lc12502 - sigmaaldrich.com
TRANSCRIPT
Certi�icateofAnalysis1,2,4-BUTANETRIOL,INTERNALSTD#1,1X5ML,1000UG/ML,PYRIDINE
Certi�ied
Reference
Material
DescriptionProductID CRM44896
Lot LRAC3547
July2021ExpirationDate
ManufacturingDate July2019
REFRIGERATEStorageConditions
Solvent/Matrix PYRIDINE
Certi�iedValues
RawMaterial
Purity,%
RawMaterial
LotUnitsAnalyte
1,4Certi�ied
ValueCAS
Elution
order
1,2,4-BUTANETRIOL µg/ml 42890-76-699.7 2 LC125021000 ± 18
Additional Information:AnalyticalMethodParameters:
Column:SPB-530mx0.53mmx1.5um
40°Cto250°C@15°C/min
Detector:FID,310°CDerivatizedwithBSTFA&TMCS
InjectionVolume:1µl
Page 1 of 2
2931 Soldier Springs Rd. Laramie, Wyoming 82070 [email protected] www.sigma-aldrich.com
DescriptionLot LRAC3547
July2021ExpirationDate
ManufacturingDate July2019
REFRIGERATEStorageConditions
Solvent/Matrix PYRIDINE
0-8302019
AndyOmmen-QCManager MarkPooler-QASupervisor
Certi�icationDate
Version
1 Metrological traceability: Traceable to the SI and higher order standards from NIST through an unbroken chain of comparisons. The balance used to weigh raw materials is accurate to +/-0.0001 g and
calibrated regularly using mass standards traceable to NIST. All dilutions were performed gravimetrically. Additionally, individual analytes are traceable to NIST SRMs where available and specified above.4 Ucrm - Uncertainty values in this document are expressed as Expanded Uncertainty (Ucrm) corresponding to the 95% confidence interval. Ucrm is derived from the combined standard uncertainty multiplied by the coverage factor k, which is obtained from a t-distribution and degrees of freedom. The components of combined standard uncertainty include the uncertainties due to characterization, homogeneity, long term stability, and short term stability (transport). The components due to stability are generally considered to be negligible unless otherwise indicated by stability studies. The mathematical representation of the Ucrm calculation is as follows:
k: Coverage factor derived from a t-distribution table, based on the degrees of freedom of the data set. Assume 2.0 for a Confidence interval = 95%
6 Analytical Value- For QC verification of the certified value only- not to be used in calculations. Represents the analytical data obtained by comparison to a standard as analyzed by the method described in the CoA or another acceptable method. The result may differ from the certified value and UCRM based on method uncertainty as well as the uncertainty associated with the standard used for comparison.
Traceability: The standard was manufactured under an ISO/IEC 17025:2017 certified quality system. The balance used to weigh raw materials is accurate to +/- 0.0001g and calibrated regularly using mass standards traceable to NIST. All dilutions were preformed gravimetrically. Additionally, individual analytes are traceable to NIST SRMs where available and specified above.
Homogeneity: Homogeneity was assessed in accordance with ISO 17034:2016. Completed units were sampled using a random stratified sampling protocol. The results of chemical analysis were then compared using a one-way analysis of variance approach as described by TNI EL-V3-2009 Appendix A.2. See Instructions for minimum sub-sample size.
