kees de jong senior teacher anatomy ... - plastination.org tol… · basic principles of sheet...
TRANSCRIPT
![Page 1: Kees de Jong Senior teacher Anatomy ... - plastination.org Tol… · Basic principles of sheet plastination: 1 cutting/sawing 2 fixation 3 dehydration 4 impregnation 5 curing. Anatomy](https://reader035.vdocuments.net/reader035/viewer/2022081615/5fe47cbba95a077b755cc000/html5/thumbnails/1.jpg)
Anatomy & Embryology
Kees de Jong
Senior teacher Anatomy & Embryology
Academic Medical Center
dept. of Anatomy & Embryology,
Amsterdam, the Netherlands
plastinator since 1991
![Page 2: Kees de Jong Senior teacher Anatomy ... - plastination.org Tol… · Basic principles of sheet plastination: 1 cutting/sawing 2 fixation 3 dehydration 4 impregnation 5 curing. Anatomy](https://reader035.vdocuments.net/reader035/viewer/2022081615/5fe47cbba95a077b755cc000/html5/thumbnails/2.jpg)
Anatomy & Embryology
![Page 3: Kees de Jong Senior teacher Anatomy ... - plastination.org Tol… · Basic principles of sheet plastination: 1 cutting/sawing 2 fixation 3 dehydration 4 impregnation 5 curing. Anatomy](https://reader035.vdocuments.net/reader035/viewer/2022081615/5fe47cbba95a077b755cc000/html5/thumbnails/3.jpg)
Anatomy & Embryology
Basic principles of sheet plastination:
1 cutting/sawing
2 fixation
3 dehydration
4 impregnation
5 curing
![Page 4: Kees de Jong Senior teacher Anatomy ... - plastination.org Tol… · Basic principles of sheet plastination: 1 cutting/sawing 2 fixation 3 dehydration 4 impregnation 5 curing. Anatomy](https://reader035.vdocuments.net/reader035/viewer/2022081615/5fe47cbba95a077b755cc000/html5/thumbnails/4.jpg)
Anatomy & Embryology
deli slicer for soft tissues,
specimen preferabel embedded in gelatine
slicing
![Page 5: Kees de Jong Senior teacher Anatomy ... - plastination.org Tol… · Basic principles of sheet plastination: 1 cutting/sawing 2 fixation 3 dehydration 4 impregnation 5 curing. Anatomy](https://reader035.vdocuments.net/reader035/viewer/2022081615/5fe47cbba95a077b755cc000/html5/thumbnails/5.jpg)
Anatomy & Embryology
specimen containing
bone:
deepfrozen, sliced with
a band-saw
slicing
![Page 6: Kees de Jong Senior teacher Anatomy ... - plastination.org Tol… · Basic principles of sheet plastination: 1 cutting/sawing 2 fixation 3 dehydration 4 impregnation 5 curing. Anatomy](https://reader035.vdocuments.net/reader035/viewer/2022081615/5fe47cbba95a077b755cc000/html5/thumbnails/6.jpg)
Anatomy & Embryology
when embalmed specimen are used
before starting dehydration:
remove unwanted chemicals from the specimen:
glycols, glycerin: submerge in ethanol 50%
for 1 week, repeat once
slicing
![Page 7: Kees de Jong Senior teacher Anatomy ... - plastination.org Tol… · Basic principles of sheet plastination: 1 cutting/sawing 2 fixation 3 dehydration 4 impregnation 5 curing. Anatomy](https://reader035.vdocuments.net/reader035/viewer/2022081615/5fe47cbba95a077b755cc000/html5/thumbnails/7.jpg)
Anatomy & Embryology
ethanol, (long) alcohols, formalin:
rinse in running tap water for a few days
(depending on the size of the specimen)
when embalmed specimen are used
before starting dehydration:
remove unwanted chemicals from the specimen:
slicing
