 219Wright, Marjory H., and Koser, Stewart A.,

239Wyss, Orville, 483

Yeast extract, An unidentified growthfactor found in. Nutritional studies onStreptococcus lactis. I., 369

Yeast-like fungi, The occurrence of naturalantibodies against certain. Abstract,486

Yeasts, A simple technique for the detectionof melibiose-fermenting, 501

, commercial, Selecting, inbreeding, re-combining and hybridizing, 405

-, Nicotinic requirements of certain, 435Young, Gerald, Anigstein, Ludwik, and

Bader, Madero N. Abstract, 218Young, Raymond M., and Rettger, Leo F.,

351Yuncker, T. G. Abstract, 576

ZoBell, Claude E., 39Zuch, T. L., and Townsend, C. T., 269Zworykin, Nina, Johnson, Frank H., and

Warren, George, 167

588

JOURNAL OF BACTERIOLOGY

Manuscripts for the JOURNAL of BACTERIOLOGY should be sent to Prof. C.-E. A. Winslow, YaleMedical School, New Haven, Conn. Manuscripts for BACTERIOLOGICAL kEVEEWS should be sent toProf. Barnett Cohen, Johns Hopkins Medical School, 710 N. Washington Street, Baltimore, Md.

Tweny-five reprints, without covers, of articles will be furnished gratis to contributors when orderedin advance. A table showing cost of additional reprints, with an order slip, is sent with proof.

Correspondence concerning business matters should be addressed to The Williams & Wilkins Company,Mount Royal and Guilford Avenues, Baltimore-2, U. S. A.

THE JOURNAL OF BACTERIOLOGY is issued monthly. (From 1916-1926 Volumes I-XII, inclusivepublished bi-monthly.) Two volumes a year January-June and July-December, are published.

The Subscription price for both volumes will be $10.00. Price of single volume (January-June orJuly-December) $5.00 per v,olume. For countries outside the Postal Union, add 50 cents a volume.The subscriptions of members of the Society of American Bacteriologists are included in the mem-bership dues and are handled by the Association's Treasurer, Dr. W. B. Sarles, University of Wis-consin, Madison, Wisconsin. All other subscriptions should be sent to the publishers.

Claims for copies lost in the mails must be received within 30 days (domestic). For the durationof the war, delivery overseas cannot be guaranteed and must be at subscriber's risk. Changes ofaddress must be received within two weeks of the date of issue.

New subscriptions and renewals are entered to begin with the first issue of the current volume. Shouldany issue of the current volume be out of print at the time the subscription order is received, the pro-rata value of such numbers will be credited to the next volume, and the renewal invoice therefor ad-justed accordingly.

Subscriptions should be renewed promptly-To avoid a break in your series, subscriptions should berenewed promptly. The publishers cannot guarantee to supply back issues on related renewals.

Subscriptions, new or renewal, should be sent to the Publishers or to Agents listed below.

AGENTSFor Australia: Angus & Robertson, Limited, 89-95 Castlereagh Street, Sydney.For the British Empire, except Australia and Canada: Bailliare, Tindall & Cox,

8 HenriettaoSt., Covent Garden, W. C. 2, London, England.For Canada: Wm. Dawson & Sons, Ltd., 70 King Street East, Toronto.

THE WILLIAMS & WILKINS COMPANYPublishers of Scientific Books and Periodicals

BALTIMORE-2, U. S. A.

PUBLISHERS OF: Medicine, Journal of Urology, Journal of Pharmacology and Experimental Therapeutics, American Journalof Tropical Medicine, Journal of Immunology, Journal of Industrial Hygiene and Toxicology, Quarterly Revieto of Biology,Journal of Bacteriology, Chemical Reviews, Soil Science, Social Forces, Journal of Comparative Psychology, OccupationalTherapy and Rehabilitation, The American Journal Qf Clinical Pathology, Journal of Physical Chemistry, Philosophyl ofScience, Journal of Organic Chemistry, Medical Classics, Bacteriological Reviews, Human Fertility, Medical Care, Psycho-somatic Medicine, Gastroenterology.

1=1

I

FORTY-FIFTH MEETINGOF

THE SOCIETY OFAMERICAN BACTERIOLOGISTS

New York City, May 3rd, 4th, and 5th, 1944(Wed., Thurs., Fri.)

The scientific program will include papers on the various phases of bacteriologyand related immunological phenomena. In so far as restrictions will permit, topicsrelating to wartime problems in bacteriology will be emphasized.Round table and panel discussions are being arranged and one or more general

sessions of wide interest are anticipated. A copy of the preliminary program willbe sent to all members of the Society and those who register at the meeting willreceive a copy of the abstracts of all papers. These abstracts will be published inthe May issue of the Journal of Bacteriology.

Abstracts of papers to be presented at the meeting must not exceed 250 wordsin length and must be received by the Chairman of the Program Committee on orbefore February 14th, 1944. Abstracts received after this date will not be accepted.The various committees in charge of the meeting are as follows:

Program CommitteeChairman: L. S. MCCLUNG, Bacteriological Laboratories, 302 Chemistry Building, IndianaUniversity, Bloomington, IndianaVice-Chairman: COLIN M. MAcLEo0

Publicity CommitteeChairman: COLIN M. MAcLEO0, New York University, College of Medicine, New York,New York

Local Committee in charge of arrangementsChairman: MARY B. HORTON, Sheffield Farms, 524 West 57th Street, New York, New YorkVice-Chairman: W. W. BROWNE, City College of New York, 139th Street and ConventAvenue, New York, New York

Division of Medical Bacteriology, Immunology and Comparative ProblemsrChairman: L. A. JULIANELLE, Public Health Institute of The City of New York, Foot ofEast 15th Street, New York, New YorkVice-Chairman: JOHN G. KIDD, Rockefeller Institute, 66th Street and York Avenue, NewYork, New York

Division of Agricutural and Industrial BacteriologyChairman: ELIZABETH McCoy, Department of Agricultural Bacteriology, University ofWisconsin, Madison 6, WisconsinVice-Chairman: R. P. TITTSLER, U. S. Department of Agriculture, Bureau of Dairy Industry,Washington 25, D. C.

The meetings will be held at the Hotel Penn&ylvania in New York City.Reservations for rooms should be made with the hotel as soon as possible.

Society members desiring low priced accommodations should return the officialhotel reservation card 'at the earliest possible date. Special rates for graduatestudents may be had upon application to the hotel or to the Chairman of theLocal Committee.

Exhibits will be under the general direction of C. Virginia Fisher, Warner Insti-tute for Therapeutic Research, 113 West 18th Street, New York, New York, withwhom all exhibitors should communicate before February 14th.