kyle rafferty biomedical engineering. overview what are ctc’s? why is detection important? ...
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Kyle RaffertyBiomedical Engineering
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Overview
What are CTC’s? Why is detection important? Older methods of detection
Immunomagnetic beadsFlat silicon substrates
New AdvancementSilicon nanopillar substrate
Sources
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CTC’s Circulating Tumor Cells Metastasis Deadly
Cancer becoming biggest killer in world (WHO)Most common death from metastasis
Helpful to know amount in bloodstream Examining cancer metastasisPredicting patient prognosisMonitoring cancer
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Previous Methods
Immunomagnetic Beads “Utilizes capture-agent-coated magnetic beads to
immunologically recognize CTCs in the blood, followed by magnetic isolation. However, these bead-based approaches are limited by their low CTC-capture yield and purity.”
Use ferrofluids(microscopic iron particles)/antibodies and magnets to extract
CellSearch
Flat Silicon Substrate1x2 cm silicon chipCoated with streptavidin and anti-EpCAM
(epithelial cell adhesion molecule antibody)
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Silicon Nanopillar Substrate Build much like flat silicon
1x2 cm silicon ship Built by lithographic tools used for computer
chips
Difference: NanopillarsDiameter~100-200nm; Length~20um
Also coated with streptavidin and anti-EpCAM (epithelial cell adhesion molecule antibody)
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SiNP Substrate cont.
Researchers found maximum cell-capture numbers were at 45 min. incubation timeAs compared to 3-4 hrs for previous method
This is more efficient and therefore would probably
cut costsPBS: Phosphate buffered
Saline. Very common
biological solution.
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Diagram: how SiNP works
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Diagram: Si vs. SiNP
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Future Use
Researchers hope to achieve 84-91% detection
Will allow faster detection of cancer’s Allow Doctor’s to customize treatment
plan Increase early detection and survival
rates
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Sources Wang, Shutao. “Three-Dimensional Nanostructured Substrates
toward Efficient Capture of Circulating Tumor Cells.” Angewandte Chemie International Edition 48 (2009): 8970-8973. 26 Jan. 2010. http://www3.interscience.wiley.com/cgi-bin/fulltext/122658940/PDFSTART (impact factor: 10.879)
(2) Berger, Michael. “Nanotechnology trap captures cancer cells in blood.” Nanowerk, Nov. 30 2009. 26 Jan. 2010. http://www.nanowerk.com/spotlight/spotid=13743.php
Wikipedia contributors. "Metastasis." Wikipedia, The Free Encyclopedia. Wikipedia, The Free Encyclopedia, 23 Jan. 2010. Web. 26 Jan. 2010.
Stobbe, Mike. “Cancer top killer in world by 2010.” The Seattle Times, 10 Dec. 2008. 26 Jan. 2010. http://seattletimes.nwsource.com/html/health/2008489006_cancer10.html