S244 Late Breaking Abstracts

the BHSCT. As a result a significant proportion of the dieteticcaseload consisted of patients from this NH, reducing capacityto see patients in other settings.Methods: The dietetic service met with NH management todiscuss the nutritional management of the ’at risk’ patientsand agreed to implement a ‘food first’ approach. An actionplan containing specific recommendations with regards toa highly fortified, nutritious diet for patients at risk ofmalnutrition was agreed. The NH created a food fortificationrecord to be completed by all members of staff on a dailybasis.Results: Referrals: After 4 months of implementation of a‘food first’ approach, referrals to the dietetic service fromthis NH had reduced by 75%.Nutritional Outcome: Prior to the project, 61% of patients onthe dietetic caseload residing in this NH lost weight despitebeing on oral nutritional supplements. Four months on, 100%patients either maintained or gained weight with no recentweight loss reported.Conclusion: A dietitian monitors the NH to ensure foodfortification and reduced referral rates are sustained. If thisproject was implemented within all NHs in the BHSCT area,there could potentially be a 75% reduction in NH referralsto CNDS. Saving in health care costs will be realised throughincreased capacity to effectively manage a wider cohort ofadults at risk of, or with malnutrition.

Disclosure of Interest: None Declared.

LB014-SUNGLUTAMIC ACID HAS A LIVER-PROTECTIVE EFFECTTHROUGH THE SUPPRESSION OF INDUCIBLE NITRIC OXIDESYNTHASER. Nakatake1, T. Okumura1,2, H. Miki1, Y. Ueyama1, T. Tsuda1,Y. Nakamura1, K. Tokuhara1, M. Kaibori1, M. Nishizawa3,A-H. Kwon1. 1Department of Surgery, Kansai MedicalUniversity, Osaka, 2Research Organization of Scienceand Technology, Ritsumeikan University, 3Department ofBiomedical Sciences, College of Life Sciences, RitsumeikanUniversity, Shiga, Japan

Rationale: Amino acids are reportedly used for pre- andpost-operation treatments in patients with digestive diseases.Glutamic acid (Glu) is a precursor of glutathione, which isresponsible for the antioxidative response inside the body,and its supplementation can contribute to the biosynthesisof glutathione. In inflamed liver, proinflammatory cytokinessuch as TNF-a and interleukin (IL)-1b, stimulate liver cells(Kupffer cells and hepatocytes), followed by the induction ofinducible nitric oxide synthase (iNOS) and production of nitricoxide (NO). Excessive amount of NO production by iNOS hasbeen implicated as one of the factors in liver injury. So it isessential to inhibit iNOS induction for the prevention of liverinjury. In this study, we examined proinflammatory cytokine-stimulated hepatocytes as a simple “in vitro injury model” toinvestigate liver protective effects of Glu.Methods: Hepatocytes were isolated from rats (200 g) andcultured. Cells were treated with IL-1b in the presence orabsence of Glu. The induction of NO production and iNOS,and its signaling pathway were analyzed.Results: Proinflammatory cytokine IL-1b stimulates the in-duction of iNOS gene expression, which is followed by

increased production of NO, in primary cultures of rathepatocytes. The simultaneous addition of Glu and IL-1bdecreased the production of NO and the expression ofiNOS protein dose- and time-dependently. Glu (1 10 mg/ml)showed no cellular cytotoxicity as evaluated by the releaseof lactate dehydrogenase into the culture medium and Trypanblue exclusion in hepatocytes. Electrophoretic mobility shiftassay with the nuclear extracts revealed that Glu inhibitedthe translocation of NF-úB to the nucleus and its DNA binding.Conclusion: These findings demonstrate that Glu inhibits iNOSgene expression through the inhibition of NF-úB pathway,leading to the reduction of NO production. Glu may havetherapeutic potential for organ injuries including liver.

Disclosure of Interest: None Declared.

LB015-SUNPREVENTION OF OBESITY USING KETOGENICMEDITERRANEAN DIET AND MEDITERRANEAN DIETMAINTENANCE PROTOCOLM. Vranceanu1, M. Marchetti2, S. Barrucco3. 1OPMCA,Bucharest, Romania; 2Farmacia, 3Universita Tor Vergata,Rome, Italy

Rationale: There are many types of diet but only protein diethas been used successfully to prevent loss of lean body massin obese patients. We studied obese patients receiving shorttreatments of an exclusive protein-based nutritional solutionin a first cycle and protein plus two servings (protein andvegetables) in a second cycle.Methods: There were 212 patients in the study with agebetween 31 and 55 years old. Women:men ratio 2:4. Initialbody mass index 38.3±8.1. At the onset of treatment, averagefat mass was 47.8±14.5 kg while BCM was 43.2±6.8 kg inmales and 26.3±3.9 kg in females. The body composition waschecked with a BIA.All patients underwent 10-day cycles with a nutritionalsolution consisted solely of 50 65 g of proteins, we usedamin 21k plus vitamins and Q10dietary supplement.In the second cycle were added 2 servings (lunch and dinner)with protein and vegetables and we calculated daily proteinrequirement. In each cycle the intake protein was normal(between 1.2 and 1.5 g/kg).Results: After treatment the patients lost 10.2±7.0 kg of bodyweight, 5.8±5.5 kg of fat mass and 2.2±3.3 kg of BCM.Conclusion: Ketogenic nutrition treatment with amin 21kinduced a rapid 10% weight loss, 57% of which was Fat Mass.

Disclosure of Interest: None Declared.

LB016-SUNNUTRITIONAL STATUS OF CHILDREN ADMITTED IN ADIGESTIVE ENDOSCOPY UNIT, ASSESSED BY INTERNATIONALGROWTH CHARTSV. Hurduc1, L. Bordei1, M. Iancu1, E. Buzoianu1, D.A. Plesca1.1Pediatric Gastroenterology, Victor Gomoiu ClinicalHospital, Carol Davila University of Medicine And Pharmacy,Bucharest, Romania

Rationale: Our country is in a phase of nutritional transitionand has not updated its growth charts in the last decades.Due to this reason, the children’s nutritional status is mainlyassessed by international growth references. The aim of this