Expiration is at end of month given on certificate and label.THIS PRODUCT WAS DESIGNED, PRODUCED AND VERIFIED FOR ACCURACY AND STABILITY IN ACCORDANCE WITH ISOISOISOISO////IEC IEC IEC IEC 17025170251702517025::::2017 2017 2017 2017 ((((ANAB Cert ATANAB Cert ATANAB Cert ATANAB Cert AT----1467146714671467) ) ) ) and ISO ISO ISO ISO 17034170341703417034::::2016 2016 2016 2016 ((((ANAB Cert ARANAB Cert ARANAB Cert ARANAB Cert AR----1470147014701470).).).).MSDS reports for components comprising greater than 1.0% of the solution or 0.1% for components known to be carcinogens are available upon request.August30,2019
22
hom
2
stabilityogeneitycharCRM uuuu ++=
Page 2 of 2
Certi�icateofAnalysisTRICAPRININTERNALSTANDARD#2,1X5ML,8000UG/ML,PYRIDINE
Certi�ied
Reference
Material
DescriptionProductID CRM44897
Lot LRAC3544
July2021ExpirationDate
ManufacturingDate July2019
RefrigerateStorageConditions
Solvent/Matrix PYRIDINE
Certi�iedValues
RawMaterial
Purity,%
RawMaterial
LotUnitsAnalyte
1,4Certi�ied
ValueCAS
Elution
order
Tricaprin µg/ml 621-71-6100 1 SLBV30938004 ± 393
Additional Information:AnalyticalMethodParameters:
Column:SP-2560,100mx0.25mmx0.2µmdf,Flow:1.5ml/min
Inlet:240oC,SplitRatio:20:1
80oC(1min)to220oC(4min)@20oC/min,to250oC(3min)@10oC/min
Detector:FID,260oC
InjectionVolume:1.0µl
Page 1 of 2
2931 Soldier Springs Rd. Laramie, Wyoming 82070 [email protected] www.sigma-aldrich.com
DescriptionLot LRAC3544
July2021ExpirationDate
ManufacturingDate July2019
RefrigerateStorageConditions
Solvent/Matrix PYRIDINE
0-8302019
AndyOmmen-QCManager MarkPooler-QASupervisor
Certi�icationDate
Version
1 Metrological traceability: Traceable to the SI and higher order standards from NIST through an unbroken chain of comparisons. The balance used to weigh raw materials is accurate to +/-0.0001 g and
calibrated regularly using mass standards traceable to NIST. All dilutions were performed gravimetrically. Additionally, individual analytes are traceable to NIST SRMs where available and specified above.4 Ucrm - Uncertainty values in this document are expressed as Expanded Uncertainty (Ucrm) corresponding to the 95% confidence interval. Ucrm is derived from the combined standard uncertainty multiplied by the coverage factor k, which is obtained from a t-distribution and degrees of freedom. The components of combined standard uncertainty include the uncertainties due to characterization, homogeneity, long term stability, and short term stability (transport). The components due to stability are generally considered to be negligible unless otherwise indicated by stability studies. The mathematical representation of the Ucrm calculation is as follows:
k: Coverage factor derived from a t-distribution table, based on the degrees of freedom of the data set. Assume 2.0 for a Confidence interval = 95%
6 Analytical Value- For QC verification of the certified value only- not to be used in calculations. Represents the analytical data obtained by comparison to a standard as analyzed by the method described in the CoA or another acceptable method. The result may differ from the certified value and UCRM based on method uncertainty as well as the uncertainty associated with the standard used for comparison.
Traceability: The standard was manufactured under an ISO/IEC 17025:2017 certified quality system. The balance used to weigh raw materials is accurate to +/- 0.0001g and calibrated regularly using mass standards traceable to NIST. All dilutions were preformed gravimetrically. Additionally, individual analytes are traceable to NIST SRMs where available and specified above.
Homogeneity: Homogeneity was assessed in accordance with ISO 17034:2016. Completed units were sampled using a random stratified sampling protocol. The results of chemical analysis were then compared using a one-way analysis of variance approach as described by TNI EL-V3-2009 Appendix A.2. See Instructions for minimum sub-sample size.
