Allele Biotech-Introducing Cost Effectiveness to Research L acZ expressing retroviral particles are the perfect companion for your viral gene delivery experiments. This retrovirus is intended to be used as an infection marker. Cells that express detectable levels of β-galactosidase within 2 days indicate a successful LacZ encoding viral infection. Allele’s LacZ Retrovirus Control is based on the Phoenix2 system, one of the most time-tested retrovirus systems in use in over 3,000 laboratories worldwide for gene delivery . Viral Particles are packaged with a proprietary process to achieve unparalleled high titer and are ready for incubation with target cells, allowing for expression of the LacZ gene. Expression Ready LacZ Retrovirus Controls Box 1 | Product Info Vector Name Cat. No. pCHAC-LacZ ABP-RP-VIRELACZL Contents 5 vials, 10 7 TU in 0.1ml per vial Protocols Storage: -80°C. Avoid repeated freeze thaw cycles Steps: 1. Plate target cells to 40-60% conuence in a 24-well plate before infection. 2. Place LacZ-virus stock (virus titer: 1x10 8 IU/ml) in a 37°C water bath and before totally thaw transfer onto ice immediately. 3. Make a serial dilution of 3-4 different multiplicity of infection (MOI = infectious units/ cell number) to test the target cells. Example 1x10 4 TE671 cells in each well of a 24-well plate: 10MOI 20MOI 30MOI 60MOI Culture Medium ( ul): 198 197 195 189 Polybrene (2mg/ml): 1 1 1 1 Virus (ul): 1 2 4 10 T otal volume (ul): 200 200 200 200 4. Tilt the plate gently every 30 min. After 3hrs, add 1 ml fresh medium. 5. After 48-72 hrs, evaluate β-galactosidase levels. 6. Choose the most efcient MOI with the least toxicity for iPS cell derivation. ♦Phoenix2 pCHAC Retroviral Vectors - For Gene Expression - For shRNA Expression - For miRNA Expression - mWasabiGFP Control - mTFP-1 Control ♦Gryphon Retroviral Packaging Cell Lines - Ampho Cell - Eco Cell ♦Gryphon Packaging Cell Selection Medium ♦Phoenix2 Hexamethrine Bromide for viral infection enhancement Related Products F or Research Use Only. Not for Diagnostic orTherapeutic Use. Purchase does not include or carry any right to resell ortransfer this product either as a stand-alone product or as a component of another product. Any use of this product other than the permitted use without the express written authorization of Allele Biotech is strictly prohibited S afety Issues: Retroviral vectors should be handled using NIH BSL-2 safety guidelines. For more information, please see Biosafety in Microbiological and Biomedical Laboratories (4 th edition) which is available on the Web sites of the National Institutes of Health at http://bmbl.od.nih.gov. Box 2 | Viral Expression Overview Plate Cells Transduction with Retroviral ParticlesIncubate and Analyze Cells after 2-3 days 18 hrs 48-72 hrs
