FPE 2153

TISSUE CULTURE TECHNOLOGY

KELANTAN BIOTECH CORPORATION SDN. BHD

1) NURUL AIN NABILAH BINTI MOHAMAD ZAWAWI (B11A329)2) AZIFAH BINTI MOHD SUHAIMI (B11A038)3) NOR SYAKIRA BINTI MAT (B11A289)4) FARAH NUR AYU BINTI AHMAD (B11A068)5) MOHD ADIB BIN MOHD NAFI (B11A201)6) AHMAD IQBAL BIN MUHAMAD (B11A016)

OBJECTIVES

To being exposed with tissue culture industry To gain knowledgeable information To further understand the steps involved in tissue culture technique To see how the tissue culture being commercialized

INTRODUCTION

Kelantan Biotech Corporation Sdn. Bhd. products are made to industrial, agriculture and health sector.

The main office is located at Labok, Machang

Consist of 3 section that are lab for plant tissue culture, nursery for planting the successful plant culture that transferred from lab, and the last is the factory that produce Effective Microorganism(EM).

A first company that do plant tissue culture in Kelantan.

They also produce the ornamental plants and herbs for example orchids and taro

mice (keladi tikus).

also carried out research (R & D) for improvement.

The prices offered are affordable and KBCSB also ready to take orders from people if they are interested to make reservations and buy

tissue culture plantlets.

POTENTIAL OF TISSUE CULTURE IN AGRICULTURE

can be used to produce thousand clones of a plant at shorter time( increasing the

production potential) can support the high food stock demand

able to culture new plants though the climates are not appropriate to produce

a plant. Moreover, if seed is not available, it is possible to produce a

plant with this method.

it can create large number of clones from single explant that are healthy, free from

diseases, uniform in their shape and output, plants with better flowers, odors, fruits or any other properties of the plants that are beneficial to the human beings.

can support agricultural crop improvement

the tissue culture technique was help to generate banana saplings is one of the

solutions to help farmers to grow bananas on commercial scale.

Bacterial and fungi contaminationNew skilled worker – takes time to train them

Energy supply – electricity somaclonal variation (off-type) - cause economical lost Technical problem to develop much bigger scale production

CHALENGES

COMPARISON

Kelantan biotech FELDA HEXAGON

Tissue culture, GMP, Enzyme

Tissue culture Tissue culture

Main product in tissue culture is banana and ornamental.

Main product in tissue culture is palm oil and banana.

Main product in tissue culture is banana and ornamental plant.

Provide plant to Kelantan area and around Malaysia

Provide plant to FELDA around Malaysia also the outsiders

Provide plant especially orchid cut flower to overseas.

Scale of production per month about 20 000.

1 million/ year -

Provide consultancy and advisory services

Provide consultancy and advisory services

Provide consultancy and advisory services

STRENGTH

Variety of tissue cultured plant

producedThe only tissue

culture company in

KelantanHave good equipmentThe parent

plant taken to be cultured is certified and have good

qualityHave high capacity storage

room

Have price promotion

WEAKNESS

Cannot yet achieved daily production rate

Not enough experienced

tissue culturist

High cost of maintenances

Small culture room

Limited time of operation

OPPORTUNITY

Can be a large supplier of

tissue cultured plant to farmers

High demand of tissue cultured

plant by farmers

Can become the supplier ornamental

tissue cultured plant

THREATS

High rate of contamination

Does not have a backup generator

Hard to find an experienced

worker

SUGGESTION

Give more training to the new comers in plant tissue culture

technique.Increase the production rate by

increasing the number of expertise worker.

Need a spacious place for the worker to do their work

comfortable. Restrict the rule in the laboratory

to avoid contamination

CONCLUSIONMany useful knowledge were gain.Able to make analysis on tissue culture industry.Contamination is the major lost in tissue culture.