Expiration is at end of month given on certificate and label.THIS PRODUCT WAS DESIGNED, PRODUCED AND VERIFIED FOR ACCURACY AND STABILITY IN ACCORDANCE WITH ISOISOISOISO////IEC IEC IEC IEC 17025170251702517025::::2017 2017 2017 2017 ((((ANAB Cert ATANAB Cert ATANAB Cert ATANAB Cert AT----1467146714671467) ) ) ) and ISO ISO ISO ISO 17034170341703417034::::2016 2016 2016 2016 ((((ANAB Cert ARANAB Cert ARANAB Cert ARANAB Cert AR----1470147014701470).).).).MSDS reports for components comprising greater than 1.0% of the solution or 0.1% for components known to be carcinogens are available upon request.August30,2019
22
hom
2
stabilityogeneitycharCRM uuuu ++=
Page 2 of 2
Certi�icateofAnalysisASTMD6584STANDARDSOLUTION1,1X1ML,VARIED,PYRIDINE
Certi�ied
Reference
Material
DescriptionProductID CRM44899
Lot LRAC3548
July2021ExpirationDate
ManufacturingDate July2019
RefrigerateStorageConditions
Solvent/Matrix PYRIDINE
Certi�iedValues
RawMaterial
Purity,%
RawMaterial
LotUnitsAnalyte
1,4Certi�ied
ValueCAS
Elution
orderAnalytical
Value
6
5.0GLYCEROL µg/ml 56-81-599.4 1 LB793165.0 ± 0.77
96.81-MONOOLEOYL-RAC-GLYCEROL µg/ml 111-03-599.9 3 LC1642299.7 ± 23
50.11,3-DIOLEIN µg/ml 2465-32-999.9 5 LC1586350.0 ± 16
47.4TRIOLEIN µg/ml 122-32-799.9 6 LC1480150.0 ± 14
Additional Information:AnalyticalMethodParameters:
Column:MET-BIODIESEL16mx0.53mmx0.16µmID
50°Cfor1min.Then15°C/minto180°C.Then7°C/minto
230°C.Then30°C/minto370°Cfor10min.
Detector:FID;380°C
InjectionVolume:1µl
Page 1 of 2
2931 Soldier Springs Rd. Laramie, Wyoming 82070 [email protected] www.sigma-aldrich.com
DescriptionLot LRAC3548
July2021ExpirationDate
ManufacturingDate July2019
RefrigerateStorageConditions
Solvent/Matrix PYRIDINE
0-8302019
AndyOmmen-QCManager MarkPooler-QASupervisor
Certi�icationDate
Version
1 Metrological traceability: Traceable to the SI and higher order standards from NIST through an unbroken chain of comparisons. The balance used to weigh raw materials is accurate to +/-0.0001 g and
calibrated regularly using mass standards traceable to NIST. All dilutions were performed gravimetrically. Additionally, individual analytes are traceable to NIST SRMs where available and specified above.4 Ucrm - Uncertainty values in this document are expressed as Expanded Uncertainty (Ucrm) corresponding to the 95% confidence interval. Ucrm is derived from the combined standard uncertainty multiplied by the coverage factor k, which is obtained from a t-distribution and degrees of freedom. The components of combined standard uncertainty include the uncertainties due to characterization, homogeneity, long term stability, and short term stability (transport). The components due to stability are generally considered to be negligible unless otherwise indicated by stability studies. The mathematical representation of the Ucrm calculation is as follows:
k: Coverage factor derived from a t-distribution table, based on the degrees of freedom of the data set. Assume 2.0 for a Confidence interval = 95%
6 Analytical Value- For QC verification of the certified value only- not to be used in calculations. Represents the analytical data obtained by comparison to a standard as analyzed by the method described in the CoA or another acceptable method. The result may differ from the certified value and UCRM based on method uncertainty as well as the uncertainty associated with the standard used for comparison.
Traceability: The standard was manufactured under an ISO/IEC 17025:2017 certified quality system. The balance used to weigh raw materials is accurate to +/- 0.0001g and calibrated regularly using mass standards traceable to NIST. All dilutions were preformed gravimetrically. Additionally, individual analytes are traceable to NIST SRMs where available and specified above.
