Laboratory of Bioenergetics

main laboratory : 3.2.11, 3.2.11a

climatic chamber : 3.2.11

animal rooms: 3.0.xx

administrator: Paweł Koteja

Evolutionary Physiology Research Team

Respirometric system Sable TR3Main location: 3.2.11

• oxygen analyzer - „Oxzilla”: - 2-channel - 0-100%, res. 0.001%;

• CO2 analyzer - CA2A - 0-10%, res. 0.0001%;

• continuous measurement on 1 or 2 ch.

• sequential on 8-16 ch.

• open flow-through or semi-closed system;

• from microorganism cultures (as Microoxymax) to large animals;

• a small climatic chamber;

• mobile

Example of an open-flow setup:measurement of the maximum exercise-induced rate of aerobic metabolism

Respirometric system Sable TR3Main location: 3.2.11

Example of an open-flow setup:measurement of the maximum exercise-induced rate of aerobic metabolism

Respirometric system Sable TR3Main location: 3.2.11

More devices for respirometric measurements

• O2 analyzer Applied Electrochemistry (AMETEK) S3/AII: 2-channel, 0-100%, res. 0.001%, very fast, stable, lab. use only

• 2 x O2 Sable analyzer FC-10A: 1-channel: 0-100%, res. 0.001%, slow, not stable, mobile, 12V

• CO2 analyzer Sable CA10A: 1-channel, 0-10%, res. 0.0001%, fast, stable, mobile, 12V

• CO2 and H2O analyzer LiCor LI-840A: Low cost – "two in one", mobile, parameters similar to CA10A

• Several pumps, mass flow controllers, channel multiplexers, air driers, etc.

Motorized treadmillfor respirometric measurements

• mouse-sized animals;

• speed up to 6 km/h;

• computer controlled speed regulation;

• manual angle control

• waterproof

Activity detectors – passive infraredCustom made: 3M – Mouse Motion Monitor

• 64 sensors;• expandable;• elastic system of

data acquisition• time resolution

0.01 sec.• mobile – animal

rooms, climatic chambers

Activity detectors - mechanicalSable MAD- 1 ("seismographs")

• 8 sensors;• elastic system of

data acquisition• time resolution

0.01 sec.• mobile – animal

rooms, climatic chambers

Video system for behavioral analysesNoldus Ethovision

• video cameras, multichannel recorders• analog-digital converter ("frame grabber")• video analysis system• main result – path of animal movement• detection of a few individuals in one arena• detection in several arenas (with one individual in each);• several characteristics of movement: speed, time in particular

zones, "meandering";• automated trials for standard behavioral tests:

• open field• Morris water maze• multi-arm labyrinths

Automatic extraction apparatusBüchi B-811

• 4-positions• temperature regulation up to

+150C• extraction in neutral gas• automated:

• phases of the process (extraction, rinsing, drying)

• various types of extraction• programmable sequences

Automatic extraction apparatusBüchi B-811Types of process

Hydrolysis apparatus Büchi B-811

• 4 positions• vials compatible with the

extraction apparatus