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Author(s): Groza, M (Groza, Marinela); Jitaru, D (Jitaru, Daniela); Badescu, L (Badescu, Laurentiu); Ciocoiu, M (Ciocoiu, Manuela); Badescu, M(Badescu, Magda)

Title: Evaluation of the immune defense in diabetes mellitus using an experimental model

Source: ROMANIAN BIOTECHNOLOGICAL LETTERS, 16 (1): 5971-5979 JAN-FEB 2011

Language: EnglishDocument Type: Article

Author Keywords: Streptozotocin-induced diabetes; Aronia melanocarpa; Sambucus nigra

KeyWords Plus: T-CELLS

Abstract: Streptozotocin-induced diabetes in Wistar rats can be considered a good experimental model for the study of the type I diabetes mellitus(insulin-dependent). It is also known that in die context of this illness the metabolism of the whole body is disrupted, as the oxidative stress is major and the deficient immune defense is associated with abnormalities in the circulating lymphocyte subtypes and the autoantibodies. In this s tudy, wehave evaluated wi th the help of flow cytometry the population of T CD3+ lymphocytes out of which CD4+ cells with the CD45RA/RC+ and CD8a+

phenotypes in Wistar rats with Streptozotocin-induced diabetes: the groups under study received or not extracts from natural substances such asAronia melanocarpa and Sambucus nigra. The results have been compared to the ones obtained for the lot of witness healthy rats. The administrationof certain substances with a protective role (Aronia melanocarpa, Sambucus nigra) to the lot of diabetic rats leads to a significant increase in thenumber of T CD3+ cells (p < 0.001) as well as in the T helper CD4+ cells (p < 0.001), but also in the production of T helper naive CD4+CD45RA+lymphocytes (p < 0.001), respectively a decrease in the population of T CD4+CD45RC+ cells, especially for the DM+A group in comparison to the

lot of untreated diabetic rats (DM). All these results show that the immune defense in diabetes mellitus on experimental animal model can besignificantly improved by the administration of natural substances such as Aronia melanocarpa, respectively Sambucus nigra.

Addresses: [Groza, Marinela; Ciocoiu, Manuela; Badescu, Magda] Gr T Popa Univ Med & Pharm, Physiopathol Dept, Iafi, Romania; [Jitaru,Daniela] St Spir idon Hosp, Lab Immunol & Genet, Iafi, Romania; [Badescu, Laurentiu] Gr T Popa Univ Med & Pharm, Cel l & Mol Biol Dept, Iafi,Romania

Reprint Address: Badescu, M, UMF Iasi, Dept Pathophysiol, 16 Univ St, Iafi 700115, Romania.

E-mail Address : magda.badescu@gmail.com

Cited Reference Count: 12

Times Cited: 0

Publisher: ARS DOCENDI

Publisher Address: SOS PANDURI 90, SECT 5, BUCHAREST, RO-050663, ROMANIA

ISSN: 1224-5984

29-char Source Abbrev.: ROM BIOTECH LETTISO Source Abbrev.: Rom. Biotech. Lett.

Source Item Page Count: 9

Subject Category: Biotechnology & Applied Microbiology

ISI Document Delivery No.: 729ZS

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