lab safety biology112/111
TRANSCRIPT
General Safety Rules
1. READ & FOLLOW instructions carefully before attempting
to do anything.2. Wear GOGGLES to protect
your eyes from chemicals, heated materials, or things that might be able to shatter.
3. TELL TEACHER if any spills or accidents occur.
General Safety Rules
4. After handling chemicals, always WASH YOUR HANDS with
soap and water.5. During lab work, keep your
HANDS away from your face.6. Tie back LONG hair.
General Safety Rules
7. Roll up LONG sleeves.8. Know the LOCATION of the fire
extinguisher, fire blanket, eyewash station, and first aid kit.
9. Keep your work area UNCLUTTERED. Take to the lab station only what is necessary.
General Safety Rules
10. It is suggested that you wear GLASSES rather than contact lenses.
11. Never put anything into your MOUTH during a lab experiment.
12. CLEAN your lab area at the conclusion of the laboratory period.
13. NEVER “horse around” or play practical jokes in the laboratory.
Glassware Safety
1. Chipped or cracked GLASSWARE should not be used. Show it to the teacher.
2. BROKEN glassware should not be disposed of in a classroom
trashcan. There is a special glass disposal container for it under the sink. Use Broom & DUSTPAN
Chemical Safety
1. Wear protective GLOVES and a lab apron whenever heating or pouring hazardous chemicals.
2. Never MIX two chemicals together unless the teacher the teacher directs you to.
Chemical Safety
3. If you need to smell the odor of a chemical, WAFT the fumes toward your nose with one hand. Do not put your nose over the container and inhale the fumes.
Chemical Safety
4. Follow the INSTRUCTIONS of your teacher when disposing
of all chemicals.5. WASH your hands after
handling hazardous chemicals.
Electrical Safety
1. Lay POWER cords where no one can trip on them or get caught in them.
2. Be sure your hands and your lab area are dry before using electrical equipment.
3. Never poke anything into electrical
SOCKETS.
Electrical Safety
4. Unplug cords by pulling the PLUG and not the cord.
5. Unplug all electrical equipment at the END of the lab period.
Heating Safety
1. Let burners and hotplates cool down before touching them. Perform the SIZZLE TEST to see if they are cool enough.
2. Use tongs and/or protective GLOVESto handle hot
objects.3. Never reach across an OPEN
flame or burner.
First Aid
Injury: BurnsWhat To Do: Immediately flush with
COLD water until burning sensation
is lessened.
First Aid
Injury: Cuts, bruisesWhat To Do: Do not touch an open
wound without safety gloves. Pressing directly on minor cuts will STOP bleeding in a few minutes. Apply cold compress to bruises to
reduce swelling.
First Aid
Injury: FaintingTo Do: Provide FRESH air and
have the person recline so that their head is LOWER than the rest of their body.
First Aid
Injury: EyesWhat To Do: Flush eyes immediately
with plenty of water (15 MINUTES at the eyewash). If a foreign object is lodged in the eye, do not allow the eye to be rubbed.
First Aid
Injury: PoisoningWhat To Do: Find out what substance
was responsible for the poisoning and alert the TEACHER immediately.
Fires can either be extinguished by smothering the flames with a fire blanket or clothing OR a fire extinguisher!
To use a fire extinguisher properly REMEMBER: P-A-S-S!
PULL the PINAIM at the BASESQUEEZE the TriggerSWEEP side to side
FIRE SAFETY
Always use the right type of fire
extinguisher for each specific type of fire.
*LHHS extinguishers (ABC type)
CLASS A Fires: Fires of organic matter that will leave
ashes Example: WOOD, paper, tissues
CLASS B FIRES:Fires of flammable LIQUIDSExamples: Gasoline, solvents
NOTE:Water is not a good extinguisher
because it spreads the liquid around.
CLASS C FIRES:Fires of an ELECTRICAL nature
EXAMPLES: Computers, photocopiers
WHMISWorkplace Hazardous Materials Information Systems
The 3 components to the WHMIS system are:
1. MSDS2. Worker Education3. Labels on all Products
MSDSMaterial SAFETY Data Sheet
Stored in binders in lab areasAll known chemicals8 sections (name, ingredients, physical data,
fire hazards, reactivity data, toxicological properties, preventive measures, first aid measures)
WHMIS Labels – Workplace Hazardous Materials Information System
1. Compressed GAS ex. Helium, propane2. Flammable/Combustible ex: methane
gas, acetone3. Oxidizing Material ex: Chromic Acid4. Poisonous Infectious (IMMEDIATE &
Serious Toxic Effects) ex: cyanide5. Poisonous Infectious (Other Toxic
Effects) ex: mercury, lead6. Biohazardous Infectious ex: NEEDLES7. Corrosive Materials ex: degreaser8. Dangerously Reactive ex: pure sodium
HHPS SymbolsHazardous Household Product Symbols
HOUSEHOLD!Levels of Severity (Danger, Warning and
Caution)
Precautions related to Lab:
Work in a well lit, well ventilated area Keep work area clean Properly organize work area and keep
uncluttered The smell of formaldehyde is difficult to
remove from belongings Carry microscopes properly, supporting
the base and arm Know where MSDS sheets are located
Look at the MSDS sheet: Formaldehyde Note: some specimens may be vacuum
packed
Precautions related to Dissections:
When working with preserved specimens be in a well-ventilated area
Wear vinyl gloves at all times To reduce the irritating effects of chemical
preservatives, rinse specimen under running water to eliminate excess and dilute what remains
Review procedure for cuts and punctures from dissecting tools
When cleaning dissecting instruments, hold sharp edges away from you.
More Precautions Related to Dissections:
Properly mount specimen in dissecting pan Never remove specimen or specimen parts
from the lab Clearly label and store specimens in
designated area Always ask the teacher re: proper
disposal of specimens…special containers for sharps, animal tissue and preservative disposal
When working with bacteriologic specimens:
If you have open wounds or cuts on your hands, cover them with a bandage or wear latex gloves.
Disinfect lab bench tops before and after handling bacterial cultures.
While handling bacteria, keep your hands away from your eyes, nose, and mouth.
Wash your hands thoroughly after handling bacterial
If a culture is spilled, cover the spill area with paper towel and flood the paper towels with disinfectant. Wait 20 minutes and then wipe up the spill with more paper towels and dispose of the paper towel in a empty bag.
Acute Effect: An adverse effect on any living organism which results in severe symptoms that develop rapidly; symptoms often subside after the exposure stops.
Chronic Effect: An adverse effect on a human or animal in which symptoms recur frequently or develop slowly over a long period of time.