Homogeneity: Homogeneity was assessed in accordance with ISO 17034:2016. Completed units were sampled using a random stratified sampling protocol. The results of chemical analysis were then compared using a one-way analysis of variance approach as described by TNI EL-V3-2009 Appendix A.2. See Instructions for minimum sub-sample size.
Expiration is at end of month given on certificate and label.THIS PRODUCT WAS DESIGNED, PRODUCED AND VERIFIED FOR ACCURACY AND STABILITY IN ACCORDANCE WITH ISOISOISOISO////IEC IEC IEC IEC 17025170251702517025::::2017 2017 2017 2017 ((((ANAB Cert ATANAB Cert ATANAB Cert ATANAB Cert AT----1467146714671467) ) ) ) and ISO ISO ISO ISO 17034170341703417034::::2016 2016 2016 2016 ((((ANAB Cert ARANAB Cert ARANAB Cert ARANAB Cert AR----1470147014701470).).).).MSDS reports for components comprising greater than 1.0% of the solution or 0.1% for components known to be carcinogens are available upon request.August30,2019
22
hom
2
stabilityogeneitycharCRM uuuu ++=
Page 2 of 2
Certi�icateofAnalysisASTMD6584STANDARDSOLUTION2,1X1ML,VARIED,PYRIDINE
Certi�ied
Reference
Material
DescriptionProductID CRM44914
Lot LRAC3549
July2021ExpirationDate
ManufacturingDate July2019
REFRIGERATEStorageConditions
Solvent/Matrix PYRIDINE
Certi�iedValues
RawMaterial
Purity,%
RawMaterial
LotUnitsAnalyte
1,4Certi�ied
ValueCAS
Elution
orderAnalytical
Value
6
14.9GLYCEROL µg/ml 56-81-599.4 1 LB7931615.1 ± 0.60
2601-MONOOLEOYL-RAC-GLYCEROL µg/ml 111-03-599.9 3 LC16422250 ± 11
98.51,3-DIOLEIN µg/ml 2465-32-999.9 5 LC26387100 ± 3.8
103TRIOLEIN µg/ml 122-32-799.9 6 LC14801100 ± 4.3
Additional Information:AnalyticalMethodParameters:
Column:MET-Biodieselw/int.2mguard,14mx0.53mmx0.16µmdf,Column2low:4.6mL/min
InletTemp:50°CCool-on-columninjection
50°C(2min)to180°Cat15°C/min@35°C/minto370°C(8min)
Detector:FID,380°C
InjectionVolume:0.6µL
DerivatizedwithMSTFA
(0.5mLofSAwith0.2mLofMSTFA,heatedat70°Cfor20min)
Page 1 of 2
2931 Soldier Springs Rd. Laramie, Wyoming 82070 [email protected] www.sigma-aldrich.com
DescriptionLot LRAC3549
July2021ExpirationDate
ManufacturingDate July2019
REFRIGERATEStorageConditions
Solvent/Matrix PYRIDINE
0-8302019
AndyOmmen-QCManager MarkPooler-QASupervisor
Certi�icationDate
Version
1 Metrological traceability: Traceable to the SI and higher order standards from NIST through an unbroken chain of comparisons. The balance used to weigh raw materials is accurate to +/-0.0001 g and
calibrated regularly using mass standards traceable to NIST. All dilutions were performed gravimetrically. Additionally, individual analytes are traceable to NIST SRMs where available and specified above.4 Ucrm - Uncertainty values in this document are expressed as Expanded Uncertainty (Ucrm) corresponding to the 95% confidence interval. Ucrm is derived from the combined standard uncertainty multiplied by the coverage factor k, which is obtained from a t-distribution and degrees of freedom. The components of combined standard uncertainty include the uncertainties due to characterization, homogeneity, long term stability, and short term stability (transport). The components due to stability are generally considered to be negligible unless otherwise indicated by stability studies. The mathematical representation of the Ucrm calculation is as follows:
k: Coverage factor derived from a t-distribution table, based on the degrees of freedom of the data set. Assume 2.0 for a Confidence interval = 95%
6 Analytical Value- For QC verification of the certified value only- not to be used in calculations. Represents the analytical data obtained by comparison to a standard as analyzed by the method described in the CoA or another acceptable method. The result may differ from the certified value and UCRM based on method uncertainty as well as the uncertainty associated with the standard used for comparison.
