lab number 4 blood film

8/13/2019 Lab Number 4 Blood Film

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Preparation, staining and

examination of blood film

M. Sc. Program

541 CLSLab-4-


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Blood film

Blood film should be made on clean

glass slides.

Films may be spread by hand or byautomated slid-spreader.

Blood film prepared from fresh blood(EDTA).


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STEPS FOR BLOOD FILM


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The shape of blood film


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Staining of blood films Romanowsky stains are universally employed for routine

staining of blood films. It depends on two components, namely, azure

B(trimethylthionin) and eosine Y(tetrabromo-fluorescein).

The original Romanowsky combination was polychrome

methylene blue and eosine. The presence of basic grouping on the haemoglobin molecule

results in its affinity for acidic dyes and its staining by eosin.The nucleic acids uptake of the basic dye azure B.

Granules in cytoplasm of neutrophil are weekly stained by the

azure complexes( eosinophilic granules-alkalin groupingwhich stains with acidic component of dye wherase basophilicgranules which has an affinity for the basic component ofdye).


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Types of Romanowsky stains

Leishmansstain

May-Grunwald stain

Jennersstain

Giemsasstain

Jenner-Giemsa May-Grunwald-Giemsa


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Red cell morphology

In health, the red cells have round smoothcontours and have diameters of 6.0 to8.5m.

Abnormalerythropoiesis: Anisocytosis (size: micro or macro).

Poikilocytosis (shape)

Basophilic stippling

Acanthocytosis, target cell and sicklecell.


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Erythrocyte inclusion (Howell-

jolly bodies, Pappenheimer bodies

According HB: hypochromatic

and hyperchromatic.

Spherocytosis, elliptocytosis (cellmembrane of red cell).

lab number 4 blood film

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