Leafy Vegetables

WP2: Characterisation and Regeneration

Ulrike Lohwasser, IPK Gatersleben, Germany

Characterization

Descriptors

Regeneration

WP2 regeneration & characterization

Year 4

Year 3

Year 2

Year 1

WP activities

Leafy Vegetables

128314646182112797Characterization

1254334020149931Regeneration

TotalRucolaValeria-nella

ChicorySpinachLettuce

Fourth Year: Milestones & Deliverables

WP2: Characterisation and Regeneration

36

Regeneration and characterisation for all crops started

12

D07- D11

D06

Deliverable #

Start made developing minimum descriptors for the minor leafy vegetables

02

Material for regeneration and characterisation of the four crops selected

06

Minimum descriptors for the minor leafy vegetables concluded

06

Regeneration of some crops started pending the growing season

06

75% of all accessions characterised34

All accessions characterised45

M&D descriptionMonth

75% of all accessions regenerated

D12- D15 All accessions regenerated48

D1348

Characterization data entered in a fixed format to be sent to the database managers

D1145

D14

D12

D10

D09

D08

D07

Deliverable #

110 accessions of spinach characterized45

180 accessions of chicory characterized45

800 accessions of lettuce characterized45

190 accessions of minor crops characterized45

930 accessions of lettuce regenerated48

200 accessions of chicory regenerated48

M&D descriptionMonth

50 accessions of spinach regenerated

D15 70 accessions of minor crops regenerated48

Fourth Year: Milestones & Deliverables

WP2: Characterisation and Regeneration

Lettuce Spinach ChicoryValeria-

nella RucolaMonth 48 48 48 48 48 48 48 48 48 48

P0 CGN 70 60 29 15 - - - - 11 5P1 HRI 105 60 15 15 18 18 - - 4 4P2 GEVES 8 8 - - 81 60 6 8 - -P3 IPK 330 320 - - 101 100 16 15 28 24P4 Palacky - - - - - - - - - -P5 NordGen 6 6 12 7 - - 1 0 - -P6 INRA 300 300 - - - - - - - -P7 Arche Noah 61 60 12 12 16 16 8 8 - -P8 HDRA 17 17 - - - - - - - -P9 UNIBO - - - - - - - - - -P10 KIS 112 100 - - 7 7 7 9 - -P11 ProspecieRara - - - - - - - - - -

∑ 1009 931 68 49 223 201 38 40 43 33

Regeneration

Lettuce Spinach ChicoryValeria-

nella RucolaMonth 48 48 48 48 48 48 48 48 48 48

P0 CGN 765 60 - - - - - - 17 5P1 HRI 54 30 30 30 - - - - - -P2 GEVES - - - - 97 75 - - - -P3 IPK 514 500 - - - - 35 37 151 141P4 Palacky 30 30 - - - - - - - -

P5 NordGen - - 70 70 - -19 Atri - - -

P6 INRA - - - - - - - - - -P7 Arche Noah 61 60 12 12 - - - - - -P8 HDRA 17 17 - - - - - - - -P9 UNIBO - - - - - - - - - -P10 KIS 112 100 - - 8 7 7 9 - -P11 ProspecieRara - - - - 109 100 - - - -

∑ 1553 797 112 112 214 182 42 46 168 146

Characterisation

Characterisation and Regeneration (total numbers)

108%

195%

139%

100% 111%118%

95% 91%

130%115%

0%20%40%60%80%

100%120%140%160%180%200%

Lettu

ceR

egen

erat

ion

Lettu

ceC

hara

cter

isat

ion

Spi

nach

Reg

ener

atio

n

Spi

nach

Cha

ract

eris

atio

n

Chi

cory

Reg

ener

atio

n

Chi

cory

Cha

ract

eris

atio

n

Val

eria

nella

Reg

ener

atio

n

Val

eria

nella

Cha

ract

eris

atio

n

Ruc

ola

Reg

ener

atio

n

Ruc

ola

Cha

ract

eris

atio

n

Characterisation

Descriptors available for:- Cultivated Lettuce- Endive- Eruca- Leaf Chicory- Root Chicory- Spinach- Valerianella- Wild Lactuca