![Page 8: Kees de Jong Senior teacher Anatomy ... - plastination.org Tol… · Basic principles of sheet plastination: 1 cutting/sawing 2 fixation 3 dehydration 4 impregnation 5 curing. Anatomy](https://reader035.vdocuments.net/reader035/viewer/2022081615/5fe47cbba95a077b755cc000/html5/thumbnails/8.jpg)
Anatomy & Embryology
glycols & glycerin act as anti-freeze, so freezing
prior to sawing has to be done at a low temperature
slicing
![Page 9: Kees de Jong Senior teacher Anatomy ... - plastination.org Tol… · Basic principles of sheet plastination: 1 cutting/sawing 2 fixation 3 dehydration 4 impregnation 5 curing. Anatomy](https://reader035.vdocuments.net/reader035/viewer/2022081615/5fe47cbba95a077b755cc000/html5/thumbnails/9.jpg)
Anatomy & Embryology
specimen are placed in a polystyrene box
slicing
![Page 10: Kees de Jong Senior teacher Anatomy ... - plastination.org Tol… · Basic principles of sheet plastination: 1 cutting/sawing 2 fixation 3 dehydration 4 impregnation 5 curing. Anatomy](https://reader035.vdocuments.net/reader035/viewer/2022081615/5fe47cbba95a077b755cc000/html5/thumbnails/10.jpg)
Anatomy & Embryology
kept in place by wooden sticks and submerged
in water, then deep-frozen
slicing
![Page 11: Kees de Jong Senior teacher Anatomy ... - plastination.org Tol… · Basic principles of sheet plastination: 1 cutting/sawing 2 fixation 3 dehydration 4 impregnation 5 curing. Anatomy](https://reader035.vdocuments.net/reader035/viewer/2022081615/5fe47cbba95a077b755cc000/html5/thumbnails/11.jpg)
Anatomy & Embryology
and sawed at the desired thickness, preferred 3 mm.
slicing
![Page 12: Kees de Jong Senior teacher Anatomy ... - plastination.org Tol… · Basic principles of sheet plastination: 1 cutting/sawing 2 fixation 3 dehydration 4 impregnation 5 curing. Anatomy](https://reader035.vdocuments.net/reader035/viewer/2022081615/5fe47cbba95a077b755cc000/html5/thumbnails/12.jpg)
Anatomy & Embryology
how to set the desired thickness??
slicing
![Page 13: Kees de Jong Senior teacher Anatomy ... - plastination.org Tol… · Basic principles of sheet plastination: 1 cutting/sawing 2 fixation 3 dehydration 4 impregnation 5 curing. Anatomy](https://reader035.vdocuments.net/reader035/viewer/2022081615/5fe47cbba95a077b755cc000/html5/thumbnails/13.jpg)
Anatomy & Embryology
slicing
![Page 14: Kees de Jong Senior teacher Anatomy ... - plastination.org Tol… · Basic principles of sheet plastination: 1 cutting/sawing 2 fixation 3 dehydration 4 impregnation 5 curing. Anatomy](https://reader035.vdocuments.net/reader035/viewer/2022081615/5fe47cbba95a077b755cc000/html5/thumbnails/14.jpg)
Anatomy & Embryology
slicing
![Page 15: Kees de Jong Senior teacher Anatomy ... - plastination.org Tol… · Basic principles of sheet plastination: 1 cutting/sawing 2 fixation 3 dehydration 4 impregnation 5 curing. Anatomy](https://reader035.vdocuments.net/reader035/viewer/2022081615/5fe47cbba95a077b755cc000/html5/thumbnails/15.jpg)
Anatomy & Embryology
slicing
![Page 16: Kees de Jong Senior teacher Anatomy ... - plastination.org Tol… · Basic principles of sheet plastination: 1 cutting/sawing 2 fixation 3 dehydration 4 impregnation 5 curing. Anatomy](https://reader035.vdocuments.net/reader035/viewer/2022081615/5fe47cbba95a077b755cc000/html5/thumbnails/16.jpg)
Anatomy & Embryology
slicing
