Koh Tze LoongPERSONAL PARTICULARS

Full Name : Koh Tze LoongPassport/ NRIC No. : S8124394HGender : MaleNationality : SingaporeanRace : ChineseMarital Status : SingleNS Status : CompletedReligion : BuddhistLanguage Proficiency : English, Chinese

CONTACT DETAILSEmail : kohtzeloong @gmail.com Main Contact : 96368336 (HP), 65423838 (home) Address : 38 Sunbird Circle Apollo Garden

Singapore 487277

EDUCATION and EMPLOYMENT - SUMMARYHighest Degree : Honours (1st Class)Course of Study : Bachelor of BiotechnologyGraduation : July 2006Years Worked : 7 YearsWork Experience : Honors Project (1 Year) with Associate Professor John Pemberton

Worked for A-star in Bioinformatics Institute (BII) (2 Years) with collaborations with IMCB under Dr Jeffery Hill and Dr Walter Blackstock: Did mostly protein modification work and molecular biology work

Worked in NUH laboratory (CSI) under Prof. Chng Wee Joo. Did various drug studies as well as genetic and molecular work

Employment Status : Research Assistant Hematology-Oncology DepartmentWorking for Assoc Prof Chng Wee Joo

38 Sunbird CircleSingapore 48727796368336kohtzeloong@gmail.com

EDUCATION - DETAILSMasters of Science (National University Singapore) June 2011 - May 2014

University of Queensland, AustraliaHonours (1st Class), Bachelor of Biotechnology Feb 2004 – Jul 2006

Dean’s List, Semester 3, 2005High Distinction for Molecular MicrobiologyDistinctions for: Genomics & Bioinformatics,

Temasek PolytechnicDiploma in General Biotechnology Jul 1998 – Jul 2001

Manjusri Secondary SchoolGCE ‘O’ Level Feb 1994 – Dec 1997

EMPLOYMENT - DETAILSHonors Project with Associate Professor John Maxwell Pemberton July 2005 – June 2006

Fulfillment as part of the Honors Program. Did genetic work on bacteria: Pseudomonas stutzeri, Rhodobacter sphaeroides and Escherichia Coli.

A-Star Bioinformatics Institue (BII) October 2006 – August 2007 Worked for 1.5 Years under Dr Jeffery Hill and Dr Francis Lim Buan Hong in A-STAR Bioinformatics Institute. Did collaborations work there with IMCB. Worked on the cloning and expression of artificial proteins.

ETC (Experimental Therapeutics Center) August 2007 – October 2008 Transferred to ETC with Dr Jeffery Hill, there I helped to set up new laboratory. Did cloning and expression of AIF-SH2 (Apoptosis Inducing Factor-Short version) and AIFM3 (mitochondrion associated) into expression vector Pet21b and expressed in E. coli cell RGB-PlysS (Rosetta-gami B)NUH November 2008 – August 2014 Worked under Assoc. Professor Chng Wee Joo. My duties include

- Immunohistochemistry for multiple collaborators, - Microarray experiments for our own lab publications and - Various forms of drug studies involving anti-cancer drugs like DZNep, Velcade and other test drugs from pharmaceutical companies.

GSK August 2014 - CurrentCurrently working in GSK Vaccines as a Quality Assurance Executive in the Sterility Assurance group.Involved in:

- Tracking Environmental Monitoring Trends for presentation in quarterly reviews. - Handling various deviations in production with relations to environmental out of specifications

or aseptic related deviations.- Oversight of Media Simulations in the isolator for operator and equipment re-qualification- GAP analysis of various sterility related guidelines such as 6003-1 to 6003-4 series. (sterility,

environmental monitoring and aseptic simulations)- Training and qualification of aseptic operators in the site- Writing of rationale papers for Aseptic Media Simulations- Various projects on site with relation to single use systems (Lynx Connectors, Single use bags)

Supervisory experience:Currently designated as backup for team QA Lead. When Lead is absent, duties involve:

- Conducting Tier 1 meetings for SA technicians as well as representing the team in discussions with other teams from production and quality control.

- Organizing team schedule and leading technicians in walk-downs of production areas for sampling as well as during QA oversight.

- Tracking of Environmental monitoring frequency conducted by QA technicians as well as ensuring all sampling is conducted on track.

- Handling any deviations pertaining to usage of expired agar, invalid sampling etc.

Oversight of Media simulations involves making critical decisions with regards to invalidating samples or during spillages or leakage etc.

Currently the SA representative for 1 of 2 buildings in the site, able to make independent decisions with regards to any matters involving Environmental or Sterility matters such as but not limited to:

- Shutdown cleaning frequency. - Actions in the event of HVAC stoppage or pressure cascade.- Out of action levels for biobuden found in CIP samples for various equipments such as SEC

columns, fermenters, fundabacs.

OTHER RELEVANT SKILLSBioinformatics : DNA-Star, Blast Program, TIGR, NCBI, Cluster W,

Primers Designing, Gene Spring Analysis platform. Laboratory Techniques : RT-PCR, Real-Time PCR, Protein Expression and purification

(AIF-SH2, MMP-QCAT) PCR, DNA Sequencing, Plasmid Extractions, Bacterial Cloning, Cell staining with antibodies, Human cell tissue Culture (Myeloma cell lines, Leukemia cell lines, Breast Cancer, Colon Cancer), Western Blot,

Microarray Platforms : Agilent miRNA chips, Illumina Whole Genome DASL chips, Affimetrix 3’IVT labeling chips

ImmunoHistoChemistry:Staining with H&E (Hematoxylin and eosin), Antibody staining with various antibodies (CD31, VWF factor, LIN-28 and PRL-3). Creation of Tissue Microarrays from cell blocks and various tissues.

Flow Cytometry :Worked with BD LSR II machine and performed Apoptosis Assays using FITC Annexin V Apoptosis Kit.

Sterility Assurance :Familiar with Isolator operations as well as various SOPs related to isolator use.Familiar with SAP user system.Familiar with various sterility guidelines as well as media simulation requirements.Familiar with GSK shutdown, line clearance, performance qualifications, validation procedures and change request process.

OTHER SKILLSComputer Applications : Microsoft Office Applications

Driving : Class ‘3’ License

National Service : Completed June 2001 – Dec 2003

Current salary : SGD $4,950

Expected Salary : Negotiable