learning objectives (cont.) handling a...

BIOL 3702L - Bright-Field Microscopy Fall 2020

Dr. Cooper 1

BIOL 3702L:Bright-Field Microscopy

Copyright © C. R. Cooper, Jr.

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Learning Objectives

Upon completion of this exercise, a student should be able to:• Understand the principles of light microscopy, in

particular the concepts of resolution, magnification, and depth of field/focus;• Effectively use the microscope to view slides of

different cell types;• Correctly employ the oil immersion lens to view

eukaryotic and prokaryotic cells;

Copyright © C. R. Cooper, Jr.https://www.shutterstock.com/video/clip-537799-stock-footage-researchers-working-in-lab-with-microscope-close-up.html

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Learning Objectives (cont.)

Upon completion of this exercise, a student should be able to: (cont.)• Appreciate the general size

difference between pro-karyotes and eukaryotes; and• Recognize the various shapes

of some common types of bacteria

Copyright © C. R. Cooper, Jr.https://www.shutterstock.com/video/clip-537799-stock-footage-researchers-working-in-lab-with-microscope-close-up.html

https://www.the-scientist.com/image-of-the-day/image-of-the-day-ciliated-protozoan-38426

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Handling a Microscope

• Always carry a microscope with one hand on the arm and the other under the base.• Always use lens paper to clean the

objectives and eye pieces of a microscope. Never use paper towels, Kimwipes, etc.• Before storing the microscope, always clean

it appropriately and remove all slides.• Always store the microscope with the low

power objective (4X) in place.Copyright © C. R. Cooper, Jr.

https://www.shutterstock.com/video/clip-537799-stock-footage-researchers-working-in-lab-with-microscope-close-up.html

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Typical Bright-Field Microscope

Copyright © C. R. Cooper, Jr. https://www.dotmed.com

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Parts of the Microscope


http://faculty.ccbcmd.edu/courses/bio141/labmanua/lab14/focusing/microscope.html

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Dr. Cooper 2

Parts of the Microscope (cont.)

Copyright © C. R. Cooper, Jr. http://binoculas.net/olympus-microscope-ch2/

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How to Focus a Microscope


https://youtu.be/I5AWck2JL58

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How to Use Oil Immersion


https://youtu.be/BgiuCJRHFjY

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Today’s Exercise

• Read/follow the procedure outlined in today’s exercise.• Be sure to record

your observations as indicated using the report sheets attached to the instructions.


https://designmanagementandculturesctslcc.wordpress.com/2016/03/11/attention-read-and-follow-all-instructions/

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Today’s Exercise (cont.)

• Prepare a wet mount of newspaper print and exam it under 40X, 100X, 400X, and 1000X (oil immersion) power


http://www.deftstudios.com/bioweb/images/blab03G.gif

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• Prepare a wet mount of the yeast, Saccharomyces kudriavzevii, and examine it under 40X, 100X, 400X, and 1000X (oil immersion) power


https://eurekabrewing.files.wordpress.com/2012/02/cut1056-6.jpg

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https://youtu.be/I5AWck2JL58

https://youtu.be/BgiuCJRHFjY


Dr. Cooper 3



Modified from http://library.open.oregonstate.edu/microbiology/chapter/cell-structure/

• Examine prepared slides of stained bacteria under 1000X power (oil immersion) • Coccus (round ball-like cell; pl. “cocci”)• Bacillus (rod-like cell; pl. “bacilli”)• Spirillum (wavy/curved cell; pl. “spirilla”)

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Lab Report Expectations


• From the exercise handout, print the report pages 15-17.• Record/draw your

observations as indicated.• Answer Discussion Questions

Where appropriate, USE CLEAR, CONCISE, AND GRAMMATICALLY SOUND SENTENCES! https://davidkanigan.com/2016/06/21/sorry-i-had-to-laugh/

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Lab Report Expectations


• Scan your report as a PDF• Submit your report PDF via

Blackboard as indicated by your laboratory instructor.

https://davidkanigan.com/2016/06/21/sorry-i-had-to-laugh/

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Questions?


https://www.huffingtonpost.com/barbara-jacoby/asking-questions-is-really-hard_b_7052722.html

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learning objectives (cont.) handling a...

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