![Page 17: Kees de Jong Senior teacher Anatomy ... - plastination.org Tol… · Basic principles of sheet plastination: 1 cutting/sawing 2 fixation 3 dehydration 4 impregnation 5 curing. Anatomy](https://reader035.vdocuments.net/reader035/viewer/2022081615/5fe47cbba95a077b755cc000/html5/thumbnails/17.jpg)
Anatomy & Embryology
cleaning the section from sawdust
slicing
![Page 18: Kees de Jong Senior teacher Anatomy ... - plastination.org Tol… · Basic principles of sheet plastination: 1 cutting/sawing 2 fixation 3 dehydration 4 impregnation 5 curing. Anatomy](https://reader035.vdocuments.net/reader035/viewer/2022081615/5fe47cbba95a077b755cc000/html5/thumbnails/18.jpg)
Anatomy & Embryology
slicing
![Page 19: Kees de Jong Senior teacher Anatomy ... - plastination.org Tol… · Basic principles of sheet plastination: 1 cutting/sawing 2 fixation 3 dehydration 4 impregnation 5 curing. Anatomy](https://reader035.vdocuments.net/reader035/viewer/2022081615/5fe47cbba95a077b755cc000/html5/thumbnails/19.jpg)
Anatomy & Embryology
slicing
![Page 20: Kees de Jong Senior teacher Anatomy ... - plastination.org Tol… · Basic principles of sheet plastination: 1 cutting/sawing 2 fixation 3 dehydration 4 impregnation 5 curing. Anatomy](https://reader035.vdocuments.net/reader035/viewer/2022081615/5fe47cbba95a077b755cc000/html5/thumbnails/20.jpg)
Anatomy & Embryology
slicing
![Page 21: Kees de Jong Senior teacher Anatomy ... - plastination.org Tol… · Basic principles of sheet plastination: 1 cutting/sawing 2 fixation 3 dehydration 4 impregnation 5 curing. Anatomy](https://reader035.vdocuments.net/reader035/viewer/2022081615/5fe47cbba95a077b755cc000/html5/thumbnails/21.jpg)
Anatomy & Embryology
acetone
acetone
acetone
acetone
acetone
acetone
acetone
acetone
acetone
acetone
acetone
acetone
acetone
acetone
acetone
slicing
![Page 22: Kees de Jong Senior teacher Anatomy ... - plastination.org Tol… · Basic principles of sheet plastination: 1 cutting/sawing 2 fixation 3 dehydration 4 impregnation 5 curing. Anatomy](https://reader035.vdocuments.net/reader035/viewer/2022081615/5fe47cbba95a077b755cc000/html5/thumbnails/22.jpg)
Anatomy & Embryology
slicing
![Page 23: Kees de Jong Senior teacher Anatomy ... - plastination.org Tol… · Basic principles of sheet plastination: 1 cutting/sawing 2 fixation 3 dehydration 4 impregnation 5 curing. Anatomy](https://reader035.vdocuments.net/reader035/viewer/2022081615/5fe47cbba95a077b755cc000/html5/thumbnails/23.jpg)
Anatomy & Embryology
specimen
formaline fixed embalmed
dehydrationremoval of
glycerine, phenol, etc.
dehydration
dehydration
![Page 24: Kees de Jong Senior teacher Anatomy ... - plastination.org Tol… · Basic principles of sheet plastination: 1 cutting/sawing 2 fixation 3 dehydration 4 impregnation 5 curing. Anatomy](https://reader035.vdocuments.net/reader035/viewer/2022081615/5fe47cbba95a077b755cc000/html5/thumbnails/24.jpg)
Anatomy & Embryology
specimen
formaline fixed embalmed
dehydrationremoval of
glycerine, phenol, etc.
dehydration
dehydration
![Page 25: Kees de Jong Senior teacher Anatomy ... - plastination.org Tol… · Basic principles of sheet plastination: 1 cutting/sawing 2 fixation 3 dehydration 4 impregnation 5 curing. Anatomy](https://reader035.vdocuments.net/reader035/viewer/2022081615/5fe47cbba95a077b755cc000/html5/thumbnails/25.jpg)
Anatomy & Embryology
removal of glycerine, phenol, etc.