Traceability: The standard was manufactured under an ISO/IEC 17025:2017 certified quality system. The balance used to weigh raw materials is accurate to +/- 0.0001g and calibrated regularly using mass standards traceable to NIST. All dilutions were preformed gravimetrically. Additionally, individual analytes are traceable to NIST SRMs where available and specified above.
Homogeneity: Homogeneity was assessed in accordance with ISO 17034:2016. Completed units were sampled using a random stratified sampling protocol. The results of chemical analysis were then compared using a one-way analysis of variance approach as described by TNI EL-V3-2009 Appendix A.2. See Instructions for minimum sub-sample size.
Expiration is at end of month given on certificate and label.THIS PRODUCT WAS DESIGNED, PRODUCED AND VERIFIED FOR ACCURACY AND STABILITY IN ACCORDANCE WITH ISOISOISOISO////IEC IEC IEC IEC 17025170251702517025::::2017 2017 2017 2017 ((((ANAB Cert ATANAB Cert ATANAB Cert ATANAB Cert AT----1467146714671467) ) ) ) and ISO ISO ISO ISO 17034170341703417034::::2016 2016 2016 2016 ((((ANAB Cert ARANAB Cert ARANAB Cert ARANAB Cert AR----1470147014701470).).).).MSDS reports for components comprising greater than 1.0% of the solution or 0.1% for components known to be carcinogens are available upon request.August30,2019
22
hom
2
stabilityogeneitycharCRM uuuu ++=
Page 2 of 2
Certi�icateofAnalysisASTMD6584STANDARDSOLUTION3,1X1ML,VARIED,PYRIDINE
Certi�ied
Reference
Material
DescriptionProductID CRM44915
Lot LRAC3550
July2021ExpirationDate
ManufacturingDate July2019
RefrigerateStorageConditions
Solvent/Matrix PYRIDINE
Certi�iedValues
RawMaterial
Purity,%
RawMaterial
LotUnitsAnalyte
1,4Certi�ied
ValueCAS
Elution
orderAnalytical
Value
6
26.1GLYCEROL µg/ml 56-81-599.4 1 LB7931625.0 ± 2
4721-MONOOLEOYL-RAC-GLYCEROL µg/ml 111-03-599.9 2 LC16422499 ± 63
1901,3-DIOLEIN µg/ml 2465-32-9100 3 SLBV3160200 ± 7
203TRIOLEIN µg/ml 122-32-7100 4 MKBX0108V201 ± 31
Additional Information:AnalyticalMethodParameters:
Column:MET-Biodieselw/int.2mguard,14mx0.53mmx0.16µmdf,Column0low:4.5mL/min
InletTemp:40°CCool-on-columninjection
40°C(1min)to380°C(15min)at25°C/min
Detector:FID,380°C
InjectionVolume:1.0µL
Page 1 of 2
2931 Soldier Springs Rd. Laramie, Wyoming 82070 [email protected] www.sigma-aldrich.com
DescriptionLot LRAC3550
July2021ExpirationDate
ManufacturingDate July2019
RefrigerateStorageConditions
Solvent/Matrix PYRIDINE
0-8302019
AndyOmmen-QCManager MarkPooler-QASupervisor
Certi�icationDate
Version
1 Metrological traceability: Traceable to the SI and higher order standards from NIST through an unbroken chain of comparisons. The balance used to weigh raw materials is accurate to +/-0.0001 g and
calibrated regularly using mass standards traceable to NIST. All dilutions were performed gravimetrically. Additionally, individual analytes are traceable to NIST SRMs where available and specified above.4 Ucrm - Uncertainty values in this document are expressed as Expanded Uncertainty (Ucrm) corresponding to the 95% confidence interval. Ucrm is derived from the combined standard uncertainty multiplied by the coverage factor k, which is obtained from a t-distribution and degrees of freedom. The components of combined standard uncertainty include the uncertainties due to characterization, homogeneity, long term stability, and short term stability (transport). The components due to stability are generally considered to be negligible unless otherwise indicated by stability studies. The mathematical representation of the Ucrm calculation is as follows:
k: Coverage factor derived from a t-distribution table, based on the degrees of freedom of the data set. Assume 2.0 for a Confidence interval = 95%
6 Analytical Value- For QC verification of the certified value only- not to be used in calculations. Represents the analytical data obtained by comparison to a standard as analyzed by the method described in the CoA or another acceptable method. The result may differ from the certified value and UCRM based on method uncertainty as well as the uncertainty associated with the standard used for comparison.
