Koch’ Postulates

• 병원균과 질병의 지속적 연관성• 분리 , 순수배양• 건강한 개체에 접종시 동일한 증상• 재분리 후 동일한 병원균 확인

Plant vs. Animal

• No antibody, T cell response• No circulating cell• Systemic signal through plant• Disposable and replaceable parts in

plant

Gene for Gene theory

• Avr : avr (avirulence) gene• R : r (resistance) gene

• R * Avr = Resistance• R * avr = Susceptible• r * Avr = Susceptible• R * avr = Susceptible

Signal Molecules

• ROD, reactive oxygen species• NO, nitric oxide• SA, salicylic acid• JA, jasmoic acid• C2H2, ethylene

신호전달 후• HR, hypersensive response• Phytoalexins• PR, pathogenesis related proteins• Cell wall reinforcing, H2O2

• Ca++

• Active oxygens• SA

R and Avr interactions

• PAMP(pathogen associated molecular pattern) through TTSS(type III secretion system), Fls2(flagellin receptor)

• AvrRpm1 increases phosphorylation of Rin4 and decrease PAMP signaling

• AvrRpt2, protease cleaves Rin4

• Rpm1 interact with Rin4 and detect change in Rin4

내병성 육종Exogeneous genes

- pathogen origin (M, Coat proteins, replicase) - plant origin (RIP)

Endogenous genes - SAR - HR - PR - Signal transduction

내병성 분자육종• Engineering of R for broad spectrum• Expression of R in an inducible manner - broad specificity of pathogens - multifactorial defense mechanism - only w/I infected tissue

• 내병성 육종의 한계 : 병원균의 진화