dehydration
![Page 26: Kees de Jong Senior teacher Anatomy ... - plastination.org Tol… · Basic principles of sheet plastination: 1 cutting/sawing 2 fixation 3 dehydration 4 impregnation 5 curing. Anatomy](https://reader035.vdocuments.net/reader035/viewer/2022081615/5fe47cbba95a077b755cc000/html5/thumbnails/26.jpg)
Anatomy & Embryology
10-30°
dehydration
![Page 27: Kees de Jong Senior teacher Anatomy ... - plastination.org Tol… · Basic principles of sheet plastination: 1 cutting/sawing 2 fixation 3 dehydration 4 impregnation 5 curing. Anatomy](https://reader035.vdocuments.net/reader035/viewer/2022081615/5fe47cbba95a077b755cc000/html5/thumbnails/27.jpg)
Anatomy & Embryology
dehydration
![Page 28: Kees de Jong Senior teacher Anatomy ... - plastination.org Tol… · Basic principles of sheet plastination: 1 cutting/sawing 2 fixation 3 dehydration 4 impregnation 5 curing. Anatomy](https://reader035.vdocuments.net/reader035/viewer/2022081615/5fe47cbba95a077b755cc000/html5/thumbnails/28.jpg)
Anatomy & Embryology
dehydration
![Page 29: Kees de Jong Senior teacher Anatomy ... - plastination.org Tol… · Basic principles of sheet plastination: 1 cutting/sawing 2 fixation 3 dehydration 4 impregnation 5 curing. Anatomy](https://reader035.vdocuments.net/reader035/viewer/2022081615/5fe47cbba95a077b755cc000/html5/thumbnails/29.jpg)
Anatomy & Embryology
refresh the ethanol after 1 week
rinse in running tapwater
immerse in ethanol 50% for another week,
move every day
dehydration
![Page 30: Kees de Jong Senior teacher Anatomy ... - plastination.org Tol… · Basic principles of sheet plastination: 1 cutting/sawing 2 fixation 3 dehydration 4 impregnation 5 curing. Anatomy](https://reader035.vdocuments.net/reader035/viewer/2022081615/5fe47cbba95a077b755cc000/html5/thumbnails/30.jpg)
Anatomy & Embryology
Monitoring of the rate of
dehydration
dehydration
![Page 31: Kees de Jong Senior teacher Anatomy ... - plastination.org Tol… · Basic principles of sheet plastination: 1 cutting/sawing 2 fixation 3 dehydration 4 impregnation 5 curing. Anatomy](https://reader035.vdocuments.net/reader035/viewer/2022081615/5fe47cbba95a077b755cc000/html5/thumbnails/31.jpg)
Anatomy & Embryology
measurement of the rate of dehydration
=
measurement of specific weight of the
dehydration bath
dehydration
![Page 32: Kees de Jong Senior teacher Anatomy ... - plastination.org Tol… · Basic principles of sheet plastination: 1 cutting/sawing 2 fixation 3 dehydration 4 impregnation 5 curing. Anatomy](https://reader035.vdocuments.net/reader035/viewer/2022081615/5fe47cbba95a077b755cc000/html5/thumbnails/32.jpg)
Anatomy & Embryology
acetone specific weight= 0.8
water specific weight= 1.0
ethanol specific weight= 0.8
acetone
water
sw = 0.9
= 50%
mix
dehydration
![Page 33: Kees de Jong Senior teacher Anatomy ... - plastination.org Tol… · Basic principles of sheet plastination: 1 cutting/sawing 2 fixation 3 dehydration 4 impregnation 5 curing. Anatomy](https://reader035.vdocuments.net/reader035/viewer/2022081615/5fe47cbba95a077b755cc000/html5/thumbnails/33.jpg)
Anatomy & Embryology
acetone
ethanolmix
sw = 0.8
≠100%
dehydration
acetone specific weight= 0.8
water specific weight= 1.0
ethanol specific weight= 0.8
![Page 34: Kees de Jong Senior teacher Anatomy ... - plastination.org Tol… · Basic principles of sheet plastination: 1 cutting/sawing 2 fixation 3 dehydration 4 impregnation 5 curing. Anatomy](https://reader035.vdocuments.net/reader035/viewer/2022081615/5fe47cbba95a077b755cc000/html5/thumbnails/34.jpg)
Anatomy & Embryology
so, rinse specimens with ethanol
in running tapwater until ethanol is
replaced with water
then start dehydration
dehydration
![Page 35: Kees de Jong Senior teacher Anatomy ... - plastination.org Tol… · Basic principles of sheet plastination: 1 cutting/sawing 2 fixation 3 dehydration 4 impregnation 5 curing. Anatomy](https://reader035.vdocuments.net/reader035/viewer/2022081615/5fe47cbba95a077b755cc000/html5/thumbnails/35.jpg)
Anatomy & Embryologyacetone : specimen’s water = 10 : 1
acetone 100%
acetone
water
dehydration