Traceability: The standard was manufactured under an ISO/IEC 17025:2017 certified quality system. The balance used to weigh raw materials is accurate to +/- 0.0001g and calibrated regularly using mass standards traceable to NIST. All dilutions were preformed gravimetrically. Additionally, individual analytes are traceable to NIST SRMs where available and specified above.
Homogeneity: Homogeneity was assessed in accordance with ISO 17034:2016. Completed units were sampled using a random stratified sampling protocol. The results of chemical analysis were then compared using a one-way analysis of variance approach as described by TNI EL-V3-2009 Appendix A.2. See Instructions for minimum sub-sample size.
Expiration is at end of month given on certificate and label.THIS PRODUCT WAS DESIGNED, PRODUCED AND VERIFIED FOR ACCURACY AND STABILITY IN ACCORDANCE WITH ISOISOISOISO////IEC IEC IEC IEC 17025170251702517025::::2017 2017 2017 2017 ((((ANAB Cert ATANAB Cert ATANAB Cert ATANAB Cert AT----1467146714671467) ) ) ) and ISO ISO ISO ISO 17034170341703417034::::2016 2016 2016 2016 ((((ANAB Cert ARANAB Cert ARANAB Cert ARANAB Cert AR----1470147014701470).).).).MSDS reports for components comprising greater than 1.0% of the solution or 0.1% for components known to be carcinogens are available upon request.August30,2019
22
hom
2
stabilityogeneitycharCRM uuuu ++=
Page 2 of 2
Certi�icateofAnalysisASTMD6584STANDARDSOLUTION4,1X1ML,VARIED,PYRIDINE
Certi�ied
Reference
Material
DescriptionProductID CRM44916
Lot LRAC3545
July2021ExpirationDate
ManufacturingDate July2019
RefrigerateStorageConditions
Solvent/Matrix PYRIDINE
Certi�iedValues
RawMaterial
Purity,%
RawMaterial
LotUnitsAnalyte
1,4Certi�ied
ValueCAS
Elution
orderAnalytical
Value
6
38GLYCEROL µg/ml 56-81-599.4 1 LB7931635 ± 2
772MONOOLEIN µg/ml 111-03-0599.9 2 LC16422750 ± 49
3631,3-DIOLEIN µg/ml 2465-32-9100 3 SLBV3160352 ± 56
343TRIOLEIN µg/ml 122-32-7100 4 MKBX0108V351 ± 43
Additional Information:AnalyticalMethodParameters:
Column:MET-Biodieselw/int.2mguard,14mx0.53mmx0.16µmdf,Column0low:4.6mL/min
InletTemp:43°CCool-on-columninjection
50°C(3min)to180°Cat12°C/min@30°C/minto370°C(10min)
Detector:FID,380°C
InjectionVolume:0.6µL
DerivatizedwithMSTFA
(0.5mLofsamplewith0.2mLofMSTFA,heatedat70°Cfor20min)
Page 1 of 2
2931 Soldier Springs Rd. Laramie, Wyoming 82070 [email protected] www.sigma-aldrich.com
DescriptionLot LRAC3545
July2021ExpirationDate
ManufacturingDate July2019
RefrigerateStorageConditions
Solvent/Matrix PYRIDINE
0-8302019
AndyOmmen-QCManager MarkPooler-QASupervisor
Certi�icationDate
Version
1 Metrological traceability: Traceable to the SI and higher order standards from NIST through an unbroken chain of comparisons. The balance used to weigh raw materials is accurate to +/-0.0001 g and
calibrated regularly using mass standards traceable to NIST. All dilutions were performed gravimetrically. Additionally, individual analytes are traceable to NIST SRMs where available and specified above.4 Ucrm - Uncertainty values in this document are expressed as Expanded Uncertainty (Ucrm) corresponding to the 95% confidence interval. Ucrm is derived from the combined standard uncertainty multiplied by the coverage factor k, which is obtained from a t-distribution and degrees of freedom. The components of combined standard uncertainty include the uncertainties due to characterization, homogeneity, long term stability, and short term stability (transport). The components due to stability are generally considered to be negligible unless otherwise indicated by stability studies. The mathematical representation of the Ucrm calculation is as follows:
