Slide 1The Nest Group, Inc.

• high quality glass columns• for lab scale to process scale• solvent resistant and/or biocompatible

design• designed for low, medium and high

pressure column chromatography

Kronlab Glass Columns

Slide 2The Nest Group, Inc.

Product Lines

• Eco– lab scale – low / medium pressure

• ECOPLUS

– extended pressure rating up to 80 bar

– lab scale to prep

• Classic– lab scale– medium pressure– conical tube

• BioCart– lab scale to prep– glass cartridges– medium to high pressure

• Pilot– pilot scale to production

scale– custom designed – solvent resistant or for

aqueous buffers

Slide 3The Nest Group, Inc.

Pilot Columns for Pilot to Production Scale

Slide 4The Nest Group, Inc.

Pilot Columns for Pilot to Production Scale

• designed for use in pilot and production scale• designed for use with organic solvents (version SR) or

aqueous buffers (version AB)• outstanding flow distribution for a linear flow

distribution profile• high precision manufacturing of plungers and

distribution plates with CNC milling machines, no injection moulding involved

Slide 5The Nest Group, Inc.

Pilot Columns for Pilot to Production Scale

• full documentation for validation• full service and support for custom designed columns• materials are in compliance with FDA regulations CFR

21, certified if needed• precise and reliable height adjustment of the piston

with hand wheels• mounted either on castors or fixed stand or directly into

customer´s plant

Slide 6The Nest Group, Inc.

Pilot Columns - Wetted Materials

• column tube: calibrated borosilicate glass or stainless steel (steel quality to customer‘s specification); optional acrylic

• piston: PE, PTFE, PVDF or stainless steel• seals: EPDM or Kalrez®• frits: PE, stainless steel, others on request

Slide 7The Nest Group, Inc.

Pilot Columns - Sizes

• columns from 60 mm to 600 mm ID• length on demand

Slide 8The Nest Group, Inc.

Pilot Column -Design of Flow Distributor

flow distribution profile proofed by Bayer AG and Kronlab GmbH

Slide 9The Nest Group, Inc.

Pilot Column -flexible inner diameters

Flow distribution profile proofed by Bayer AG and Kronlab GmbH

PI 60

PI 100PI 100 Cooling Jacket

PI 140

PI 450

Slide 10The Nest Group, Inc.

Pilot Column -flexible column material

stainless steel

glass or acrylic

Slide 11The Nest Group, Inc.

Pilot Column -flexible pressure ranges

1 bar

8,7 bar

10 bar

50 bar

Slide 12The Nest Group, Inc.

Pilot Column -integration of user demands

e.g. rotable frames, integrated level indicator, brackets, selected materials, connectors ……….

Slide 13The Nest Group, Inc.

Pilot Columns -

Custom Designspecial solutions for individual needs regarding:

• materials

• design

• size

• system integration

Slide 14The Nest Group, Inc.

Pilot Columns -

Custom Design

• individual feasibility studies and technical drawings

• certification

• competent guidance throughout the whole project

Slide 15The Nest Group, Inc.

Pilot AB - rotatable

column id: 200 mm

max. bed height: 1600 mm

pressure rating: 7 bar

specially designed rotatable frame construction

Slide 16The Nest Group, Inc.

Pilot SR – solvent resistant

technical specifications:

column id: 200 and 300 mmmax. bed height: 400 mmpressure rating: 1.5 bar

explosion-proof environment with antistatic protection and wheels

Slide 17The Nest Group, Inc.

Pilot SR – stainless steel

technical specifications:

column id: 100 mmmax. bed height: 1000 mmmin bed height: 500 mmpressure rating: 40 barsealing: Kalrez®

Slide 18The Nest Group, Inc.

Pilot SR – stainless steel

technical specifications:

column id: 150 mmmax. bed height: 900 mmmin bed height: 700 mmpressure rating: 50 barsealing: Kalrez®

Slide 19The Nest Group, Inc.

Pilot AB - Reactor

id: 300 mmbed height: 0- 500 mmpressure rating: 1 barfrit: 30 µm

(SS 1.4404)

special features:• slurry inlet• integrated magnetic stirrer on cambered bottom frit• piston with scraper• special designed column stand

Slide 20The Nest Group, Inc.

Pilot AB -rotatable

id: 240 mmbed height: 0 - 270 mmvolume: 0-12 lpressure rating: 3 barcooling jacket: 15 - 65°CSS - container: 15 l for slurry wastefrits: 10 µm PE

5 mm thicknessspecially designed frit fastening screwsto ensure perfect flow distribution

Slide 21The Nest Group, Inc.

Pilot Columns - stainless steel

id: 600 mm

max. bed height: 1000 mm

(1500 mm)

bed volume: 282,6 l

(423,9 l)

pressure rating: 10 bar

frits: 10 µm

Slide 22The Nest Group, Inc.

ExamplePilot Column - stainless steelid: 600 mmmax. bed height: 1000 mm

(1500 mm)bed volume: 282,6 l

(423,9 l)pressure rating: 10 barfrits: 10 µm

designed for repeated packing and unpacking without opening the column

Slide 23The Nest Group, Inc.

Solvent Inlet

Solvent Outlets

Slurry-Outlet through central outlet valve

Example

Slurry-Inlet through separate slurry pump

Slide 24The Nest Group, Inc.

Pilot Columns - References• Abbott• Aventis• Baxter Hyland Immuno AG• Bayer AG• Biotest• Clariant• Genetics Institute • Genzyme • Honeywell• Novartis AG • Schering AG• Roche Diagnostics• etc …