Kevin BakerAsad Mansoor

Welcome to 181LSection 14 Room 430

This lab is about you:Thinking, Evaluating, Investigating and Understanding

This lab is NOT about you:Showing up, jumping through hoops and

leaving

We are not synchronized with the lectures, but cover ‘same’ material

Welcome to 181L

• Read the lab manual BEFORE lab• Be ON TIME to class• PARTICIPATE• Complete the assignments (online, etc)• Participate• Think, evaluate, investigate, understand

Expectations

• Phones, etc• Food and drinks• C’mon, really?• Code of Conduct http://deanofstudents.arizona.edu/studentcodeofconduct

Expectations

• Info found in syllabus• Assignments• Online assessments/tutorials 10%• In-class quizzes 15%• Lab-based assignments 50%• Projects and Reports 25%

Grading

• Absences must be excused through Asya Roberts in BSE 109• Arriving late/leaving early is absent• Make-up labs are labs. Only current week• Miss 2 labs and you should drop, or we’ll do it

Attendance

• Just do YOUR work• Plagiarism agreement p xii• Link http://deanofstudents.arizona.edu/codeofacademicintegrity/

Plagiarism

• Class webpage:http://blc.arizona.edu/courses/181lab

• Click ‘Create 181L Account’• Our section number is ‘14’• Let me know about you• ‘Bio181L_Go’ is best way to do online homework• Links available under ‘course software’

181L

181L

181L

Molecules and the Tao of the Molecular World

Molecules and the Tao of the Molecular World

Molecules and the Tao of the Molecular World

Molecules and the Tao of the Molecular World

How molecules feel, and the world they live in

Molecules and the Tao of the Molecular World

• Observe the forces acting on and between macromolecules

Molecules and the Tao of the Molecular World

• Observe the forces acting on and between macromolecules • Make deductions about molecular world from observations

Molecules and the Tao of the Molecular World

• Observe the forces acting on and between macromolecules • Make deductions about molecular world from observations• Become familiar with computer representations of molecules

Molecules and the Tao of the Molecular World

• Observe the forces acting on and between macromolecules • Make deductions about molecular world from observations• Become familiar with computer representations of molecules• Begin to think of biological molecules as real, with distinctive surfaces.

Atoms: They’re how life works

DNA: C, H, N, O, P, [Mg++]RNA: C, H, N, O, P, [Mg++]Protein: C, H, N, O, (S), (P), [traces]Membranes: C, H, N, O, P, (S)

It’s pretty simple, really…

What cannot be done with assemblages of these atoms* cannot be done by living

organisms, nor their cells, nor their spit, etc.

Who Am I?• At birth, # protons = # electrons• Atoms seek completion• Mostly it means 8 in the outer ‘shell’• Hydrogen and Helium are two

ColorsYou’ll need

to know thesefor NEXT week

ColorsRED also means ‘negative’

Blue also means ‘positive’

Dependent on:# protons (+ve charge)Distance e- from nucleus

Slide courtesy of Cara Gibson

A Little JargonBonds/Interactions

Covalent bond: shared pair of electrons* Non-polar bond: equal sharing; “greasy” Polar bond: doesn’t share well with others Hydrogen bond: Hydrogen interaction-- partial(+) H atom attracted to partial(-) oxygen or nitrogen Ionic bond: attraction between opposites

Dependent on:# protons (+ve charge)Distance e- from nucleus

Slide courtesy of Cara Gibson

Looking at Water

Looking at WaterH2O

Looking at WaterH2O

HO

H

Looking at WaterH2O

HO

H

Looking at WaterH2O

HO

H

+

+

-

Looking at Water

To your StructViewers!Desktop => Bio181L_Go

Hydrophobicity (non-polar covalent bonds)

Water is disordered - when lipids present, water molecules arrange in more ordered way.

Clumping together of fat molecules reduces amount of water that has to be ordered.www.steve.gb.com/science/membranes.html

Slide Courtesy of Cara Gibson

How to draw molecules

Carbon Hydrogen Oxygen NitrogenFigure is from Lab Manual p. 0-5

Things to do today…Demonstrate that you get it :

Surface tensionHydrophobicity & your worldHeat helps (molecular movement)Size Matters

Things to do today…• Assessor• primary online tool for labs• You’ll use it today in lab• Many homework assignments

Things to do today…• Assessor• primary online tool for labs• You’ll use it today in lab• Many homework assignmentsThink, Evaluate, Investigate and

Understand

Groups• Tao_MCB181L• Enter assigned group name• Periodically change ‘jobs’• THINK!!!!!!!

AtroxWillardiViridisCerberusCerastesTigrisMolossus

Molecules and the Tao of the Molecular World

• Observe the forces acting on and between macromolecules • Make deductions about molecular world from observations• Become familiar with computer representations of molecules• Begin to think of biological molecules as real, with distinctive surfaces.

Alcohol vs. Water• “If this is true…”• Densities:

• Which one is closest to water?• Oil/Water and/or Salt Water/water

MeOH = 791EtOH = 789PrOH = 803BuOH = 810

Ethanol vs. Water

•Which is bigger/heavier?•Which evaporates/distills first?•Why...?

Clean Up• Oil waste in the hood• Ethanol waste in sink• Anything too messy, throw away• Leave the lab as you found it

Non-newtonian fluids for fun

Running on waterCornstarch on a subwoofer

HomeworkDUE BEFORE 10 PM MONDAY!

1. ‘181 Intro 11’2. ‘Molecular World’ tutorial3. Crossword (2) OR Vocabuwary

Molecules and the Tao of the Molecular World

HomeworkDUE BEFORE 10 PM MONDAY!

1. ‘181 Intro 11’2. ‘Molecular World’ tutorial3. Crossword (2) OR Vocabuwary

Quiz next week’s WILL include information from this week’s lab/assignments

and next week’s lab

HomeworkStart the homework early

e-mail me with questions