k: Coverage factor derived from a t-distribution table, based on the degrees of freedom of the data set. Assume 2.0 for a Confidence interval = 95%
6 Analytical Value- For QC verification of the certified value only- not to be used in calculations. Represents the analytical data obtained by comparison to a standard as analyzed by the method described in the CoA or another acceptable method. The result may differ from the certified value and UCRM based on method uncertainty as well as the uncertainty associated with the standard used for comparison.
Traceability: The standard was manufactured under an ISO/IEC 17025:2017 certified quality system. The balance used to weigh raw materials is accurate to +/- 0.0001g and calibrated regularly using mass standards traceable to NIST. All dilutions were preformed gravimetrically. Additionally, individual analytes are traceable to NIST SRMs where available and specified above.
Homogeneity: Homogeneity was assessed in accordance with ISO 17034:2016. Completed units were sampled using a random stratified sampling protocol. The results of chemical analysis were then compared using a one-way analysis of variance approach as described by TNI EL-V3-2009 Appendix A.2. See Instructions for minimum sub-sample size.
Expiration is at end of month given on certificate and label.THIS PRODUCT WAS DESIGNED, PRODUCED AND VERIFIED FOR ACCURACY AND STABILITY IN ACCORDANCE WITH ISOISOISOISO////IEC IEC IEC IEC 17025170251702517025::::2017 2017 2017 2017 ((((ANAB Cert ATANAB Cert ATANAB Cert ATANAB Cert AT----1467146714671467) ) ) ) and ISO ISO ISO ISO 17034170341703417034::::2016 2016 2016 2016 ((((ANAB Cert ARANAB Cert ARANAB Cert ARANAB Cert AR----1470147014701470).).).).MSDS reports for components comprising greater than 1.0% of the solution or 0.1% for components known to be carcinogens are available upon request.August30,2019
22
hom
2
stabilityogeneitycharCRM uuuu ++=
Page 2 of 2
Certi�icateofAnalysisASTMD6584STANDARDSOLUTION5,1X1ML,VARIED,PYRIDINE
Certi�ied
Reference
Material
DescriptionProductID CRM44917
Lot LRAC3546
July2021ExpirationDate
ManufacturingDate July2019
REFRIGERATEStorageConditions
Solvent/Matrix PYRIDINE
Certi�iedValues
RawMaterial
Purity,%
RawMaterial
LotUnitsAnalyte
1,4Certi�ied
ValueCAS
Elution
orderAnalytical
Value
6
53.6GLYCEROL µg/ml 56-81-599.4 1 LB7931649.9 ± 3
10351-MONOOLEOYL-RAC-GLYCEROL µg/ml 122-32-799.9 2 LC164221003 ± 23
5151,3-DIOLEIN µg/ml 111-03-5100 3 SLBV3160502 ± 21
507TRIOLEIN µg/ml 2465-32-9100 4 MKBX0108V500 ± 26
Additional Information:AnalyticalMethodParameters:
Column:MET-Biodieselw/int.2mguard,14mx0.53mmx0.16µmdf,Column3low:4.6mL/min
InletTemp:43°CCool-on-columninjection
50°C(3min)to180°Cat12°C/min@30°C/minto370°C(10min)
Detector:FID,380°C
InjectionVolume:0.6µL
DerivatizedwithMSTFA
(0.5mLofSAwith0.2mLofMSTFA,heatedat70°Cfor20min)
Page 1 of 2