![Page 36: Kees de Jong Senior teacher Anatomy ... - plastination.org Tol… · Basic principles of sheet plastination: 1 cutting/sawing 2 fixation 3 dehydration 4 impregnation 5 curing. Anatomy](https://reader035.vdocuments.net/reader035/viewer/2022081615/5fe47cbba95a077b755cc000/html5/thumbnails/36.jpg)
Anatomy & Embryology
acetone 90%
water 10%
acetone 90%
water 10%
repeat the procedure
dehydration
![Page 37: Kees de Jong Senior teacher Anatomy ... - plastination.org Tol… · Basic principles of sheet plastination: 1 cutting/sawing 2 fixation 3 dehydration 4 impregnation 5 curing. Anatomy](https://reader035.vdocuments.net/reader035/viewer/2022081615/5fe47cbba95a077b755cc000/html5/thumbnails/37.jpg)
Anatomy & Embryologyacetone : specimen’s water = 10 : 1
acetone 100%
acetone
water
dehydration
![Page 38: Kees de Jong Senior teacher Anatomy ... - plastination.org Tol… · Basic principles of sheet plastination: 1 cutting/sawing 2 fixation 3 dehydration 4 impregnation 5 curing. Anatomy](https://reader035.vdocuments.net/reader035/viewer/2022081615/5fe47cbba95a077b755cc000/html5/thumbnails/38.jpg)
Anatomy & Embryology
acetone 99%
water 1%
acetone 99%
water 1%
repeat untill acetone is >98,5%
dehydration
![Page 39: Kees de Jong Senior teacher Anatomy ... - plastination.org Tol… · Basic principles of sheet plastination: 1 cutting/sawing 2 fixation 3 dehydration 4 impregnation 5 curing. Anatomy](https://reader035.vdocuments.net/reader035/viewer/2022081615/5fe47cbba95a077b755cc000/html5/thumbnails/39.jpg)
Anatomy & Embryology
best performed with cold acetone (-25º C)
1 pre-cool specimen to 5º C
2 keep specimen completely submerged
to prevent air drying
freeze substitution
dehydration
![Page 40: Kees de Jong Senior teacher Anatomy ... - plastination.org Tol… · Basic principles of sheet plastination: 1 cutting/sawing 2 fixation 3 dehydration 4 impregnation 5 curing. Anatomy](https://reader035.vdocuments.net/reader035/viewer/2022081615/5fe47cbba95a077b755cc000/html5/thumbnails/40.jpg)
Anatomy & Embryology
impregnation
Impregnation of polyester:
1 is performed at room temperature
2 vacuum is increased quick (< 1day)
3 vacuum is not deeper than 15 mm Hg.
![Page 41: Kees de Jong Senior teacher Anatomy ... - plastination.org Tol… · Basic principles of sheet plastination: 1 cutting/sawing 2 fixation 3 dehydration 4 impregnation 5 curing. Anatomy](https://reader035.vdocuments.net/reader035/viewer/2022081615/5fe47cbba95a077b755cc000/html5/thumbnails/41.jpg)
Anatomy & Embryology
Lower pressure will extract styrene molecules from
the polyester impregnation bath, resulting in:
improper impregnation and curing;
damaging the vacuumpump
![Page 42: Kees de Jong Senior teacher Anatomy ... - plastination.org Tol… · Basic principles of sheet plastination: 1 cutting/sawing 2 fixation 3 dehydration 4 impregnation 5 curing. Anatomy](https://reader035.vdocuments.net/reader035/viewer/2022081615/5fe47cbba95a077b755cc000/html5/thumbnails/42.jpg)
Anatomy & Embryology
impregnation
![Page 43: Kees de Jong Senior teacher Anatomy ... - plastination.org Tol… · Basic principles of sheet plastination: 1 cutting/sawing 2 fixation 3 dehydration 4 impregnation 5 curing. Anatomy](https://reader035.vdocuments.net/reader035/viewer/2022081615/5fe47cbba95a077b755cc000/html5/thumbnails/43.jpg)
Anatomy & Embryology
impregnation
![Page 44: Kees de Jong Senior teacher Anatomy ... - plastination.org Tol… · Basic principles of sheet plastination: 1 cutting/sawing 2 fixation 3 dehydration 4 impregnation 5 curing. Anatomy](https://reader035.vdocuments.net/reader035/viewer/2022081615/5fe47cbba95a077b755cc000/html5/thumbnails/44.jpg)
Anatomy & Embryology
impregnation
When bubbles stop rising, impregnation is complete.
Depending on the amount of specimen this will take
1 – 3 days.
![Page 45: Kees de Jong Senior teacher Anatomy ... - plastination.org Tol… · Basic principles of sheet plastination: 1 cutting/sawing 2 fixation 3 dehydration 4 impregnation 5 curing. Anatomy](https://reader035.vdocuments.net/reader035/viewer/2022081615/5fe47cbba95a077b755cc000/html5/thumbnails/45.jpg)
Anatomy & Embryology
QUESTIONS???