Partner Characterisation Data AvailabilityP0 CGN for lettuce, rucola yesP1 HRI for lettuce, spinach yesP2 GEVES for chicory yesP3 IPK for lettuce, Valerianella, rucola yesP4 Palacky for lettuce yesP5 NordGen for spinach yesP6 INRA not necessary -P7 Arche Noah for lettuce, spinach yesP8 HDRA for lettuce yesP9 UNIBO not necessary -P10 KIS for lettuce, chicory, Valerianella yesP11 ProspecieRara for chicory yes

Availability of characterisation data

Quality of characterisation data

Partner Characterisation Data QualityP0 CGN for lettuce, rucola excellentP1 HRI for lettuce, spinach excellentP2 GEVES for chicory excellent

P3 IPKfor lettuce, Valerianella, rucola excellent

P4 Palacky for lettuce excellentP5 NordGen for spinach excellentP6 INRA not necessary -P7 Arche Noah for lettuce, spinach excellentP8 HDRA for lettuce excellentP9 UNIBO not necessary -P10 KIS for lettuce, chicory,

Valerianella not as requestedP11 ProspecieRara for chicory excellent

Publication in professional paperin press 2010

Ein europäisches Projekt beschäftigt sich mit Blattgemüsen –12 Institutionen aus 10 Ländern sind beteiligt

S. Thumm, M.-L. Graichen, A. Börner & U. Lohwasser

Gemüse – das Magazin für den professionellen Gemüsebau

Gap Analysis of Eruca and DiplotaxisGenpool Concept

Genpool IBiological Species:

cultivated and spontaneous

races

Source: Harlan & De Wet, 1971

GP II GP II

All species that canbe crossed with GP I

Gene transfer possiblebut may be difficult

GP III GP III

Hybrids with GP Ianomalous, lethal or sterile

Gene transfer not possible orrequiring radical techniques

Gene pool Species Author Database

Primary Eruca sativa Mill. 495

Primary Eruca vesicaria (L.) Cav. 171

Primary Eruca pinnatifida(Desf.) Pomel 13

Secondary Diplotaxis tenuifolia (L.) DC. 49

Secondary Brassica oleracea L.

Secondary Brassica repanda (Willd.) DC.

Tertiary Brassica oleracea L.

Tertiary Brassica rapa L.

Tertiary Brassica juncea L.

Total 728

Genepool of Eruca

Reg

ion

Eruc

asa

tiva

Eru

cave

sica

ria

Eruc

api

nnat

ifida

Dip

lota

xis

tenu

ifolia

Bra

ssic

aol

erac

ea

Bras

sica

repa

nda

Bra

ssic

aol

erac

ea

Bras

sica

rapa

Bras

sica

junc

ea

Eru

cast

rum

variu

m

Eruc

astru

mca

narie

nse

Eru

cast

rum

card

amin

oide

s

Europe 144 32East 3North 1Middle 17 5 2South 106 106 28 1 4Southeast 5 1 1West 13 1Asia 291 4Central 12East 6Near East 27 2 4South 234 5West 12 4Africa 29East 1North 28 17 8 31SouthAmerica 6 5North 6 3 3South 1 2

unknown 25 26 2 7 2

Genepool of Eruca

Eruca

Secondary genepool (Diplotaxis tenuifolia, Brassica repanda, B. oleracea)

Primary Genepool (natural distribution, naturalized elsewhere, except tropics)

Tertiary genepool (B. oleracea Europe, B. junceaEast Asia, B. rapa widely distributed)

B. junceaB. oleracea

Eruca

Primary genepoolSecondary genepool

Diplotaxistenuifolia

Gene pool Species Authority Database

Primary Diplotaxis tenuifolia (L.) DC. 49

Secondary Brassica rapa L.

Secondary Brassica juncea (L.) Czern.

Secondary Brassica nigra (L.) W. D. J. Koch

Tertiary Erucastrum virgatum C. Presl 38

Tertiary Brassica oleracea L.

Tertiary Brassica elongata Ehrh.

Total 87

Genepool of Diplotaxis tenuifolia

Genepool of Diplotaxis muralis

Diplotaxismuralis

Gene pool Species Author Database

Primary Diplotaxis muralis (L.) DC. 49

Secondary Brassica juncea L.

Secondary Brassica napus L.

Secondary Brassica rapa L.

Tertiary Diplotaxis harra (Forssk.) Boiss. 31

Tertiary Erucastrum gallicum(Willd.) O. E. Schulz

Total 80