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Laser microdissection case study MCB 7300

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Laser microdissection case study. MCB 7300. Laser-Capture Microdissection , a Tool for the Global Analysis of Gene Expression in Specific Plant Cell Types: Identification of Genes Expressed Differentially in Epidermal Cells or Vascular Tissues of Maize. - PowerPoint PPT Presentation

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Page 1: Laser microdissection case study

Laser microdissection case study

MCB 7300

Page 2: Laser microdissection case study

Laser-Capture Microdissection, a Tool for the Global Analysis of Gene

Expression in Specific Plant Cell Types:

Identification of Genes Expressed Differentially in Epidermal Cells or

Vascular Tissues of Maize

Mikio Nakazono, Fang Qiu, Lisa A. Borsuk and Patrick S. SchnableThe Plant Cell, Vol. 15, No. 3 (Mar., 2003), pp. 583-596

Page 3: Laser microdissection case study

Overview

• RNA isolation from different plant cell types for microarray analysis

• Protocol changes: cryoprotectant; coagulating fixative (ethanol:acetic acid)

Page 4: Laser microdissection case study
Page 5: Laser microdissection case study

Yields

Page 6: Laser microdissection case study

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