2931 Soldier Springs Rd. Laramie, Wyoming 82070 [email protected] www.sigma-aldrich.com
DescriptionLot LRAC3546
July2021ExpirationDate
ManufacturingDate July2019
REFRIGERATEStorageConditions
Solvent/Matrix PYRIDINE
0-8302019
AndyOmmen-QCManager MarkPooler-QASupervisor
Certi�icationDate
Version
1 Metrological traceability: Traceable to the SI and higher order standards from NIST through an unbroken chain of comparisons. The balance used to weigh raw materials is accurate to +/-0.0001 g and
calibrated regularly using mass standards traceable to NIST. All dilutions were performed gravimetrically. Additionally, individual analytes are traceable to NIST SRMs where available and specified above.4 Ucrm - Uncertainty values in this document are expressed as Expanded Uncertainty (Ucrm) corresponding to the 95% confidence interval. Ucrm is derived from the combined standard uncertainty multiplied by the coverage factor k, which is obtained from a t-distribution and degrees of freedom. The components of combined standard uncertainty include the uncertainties due to characterization, homogeneity, long term stability, and short term stability (transport). The components due to stability are generally considered to be negligible unless otherwise indicated by stability studies. The mathematical representation of the Ucrm calculation is as follows:
k: Coverage factor derived from a t-distribution table, based on the degrees of freedom of the data set. Assume 2.0 for a Confidence interval = 95%
6 Analytical Value- For QC verification of the certified value only- not to be used in calculations. Represents the analytical data obtained by comparison to a standard as analyzed by the method described in the CoA or another acceptable method. The result may differ from the certified value and UCRM based on method uncertainty as well as the uncertainty associated with the standard used for comparison.
Traceability: The standard was manufactured under an ISO/IEC 17025:2017 certified quality system. The balance used to weigh raw materials is accurate to +/- 0.0001g and calibrated regularly using mass standards traceable to NIST. All dilutions were preformed gravimetrically. Additionally, individual analytes are traceable to NIST SRMs where available and specified above.
Homogeneity: Homogeneity was assessed in accordance with ISO 17034:2016. Completed units were sampled using a random stratified sampling protocol. The results of chemical analysis were then compared using a one-way analysis of variance approach as described by TNI EL-V3-2009 Appendix A.2. See Instructions for minimum sub-sample size.
Expiration is at end of month given on certificate and label.THIS PRODUCT WAS DESIGNED, PRODUCED AND VERIFIED FOR ACCURACY AND STABILITY IN ACCORDANCE WITH ISOISOISOISO////IEC IEC IEC IEC 17025170251702517025::::2017 2017 2017 2017 ((((ANAB Cert ATANAB Cert ATANAB Cert ATANAB Cert AT----1467146714671467) ) ) ) and ISO ISO ISO ISO 17034170341703417034::::2016 2016 2016 2016 ((((ANAB Cert ARANAB Cert ARANAB Cert ARANAB Cert AR----1470147014701470).).).).MSDS reports for components comprising greater than 1.0% of the solution or 0.1% for components known to be carcinogens are available upon request.August30,2019
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